FastQCFastQC Report
Thu 26 May 2016
SRR2099059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349498
Sequences flagged as poor quality0
Sequence length43
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTCGATAAGGGAGTCGTATTACCGGCCGACGATCGAATGC12360.3536500924182685No Hit
GCATTCGATAAGGGAGTCGTATTACNGGCCGACGATCGAATGC8680.24835621376946365No Hit
GCATTCGAGAGGATTCTACAGTGAGTCGTATTACCGGCCGACG8170.23376385558715645No Hit
GCATTCGAGACCTAGGAGATTGCCGCCTGGTCGTGCTCTTTCC7520.2151657520214708No Hit
GCATTCAGAAAACCCGCAGTCGGCGCCGCTGCGGGGCTTGAGG6150.1759666721984103No Hit
GCATTCGAGAGGATTCTACAGTGAGNCGTATTACCGGCCGACG5850.16738293209117078No Hit
GCATTCGAGACCTAGGAGATTGCCGNCTGGTCGTGCTCTTTCC5460.15622406995175936No Hit
GCATTCGAAGTTTGGCCGGGAAGCCCAAAATAAGGGCACCAGC5150.14735420517427855No Hit
GCATTCGACACTAGGTGGCGGTTGCCTGTAGTCCCTGCTACTT4140.11845561347990548No Hit
GCATTCAGAAAACCCGCAGTCGGCGNCGCTGCGGGGCTTGAGG4110.11759723946918152No Hit
GCATTCGACTTTGGCCAGGGCATAGGGCACGGGCTCGCCGTCA3860.11044412271314857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGG352.4706078E-644.12413825
GGCCAAG603.092282E-1143.59264821
GTTGCCT900.043.59264421
TTACCCG305.147161E-542.89846825
GCACCCC557.3123374E-1042.11849626
CGGACCG557.3123374E-1042.11849626
CGGCCAA501.2863893E-841.84893820
TCATGTA250.001003809341.84893821
TTGCCTG950.041.29829422
CAGCCGC750.041.18252624
TCAGCCG900.040.68646623
TCGGACC601.715307E-938.6086225
CGTACTG406.185739E-638.6086224
CAAGATA553.4351615E-837.43866324
AAGATAG553.4351615E-837.43866325
TCGTACT351.16143645E-437.36512423
TGCACCC701.9099389E-1036.77011525
CCGCTGC1150.035.81089426
CTTCGCA601.8189894E-1235.387433
GTTCGCG301.1258151E-535.387433
Produced by FastQC (version 0.11.5)