FastQCFastQC Report
Thu 26 May 2016
SRR2099061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458574
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTACGTGATCGTATTACCGGCCGACGATACGTGCATCA22080.48149262714414687No Hit
TGCACGTACGTGATCGTATTACCGGNCGACGATACGTGCATCA14000.3052942382254554No Hit
TGCACGTACGTGACGACTCACTATAGTGAGTCGTATTACCGGC12540.2734564105248008No Hit
TGCACGTAAAAAAGTGAGTCGTATTACCGGCCGACGATCAAAA9040.19713285096843694No Hit
TGCACGTACGTGACGACTCACTATANTGAGTCGTATTACCGGC8700.18971856232581874No Hit
TGCACGTAAAAAACCGGCCGACGATCAAAAATACGTGCATTTT7930.1729273792234187No Hit
TGCACGGCTACAAGCCGAGGATGCCTGCAAGGTCACCCTGACC7010.1528651864257459No Hit
TGCACGTAGAGGGAATCGAAAAGCATTGAGTCGAATCAAATCG6740.14697736897425498No Hit
TGCACGTATGTGAACGACTCACTATAGCGAGTCGTATTACCGG6620.14436056121803678No Hit
TGCACGTAAAAAAGTGAGTCGTATTNCCGGCCGACGATCAAAA6400.13956308033163678No Hit
TGCACGTAAAAAACGGCCGACGATCAAAAATACGTGCATTTTT6160.13432946481920038No Hit
TGCACGTAAAAAACCGGCCGACGATNAAAAATACGTGCATTTT5560.12124542603810945No Hit
TGCACGTCCCCCTCCTAATCATCCAAATATGGATTGTAGCTCA4950.10794331994400032No Hit
TGCACGGCTACAAGCCGAGGATGCCNGCAAGGTCACCCTGACC4880.10641684875287304No Hit
TGCACCTATTTACGCCCAGGGCGCGATCCTGGAGACCCGGGAT4670.1018374351794912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG600.047.93924
GAGCCCA352.012428E-645.46492822
CCCCCAT352.2184486E-644.82607724
CCCCATA352.2184486E-644.82607725
CGACCGT304.3121265E-544.2095123
TCCCCCA304.3121265E-544.2095123
CTCGACC304.31645E-544.20201521
GCGCTCG304.31645E-544.20201521
TGAACTT304.6926485E-543.5809125
TGTAGGT304.6926485E-543.5809125
TCGGCGT703.6379788E-1241.0905725
AGCCCAA405.035192E-639.7885623
AACCCCG1850.039.57617626
TCCCGGC601.4733814E-939.22281625
CGGCGTA757.2759576E-1238.35119626
ATCGGAT552.9940566E-838.03424525
CGCTCGC351.07105545E-437.8874422
TAATGAA351.07105545E-437.8874422
TAACCCC1950.037.54662725
ATAACCC1950.037.54662724
Produced by FastQC (version 0.11.5)