##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099060_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2407661 Sequences flagged as poor quality 0 Sequence length 43 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.917389948169614 28.0 23.0 31.0 13.0 35.0 2 27.779272497249405 30.0 26.0 31.0 17.0 35.0 3 27.54626668787674 30.0 25.0 31.0 17.0 35.0 4 29.143496945790957 30.0 28.0 32.0 19.0 37.0 5 28.169601534435287 30.0 27.0 32.0 13.0 39.0 6 28.394896956008342 30.0 27.0 31.0 17.0 39.0 7 29.311938433193045 30.0 27.0 32.0 19.0 39.0 8 24.721359443875198 27.0 19.0 31.0 11.0 34.0 9 25.88084202884044 27.0 26.0 28.0 20.0 31.0 10 26.025044223418497 27.0 26.0 27.0 20.0 31.0 11 25.551710560581412 27.0 26.0 27.0 18.0 31.0 12 27.99109882994325 30.0 26.0 31.0 18.0 35.0 13 27.617117609165078 30.0 26.0 31.0 17.0 35.0 14 31.014718849539033 31.0 31.0 33.0 30.0 34.0 15 30.95674307969436 31.0 31.0 34.0 28.0 34.0 16 31.148759729878915 31.0 31.0 34.0 30.0 34.0 17 35.073639519849344 35.0 35.0 37.0 33.0 37.0 18 35.11052220391492 35.0 35.0 37.0 33.0 37.0 19 34.56763348328523 35.0 35.0 37.0 32.0 37.0 20 34.46732658792081 35.0 35.0 37.0 31.0 37.0 21 34.42073655718143 35.0 35.0 37.0 31.0 37.0 22 35.81605716087107 37.0 35.0 39.0 31.0 39.0 23 35.73029384120107 37.0 35.0 39.0 32.0 39.0 24 36.07538104409217 37.0 35.0 39.0 32.0 39.0 25 36.27540546613498 37.0 35.0 39.0 32.0 39.0 26 22.516738444490315 32.0 2.0 37.0 2.0 39.0 27 29.40969264360722 34.0 17.0 38.0 17.0 40.0 28 34.07919055049693 36.0 32.0 39.0 27.0 40.0 29 36.33330647462412 37.0 34.0 39.0 32.0 40.0 30 37.228465718388094 38.0 36.0 40.0 33.0 40.0 31 37.71163049947646 39.0 37.0 40.0 33.0 41.0 32 38.13322930429159 39.0 37.0 40.0 34.0 41.0 33 38.22586153117071 39.0 38.0 40.0 34.0 41.0 34 38.231806720298245 39.0 38.0 40.0 34.0 41.0 35 38.204293710784036 40.0 38.0 40.0 34.0 41.0 36 38.15490012921254 39.0 37.0 40.0 34.0 41.0 37 38.13363883038351 39.0 37.0 40.0 34.0 41.0 38 38.0933815017978 39.0 37.0 40.0 34.0 41.0 39 37.97218545301851 39.0 37.0 40.0 34.0 41.0 40 37.87949590910016 39.0 37.0 40.0 34.0 41.0 41 37.801969214104474 39.0 37.0 40.0 34.0 41.0 42 37.708995161694276 39.0 37.0 40.0 33.0 41.0 43 37.62073439740894 39.0 37.0 40.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.211639737300143 1101 2 5.355983269080443 1101 3 5.174406438428214 1101 4 7.95329632331169 1101 5 9.003603440646842 1101 6 7.424221468125623 1101 7 6.66295465366327 1101 8 5.862885437215272 1101 9 6.035771990181125 1101 10 5.689973393661322 1101 11 3.5766054696276015 1101 12 8.027197082622962 1101 13 6.773332928065489 1101 14 0.15235051972050329 1101 15 0.9846426265385979 1101 16 1.566000356515591 1101 17 0.8453313015509991 1101 18 0.28484036796159984 1101 19 0.01839544439707197 1101 20 0.011238565886152685 1101 21 -0.06602967553692451 1101 22 -0.16818192361463957 1101 23 0.8219976456219555 1101 24 0.3384459379636553 1101 25 0.16364556917566375 1101 26 11.908842098259314 1101 27 6.85515381118649 1101 28 2.9199177556842173 1101 29 1.2343586841259295 1101 30 0.6043711038383464 1101 31 0.25928541069220046 1101 32 0.05931527364963074 1101 33 -0.021897923315364665 1101 34 0.10122370495003707 1101 35 0.15092860418015874 1101 36 0.05995378058553058 1101 37 -0.04300457469074104 1101 38 -0.0252838267731903 1101 39 -0.02263529306949863 1101 40 -0.09439245422203868 1101 41 -0.21038726928634333 1101 42 -0.18732769496053692 1101 43 -0.18130772085871882 1102 1 3.33033809835408 1102 2 2.2634014732982948 1102 3 1.496854586803611 1102 4 4.345687223599995 1102 5 5.37437105118957 1102 6 3.4813995915048324 1102 7 3.4806808360379797 1102 8 2.0815127737995276 1102 9 4.452775852904782 1102 10 3.6926556690443917 1102 11 3.9030170722006687 1102 12 5.711958616529074 1102 13 4.248672380777535 1102 14 -0.11156500756316845 1102 15 1.3137594394310597 1102 16 1.6867979091130216 1102 17 0.9417555963776465 1102 18 0.39178560220285874 1102 19 0.16776356306919382 1102 20 0.07725004167220106 1102 21 -0.07805669446203467 1102 22 -0.10453787719092844 1102 23 0.7183348362894577 1102 24 0.4188989842657307 1102 25 0.2557423293729144 1102 26 11.96376732676871 1102 27 6.8222299063425496 1102 28 2.9019289804383277 1102 29 1.2527738024546409 1102 30 0.617208228827252 1102 31 0.30211528276451105 1102 32 0.17071949316388668 1102 33 0.05673702927525426 1102 34 0.09101790128028853 1102 35 0.11587092781538644 1102 36 0.08684214291378112 1102 37 -0.027633354194641413 1102 38 -0.06386043639987093 1102 39 -0.03706754568352011 1102 40 -0.04474893406820257 1102 41 -0.0699981781850667 1102 42 -0.01456098788980853 1102 43 -0.011845326887822694 1103 1 -0.5984639607542768 1103 2 -0.515972449750155 1103 3 -0.14974543991581157 1103 4 1.9843398147666313 1103 5 2.003244943925168 1103 6 -1.7007988429567256 1103 7 -0.31438790570132014 1103 8 -2.1088000954149777 1103 9 2.3249379820620923 1103 10 1.4561720957661421 1103 11 -1.4233997512530614 1103 12 1.8930397240420191 1103 13 1.5268350109887159 1103 14 -0.6239116098958455 1103 15 1.2875650651241912 1103 16 1.6034545380837564 1103 17 0.8860197174238493 1103 18 0.3310523518067967 1103 19 0.10848669110117726 1103 20 -0.017673005953923848 1103 21 0.007789953950450013 1103 22 -0.07970370399795712 1103 23 0.8193493876604947 1103 24 0.46338801459931744 1103 25 0.3010341690564289 1103 26 11.939847445780089 1103 27 6.980806445179859 1103 28 2.918080905842089 1103 29 1.2856982141420303 1103 30 0.5199961251114615 1103 31 0.29183979575656593 1103 32 0.16647623469449258 1103 33 0.026151540478252855 1103 34 0.2036662388347068 1103 35 0.14632420375414057 1103 36 0.08163376884304796 1103 37 0.04085480831481192 1103 38 -0.03728882554138835 1103 39 -0.04757252576307991 1103 40 -0.09416096892481107 1103 41 -0.04894950401229892 1103 42 0.08983991426825355 1103 43 -0.07103565502881537 1104 1 0.8337009447453099 1104 2 -0.7890096932036208 1104 3 -0.7937334742128463 1104 4 1.1578545001649516 1104 5 0.8641330194466406 1104 6 -0.7894810699181996 1104 7 -0.40753648290290556 1104 8 0.013734957198384024 1104 9 2.161550675255423 1104 10 1.1109294050545842 1104 11 0.7019612717252279 1104 12 2.7843275739571602 1104 13 1.2663173487935104 1104 14 0.27652692676234736 1104 15 1.9369004356713262 1104 16 1.808340241817632 1104 17 0.919401689948252 1104 18 0.3063568390457334 1104 19 -0.013394309397675386 1104 20 -0.1014239557503771 1104 21 -0.14160256712122532 1104 22 -0.22920484489694815 1104 23 0.7038533104916311 1104 24 0.3859599613235076 1104 25 0.12467694792374573 1104 26 11.842978764899819 1104 27 6.814453027316013 1104 28 2.8263995070790955 1104 29 1.1043024492443791 1104 30 0.39637345100307186 1104 31 0.11777897812586247 1104 32 0.06438443450336706 1104 33 -0.004263002758868595 1104 34 -0.005612145908315824 1104 35 -0.01582663821342578 1104 36 -0.047832164346388595 1104 37 -0.13749626501263634 1104 38 -0.1779807362911896 1104 39 -0.06563556791291347 1104 40 -0.22663864959763913 1104 41 -0.2076169987607841 1104 42 -0.15636654030429042 1104 43 -0.16521824958983444 1105 1 0.8498957012750701 1105 2 -1.8747439488931121 1105 3 -1.3540448376866046 1105 4 -0.30010464483142485 1105 5 -1.730472319383253 1105 6 -2.244287365840073 1105 7 -3.4240510847818086 1105 8 -1.7942712668487424 1105 9 1.6626483216851966 1105 10 0.6641811638285198 1105 11 1.0393433624610857 1105 12 2.989100690617626 1105 13 0.5380961886717586 1105 14 0.1811002305374636 1105 15 1.7995742717892576 1105 16 1.6519884340530844 1105 17 0.838997962510831 1105 18 0.21665322406280296 1105 19 -0.05474761637329095 1105 20 -0.11097767147264648 1105 21 -0.09990893377090515 1105 22 -0.15997563327442776 1105 23 0.7413051186384862 1105 24 0.2608712102127484 1105 25 0.05225747522003843 1105 26 11.846787621222973 1105 27 6.739315722520665 1105 28 2.843056042465122 1105 29 1.0706242017866145 1105 30 0.44878685313080524 1105 31 0.14573277893997272 1105 32 0.13323420099236216 1105 33 -0.09059339283228951 1105 34 -0.04759613193097323 1105 35 -0.08530988282295482 1105 36 -0.09766249953066364 1105 37 -0.09555747378637847 1105 38 -0.21833170187596806 1105 39 -0.16678131744472324 1105 40 -0.2687497545189288 1105 41 -0.25573266248528626 1105 42 -0.17192461001951642 1105 43 -0.12306270084308579 1106 1 -4.29545213665827 1106 2 -1.6105358756605987 1106 3 -7.842943624665583 1106 4 -0.3275957928609934 1106 5 0.813582142871141 1106 6 -1.4901412061689179 1106 7 -3.260836724976965 1106 8 -1.1975106906525603 1106 9 -0.5510638146807736 1106 10 0.3552214480846878 1106 11 0.9417081314715929 1106 12 2.8924073672564 1106 13 -4.453523762550418 1106 14 0.16720791288447145 1106 15 1.8157878840702004 1106 16 1.7061097690455327 1106 17 0.8247544071689177 1106 18 0.1761355346475284 1106 19 -0.04147927513022864 1106 20 -0.034996301595754176 1106 21 -0.06746996715838804 1106 22 -0.10236758113281041 1106 23 0.7958562382798107 1106 24 0.3741502120859721 1106 25 0.3123320746753322 1106 26 11.89416278018858 1106 27 6.827130247904421 1106 28 2.7389915268397473 1106 29 1.0961780234433647 1106 30 0.3418093491081109 1106 31 0.10904330198017931 1106 32 -0.12185421824862885 1106 33 -0.09801248114492012 1106 34 -0.09452250140281393 1106 35 -0.14298597166938976 1106 36 -0.21555947074708826 1106 37 -0.2741046800933802 1106 38 -0.2763119162327001 1106 39 -0.20905335561003113 1106 40 -0.25533417371999434 1106 41 -0.2518053784772647 1106 42 -0.13055621383517746 1106 43 -0.2008576256408503 1107 1 1.7086977766266642 1107 2 1.3238330962342992 1107 3 -3.326105130628399 1107 4 -24.796244517674296 1107 5 -23.92109448105446 1107 6 -10.414047097628305 1107 7 -5.793956811055235 1107 8 -2.4793948825775622 1107 9 1.2061196084401402 1107 10 1.7561610407000714 1107 11 2.2354527031735785 1107 12 4.1690433543381396 1107 13 2.6068091511416043 1107 14 0.2356163411045351 1107 15 1.793702112563519 1107 16 1.6338942748106788 1107 17 0.728973333923193 1107 18 0.19243185225988668 1107 19 0.085701043817771 1107 20 0.1478496556082547 1107 21 0.20329821699713335 1107 22 -0.0067983987409334645 1107 23 0.9931949678418306 1107 24 0.6827766596953424 1107 25 0.4931480950177587 1107 26 12.248343962830681 1107 27 7.148451205837681 1107 28 3.16366750385189 1107 29 1.3593696201556043 1107 30 0.77900610842579 1107 31 0.36910936808708783 1107 32 0.13582445056422898 1107 33 -0.028985241704489795 1107 34 -0.27183597155169537 1107 35 -0.10892831071622311 1107 36 -0.31112185239803836 1107 37 -9.674213011834354E-4 1107 38 -0.23496301793306174 1107 39 -0.03542318923187082 1107 40 -0.11544608019305258 1107 41 -0.21402383412567616 1107 42 -0.2000142605421047 1107 43 -0.21383641817660504 1113 1 -5.141651434262094 1113 2 -0.5695239944777448 1113 3 1.3743731528194552 1113 4 1.6518686898728738 1113 5 -23.92109448105446 1113 6 -10.956405851607727 1113 7 -2.0759220254361033 1113 8 1.0287841030551164 1113 9 -20.99970408521762 1113 10 -21.681413749886286 1113 11 -11.61194063322037 1113 12 -22.21707551283378 1113 13 -11.444646772272836 1113 14 0.029235716287981717 1113 15 1.6457239984528407 1113 16 1.1303250234315207 1113 17 0.7248264546192047 1113 18 -0.2730045484596033 1113 19 -0.5143019666209199 1113 20 0.3074198402234103 1113 21 0.49962322395877123 1113 22 0.25795275087034497 1113 23 1.2349708256287073 1113 24 0.5208000056474305 1113 25 0.8758162468341766 1113 26 12.589437956252873 1113 27 7.4088635606780855 1113 28 3.8403783737934134 1113 29 1.7977440585206352 1113 30 1.1369905444577313 1113 31 1.0051680264850802 1113 32 0.573903489632194 1113 33 0.28442588638215227 1113 34 0.573933609470501 1113 35 0.03189060387010301 1113 36 0.3133917736001095 1113 37 0.27604938447283445 1113 38 0.6717660651625721 1113 39 0.5477213645082202 1113 40 0.8109355230160702 1113 41 0.5174281004574937 1113 42 0.5241749906865323 1113 43 0.6656199718558327 1114 1 -3.233449198438329 1114 2 -0.3907719078775109 1114 3 -7.654396638267599 1114 4 -1.3364589948860939 1114 5 0.35906637685438625 1114 6 -0.9507277697624161 1114 7 0.8000453983081428 1114 8 0.9849654981686804 1114 9 -5.081473522214939 1114 10 0.622875794349639 1114 11 1.3580376155529628 1114 12 -22.21707551283378 1114 13 -8.856174922406886 1114 14 -0.29670388611982546 1114 15 -27.54295524683018 1114 16 -13.795379362222786 1114 17 -6.3745715928288185 1114 18 -2.4915537922250977 1114 19 -1.3838704829849249 1114 20 -0.7719341989287969 1114 21 -0.34015117029485253 1114 22 0.07196711679679879 1114 23 0.9155565912212964 1114 24 0.5596992924095332 1114 25 0.4227837211626735 1114 26 12.096266829741673 1114 27 6.8436478188600915 1114 28 2.8322469559169434 1114 29 1.0743007887548401 1114 30 0.37803713255019744 1114 31 -0.05979479409258914 1114 32 -0.14803489875366438 1114 33 -0.34609411968796167 1114 34 -0.34064224160947276 1114 35 -0.26679673489260836 1114 36 -0.2907991313520881 1114 37 -0.3248156061690821 1114 38 -0.313210413162075 1114 39 -0.16103982725666555 1114 40 -0.49475773410365775 1114 41 -0.516754205172532 1114 42 -0.5927125605300247 1114 43 -0.4649253263383102 1115 1 -2.559698680772293 1115 2 0.04924422150630292 1115 3 0.5199859768722241 1115 4 -2.8490900461295823 1115 5 2.794352673417084 1115 6 1.6625157621367102 1115 7 1.6944415272560285 1115 8 3.2478744543359888 1115 9 -20.99970408521762 1115 10 -21.681413749886286 1115 11 -8.623598863010214 1115 12 -22.21707551283378 1115 13 -8.517817503980154 1115 14 0.29231287536146056 1115 15 1.6806219889421783 1115 16 -27.63854290109678 1115 17 -15.203153296876422 1115 18 -4.741788104219694 1115 19 -1.4916757969630012 1115 20 -0.9890596811755827 1115 21 -0.006512686456936478 1115 22 0.3429734595680003 1115 23 1.1743787102735865 1115 24 0.9340535721453591 1115 25 0.5885022416186558 1115 26 12.464648878173413 1115 27 7.474786248206847 1115 28 3.4895193463352143 1115 29 1.75667180817549 1115 30 0.928561337524151 1115 31 0.30118735460289514 1115 32 0.04215015323090654 1115 33 0.24427402259936315 1115 34 0.030943887120443492 1115 35 0.20373444495002246 1115 36 -0.04199248778967046 1115 37 0.14765545902414345 1115 38 0.22975041859101708 1115 39 0.11375910035727799 1115 40 0.3895902246574323 1115 41 -0.06184019222543213 1115 42 0.2805409977428255 1115 43 0.37075112694097356 1201 1 7.840158143042416 1201 2 6.331025484128521 1201 3 6.18426139693166 1201 4 8.957863350100688 1201 5 9.735487772956752 1201 6 8.27269463021188 1201 7 7.4751650777739584 1201 8 6.873021598906217 1201 9 6.825445731136526 1201 10 6.337144146885414 1201 11 6.304709316263342 1201 12 9.349423617264812 1201 13 8.010408321483208 1201 14 0.24628432499283193 1201 15 1.9463159690986842 1201 16 1.8298506809639186 1201 17 1.0475197569254462 1201 18 0.4682864071188888 1201 19 0.24133597931753314 1201 20 0.16005179350769794 1201 21 0.0344630490301725 1201 22 -0.026836010805844523 1201 23 0.9831316315549756 1201 24 0.5030445336575298 1201 25 0.23185759359475355 1201 26 -4.769202152585105 1201 27 -2.940538632859475 1201 28 -1.373284549440065 1201 29 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-0.09251650916016274 2215 35 -3.9388375841298284E-4 2215 36 0.03672811977568813 2215 37 0.22221365969597429 2215 38 -0.050299238192977214 2215 39 0.17064532790218578 2215 40 0.32384366898828176 2215 41 0.06682929806228088 2215 42 0.2837784253266804 2215 43 0.17146676882792633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 4.0 24 66.0 25 715.0 26 5589.0 27 23803.0 28 63976.0 29 130316.0 30 220158.0 31 313505.0 32 404254.0 33 441491.0 34 365255.0 35 257966.0 36 111434.0 37 65947.0 38 3182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 2.296350375322822 94.20571522550468 0.6758023117531581 2.8221320874193494 2 97.68463889612275 1.289177578718581 0.3466022389383214 0.6795812862203493 3 1.1851336213860673 1.646203514531323 96.29171216379714 0.8769507002854637 4 0.3826460025996947 2.163567859256565 0.3694236357622357 97.08436250238151 5 0.472640922643358 1.2330044315743964 97.92068167192039 0.3736729738618578 6 96.66652268033872 1.815141491487916 0.4107348453005355 1.1076009828728262 7 0.7620032734207999 1.9065317726771631 0.3220825668427769 97.00938238705926 8 1.8063597019512723 91.12690330029987 1.08740436772634 5.979332630022512 9 33.89139235851853 23.514271027581522 22.050877339993686 20.543459273906258 10 30.804974955372604 24.236754988943062 25.59039711720353 19.367872938480804 11 29.25123450990218 22.503095409606733 26.23650390971635 22.00916617077474 12 30.806537681750967 23.918736492047454 26.06817375827205 19.20655206792953 13 27.295557488170918 22.969157003004913 27.64224903082615 22.093036477998016 14 30.20855924689662 29.121390472534316 21.218258884687067 19.451791395881994 15 28.12530900227676 27.0691626888382 23.676635818938955 21.128892489946082 16 20.710950976654342 26.76894779272086 32.688286758570285 19.831814472054518 17 22.52256443078988 29.877503518975466 27.266172438727875 20.333759611506768 18 21.24791568179949 30.642655431772692 26.23445225322379 21.874976633204028 19 22.831951840396137 30.40228670066093 24.72420328277112 22.04155817617181 20 22.774717869334594 28.623630984594595 26.232056755498384 22.369594390572427 21 22.067018571136053 27.84910334137572 27.52364224033201 22.560235847156225 22 21.871434558270455 26.713976760017292 28.301700280895027 23.112888400817226 23 22.300809522693093 27.25591232906076 28.29184683040215 22.151431317843997 24 21.936269267143505 28.735731483792776 28.261952160208605 21.066047088855118 25 21.025094479663043 28.08900422443193 29.55669423560875 21.32920706029628 26 18.07390184767373 29.96134258934504 30.567158163779375 21.397597399201853 27 20.77886908186054 28.66695270306473 30.018080103205698 20.536098111869027 28 20.854219908483262 28.982404812665646 30.644151738190857 19.51922354066024 29 20.833307373990234 29.054693636139728 30.639418078751095 19.472580911118946 30 21.212890224259798 28.391911794634943 30.869094977932875 19.52610300317238 31 20.811983082335928 28.44162861798235 30.686213715302944 20.06017458437878 32 20.737138658640067 29.46540231369782 30.304473927184933 19.492985100477185 33 20.80056120857546 29.33510988465569 30.395558178663855 19.468770728104996 34 20.045596120051783 29.695002743326405 30.90206636233257 19.357334774289235 35 20.070807310497614 29.58780326632362 30.807700918027912 19.533688505150852 36 20.50126658196482 29.36904323324588 30.60493150821482 19.524758676574486 37 20.42687903321938 29.95778890798995 30.504958962245933 19.110373096544738 38 19.89972840860902 30.04231077381741 30.58320918102673 19.474751636546838 39 20.112299862812915 30.038489637868455 30.630184232747055 19.219026266571582 40 20.01315799857206 30.1569448522861 30.87639829693632 18.953498852205524 41 20.06212668643966 30.123219174127918 30.66652655834854 19.14812758108388 42 20.326823419077687 29.576381392563157 31.400807671844166 18.695987516514993 43 20.259247460502124 30.333298583147712 30.504792825900324 18.902661130449843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 5.0 7 10.0 8 78.5 9 147.0 10 461.5 11 776.0 12 776.0 13 1624.0 14 2472.0 15 4157.5 16 5843.0 17 9459.5 18 13076.0 19 13076.0 20 18716.5 21 24357.0 22 32332.5 23 40308.0 24 47394.0 25 54480.0 26 54480.0 27 61664.5 28 68849.0 29 79204.0 30 89559.0 31 101644.0 32 113729.0 33 113729.0 34 125425.0 35 137121.0 36 144828.5 37 152536.0 38 159994.0 39 167452.0 40 167452.0 41 174850.5 42 182249.0 43 188733.5 44 195218.0 45 200693.0 46 206168.0 47 206168.0 48 207914.0 49 209660.0 50 203894.0 51 198128.0 52 183899.0 53 169670.0 54 169670.0 55 153044.5 56 136419.0 57 116327.5 58 96236.0 59 79437.5 60 62639.0 61 62639.0 62 50041.0 63 37443.0 64 28935.5 65 20428.0 66 16484.5 67 12541.0 68 12541.0 69 9206.0 70 5871.0 71 4118.5 72 2366.0 73 1651.5 74 937.0 75 937.0 76 754.5 77 572.0 78 442.0 79 312.0 80 188.5 81 65.0 82 65.0 83 42.0 84 19.0 85 12.0 86 5.0 87 2.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.49799369595636594 2 8.306817280339716E-5 3 0.0 4 1.6804691358127244 5 3.643286990984196 6 0.0010383521600424645 7 0.4984921049931863 8 4.153408640169858E-5 9 0.37339143675127023 10 0.23047264544302543 11 0.5393616460124577 12 0.6843571416407875 13 1.37959621391882 14 0.031856644270102805 15 0.4499802920760024 16 0.1021738525481785 17 0.0 18 0.01657210047427773 19 0.0 20 0.0 21 0.0 22 0.0 23 0.793010311667631 24 0.0 25 0.0 26 34.8689869545588 27 0.00174443162887134 28 5.814772096237801E-4 29 0.002076704320084929 30 2.9073860481189004E-4 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 2407661.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.18515315744714 #Duplication Level Percentage of deduplicated Percentage of total 1 67.8784589838003 31.34977024258311 2 10.61877178656668 9.808592026131215 3 6.689055138873 9.26805108202375 4 4.883373532507299 9.021574180954932 5 3.322316910698013 7.672085767908218 6 2.1972431209319536 6.088800603863364 7 1.3833304487978209 4.472253005156122 8 0.8531721651317272 3.152310969302368 9 0.5317209772063151 2.2101853292371008 >10 1.5321595585837724 11.436997828370046 >50 0.07425954916101087 2.3568981316184616 >100 0.034999687163037634 2.701149549742853 >500 8.277386029218303E-4 0.2527908108187002 >1k 3.1040197609568504E-4 0.20854047228975497 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 4.153408640169858E-5 0.0 0.0 0.0 0.0 11 4.153408640169858E-5 0.0 4.1534086401698577E-4 0.0 0.0 12 4.153408640169858E-5 0.0 8.306817280339715E-4 0.0 0.0 13 4.153408640169858E-5 0.0 0.0028243178753155035 0.0 0.0 14 4.153408640169858E-5 0.0 0.007683805984314237 0.0 0.0 15 4.153408640169858E-5 0.0 0.020933179546456085 0.0 0.0 16 4.153408640169858E-5 0.0 0.02890772413558221 0.0 0.0 17 4.153408640169858E-5 0.0 0.032894996430145276 0.0 0.0 18 4.153408640169858E-5 0.0 0.036093121083076066 0.0 0.0 19 4.153408640169858E-5 0.0 0.04203249543851896 0.0 0.0 20 4.153408640169858E-5 0.0 0.0526236874709521 0.0 0.0 21 4.153408640169858E-5 0.0 0.08589249067871266 0.0 0.0 22 4.153408640169858E-5 0.0 0.1019246480297683 0.0 0.0 23 4.153408640169858E-5 0.0 0.12742657708041125 4.153408640169858E-5 0.0 24 4.153408640169858E-5 0.0 0.15085180181096924 4.153408640169858E-5 0.0 25 4.153408640169858E-5 0.0 0.1629382209538635 4.153408640169858E-5 0.0 26 4.153408640169858E-5 0.0 0.17909498056412426 4.153408640169858E-5 0.0 27 4.153408640169858E-5 0.0 0.2187600330777464 4.153408640169858E-5 0.0 28 4.153408640169858E-5 0.0 0.23969321262420248 4.153408640169858E-5 0.0 29 4.153408640169858E-5 0.0 0.2689332094509983 4.153408640169858E-5 0.0 30 4.153408640169858E-5 0.0 0.321473828749147 4.153408640169858E-5 0.0 31 4.153408640169858E-5 0.0 0.3607650744851538 4.153408640169858E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCACG 11920 0.0 35.28247 4 CTAACTA 475 0.0 35.14392 23 TGCAACG 3000 0.0 34.958073 5 TCTGCAA 43740 0.0 34.904903 3 TGCACGA 3225 0.0 34.89724 5 CTGCATA 10830 0.0 34.864532 4 TCTGCAG 64265 0.0 34.813927 3 CTGCAAT 11455 0.0 34.675007 4 CTGCAAC 8390 0.0 34.438522 4 CTGCAGG 20620 0.0 34.40864 4 TGCACGG 3530 0.0 34.25677 5 CTGCAAG 13670 0.0 34.11887 4 CTGCACT 9870 0.0 34.099358 4 CTGCAGT 17750 0.0 34.09392 4 TGCAGCG 4180 0.0 34.092743 5 TGCACGT 3305 0.0 33.998558 5 TGCAACC 1670 0.0 33.962692 5 GTCTGCC 12690 0.0 33.912006 2 CTGCATG 11710 0.0 33.87422 4 CTGCAGC 13510 0.0 33.625214 4 >>END_MODULE