FastQCFastQC Report
Thu 26 May 2016
SRR2099058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102563
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCG55650.035.352464
GCCATGG243900.035.2515223
GCCATGA177600.035.192683
GCCATGC214500.035.1726763
CATGCGT14950.035.1082655
CATGCGG14600.035.093955
CCATGGG71400.035.080254
ACGGCGC251.7034826E-435.0241859
CTCGCGG301.210733E-535.0241859
TACGGTG301.2111181E-535.0225611
TACCGGT650.035.0225610
CCATGCA53950.034.9773374
CATGTCG16150.034.931545
CCATGAG49250.034.7991144
CCATGTC51600.034.7019924
CCATGTG55600.034.5815244
CCATGGT76800.034.4733244
TAGCCGT14700.034.399721
TAGCCCT43450.034.3406221
ACCCGCG754.0017767E-1034.33959224
Produced by FastQC (version 0.11.5)