FastQCFastQC Report
Thu 26 May 2016
SRR2099060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407661
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCACG119200.035.282474
CTAACTA4750.035.1439223
TGCAACG30000.034.9580735
TCTGCAA437400.034.9049033
TGCACGA32250.034.897245
CTGCATA108300.034.8645324
TCTGCAG642650.034.8139273
CTGCAAT114550.034.6750074
CTGCAAC83900.034.4385224
CTGCAGG206200.034.408644
TGCACGG35300.034.256775
CTGCAAG136700.034.118874
CTGCACT98700.034.0993584
CTGCAGT177500.034.093924
TGCAGCG41800.034.0927435
TGCACGT33050.033.9985585
TGCAACC16700.033.9626925
GTCTGCC126900.033.9120062
CTGCATG117100.033.874224
CTGCAGC135100.033.6252144
Produced by FastQC (version 0.11.5)