##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099056_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 287476 Sequences flagged as poor quality 0 Sequence length 43 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.540518164994644 29.0 24.0 31.0 15.0 35.0 2 27.990952288191014 30.0 26.0 31.0 18.0 35.0 3 28.89945247603278 30.0 28.0 32.0 21.0 35.0 4 27.79363842546856 30.0 25.0 31.0 16.0 38.0 5 26.489640874368643 30.0 23.0 31.0 8.0 38.0 6 25.692478676480818 28.0 20.0 31.0 10.0 37.0 7 24.17300574656667 27.0 17.0 30.0 10.0 34.0 8 27.796097761204415 29.0 26.0 31.0 17.0 35.0 9 25.698507005802224 27.0 26.0 28.0 20.0 31.0 10 25.98501787975344 27.0 26.0 27.0 20.0 31.0 11 25.435149368990803 27.0 26.0 27.0 18.0 31.0 12 28.002431507325827 30.0 26.0 31.0 18.0 35.0 13 27.63225799718933 30.0 26.0 31.0 17.0 35.0 14 30.58053889716011 31.0 30.0 31.0 28.0 34.0 15 30.494215169266305 31.0 30.0 31.0 28.0 34.0 16 30.68632859786556 31.0 30.0 31.0 28.0 34.0 17 34.54756918838442 35.0 35.0 37.0 32.0 37.0 18 34.55724999652145 35.0 35.0 37.0 32.0 37.0 19 34.13345809737161 35.0 35.0 36.0 30.0 37.0 20 34.02317062989606 35.0 33.0 36.0 30.0 37.0 21 33.89330239741752 35.0 33.0 36.0 30.0 37.0 22 35.121617108906484 37.0 34.0 39.0 30.0 39.0 23 34.704330100599705 37.0 33.0 39.0 28.0 39.0 24 34.84219552240883 37.0 33.0 39.0 28.0 39.0 25 34.85251290542515 37.0 33.0 39.0 27.0 39.0 26 22.590310147629715 30.0 2.0 37.0 2.0 39.0 27 28.69175165926895 32.0 17.0 37.0 17.0 40.0 28 32.5032107028065 34.0 29.0 38.0 25.0 40.0 29 34.41026729187828 36.0 32.0 38.0 27.0 40.0 30 35.363797325689795 37.0 33.0 39.0 27.0 40.0 31 35.83102937288678 37.0 34.0 39.0 30.0 40.0 32 36.360624886947086 38.0 35.0 40.0 30.0 41.0 33 36.60919172383086 38.0 35.0 40.0 31.0 41.0 34 36.76885722634237 38.0 35.0 40.0 31.0 41.0 35 36.85146238294675 38.0 35.0 40.0 31.0 41.0 36 36.93733737772892 38.0 36.0 40.0 32.0 41.0 37 37.0528670219427 38.0 36.0 40.0 32.0 41.0 38 37.09072409522882 38.0 36.0 40.0 32.0 41.0 39 36.98694847569884 38.0 36.0 40.0 32.0 41.0 40 36.89322934784121 38.0 35.0 40.0 32.0 41.0 41 36.823202632567586 38.0 35.0 40.0 31.0 41.0 42 36.72317689128832 38.0 35.0 40.0 31.0 41.0 43 36.59521838344766 38.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.934901832278129 1101 2 5.019765498418497 1101 3 5.044726199228748 1101 4 8.588342572189813 1101 5 9.264862152760475 1101 6 6.714183842058361 1101 7 6.1707233356698055 1101 8 5.457608551968445 1101 9 5.702102582960972 1101 10 5.377771947271189 1101 11 3.137114675685851 1101 12 7.515904552052525 1101 13 6.242650204377995 1101 14 0.02861504944909754 1101 15 0.9843419390727846 1101 16 1.3621381909182304 1101 17 0.7946720541326044 1101 18 0.18911891143637405 1101 19 -0.04743277286381442 1101 20 -0.13751890691570878 1101 21 -0.2316038224153587 1101 22 -0.20500917221192339 1101 23 0.342729892472164 1101 24 -0.12240691406029214 1101 25 -0.2018248664715543 1101 26 10.17465088605432 1101 27 5.347332361682348 1101 28 1.9378723779562748 1101 29 0.3464546418378376 1101 30 -0.2372618520533507 1101 31 -0.20189927303906074 1101 32 -0.4739574183221009 1101 33 -0.7985078257219911 1101 34 -0.3005940630617374 1101 35 -0.3407617091556858 1101 36 -0.31322859221999977 1101 37 -0.4657537475116129 1101 38 -0.23850501871118723 1101 39 -0.2788621879058084 1101 40 -0.4247799555533902 1101 41 -0.4124345228934061 1101 42 -0.4693843607571395 1101 43 -0.3431027669477231 1102 1 3.399128632151701 1102 2 2.473920427028208 1102 3 1.9408704364726255 1102 4 4.612867850811202 1102 5 5.206149239294575 1102 6 2.822902664633908 1102 7 3.3748426722004723 1102 8 2.094422579919911 1102 9 4.636465842041076 1102 10 3.911145774038083 1102 11 4.1085492151188525 1102 12 5.559162532786079 1102 13 4.145098224826263 1102 14 -0.04830616700296275 1102 15 1.339290337429226 1102 16 1.607613049717994 1102 17 0.963482399197126 1102 18 0.31896980948361886 1102 19 0.011862806297642692 1102 20 -0.018716120014687476 1102 21 -0.030155953130361013 1102 22 -0.0877427538194766 1102 23 0.7015084932499676 1102 24 0.1214958467307028 1102 25 0.026502580655652253 1102 26 10.433286293989017 1102 27 5.542039448491288 1102 28 2.1686783530280778 1102 29 0.2883249437521158 1102 30 -0.2775916628244204 1102 31 -0.3359794445406621 1102 32 -0.57200796182277 1102 33 -0.5450235123467948 1102 34 -0.4733110167095873 1102 35 -0.4416964478493597 1102 36 -0.4146388081086343 1102 37 -0.42162506634243613 1102 38 -0.38351072168910605 1102 39 -0.5250945121097956 1102 40 -0.38275656647287803 1102 41 -0.4053232529845161 1102 42 -0.3478826223795366 1102 43 -0.29646037801837366 1103 1 -0.6357867688367911 1103 2 -0.047793920531226775 1103 3 0.10792119587953053 1103 4 2.1636929166505183 1103 5 1.2483939090157072 1103 6 -2.092835998377023 1103 7 -2.017796311889864 1103 8 -0.9740438149716795 1103 9 2.3966779709057526 1103 10 1.4294877055651938 1103 11 -1.052092222162436 1103 12 2.2443661837114384 1103 13 1.8107701466127644 1103 14 -0.4247995469950254 1103 15 1.1848929926476686 1103 16 1.4108180715441776 1103 17 0.769602389095752 1103 18 0.18898243620175492 1103 19 0.1220081939378872 1103 20 -0.035876190724309254 1103 21 -0.09829188877017714 1103 22 -0.12170357491691419 1103 23 0.5710759379629806 1103 24 0.12852856796884282 1103 25 -0.20008062941685978 1103 26 10.319334131212589 1103 27 6.00224219750821 1103 28 2.2460794136724047 1103 29 0.39208193354250653 1103 30 0.034748609191169066 1103 31 -0.27470811440610277 1103 32 -0.2794634977367636 1103 33 -0.4949423405873503 1103 34 -0.11307291288188281 1103 35 -0.15687664915532196 1103 36 -0.01484011407205088 1103 37 -0.1504284142265604 1103 38 -0.26673450321080594 1103 39 -0.276597813094682 1103 40 -0.24963488098638464 1103 41 -0.29902360292499 1103 42 -0.12827008224461167 1103 43 -0.33382175469141373 1104 1 1.1993835404612412 1104 2 -0.34643102853944896 1104 3 -0.7287301286820558 1104 4 1.5607511699175518 1104 5 0.7346471641172307 1104 6 -0.5911208246075468 1104 7 0.547067085705315 1104 8 -0.2871286519406837 1104 9 2.1096339461237292 1104 10 1.254113424819586 1104 11 0.9889854438887582 1104 12 2.763301702126437 1104 13 1.4768004589533632 1104 14 0.11279706203185924 1104 15 1.8110596836747739 1104 16 1.6283259688007945 1104 17 0.8469716406535781 1104 18 0.06946167066750064 1104 19 -0.23519986111471525 1104 20 -0.15107770127039544 1104 21 -0.30062720971647394 1104 22 -0.3929739668415806 1104 23 0.41109198602364216 1104 24 -0.14056579045175965 1104 25 -0.18483348033395686 1104 26 10.161471028925586 1104 27 5.274477561110132 1104 28 1.7798743027528587 1104 29 0.17042779013011966 1104 30 -0.14781448683172016 1104 31 -0.351286894574514 1104 32 -0.5063034317547732 1104 33 -0.6250017303434348 1104 34 -0.43587010775247137 1104 35 -0.483917034786046 1104 36 -0.5474254327811678 1104 37 -0.5005818849925419 1104 38 -0.34384275006457443 1104 39 -0.20855314102099243 1104 40 -0.2576425053066558 1104 41 -0.3119008292951122 1104 42 -0.21533677714949562 1104 43 -0.3614837789544083 1105 1 1.0086466472390043 1105 2 -1.8726198673325172 1105 3 -0.9755958281003387 1105 4 -1.165758114125758 1105 5 -5.2059825020759405 1105 6 -2.859602039159018 1105 7 -2.6842503310174486 1105 8 -1.2929821572831344 1105 9 1.8525645953161458 1105 10 0.8411941307878052 1105 11 1.2043954582390057 1105 12 3.245848776609602 1105 13 0.8320236147575066 1105 14 0.15093208230685562 1105 15 1.7156247199574928 1105 16 1.4631062006438036 1105 17 0.677207627353063 1105 18 0.20340385966913743 1105 19 -0.09772027059837995 1105 20 -0.2683804362502471 1105 21 -0.08948810245600214 1105 22 -0.40760700323479426 1105 23 0.31419800774820317 1105 24 0.05030486394570488 1105 25 0.1766342868576274 1105 26 10.408410878560577 1105 27 5.647815178678208 1105 28 2.2140718011280995 1105 29 0.6795053759735339 1105 30 -0.02918286365439826 1105 31 -0.05637403561840415 1105 32 -0.11047285482246849 1105 33 -0.17975948449330303 1105 34 -0.282478753086167 1105 35 -0.2737680510074938 1105 36 -0.35964364378360614 1105 37 -0.38333003741630733 1105 38 -0.33293976035692197 1105 39 -0.3022652026441861 1105 40 -0.36559431638934825 1105 41 -0.42861327039294395 1105 42 -0.2639451520529903 1105 43 -0.07748554111250172 1106 1 -3.9205026248483854 1106 2 -1.3118941676129552 1106 3 -6.016789596266239 1106 4 0.07487009083877183 1106 5 0.9540763522436606 1106 6 -1.581429204066012 1106 7 -3.2072569228714656 1106 8 -2.449879134562728 1106 9 -0.4095658629645236 1106 10 0.3778908416696858 1106 11 1.0857431498134318 1106 12 3.3549090650510927 1106 13 -3.9141091500337346 1106 14 0.20190405096895248 1106 15 1.755636507843633 1106 16 1.6710549382335422 1106 17 0.7181364872474418 1106 18 0.13893965526633423 1106 19 0.17763432348554886 1106 20 -0.16326079133371962 1106 21 -0.29497287395400207 1106 22 -0.016822619417816043 1106 23 0.6167424038211777 1106 24 0.12488345765514453 1106 25 0.1560597444361278 1106 26 10.687062463541874 1106 27 5.529683111797915 1106 28 2.320090431945971 1106 29 0.7093527717869179 1106 30 -0.003271855811405544 1106 31 -0.12230321467937699 1106 32 -0.4111894902108091 1106 33 0.03446300441033401 1106 34 -0.13077461626157572 1106 35 -0.23462226847484402 1106 36 -0.29128135140147293 1106 37 -0.17475679719450454 1106 38 -0.20206762700485825 1106 39 -0.3277144828171288 1106 40 -0.2086590391186931 1106 41 -0.18379228029562 1106 42 -0.027961884893535682 1106 43 -0.16002202185399028 1107 1 1.7369266513810793 1107 2 0.8409557772776104 1107 3 -4.301784085323078 1107 4 -23.484490639754835 1107 5 -22.364416798441273 1107 6 -9.327138541178279 1107 7 -5.651320849812105 1107 8 -2.307893620470459 1107 9 0.8932604635982209 1107 10 1.4755526272152686 1107 11 1.8803817893977524 1107 12 3.9559036590782775 1107 13 2.4817077816830526 1107 14 0.19721974928417652 1107 15 1.6213925567598118 1107 16 1.504112724408948 1107 17 0.8091326558298135 1107 18 -0.07442569402248722 1107 19 -0.3623987421734043 1107 20 -0.3626125994479281 1107 21 -0.1992530398071466 1107 22 -0.6637942249392594 1107 23 0.837652496719933 1107 24 0.4757993239229279 1107 25 0.5626755004850494 1107 26 10.972975443125147 1107 27 5.361494375116024 1107 28 2.263983782074561 1107 29 0.4747834126308845 1107 30 0.10709134158532407 1107 31 -0.45235622470404024 1107 32 -0.635169965726476 1107 33 -0.44826937369285247 1107 34 -0.1978769098636377 1107 35 -0.4139772256994263 1107 36 -0.8721216390202216 1107 37 -0.4482570333777858 1107 38 -0.6998238858617327 1107 39 -0.7921688994444338 1107 40 -0.35347875254964833 1107 41 -0.6137639383022346 1107 42 -0.6439803596744085 1107 43 -0.43546420943599173 1113 1 -4.358858789231949 1113 2 -0.5306917323009337 1113 3 0.4185224280868809 1113 4 3.015509360245165 1113 5 -22.364416798441273 1113 6 -9.953575322787474 1113 7 -1.262431960923216 1113 8 0.6844435442804979 1113 9 -20.8193832145627 1113 10 -21.616401395773238 1113 11 -10.747970701023705 1113 12 -22.17819595777996 1113 13 -11.363119804523842 1113 14 0.7144611285945217 1113 15 2.2210094149973614 1113 16 2.099898165021976 1113 17 1.2459142650252204 1113 18 0.5213597465905409 1113 19 -2.295348933744286 1113 20 0.7983069407819556 1113 21 0.15898450808557385 1113 22 1.294060181191007 1113 23 1.6958900446126535 1113 24 2.130971737716031 1113 25 2.5856640062321716 1113 26 13.50745820174583 1113 27 7.805938819560474 1113 28 3.5111102188561745 1113 29 2.388576516079155 1113 30 2.82739785499529 1113 31 1.290938794453048 1113 32 1.3437572373386573 1113 33 1.9195467182611736 1113 34 1.3212801782589665 1113 35 1.4940687513120636 1113 36 1.9305603533169418 1113 37 1.5383330049363906 1113 38 1.417034351686162 1113 39 1.9626203725862226 1113 40 0.7901994083698938 1113 41 1.93987590844106 1113 42 2.405828069444368 1113 43 1.8346507330927437 1114 1 -2.619275455898613 1114 2 -0.8501361767453766 1114 3 -5.53633868302423 1114 4 -0.421990639754835 1114 5 0.6772498682253953 1114 6 -1.3598253227874757 1114 7 2.3486791501878947 1114 8 0.8684713220582765 1114 9 -5.173549881229366 1114 10 0.9460986042267621 1114 11 1.4881404100874072 1114 12 -22.17819595777996 1114 13 -8.741592026746066 1114 14 0.05821112859452171 1114 15 -27.167879473891528 1114 16 -13.288990723866913 1114 17 -5.229780179419226 1114 18 -2.905723586742795 1114 19 -0.9550711559665075 1114 20 -1.229470836995823 1114 21 -0.5007377141366476 1114 22 -1.0045509299201 1114 23 0.4806122668348749 1114 24 0.7490272932715882 1114 25 1.339136228454393 1114 26 11.597735979523609 1114 27 6.733022152893803 1114 28 3.21249910774506 1114 29 1.607326516079155 1114 30 0.4905922994397329 1114 31 0.7492721277863765 1114 32 0.8368127928942144 1114 33 0.9334356071500594 1114 34 0.6754468449256379 1114 35 0.6537909735342851 1114 36 1.0902825755391632 1114 37 0.7119441160475048 1114 38 0.4725899072417121 1114 39 0.247342594808444 1114 40 0.34228274170322237 1114 41 0.5475147973299528 1114 42 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0.26611590887250003 1115 37 0.9361107827141666 1115 38 -0.11407675942495388 1115 39 0.531509261475108 1115 40 0.8768660750365598 1115 41 1.3154314639966174 1115 42 1.1124947361110316 1115 43 1.0635396219816329 1201 1 7.686418338012395 1201 2 6.151327523488813 1201 3 5.852298124157663 1201 4 9.661489063601916 1201 5 9.949400531769502 1201 6 7.934402819289026 1201 7 7.219873529578994 1201 8 6.2319324852120594 1201 9 6.584207730011851 1201 10 6.424191890721687 1201 11 6.221844547480067 1201 12 9.141866493430033 1201 13 7.724181782777748 1201 14 0.19377416528460856 1201 15 1.913307099098322 1201 16 1.6548031871401143 1201 17 1.0622036222981777 1201 18 0.4468086146623591 1201 19 0.16275671288595106 1201 20 0.18914742998310174 1201 21 0.12840943652689418 1201 22 0.13220746196124367 1201 23 1.2319295970456494 1201 24 0.5288379880412961 1201 25 -0.12419808067909344 1201 26 -4.233499383474047 1201 27 -2.4844593069032292 1201 28 -1.5676043270714892 1201 29 -0.693781017098047 1201 30 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31 1.4576054611197122 2215 32 1.8311309747123943 2215 33 1.323587122301575 2215 34 1.1091589661377554 2215 35 1.6178061250494409 2215 36 1.9179340906906788 2215 37 0.9752016918050757 2215 38 0.6695596042114147 2215 39 1.1015092614751083 2215 40 1.0250478932183782 2215 41 1.1317951003602502 2215 42 0.5270401906564857 2215 43 0.6780850765270827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 43.0 25 408.0 26 2023.0 27 7357.0 28 16868.0 29 28174.0 30 38186.0 31 43987.0 32 45129.0 33 41026.0 34 30656.0 35 20468.0 36 8044.0 37 4846.0 38 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 2.3069480875557526 94.11859215043886 0.6758432323119427 2.898616529693443 2 1.448816596863738 1.5973507353657348 95.57771779209395 1.3761148756765782 3 1.020606937622619 2.3236722369867397 0.8181552546995227 95.83756557069111 4 94.96912671700495 2.515968633737529 0.7206721544621736 1.7942324947953427 5 93.39262058331704 3.3603347304565627 1.7623297934718876 1.4847148927545044 6 1.198369243827268 2.918176948176183 92.99901904172204 2.884434766274515 7 3.3602850007858476 90.04802402947803 1.3764560012573563 5.215234968478773 8 1.4839499645187773 2.8705004939542778 2.1288733668201867 93.51667617470676 9 30.04604209063904 26.21083018881097 18.968014884266452 24.77511283628354 10 34.712328003234305 14.664510462700925 22.76909564902204 27.85406588504273 11 20.461168610623858 26.409747506866164 23.88900924573529 29.240074636774686 12 34.1701573247783 18.245751670612627 27.357069808492458 20.227021196116613 13 20.128177122603287 27.271638257242113 21.268664604370283 31.33152001578432 14 28.1628491999151 26.487176721513393 20.35928143712575 24.990692641445754 15 24.964521157973476 27.757038093440435 23.846675475206755 23.431765273379334 16 21.955295592228953 26.144418912332014 31.594248311398925 20.30603718404011 17 23.226634571233774 26.53578037818809 27.45411790897327 22.783467141604866 18 21.311355642683942 25.667697595697238 27.548418295105463 25.47252846651336 19 20.69598853469507 28.15574169669816 24.256285742114127 26.89198402649265 20 23.1692384755597 26.65752967204219 23.737981605420973 26.435250246977137 21 21.472749029484202 29.594122639802972 24.702583867870707 24.230544462842115 22 20.845914093698255 29.09773337600356 24.215586692454327 25.840765837843854 23 22.101406080157087 28.86812300571549 25.986535292261298 23.043935621866122 24 22.05714563998386 31.104857448969653 25.50473778680655 21.333259124239937 25 18.20778082344265 30.191737745063936 29.56525066440329 22.035230767090123 26 16.076794065636733 29.90894765376801 30.80781722647561 23.20644105411965 27 20.219082968368983 29.153549401504847 28.530530036073205 22.096837594052964 28 20.038959909557352 30.01826245760501 29.553874249934776 20.38890338290286 29 18.36574251226215 30.74268619334191 29.868508018227992 21.023063276167946 30 18.97493338528006 33.35779931402492 27.729116372263228 19.93815092843179 31 19.68094727907721 33.139114221708944 28.13278325842853 19.04715524078532 32 18.355967106819353 34.32077808234427 27.451335067970888 19.87191974286549 33 18.109337822983484 33.55236611056227 28.203745703989203 20.13455036246504 34 19.420403790229447 32.36026659616803 29.035119453450026 19.1842101601525 35 20.909223726502386 33.30817181260349 29.07233995185685 16.710264509037277 36 20.309173635364346 35.10623495526583 28.30253655957367 16.282054849796157 37 19.126814064478424 34.86934561493829 29.06260000834852 16.941240312234758 38 19.12472693372664 35.57862221541972 28.82466710264509 16.471983748208547 39 19.733125547871822 35.21546146460922 28.468811309465835 16.582601678053123 40 19.04332883440705 35.80611946736423 27.99155407755778 17.15899762067094 41 17.62929775007305 37.1432745690075 27.213402162267457 18.01402551865199 42 17.693650948253072 36.41486593663471 27.1807037804895 18.710779334622714 43 16.25979212177712 36.66358235122236 27.99433691856016 19.082288608440358 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 4.5 7 8.0 8 34.0 9 60.0 10 163.0 11 266.0 12 266.0 13 450.5 14 635.0 15 969.0 16 1303.0 17 1846.5 18 2390.0 19 2390.0 20 2902.5 21 3415.0 22 4208.5 23 5002.0 24 5890.0 25 6778.0 26 6778.0 27 7581.5 28 8385.0 29 9024.0 30 9663.0 31 10424.5 32 11186.0 33 11186.0 34 13342.0 35 15498.0 36 16518.5 37 17539.0 38 18446.5 39 19354.0 40 19354.0 41 20774.5 42 22195.0 43 23491.5 44 24788.0 45 24625.5 46 24463.0 47 24463.0 48 25501.0 49 26539.0 50 25641.0 51 24743.0 52 23081.0 53 21419.0 54 21419.0 55 19126.0 56 16833.0 57 14129.5 58 11426.0 59 8202.0 60 4978.0 61 4978.0 62 3769.0 63 2560.0 64 1921.5 65 1283.0 66 1159.0 67 1035.0 68 1035.0 69 1001.5 70 968.0 71 1081.0 72 1194.0 73 1238.5 74 1283.0 75 1283.0 76 746.5 77 210.0 78 137.0 79 64.0 80 37.5 81 11.0 82 11.0 83 6.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.4059469312220846 2 0.0 3 0.0 4 1.9191167262658448 5 3.8938902725792763 6 6.957102505948323E-4 7 0.4038598004703001 8 0.0 9 0.34750727017211874 10 0.1920160291641737 11 0.44873311163366686 12 0.6790132045805564 13 1.269671207335569 14 0.024002003645521713 15 0.4835186241634084 16 0.08905091207613854 17 0.0 18 0.011479219134814733 19 0.0 20 0.0 21 0.0 22 0.0 23 0.7951968164298933 24 0.0 25 0.0 26 31.347660326427253 27 0.0017392756264870807 28 3.4785512529741616E-4 29 0.0020871307517844966 30 6.957102505948323E-4 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 287476.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.05584763967634 #Duplication Level Percentage of deduplicated Percentage of total 1 80.68243265474905 43.61357286783561 2 8.534760380734921 9.227074135643058 3 3.6022816710815295 5.841731675015454 4 2.0562218825891887 4.446032671944384 5 1.2713742379818584 3.4362606050678473 6 0.8878569786606154 2.879631693858096 7 0.6025804698062956 2.2801098646545594 8 0.43472677007933236 1.8799619238637595 9 0.31131926921530095 1.5145764285597918 >10 1.4139849744537392 14.42031953623983 >50 0.13824987037787964 5.16289286484167 >100 0.06355562761447547 5.110474113109724 >500 6.552126558193348E-4 0.18736161936620999 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCGGTACGGTGCGGCGGAAGTTGCAGTGAGACAAGATCGCAC 529 0.18401536128233315 No Hit ATCGGTACGGTGCGGCGGAAGTTGCNGTGAGACAAGATCGCAC 374 0.13009781686123362 No Hit ATCGGTACCCTCGCAGTCCGACGATCGACGGACTGTAGAACTC 345 0.12001001822760857 Illumina DpnII expression Sequencing Primer (95% over 21bp) ATCGGCGCAATGCGGTCGGACCGGAGGCGCGCCCGTGGTTGCC 328 0.1140964810975525 No Hit ATCGGCGCAATGCGTCGGACCGGAGGCGCGCCCGTGGTTGCCC 323 0.11235720547106542 No Hit ATCGGTACCCATAGTGCTCAAGTTTATTATATTTTCTGCATCA 300 0.10435653758922483 No Hit ATCGGTACGCGTCACTAAACCTAGATCCTTAGGTCATATACGA 296 0.10296511708803517 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0017392756264870807 0.0 0.0 12 0.0 0.0 0.0027828410023793292 0.0 0.0 13 0.0 0.0 0.00904423325773282 0.0 0.0 14 0.0 0.0 0.04000333940920286 0.0 0.0 15 0.0 0.0 0.07687598269072897 0.0 0.0 16 0.0 0.0 0.10609581321571192 0.0 0.0 17 0.0 0.0 0.1377506296177768 0.0 0.0 18 0.0 0.0 0.1593176473862166 0.0 0.0 19 0.0 0.0 0.18053681002935898 0.0 0.0 20 0.0 0.0 0.22088800456385924 0.0 0.0 21 0.0 0.0 0.2953290013775063 0.0 0.0 22 0.0 0.0 0.36281289568520503 0.0 0.0 23 0.0 0.0 0.5370883134592105 0.0 0.0 24 0.0 0.0 0.744757823261768 0.0 0.0 25 0.0 0.0 0.893639816889062 0.0 0.0 26 0.0 0.0 1.0435653758922483 0.0 0.0 27 3.4785512529741616E-4 0.0 1.339937942645647 0.0 0.0 28 3.4785512529741616E-4 0.0 1.6088299545005496 0.0 0.0 29 3.4785512529741616E-4 0.0 1.8074552310453742 0.0 0.0 30 3.4785512529741616E-4 0.0 2.0377353239922638 0.0 0.0 31 3.4785512529741616E-4 0.0 2.6036956128511597 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAG 15 0.008016547 50.6259 23 TAAACCC 20 4.1607153E-4 50.035732 25 CACGAGG 15 0.008396971 50.035732 24 ATCGTGG 50 3.783498E-10 45.032158 26 TCCTAGG 40 1.495373E-7 43.781265 25 ATCCTAG 45 3.76951E-7 38.91668 24 TGCAGTG 145 0.0 38.405853 23 AGTTGCA 150 0.0 37.116142 20 TCCCTGA 35 1.4074703E-4 36.161358 23 GATACGG 55 1.6370905E-11 35.989975 4 GGTACCA 705 0.0 35.989975 4 GTACCAT 160 0.0 35.989975 5 GTACCAC 205 0.0 35.989975 5 GGTAAGG 30 1.0021107E-5 35.989975 4 GTACACG 335 0.0 35.989975 5 AGATACT 40 4.8677975E-8 35.989975 3 CGGTTCC 55 1.6370905E-11 35.989975 3 GGTCCTA 30 1.0021107E-5 35.989975 4 GGTCCCA 25 1.4488879E-4 35.989975 4 ATACTCG 40 4.8677975E-8 35.989975 5 >>END_MODULE