FastQCFastQC Report
Thu 26 May 2016
SRR2099055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476927
Sequences flagged as poor quality0
Sequence length43
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACAGTCGTACAATCGCCAGCCCTGCTCCGCCTGAGAAGAA7330.1536922841441143No Hit
TCGACAGTAGTGTGGAAAAGGAAAAGGAAAAGGAAAAGGAAAG5900.12370865981586279No Hit
TCGACAGTCACCAGTCAAGAGACAGAGACCATCCTGGCCAACA5790.12140222717522808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTG150.00678447352.81567425
ATCTCGG651.8189894E-1244.69018624
AACTTCA900.041.61910222
AGCCCTG1400.040.13270621
AGCTGTC601.1768861E-940.13270622
CTGCTCC1550.037.4820925
TGCTCCG1550.037.4820926
CCTGCTC1650.036.81092524
GCCCTGC1600.036.78831522
CCTTCGA752.6375346E-1035.6735223
GCCCCCG300.002200907835.67351521
AAACTTC1050.035.67351521
TCCGTAG300.002200907835.67351522
GCTCCTC451.0650265E-535.67351521
ACGGTAA301.1673848E-535.204744
ACCGTAC650.035.2047354
CGCTAGT200.002387388635.090882
CGCCAAT700.035.0908782
ACGCGAA301.2202963E-534.9777610
CTTTACG301.2202963E-534.977769
Produced by FastQC (version 0.11.5)