##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309638
Sequences flagged as poor quality	0
Sequence length	42
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.212712909914156	30.0	25.0	31.0	16.0	35.0
2	25.10057874033549	28.0	20.0	31.0	10.0	35.0
3	27.63823238749766	30.0	26.0	31.0	17.0	35.0
4	27.89877534411151	30.0	26.0	31.0	17.0	35.0
5	24.462808182458225	27.0	19.0	30.0	8.0	34.0
6	27.396695496030848	30.0	26.0	31.0	16.0	37.0
7	27.073007834955657	29.0	24.0	31.0	16.0	37.0
8	26.880673560738668	30.0	23.0	31.0	13.0	37.0
9	25.469984304252062	27.0	26.0	27.0	18.0	31.0
10	25.469570918298142	27.0	26.0	27.0	18.0	31.0
11	25.02134104987114	27.0	25.0	27.0	16.0	31.0
12	27.192809022148445	30.0	25.0	31.0	17.0	35.0
13	30.55534850373662	31.0	30.0	31.0	28.0	34.0
14	30.474799604699683	31.0	30.0	31.0	27.0	34.0
15	30.7054463599429	31.0	31.0	31.0	27.0	34.0
16	34.54816915236502	35.0	35.0	37.0	32.0	37.0
17	34.584941124797346	35.0	35.0	37.0	32.0	37.0
18	34.3646968395352	35.0	35.0	37.0	30.0	37.0
19	34.347056885782756	35.0	35.0	37.0	30.0	37.0
20	34.35507269779549	35.0	35.0	37.0	31.0	37.0
21	35.82641019513109	37.0	35.0	39.0	31.0	39.0
22	35.66467294065974	37.0	35.0	39.0	31.0	39.0
23	35.98094549118648	37.0	35.0	39.0	32.0	39.0
24	36.17975829840007	37.0	35.0	39.0	32.0	39.0
25	22.91147727346127	32.0	2.0	37.0	2.0	39.0
26	29.514084188633177	34.0	17.0	38.0	17.0	40.0
27	34.04813039743184	36.0	32.0	39.0	27.0	40.0
28	36.04517533377686	37.0	34.0	39.0	32.0	40.0
29	36.74105245480206	38.0	36.0	39.0	32.0	40.0
30	37.114992345900696	38.0	36.0	40.0	32.0	40.0
31	37.40903248309316	39.0	36.0	40.0	33.0	41.0
32	37.48402650837429	39.0	36.0	40.0	33.0	41.0
33	37.47376291023711	39.0	36.0	40.0	33.0	41.0
34	37.47568127942953	39.0	36.0	40.0	33.0	41.0
35	37.37547071095925	39.0	36.0	40.0	32.0	41.0
36	37.37980480431988	39.0	36.0	40.0	32.0	41.0
37	37.299720964481104	39.0	36.0	40.0	32.0	41.0
38	37.11287697246462	38.0	36.0	40.0	32.0	41.0
39	37.0040789567172	38.0	36.0	40.0	32.0	41.0
40	37.023152842997305	38.0	36.0	40.0	32.0	41.0
41	36.94580445552548	38.0	36.0	40.0	32.0	41.0
42	36.96512378971573	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.5194273390500435
1101	2	6.073824774205558
1101	3	4.93406591443312
1101	4	5.14372383278522
1101	5	7.36150848031723
1101	6	8.187710985357441
1101	7	6.932012822489693
1101	8	6.670194444665814
1101	9	5.802670652809216
1101	10	5.492099136334524
1101	11	3.4179549445473327
1101	12	7.5534864108288815
1101	13	-0.06422983455770392
1101	14	0.8535961441260547
1101	15	1.482918084016422
1101	16	0.6997712272031862
1101	17	0.17218639162696547
1101	18	-0.12291742022812002
1101	19	-0.14871243009699953
1101	20	-0.22283301599178884
1101	21	-0.1949419984577574
1101	22	0.8538201661008102
1101	23	0.28048725199683133
1101	24	0.24806596363650613
1101	25	11.863162700556671
1101	26	6.7906278889678475
1101	27	2.9119466210853133
1101	28	1.223792391576616
1101	29	0.6925367755071363
1101	30	0.3467024879596039
1101	31	0.013622674785580102
1101	32	-0.19848054630161016
1101	33	-0.0397947315148528
1101	34	-0.00858500803866491
1101	35	-0.13533321957174138
1101	36	-0.009064417795457302
1101	37	-0.30679855617179896
1101	38	-0.3211005665525377
1101	39	-0.24948240530872567
1101	40	-0.3769615265259745
1101	41	-0.39076523690427223
1101	42	-0.36904606572972654
1102	1	4.193250191572282
1102	2	3.258234178945248
1102	3	2.358862207273827
1102	4	1.7204483460650621
1102	5	4.148418351561865
1102	6	4.915380948659049
1102	7	3.146048401266828
1102	8	3.400915350307411
1102	9	4.263807680861714
1102	10	3.3739116635473145
1102	11	3.820452301019312
1102	12	5.733361387503635
1102	13	-0.19087659701338566
1102	14	1.1373337817450135
1102	15	1.6229690885570704
1102	16	0.9095087404208257
1102	17	0.23030979773507454
1102	18	-0.09177977016903327
1102	19	-0.08707779676988281
1102	20	-0.059857958456177585
1102	21	-0.15451156989521309
1102	22	0.8355114020279117
1102	23	0.42334972633906176
1102	24	0.3004140385260996
1102	25	11.999960610614515
1102	26	6.7679744088409635
1102	27	2.9229685779180556
1102	28	1.2520812514385327
1102	29	0.8688600945343197
1102	30	0.5697727125039833
1102	31	0.11894705122396942
1102	32	0.002962508994770019
1102	33	0.01552653489057576
1102	34	0.07168183278984941
1102	35	0.020034697328178197
1102	36	0.09213605492954713
1102	37	-0.2101667218743586
1102	38	-0.35997597863190123
1102	39	-0.07784279592886634
1102	40	-0.08789640243565344
1102	41	-0.10938189424084044
1102	42	-0.0026624120629961112
1103	1	1.6735128966313617
1103	2	-0.44769484862539954
1103	3	-0.27774251147497964
1103	4	0.4407840884840901
1103	5	0.9930533559594004
1103	6	1.8589299525212333
1103	7	-2.3315298940182494
1103	8	-0.8524745774200149
1103	9	2.326287127154398
1103	10	1.4833644569395368
1103	11	-1.722351033461205
1103	12	1.447328348614107
1103	13	-0.3366148097012349
1103	14	1.4156806900850398
1103	15	1.7123997030651203
1103	16	1.041765633082676
1103	17	0.4637865290264216
1103	18	0.1942016454556068
1103	19	0.10573700954727627
1103	20	-0.005919294542749753
1103	21	0.046054374045745305
1103	22	0.9506905538767896
1103	23	0.4798523457417616
1103	24	0.41691818750701515
1103	25	12.04638713350083
1103	26	7.127658550938776
1103	27	3.3332741111672632
1103	28	1.4951732938078521
1103	29	0.7850870074630762
1103	30	0.45148278095260963
1103	31	0.5360859751656903
1103	32	0.11891218587137331
1103	33	0.16643089801244315
1103	34	0.23648050205679993
1103	35	0.10557444724214093
1103	36	0.2512886685963309
1103	37	-0.20996214078759579
1103	38	0.015401635990862417
1103	39	-0.057302472423074846
1103	40	0.12408784290869335
1103	41	0.08327230636334804
1103	42	0.06616669581050871
1104	1	1.269507266195241
1104	2	1.1893445267059057
1104	3	-0.6266270458422802
1104	4	-0.6051806001841626
1104	5	1.3455214365882
1104	6	1.1038439970445637
1104	7	-1.1800401910058866
1104	8	-0.30784900340737664
1104	9	2.0224460817515606
1104	10	1.0244203821000006
1104	11	0.8885175347681269
1104	12	2.620859342814917
1104	13	0.19050201390689026
1104	14	1.8614758303595345
1104	15	1.7974677308837634
1104	16	0.72774572802183
1104	17	0.06055319747989785
1104	18	-0.004368509659329334
1104	19	0.05297847306359671
1104	20	-0.19927792116105536
1104	21	-0.35129241197982
1104	22	0.7013094849277692
1104	23	0.20956361713632532
1104	24	-0.02801531338936769
1104	25	11.70113180831138
1104	26	6.291503050840504
1104	27	2.7630673772768404
1104	28	1.0087044071293079
1104	29	0.45256965059150644
1104	30	0.10607656960477385
1104	31	-0.09598408366005629
1104	32	0.04779517167020941
1104	33	0.07945495442798745
1104	34	0.056209264333674014
1104	35	-0.23147346506917899
1104	36	-0.3205639156269342
1104	37	-0.30929486660560457
1104	38	-0.532400488724484
1104	39	-0.3937210306264163
1104	40	-0.4697722816518066
1104	41	-0.2766308882539903
1104	42	-0.45206372720678445
1105	1	0.5803582118694379
1105	2	0.933251519141649
1105	3	-1.457607328739023
1105	4	-0.8041199743087581
1105	5	-0.870011539286164
1105	6	-2.0250326606571996
1105	7	-2.0123661571970146
1105	8	-5.3209552005632474
1105	9	1.401532647093962
1105	10	0.6569730295897322
1105	11	1.0721388632797435
1105	12	3.088008560138981
1105	13	0.15711011492032156
1105	14	1.7002932969496278
1105	15	1.375537262908182
1105	16	0.6212945981217004
1105	17	0.1670801405233746
1105	18	-0.14419827124594775
1105	19	-0.1855372572159837
1105	20	-0.2506128958489384
1105	21	-0.46477331559667334
1105	22	0.37518029554085075
1105	23	-0.22561898829606264
1105	24	-0.20847144316630306
1105	25	11.613778009219999
1105	26	6.601702003481925
1105	27	2.7452114866903727
1105	28	1.0755507509457019
1105	29	0.48619846565102876
1105	30	0.0709883515358527
1105	31	-0.33482581045602444
1105	32	-0.47060373055547444
1105	33	-0.2652787803359402
1105	34	-0.44071366971078874
1105	35	-0.4336633277147115
1105	36	-0.45738389542272984
1105	37	-0.4847605962218182
1105	38	-0.25179140593085236
1105	39	-0.5996750158390611
1105	40	-0.5071348940210996
1105	41	-0.4268243065359485
1105	42	-0.5764276075508974
1106	1	-4.573693748033477
1106	2	-4.70410097180735
1106	3	-1.5754147860623036
1106	4	-6.042704643356629
1106	5	-0.5298774683738259
1106	6	0.8968223019373944
1106	7	-1.461125744779121
1106	8	-5.005189314402035
1106	9	-0.5229437987039702
1106	10	0.18693650161059594
1106	11	0.7857978436820652
1106	12	3.1499954338245253
1106	13	0.06711305767695563
1106	14	1.6936248484303214
1106	15	1.6501045561930283
1106	16	0.6899202227825398
1106	17	0.3589667714989915
1106	18	-8.482262946643004E-4
1106	19	0.010210054481341047
1106	20	-0.2965441973303058
1106	21	-0.3528432722112598
1106	22	0.8414506349837794
1106	23	0.17228490505195282
1106	24	-0.06664407228987557
1106	25	11.720446187761638
1106	26	6.726215706198715
1106	27	2.569788094204931
1106	28	1.1616250326026147
1106	29	0.4977454107186716
1106	30	0.3227155336978882
1106	31	-0.4397742312216266
1106	32	-0.20372000183984085
1106	33	0.07707180727046392
1106	34	-0.15969660982164413
1106	35	-0.54240763456054
1106	36	-0.4340767229258944
1106	37	-0.4392477601333624
1106	38	-0.40906959902427786
1106	39	-0.3617166949921966
1106	40	-0.23369070785529544
1106	41	-0.025831868610566744
1106	42	-0.09565136774592986
1107	1	2.947530609502852
1107	2	1.1983673100541132
1107	3	0.7261692514261675
1107	4	-4.522726578100762
1107	5	-20.63491498177147
1107	6	-23.21143064554385
1107	7	-9.353673540088078
1107	8	-5.3359147811384915
1107	9	0.8784183987585799
1107	10	1.4759423342584697
1107	11	2.285922472910112
1107	12	4.162296338632721
1107	13	0.013572341308151437
1107	14	1.5587105933392174
1107	15	1.4000671674246128
1107	16	0.5853344903365638
1107	17	-0.3417579474620993
1107	18	-0.5114292477558209
1107	19	0.23449281327393123
1107	20	0.19165029904298336
1107	21	0.23704555851732323
1107	22	1.0872724401135159
1107	23	0.44106964544927507
1107	24	0.38426135905188374
1107	25	12.240633006974477
1107	26	6.8103404351301435
1107	27	2.637343367267569
1107	28	0.4580805773569665
1107	29	0.31459652864284493
1107	30	-0.161032814964841
1107	31	-0.16460535861762082
1107	32	-0.577202640988375
1107	33	-0.3192055176877844
1107	34	-0.3435776886122355
1107	35	-0.818514342105594
1107	36	-0.39244432929694284
1107	37	-0.2617508135494475
1107	38	-0.09485127354258793
1107	39	-0.3594235171961415
1107	40	-0.23048150523309374
1107	41	-0.03583959562270422
1107	42	-0.03694788560530071
1113	1	-10.929213576543662
1113	2	-5.847562922504025
1113	3	-0.5447609811319722
1113	4	1.7226222591085403
1113	5	2.515085018228529
1113	6	-23.21143064554385
1113	7	-9.349603772646216
1113	8	0.5704805676987164
1113	9	-20.662279275660026
1113	10	-21.224639061090368
1113	11	-12.50128682941547
1113	12	-21.570261800902163
1113	13	0.3286886203779211
1113	14	1.833710593339216
1113	15	0.5012299581222877
1113	16	1.0754507694063307
1113	17	-0.528385854438838
1113	18	-0.08235948031396134
1113	19	0.042632348157653155
1113	20	0.52130146183368
1113	21	1.299836256191739
1113	22	2.0297143005786324
1113	23	1.9776975524260152
1113	24	1.4069357776565354
1113	25	13.20865626278843
1113	26	8.544061365362701
1113	27	4.028041041686173
1113	28	2.388313135496503
1113	29	-0.6144732387990146
1113	30	1.116874161779343
1113	31	0.46097603673121057
1113	32	0.8297741031976713
1113	33	-0.13664737815290096
1113	34	-0.02904280489131139
1113	35	1.1309042625455703
1113	36	0.23778822884259654
1113	37	0.9847608143575286
1113	38	0.48712547064345557
1113	39	1.6859253200131619
1113	40	2.0113789598831815
1113	41	1.1420673811214783
1113	42	2.341540486487723
1114	1	-8.430922978253061
1114	2	-2.500127025068128
1114	3	-0.0011712375422270327
1114	4	-6.2983179118316315
1114	5	-2.087906434762921
1114	6	0.3099368758236736
1114	7	0.27048169743925143
1114	8	1.7593694565876064
1114	9	-4.431510044890796
1114	10	1.142882306430998
1114	11	1.5384567603281205
1114	12	-21.570261800902163
1114	13	0.119714261403562
1114	14	-27.066289406660783
1114	15	-13.37825722136489
1114	16	-5.46557487161931
1114	17	-2.1343687604217436
1114	18	-0.48022272817721046
1114	19	-0.23300867748337595
1114	20	-0.09792930739708794
1114	21	0.10710121345669421
1114	22	1.278859599723937
1114	23	1.1909454156738803
1114	24	0.7603545810753332
1114	25	11.930878485010652
1114	26	6.959018630319967
1114	27	3.2592376228827504
1114	28	1.9737832209665882
1114	29	0.7641592398334609
1114	30	0.4711476660528504
1114	31	-0.04842567267049702
1114	32	-0.1894566660330952
1114	33	-0.37938242088794993
1114	34	0.08121360536510025
1114	35	-0.5511470195057129
1114	36	0.10402754508191236
1114	37	-0.39472636512965664
1114	38	0.2533647868827771
1114	39	-0.31450203041418945
1114	40	-0.06426206575784477
1114	41	0.06642635548045206
1114	42	0.22102766597490842
1115	1	-8.562546909876996
1115	2	-3.597562922504025
1115	3	-1.0336498700208594
1115	4	-0.23848885200257186
1115	5	2.4095294626729746
1115	6	2.9219026877894834
1115	7	2.8448406717982273
1115	8	2.6482583454764956
1115	9	-20.662279275660026
1115	10	-21.224639061090368
1115	11	-8.740175718304359
1115	12	-21.570261800902163
1115	13	-0.09908915739985957
1115	14	1.6670439266725516
1115	15	-27.19877004187771
1115	16	-14.824549230593668
1115	17	-4.606163632216617
1115	18	-1.1879150358695156
1115	19	-1.0518120962867883
1115	20	0.5601903507225643
1115	21	0.46094736730285035
1115	22	0.6908254116897439
1115	23	0.7499197746482409
1115	24	0.023602444323202576
1115	25	12.375322929455095
1115	26	6.938505809807147
1115	27	2.811374375019504
1115	28	1.332757579940946
1115	29	1.1744156500898697
1115	30	0.011318606223788663
1115	31	0.9943093700645491
1115	32	0.8908852143087813
1115	33	0.6411303996248705
1115	34	0.4709571951086886
1115	35	0.4197931514344617
1115	36	0.454454895509258
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2206	37	0.16245312204983264
2206	38	-0.2105668370488516
2206	39	0.14054070462854895
2206	40	-0.015544117039894445
2206	41	0.024375073429176553
2206	42	-0.03769028274304276
2207	1	-1.0745210199093584
2207	2	0.03609403542477807
2207	3	-4.940553861390871
2207	4	-1.3215524981730162
2207	5	-1.071808185654966
2207	6	1.3338767978218478
2207	7	-2.740056847079874
2207	8	-0.9353446750197278
2207	9	1.4089181353432103
2207	10	1.2316716185212826
2207	11	1.7415934294842046
2207	12	1.4297381990978373
2207	13	0.25700577248148093
2207	14	2.037270463889378
2207	15	1.7332687930737443
2207	16	0.8388811901183075
2207	17	0.2538147928103527
2207	18	0.09288323813264299
2207	19	0.27159674945215073
2207	20	0.29266068513464916
2207	21	0.3294479066771743
2207	22	1.099455400902258
2207	23	0.5526166462771513
2207	24	0.2886509880125203
2207	25	6.362377945636325
2207	26	4.025776575718687
2207	27	1.8544164462169164
2207	28	0.5485073102537825
2207	29	0.35510604922687605
2207	30	0.09956024592173662
2207	31	0.24026406262117916
2207	32	0.060356627469516866
2207	33	-0.06965708689077132
2207	34	0.12079538281095381
2207	35	0.12896251497275557
2207	36	-0.21544801711210226
2207	37	-0.1650773733447366
2207	38	-0.015948963013499906
2207	39	-0.05145332076353526
2207	40	-0.12114531196147738
2207	41	-0.02605559622480058
2207	42	0.041540486487726014
2208	1	1.3276491685543768
2208	2	-21.397562922504026
2208	3	-9.725153137994717
2208	4	-2.2058091134404805
2208	5	2.4631242339148045
2208	6	3.9022948446522285
2208	7	3.1219648548047623
2208	8	-24.129519432301283
2208	9	3.439681508653699
2208	10	3.308694272242967
2208	11	-21.05128682941547
2208	12	-6.738889251882554
2208	13	0.7179043066524287
2208	14	2.325867456084314
2208	15	2.1071123110634637
2208	16	1.5127056713671152
2208	17	1.1127906161493968
2208	18	0.665679735372315
2208	19	1.1259656814909889
2208	20	0.6595367559513221
2208	21	0.7302284130544834
2208	22	1.724812339794319
2208	23	1.3786779445828827
2208	24	1.137327934519277
2208	25	-22.29134373721157
2208	26	-13.027507262088282
2208	27	-4.474900134784416
2208	28	-1.096000589993693
2208	29	-0.22035559174019426
2208	30	0.425697691191111
2208	31	0.45705446810376316
2208	32	0.7797741031976742
2208	33	0.9300192885137619
2208	34	0.9297807245204552
2208	35	0.6563944586239998
2208	36	1.1721019543327884
2208	37	0.9298588535732151
2208	38	1.0037921373101284
2208	39	1.1849449278563
2208	40	1.1535358226282852
2208	41	1.0685379693567754
2208	42	1.3984032315857675
2209	1	-2.012638990908304
2209	2	-4.03844690040458
2209	3	-4.717136671739706
2209	4	-5.24892470221743
2209	5	-3.679113876799093
2209	6	-3.327452744991362
2209	7	-8.127780568226328
2209	8	-6.472060868765372
2209	9	-2.850124579527428
2209	10	-0.20806447545501072
2209	11	-2.6203476028961354
2209	12	-0.5702618009021627
2209	13	0.1029980126431127
2209	14	1.4751470574276162
2209	15	1.0829979139233927
2209	16	0.4793181727212499
2209	17	0.313050609649558
2209	18	-0.950591524512852
2209	19	-1.16316875681472
2209	20	-0.7043891459011249
2209	21	-0.7175670587253862
2209	22	-0.34072768837164347
2209	23	-0.8546228895629326
2209	24	-0.8496940565976132
2209	25	-4.318968046603835
2209	26	-3.3390878059080187
2209	27	-2.493229676545873
2209	28	-1.4652780247244905
2209	29	-0.70894837692056
2209	30	-1.0276009763421996
2209	31	-1.1622283831582862
2209	32	-1.1495076647581328
2209	33	-1.502393234506492
2209	34	-2.224070429200701
2209	35	-1.2248968424268014
2209	36	-1.9912172960192862
2209	37	-0.9113717823275564
2209	38	-0.8750292254891363
2209	39	-0.638107829158109
2209	40	-1.928123802547752
2209	41	-1.6703635581050378
2209	42	-1.8866363090923812
2215	1	-13.08690588423597
2215	2	-4.589870614811719
2215	3	-1.2255302119012015
2215	4	-0.8004546639683845
2215	5	2.2689311720746836
2215	6	3.8078001236869206
2215	7	2.550396227353783
2215	8	2.7743267215448704
2215	9	-20.662279275660026
2215	10	-10.474639061090368
2215	11	-2.993594521723164
2215	12	-21.570261800902163
2215	13	-0.0020806103913102447
2215	14	-27.066289406660783
2215	15	-13.198770041877712
2215	16	-4.747626153670591
2215	17	-2.0360781621311475
2215	18	-0.8092825572370401
2215	19	0.06186311738841965
2215	20	0.32514761567983186
2215	21	0.7652208715763535
2215	22	1.3450989159632485
2215	23	1.02000524473371
2215	24	0.3030896238103793
2215	25	10.03557933971151
2215	26	5.8286767499780865
2215	27	2.139579503224631
2215	28	0.5613900585734228
2215	29	0.5739882996625241
2215	30	0.5053357002408845
2215	31	-0.1851778094226333
2215	32	-0.6509951275715622
2215	33	-0.1443396858452104
2215	34	-0.25211972796822835
2215	35	0.4232119548532651
2215	36	0.0954805365349003
2215	37	0.7847608143575258
2215	38	1.0525100860280716
2215	39	-0.3102285261406834
2215	40	0.6729174214216442
2215	41	1.476682765736868
2215	42	1.003078948026186
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	17.0
25	250.0
26	1590.0
27	5732.0
28	13441.0
29	22909.0
30	34022.0
31	43261.0
32	49927.0
33	51175.0
34	40723.0
35	27983.0
36	12155.0
37	6230.0
38	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	92.88464429758334	4.062322279528563	1.6427091300113088	1.4103242928768
2	3.058436589217969	92.20639236693422	1.501538661933992	3.233632381913802
3	1.5883063448284769	2.234544855605578	0.9863130494319172	95.19083575013403
4	1.0350796736834627	1.5508432427544423	95.85483693861865	1.55924014494345
5	2.343017806935333	90.91566780118056	0.9276706292441506	5.813643762639964
6	1.1461577096936213	2.9124393102293653	1.0333165913276412	94.90808638874937
7	94.60752175949101	3.269010286304843	0.5745474510310526	1.5489205031730908
8	3.6081097803221245	2.6875207596569037	92.03057124062967	1.6737982193913106
9	32.41534358921122	21.750485002238474	27.737586213608612	18.096585194941703
10	34.26770898775251	21.61801566072319	25.54194003847175	18.572335313052545
11	30.5198402095783	19.61994382571635	22.229245817883232	27.630970146822115
12	31.93664253638267	24.781573711341885	19.904390669623524	23.37739308265192
13	28.480977900962976	26.55696292491625	21.1489819454001	23.813077228720672
14	23.28440605312723	27.93661102812236	23.697148795219846	25.081834123530555
15	23.90590685775091	23.297529294948298	27.779987903604013	25.016575943696772
16	26.186708349750354	24.894554285972653	24.885188510454142	24.03354885382285
17	20.6782606448612	26.891396897792536	25.866802762398816	26.563539694947448
18	20.91539152171245	27.197243232419794	20.76263249342781	31.12473275243995
19	28.235875441644758	22.883496211705282	21.814828929265786	27.065799417384174
20	25.875054095427565	27.858014843139404	20.854029544177394	25.41290151725563
21	22.86314987178576	28.994826216420467	21.415007201958417	26.727016709835354
22	24.486598126213465	27.213556936789026	22.32223134357531	25.9776135934222
23	28.009159082541547	27.427512127064507	22.44976391786538	22.113564872528567
24	24.43918382110723	27.195628443537288	25.919622268584607	22.445565466770876
25	20.259728855563633	27.42959172224038	25.35039491079748	26.96028451139851
26	22.6849205349559	28.28542177351897	26.378325307702987	22.651332383822137
27	27.631154000180857	26.02119908537767	25.11723443010503	21.230412484336448
28	26.285736432105622	27.070872143346207	25.8955908380379	20.747800586510266
29	22.087728250408542	31.87334887836764	25.06378416085881	20.97513871036501
30	24.757297230960024	29.56032528307249	25.030842467655777	20.651535018311705
31	24.427557341153218	31.424437569032225	23.588190080028937	20.55981500978562
32	21.635264405531622	31.422176864596725	25.115780362875356	21.8267783669963
33	20.780072213358824	31.653414632570936	26.00326833269818	21.563244821372056
34	23.627267970985475	31.205149238788522	27.854462307597906	17.3131204826281
35	22.74107183226865	32.85029615228105	24.976908518980228	19.43172349647007
36	21.36527170437737	32.96849869848016	25.90185959087709	19.76437000626538
37	21.197656618373713	35.40004779775092	24.445320018860734	18.95697556501463
38	22.392277433648324	37.05585231786796	22.3632112337633	18.188659014720415
39	19.74079408858086	36.65732242166659	24.89261653931365	18.7092669504389
40	19.915837203444024	32.6949534617844	24.469864809874757	22.919344524896815
41	16.71177310278454	33.027277013803214	27.630006652930195	22.630943230482046
42	17.856335462701605	34.382084886221975	26.874285455919495	20.887294195156926
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0
7	4.0
8	14.5
9	25.0
10	25.0
11	72.5
12	120.0
13	257.5
14	395.0
15	644.0
16	893.0
17	893.0
18	1411.5
19	1930.0
20	2396.0
21	2862.0
22	2862.0
23	3280.5
24	3699.0
25	4215.5
26	4732.0
27	5427.5
28	6123.0
29	6123.0
30	7021.0
31	7919.0
32	8783.5
33	9648.0
34	9648.0
35	11348.0
36	13048.0
37	13093.5
38	13139.0
39	12903.5
40	12668.0
41	12668.0
42	15264.5
43	17861.0
44	21640.0
45	25419.0
46	29656.5
47	33894.0
48	33894.0
49	36885.5
50	39877.0
51	39774.0
52	39671.0
53	39671.0
54	35393.5
55	31116.0
56	24959.0
57	18802.0
58	14518.5
59	10235.0
60	10235.0
61	8007.0
62	5779.0
63	4978.0
64	4177.0
65	3354.0
66	2531.0
67	2531.0
68	2069.0
69	1607.0
70	1137.0
71	667.0
72	667.0
73	564.0
74	461.0
75	344.5
76	228.0
77	154.5
78	81.0
79	81.0
80	52.0
81	23.0
82	13.5
83	4.0
84	4.0
85	2.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	1.1884846175211052
2	0.8245112034052667
3	0.0
4	0.0
5	1.7901549551411648
6	3.5486600481853
7	9.68873329500901E-4
8	0.8241882456287665
9	0.44955722488841815
10	0.2712845322602523
11	0.8836124765048218
12	0.8251571189582674
13	0.024867748790523128
14	0.54838230449751
15	0.14694578830763666
16	0.0
17	0.016793804378015618
18	0.0
19	0.0
20	0.0
21	0.0
22	0.8610054321498007
23	0.0
24	0.0
25	33.79914609963893
26	0.0016147888825015018
27	6.459155530006008E-4
28	0.0032295777650030036
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
42	309638.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.27874463761554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1437788987768	40.798073381827344
2	8.359723577259926	8.406328139642111
3	3.574585809847254	5.391770613556625
4	1.9809036242468658	3.9838938990094115
5	1.2377239100470643	3.111560220256368
6	0.7854237543636513	2.369407222678037
7	0.5531274962038887	1.9467389293575283
8	0.35937637075472656	1.445519421917595
9	0.2728865579138198	1.2348354204348146
>10	1.4491115938513228	13.944895589066036
>50	0.16803812492408432	5.884965724020031
>100	0.11334336269389217	10.046718593033408
>500	6.589730389179777E-4	0.16764962596296223
>1k	0.0013179460778359553	1.2676432192377343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACTACGTTGCCAAGGAAGGAAGGGAGGATGGAAGGAAGGAA	2320	0.7492620414806969	No Hit
GACTACGTTGCCAAGGAAGGAAGGNAGGATGGAAGGAAGGAA	1506	0.48637441140945237	No Hit
GACTACGTTATTCCCAGCCCACTCAACCAACCCACCAGTCCT	506	0.16341663490915198	No Hit
GACTACGGGGTAGCCGATCAGCGACTCTTCGATGAGCAGCTC	484	0.1563115638261454	No Hit
GACTACGTATCGCTGCTGCAGGGCAGCATCGAGAATATGCAG	437	0.14113254833063124	No Hit
GGCGACGTTCGATCCGACGATCTCGATAATAGCGCCAGTGAC	386	0.12466170172911593	No Hit
GACTACGGGGTAGCCGATCAGCGANTCTTCGATGAGCAGCTC	378	0.12207803951711353	No Hit
GACTACGTGAGAAAAATGGTAAGTATGTGAGATGATGGATTT	374	0.12078620841111233	No Hit
GACTACGTTAAGCAGTCCGACGATCGAAAGACTACGTAGTCA	373	0.12046325063461204	No Hit
GACTACGTCGTTCAGTCCGACGATCCGGCGATCGTCGGACTG	357	0.11529592621060722	No Hit
GACTACGTGCGATTCGACGATCGTCGGACTGTAGAACTCTGA	344	0.11109747511610331	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GACTACGTCAACGGAAAGTTCAACATCAGACAGTCCAACCTC	331	0.10689902402159941	No Hit
GACTACGTGGACTTCTACAGTCCGACGATCGTAGGACTGTAG	326	0.1052842351390979	No Hit
TGCTACGTCAGTTCCGACGATCCAGTAGAAAAACAAAAAAAA	310	0.1001169107150931	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	6.459155530006007E-4	0.0	0.0
12	0.0	0.0	0.047151835369043854	0.0	0.0
13	0.0	0.0	0.11852550397561022	0.0	0.0
14	0.0	0.0	0.20798480806619343	0.0	0.0
15	0.0	0.0	0.3620356674568367	0.0	0.0
16	0.0	0.0	0.3965921495423688	0.0	0.0
17	0.0	0.0	0.4127400383673838	0.0	0.0
18	0.0	0.0	0.43179454718090154	0.0	0.0
19	0.0	0.0	0.6249232975280812	0.0	0.0
20	0.0	0.0	0.8167602167692596	0.0	0.0
21	0.0	0.0	1.04283066031947	0.0	0.0
22	0.0	0.0	1.4277963299078278	0.0	0.0
23	0.0	0.0	1.7920927018001667	0.0	0.0
24	0.0	0.0	2.050781880776907	0.0	0.0
25	0.0	0.0	2.1444396359619944	0.0	0.0
26	0.0	0.0	2.5067982611953314	0.0	0.0
27	0.0	0.0	2.6414716539959566	0.0	0.0
28	0.0	0.0	2.8019816689166057	0.0	0.0
29	0.0	0.0	2.9960792925932864	0.0	0.0
30	0.0	0.0	3.5370335682312892	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGTAG	15	0.007920338	50.78146	21
GCCCTGA	15	0.007920338	50.78146	21
AAGACTC	20	3.86806E-4	50.781456	21
CAACATC	85	0.0	47.794315	21
TCTTTGA	60	0.0	46.54967	22
GGTGTAC	40	1.4778561E-7	43.848408	23
TCGATAA	65	1.8189894E-12	42.402855	23
CGCCTGA	60	4.1836756E-11	42.317883	22
ATCCGCA	90	0.0	41.76039	23
GCCTGAC	60	4.9112714E-11	41.76039	23
ATCGGTA	60	4.9112714E-11	41.76039	24
ACATCAG	105	0.0	40.567234	23
CTCGCAA	25	0.0012395857	40.089973	25
GCTCGCA	25	0.0012395857	40.089973	24
CTCGATA	70	5.456968E-12	39.899715	22
AACATCA	105	0.0	38.690636	22
TCCAGTA	40	6.7878173E-6	38.086094	21
TCCGCAG	100	0.0	37.58435	24
CCGCAGA	100	0.0	37.58435	25
TTCAACA	110	0.0	36.931973	19
>>END_MODULE