FastQCFastQC Report
Thu 26 May 2016
SRR2099062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442932
Sequences flagged as poor quality0
Sequence length43
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTAGCTAGATAGTCGTATTACCGGCCGACGAGCTAGATCA17580.39690065292189325No Hit
GATCTAGCTAGATAGTCGTATTACCNGCCGACGAGCTAGATCA12210.2756630814662296No Hit
GATCTAGCTAGACCGACGAGCTAGATCGTCTAGATCGTCGGAC10050.22689713093657715No Hit
GATCTAGCTAGACCGACGAGCTAGANCGTCTAGATCGTCGGAC7300.164810851327066No Hit
GACCTAGAGCCTCGGTTTCCTGGACTATTTCCAGTTACAAGTG5430.12259218119259842No Hit
GATCTAGCAATCAATGATTTACGGCACGCTGGCCACTTCGGCC5190.11717374224485926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG556.89397E-1042.38218322
TCGGATG659.276846E-1139.34279325
TAGCGCT3700.035.695455
ATAGCCG301.061542E-535.695454
TAACGCC200.002195259835.695455
TAACGAA200.002195259835.695455
TAGCCGT3800.035.695455
TAGCCAC3200.035.695455
CTAACAA2100.035.695454
TAACCCG301.061542E-535.695455
TACCTCG200.002195259835.695455
TAGTGCC200.002195259835.695455
TAACTCA200.002195259835.695455
TAGAGCC1700.035.6954465
TCGCAGG502.6375346E-1035.6954465
TAGCGTG3050.035.6954465
TAGCGGT4000.035.6954465
ATAGCAC357.443887E-735.6954464
TAACGTG251.5220304E-435.6954465
TAGCATC3000.035.6954465
Produced by FastQC (version 0.11.5)