FastQCFastQC Report
Thu 26 May 2016
SRR2099057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916797
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAAGCTGCACAGAAGAAGTGCAGCCGCAGATGGACTAGGCT12210.13318106407416255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCCG501.4544639E-841.29282421
GGCTCCG200.002152321935.8436475
AGCTACG11250.035.684345
TAAGCTC162750.035.6323853
TAAGCTA148450.035.4794433
TAAGCTG204550.035.4561123
CGTACGC18700.035.4448471
AAGCTAG38450.035.142484
AGCTAGG12200.035.1091465
AAGCTCG41250.035.1030464
AAGCTAC38200.035.090994
ACGTCGC251.6899657E-435.070889
ATTGCGG251.6899657E-435.070889
TTCGCGA503.2014214E-1035.070888
TCGCGCG406.1383616E-835.070888
TCCGCGG454.4201443E-935.0708778
CGCACGG301.1995071E-535.0708779
TCTATCG301.1995071E-535.0708778
GTACGCT18700.034.9671552
AGCTGCG14100.034.953915
Produced by FastQC (version 0.11.5)