##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099055_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 476927 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.8251136966454 30.0 26.0 31.0 17.0 35.0 2 29.172646966936238 30.0 28.0 32.0 23.0 35.0 3 28.731095115185344 30.0 27.0 32.0 20.0 35.0 4 24.58133844382893 27.0 19.0 31.0 10.0 34.0 5 28.15119504662139 30.0 27.0 31.0 18.0 37.0 6 24.664221149148613 27.0 18.0 31.0 10.0 34.0 7 26.952401520568138 29.0 24.0 31.0 15.0 35.0 8 26.607958870015747 29.0 24.0 31.0 13.0 37.0 9 25.658002168046682 27.0 26.0 28.0 20.0 31.0 10 25.875156994676335 27.0 26.0 28.0 20.0 31.0 11 25.41728818037142 27.0 26.0 27.0 16.0 31.0 12 27.870479129929738 30.0 26.0 31.0 18.0 35.0 13 27.5304983781585 30.0 25.0 31.0 17.0 35.0 14 30.80139937558578 31.0 31.0 31.0 28.0 34.0 15 30.717271196640155 31.0 31.0 33.0 27.0 34.0 16 30.977826795295716 31.0 31.0 33.0 28.0 34.0 17 34.87579231203098 35.0 35.0 37.0 32.0 37.0 18 34.943817397631086 35.0 35.0 37.0 32.0 37.0 19 34.753836541022004 35.0 35.0 37.0 32.0 37.0 20 34.74091213120666 35.0 35.0 37.0 32.0 37.0 21 34.73515443663286 35.0 35.0 37.0 32.0 37.0 22 36.252694856864885 37.0 35.0 39.0 32.0 39.0 23 36.06348980032583 37.0 35.0 39.0 32.0 39.0 24 36.32193606149369 37.0 35.0 39.0 32.0 39.0 25 36.48963258528035 37.0 35.0 39.0 32.0 39.0 26 22.603396326901183 32.0 2.0 37.0 2.0 39.0 27 29.500491689503843 34.0 17.0 38.0 17.0 40.0 28 34.36801019862578 36.0 32.0 39.0 27.0 40.0 29 36.37811447034871 37.0 34.0 39.0 32.0 40.0 30 37.15328761005353 38.0 36.0 40.0 33.0 40.0 31 37.51773541862801 38.0 37.0 40.0 33.0 41.0 32 37.86346547794526 39.0 37.0 40.0 34.0 41.0 33 37.94300595269297 39.0 37.0 40.0 34.0 41.0 34 37.97564616807184 39.0 37.0 40.0 34.0 41.0 35 38.00720655362351 39.0 37.0 40.0 34.0 41.0 36 38.00566543726818 39.0 37.0 40.0 34.0 41.0 37 38.04734686859834 39.0 37.0 40.0 34.0 41.0 38 37.99868952690873 39.0 37.0 40.0 33.0 41.0 39 37.8799019556452 39.0 37.0 40.0 33.0 41.0 40 37.8171879553894 39.0 37.0 40.0 33.0 41.0 41 37.776003455455445 39.0 37.0 40.0 33.0 41.0 42 37.68500420399768 39.0 37.0 40.0 33.0 41.0 43 37.65240802051467 39.0 37.0 40.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.0391387411881965 1101 2 5.153153093563176 1101 3 5.425430106429786 1101 4 7.367627370135995 1101 5 8.236570826262376 1101 6 6.1793679282191825 1101 7 6.547210370828935 1101 8 5.61356111940367 1101 9 5.873573658834395 1101 10 5.4372864481627055 1101 11 3.276884382779116 1101 12 7.723467662626831 1101 13 6.52665280915739 1101 14 0.05897664284535864 1101 15 1.0508008007757823 1101 16 1.5023707160004776 1101 17 0.6942302773482112 1101 18 0.21087806624100836 1101 19 0.11596023524587906 1101 20 -0.05913690040650721 1101 21 0.08836844705142965 1101 22 0.07453812050924569 1101 23 1.009295680055729 1101 24 0.4038258958724441 1101 25 0.33655670064286625 1101 26 12.467691325646221 1101 27 7.281195421831455 1101 28 2.9471563805273817 1101 29 1.2320979928575895 1101 30 0.5493676885322145 1101 31 0.19937277147312216 1101 32 -0.08780985736224522 1101 33 -0.27578960088246873 1101 34 0.028528527473838494 1101 35 0.12385602295242393 1101 36 -0.018344743659710616 1101 37 -0.22309975434509965 1101 38 -0.10997613452743593 1101 39 -0.12241245051235694 1101 40 -0.3677360573803199 1101 41 -0.23280955196053554 1101 42 -0.2616620097529889 1101 43 -0.08785842182445691 1102 1 3.4918968030224704 1102 2 2.5711039843365135 1102 3 1.620498349303503 1102 4 3.929937230645276 1102 5 5.261943192155567 1102 6 2.661789253090518 1102 7 3.9458649454954333 1102 8 1.7081980488850306 1102 9 4.45736344714426 1102 10 3.650747713094905 1102 11 4.0940750160866095 1102 12 5.806424873854201 1102 13 4.333035365906252 1102 14 -0.2552318580150654 1102 15 1.1753576177510574 1102 16 1.5522373954883513 1102 17 0.7663473590012657 1102 18 0.3283427528326328 1102 19 0.06959434606517334 1102 20 0.06198423394971542 1102 21 -0.045237937909298864 1102 22 0.034919391130848965 1102 23 0.9431947491159534 1102 24 0.6061555491189097 1102 25 0.48270262600340885 1102 26 12.414186428546415 1102 27 7.088114866593095 1102 28 2.937353967335966 1102 29 1.2770385244969162 1102 30 0.6170320053121685 1102 31 0.3317968008176564 1102 32 0.16148387447020696 1102 33 0.05036986687720457 1102 34 0.11418780792369887 1102 35 0.06580180126975677 1102 36 -0.07262231876893566 1102 37 -0.09493877608706214 1102 38 -0.03666908466696839 1102 39 -0.06363022471087731 1102 40 -0.06554142746243485 1102 41 -0.09716719950958463 1102 42 0.028281156243572525 1102 43 0.03365976926360048 1103 1 -0.18576448394236422 1103 2 -0.14394863659862978 1103 3 0.2977616605372866 1103 4 0.8280065076217866 1103 5 1.7641434139621488 1103 6 -2.0322885327801536 1103 7 -0.33306169881754144 1103 8 -1.8174927445196012 1103 9 2.133519077284216 1103 10 1.2355227621794782 1103 11 -1.704255648754561 1103 12 1.7785887425243736 1103 13 1.367413493679173 1103 14 -0.6579043515655343 1103 15 1.2573076339458602 1103 16 1.6476974981509507 1103 17 0.8458543665644243 1103 18 0.397554301266446 1103 19 0.14306034158038017 1103 20 0.24067452609958906 1103 21 0.21170703599788254 1103 22 0.3950612024287139 1103 23 1.1473749542573017 1103 24 0.8287448354608244 1103 25 0.695037007808132 1103 26 12.583277448732332 1103 27 7.290946766727139 1103 28 3.115285888612469 1103 29 1.4011509872475258 1103 30 0.6036941406790746 1103 31 0.21170600178945875 1103 32 0.2633129406493495 1103 33 0.04566796746232171 1103 34 0.228076998718997 1103 35 0.1934846903140155 1103 36 0.27303744766905425 1103 37 0.22453705310564942 1103 38 0.22196350100190188 1103 39 0.1415432975744082 1103 40 -0.10523599190341315 1103 41 0.10311841296478974 1103 42 -0.09169338628711188 1103 43 -0.1266998472305474 1104 1 1.194228006200948 1104 2 -0.5104137129203927 1104 3 -0.6900769230582284 1104 4 1.011562197191619 1104 5 0.9429047427516402 1104 6 -0.7138660578272322 1104 7 0.253554826280741 1104 8 -0.619438480115754 1104 9 2.234235927240693 1104 10 1.1702080593593003 1104 11 0.8803980728914631 1104 12 2.8177133890775217 1104 13 1.2005752002372105 1104 14 0.10436373893961814 1104 15 1.9131303330566247 1104 16 1.797425834774586 1104 17 0.8383126498794553 1104 18 0.192196701234586 1104 19 0.06632382945366544 1104 20 0.06654655131450227 1104 21 -0.027071133076304932 1104 22 0.16823792870012255 1104 23 0.9125838363696204 1104 24 0.48228214863891594 1104 25 0.1995628343190674 1104 26 12.337579401863998 1104 27 7.103049014563787 1104 28 2.870190337064699 1104 29 1.1047198759594181 1104 30 0.5155102174616673 1104 31 0.15165445686401569 1104 32 -0.004977827347282471 1104 33 -0.1197855539208561 1104 34 -0.0630972128882803 1104 35 -0.05334609327125861 1104 36 -0.13815166986589134 1104 37 -0.20737983781368285 1104 38 -0.1614863678600713 1104 39 -0.09854170034900278 1104 40 -0.3993845619015346 1104 41 -0.34558699014952055 1104 42 -0.3712967293111973 1104 43 -0.3781520373209517 1105 1 0.9404807792453518 1105 2 -1.5722218401691528 1105 3 -0.8537945416451507 1105 4 -1.1419550022600582 1105 5 -1.9302364041123 1105 6 -2.4104127224284255 1105 7 -4.896774139355429 1105 8 -2.6669977626170045 1105 9 1.6893489973040907 1105 10 0.6383157835484319 1105 11 0.9823262704052951 1105 12 2.7956772218559998 1105 13 0.3052318688426965 1105 14 0.14582510045692487 1105 15 1.6602459284537368 1105 16 1.5609265786373534 1105 17 0.6777762502921618 1105 18 0.07005852689886893 1105 19 -0.13181169094337264 1105 20 -0.2711390855577065 1105 21 -0.08900581929481888 1105 22 -0.06133361219186639 1105 23 0.5822751552426908 1105 24 0.3199572632873142 1105 25 0.31968717193046103 1105 26 12.164198582124307 1105 27 6.6500532497588765 1105 28 2.808078234461327 1105 29 0.9818182558292321 1105 30 0.24633565669144275 1105 31 -0.029972571221556166 1105 32 0.006184206130932068 1105 33 -0.17899439960367403 1105 34 -0.31584161345764983 1105 35 -0.3180943052239158 1105 36 -0.28040599786114484 1105 37 -0.3042611675889404 1105 38 -0.2575580709696794 1105 39 -0.11679483582555861 1105 40 -0.346209627007795 1105 41 -0.14493256628512086 1105 42 -0.21822701665558952 1105 43 -0.3419877386417838 1106 1 -5.18275650949057 1106 2 -1.7775877324358973 1106 3 -7.464073833731476 1106 4 -0.9407152394206726 1106 5 0.6934453749333827 1106 6 -1.3028198309500887 1106 7 -4.2522094053434145 1106 8 -3.325522946830141 1106 9 -0.8216281423821812 1106 10 0.1338934001271106 1106 11 0.6815132444997971 1106 12 2.6155018832191033 1106 13 -4.351039161364717 1106 14 0.13794321375407037 1106 15 1.865656422797798 1106 16 1.6906858279931392 1106 17 0.8010598902064743 1106 18 0.13076807598498874 1106 19 -0.06914314865732507 1106 20 -0.04396846206002181 1106 21 -0.05125453359621446 1106 22 0.00702781680621456 1106 23 1.0265686911335905 1106 24 0.5453232517546169 1106 25 0.3415400034408691 1106 26 12.193726031860606 1106 27 6.860092302813044 1106 28 2.786228463877798 1106 29 1.1865229878909958 1106 30 0.4285758091430907 1106 31 0.2811575273534075 1106 32 -0.03768324873898621 1106 33 -0.05375307831523202 1106 34 5.655230496302011E-4 1106 35 -0.2273317015295504 1106 36 -0.39595598738840465 1106 37 -0.2789731015743726 1106 38 -0.20241241239158114 1106 39 -0.2342837038904264 1106 40 -0.2302665270147486 1106 41 -0.18371757071690098 1106 42 -0.08586554218087628 1106 43 -0.2367211515883625 1107 1 2.080443545048066 1107 2 0.6492842332437192 1107 3 -4.018892468415125 1107 4 -20.442504788454315 1107 5 -23.464750759640705 1107 6 -7.93796852177932 1107 7 -8.429630409536074 1107 8 -1.6908052086253385 1107 9 1.4088998597993623 1107 10 1.7372295090875163 1107 11 2.4321182825064085 1107 12 4.320893417069577 1107 13 3.4464496265627673 1107 14 0.37495254862617244 1107 15 1.9430720705171574 1107 16 1.707405328964608 1107 17 0.7894144303045465 1107 18 0.3367463871376515 1107 19 -0.17924789205790148 1107 20 -0.057917205480080725 1107 21 0.40902234737807674 1107 22 0.20018467869198986 1107 23 1.0683954433090506 1107 24 0.7257414462335561 1107 25 0.715869835588471 1107 26 12.422766395321666 1107 27 7.212484942044831 1107 28 2.9849219713452086 1107 29 1.2451130667285426 1107 30 0.7051889597719878 1107 31 0.631780095636266 1107 32 0.1764953360807695 1107 33 0.6328981032740799 1107 34 0.04274531551063632 1107 35 0.29882980100990153 1107 36 -0.15627815490967123 1107 37 0.1494765889675591 1107 38 0.39287681281890485 1107 39 0.3538229775795827 1107 40 0.32864980974186153 1107 41 0.3164577320455777 1107 42 0.048053594550403034 1107 43 -0.03627461649615782 1113 1 -9.1534500123749 1113 2 -0.5015761108939358 1113 3 0.5561375435896743 1113 4 3.4986716821339208 1113 5 -23.464750759640705 1113 6 -7.126643991967395 1113 7 -0.4800505776033006 1113 8 0.37842248245108934 1113 9 -20.686338235438733 1113 10 -21.483858926286633 1113 11 -10.61129908444037 1113 12 -22.043052161161715 1113 13 -10.8842826663544 1113 14 0.6414591512672274 1113 15 1.7613994014495304 1113 16 1.3754725558553638 1113 17 1.1209470433497657 1113 18 0.6274626736522606 1113 19 -0.3701242425981164 1113 20 -0.5263045604220551 1113 21 0.21894631696591205 1113 22 -0.5683227242691942 1113 23 0.5279792768424585 1113 24 0.31377666031919205 1113 25 0.4853776387097213 1113 26 13.042814414529353 1113 27 7.333333281380561 1113 28 2.747026813281984 1113 29 1.015021029913818 1113 30 0.6127519849820757 1113 31 0.7276184309704021 1113 32 -0.6796471209020183 1113 33 -1.752856198446608 1113 34 -1.10171246760261 1113 35 -1.0905259412870194 1113 36 -0.2867303357820177 1113 37 0.47280592070025307 1113 38 -1.609924307629278 1113 39 0.3014020091922305 1113 40 0.5693460882532619 1113 41 -0.43804406867471357 1113 42 0.20151497910422478 1113 43 -0.11330542882109285 1114 1 -1.5246467669387975 1114 2 -1.3873097146802813 1114 3 -7.844132909419113 1114 4 -1.1379070873048889 1114 5 0.006513608175389152 1114 6 -0.3095378391614467 1114 7 2.20994266107148 1114 8 1.608983672444328 1114 9 -5.025418695208849 1114 10 0.5908537173915285 1114 11 1.4715271495014832 1114 12 -22.043052161161715 1114 13 -8.886649130181311 1114 14 -0.19119804954413055 1114 15 -27.356247657374 1114 16 -13.76550107497628 1114 17 -6.098457960030899 1114 18 -2.89654003087783 1114 19 -1.471882187155252 1114 20 -0.7862775151211778 1114 21 -0.23101987640798427 1114 22 -0.10652421176074256 1114 23 1.3998521639283297 1114 24 0.5338577962218238 1114 25 0.3555601944906499 1114 26 12.512726517301495 1114 27 7.455037135335942 1114 28 3.1456069349858353 1114 29 1.3375362428955668 1114 30 0.5606897807900566 1114 31 0.15899097999000844 1114 32 -0.043068351463212196 1114 33 -0.3130319929023244 1114 34 -0.6179396481299904 1114 35 -0.020546225262677353 1114 36 -0.4138432499131923 1114 37 -0.49643004954991454 1114 38 -0.47740233332230986 1114 39 -0.7185439002060079 1114 40 -0.5249743985621507 1114 41 -0.6905795656321132 1114 42 -0.2197155820857688 1114 43 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0.9581000383473111 1115 38 -0.09521842527633595 1115 39 0.2131667150745855 1115 40 0.260522558841501 1115 41 0.23842651956058347 1115 42 0.3485738026336378 1115 43 0.5337533947083202 1201 1 7.369012464051767 1201 2 6.069143876705844 1201 3 6.204539723961169 1201 4 8.36250179916097 1201 5 8.793483759463381 1201 6 7.626850027511278 1201 7 7.454176604705722 1201 8 6.588993667446825 1201 9 6.727803178702683 1201 10 6.278986923515738 1201 11 5.94761486322038 1201 12 9.14842785640526 1201 13 7.784734874785904 1201 14 0.13134522427350603 1201 15 1.920871358875452 1201 16 1.7465232159085815 1201 17 0.8755777493871193 1201 18 0.23760501780994758 1201 19 0.15476660965845923 1201 20 0.16629922681088516 1201 21 0.09985256615861005 1201 22 0.19777818249666979 1201 23 1.0399790701721798 1201 24 0.4702002310650073 1201 25 0.3951918937925143 1201 26 -4.217590142550399 1201 27 -2.815236818596187 1201 28 -1.1416812391089337 1201 29 -0.5710700600135894 1201 30 -0.11286738002348073 1201 31 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0.41526940861493955 2215 42 -0.1283435465845315 2215 43 -0.026931638798750157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 13.0 25 211.0 26 1614.0 27 6271.0 28 15657.0 29 29045.0 30 46523.0 31 62706.0 32 76997.0 33 84185.0 34 69183.0 35 50301.0 36 21217.0 37 12500.0 38 504.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.781593478832498 1.709808046279253 70.9362082566395 0.572390218248751 2 0.6254625970012182 1.4901651615446387 0.9965885764488066 96.88778366500533 3 72.16450316295784 1.5321003004652702 25.63683750343344 0.666559033143437 4 2.4435289103565845 92.24130802161942 1.128255582437295 4.1869074855866995 5 0.334879205053905 1.9946685683967684 0.5430241375088338 97.12742808904049 6 1.6346419974671018 92.50656289052344 1.085288222022796 4.773506889986664 7 94.96441771478217 1.699282884952364 2.0829783519185825 1.253321048346891 8 1.802162595114137 2.9052664244213475 93.85063122867861 1.4419397517859127 9 27.418496046603153 24.446029871980237 21.82144578363965 26.314028297776964 10 27.50138662453569 28.12158596231743 17.75026471922954 26.62676269391734 11 27.116967935918122 25.23592001969419 19.49409489823615 28.153017146151534 12 33.363961851652356 23.101295903171636 16.430615844449374 27.104126400726635 13 22.817422791922294 20.230184517371153 37.292569366673824 19.659823324032736 14 31.041273849548332 24.951603532784805 21.930153882938228 22.076968734728634 15 27.035692603460486 26.37399237748322 24.89987014739114 21.69044487166515 16 21.097322834645666 24.177847769028872 33.85805774278216 20.866771653543307 17 21.707724662264873 28.273090011678935 27.171244236539344 22.847941089516844 18 20.751947185860306 27.641650693521125 26.071528934112347 25.534873186506225 19 23.347388594061567 28.693280103663664 23.743885332556136 24.215445969718637 20 25.560096199208683 27.31235597900727 24.753893153459543 22.3736546683245 21 23.31803399681712 26.522507637437176 27.03621308921491 23.123245276530792 22 22.48708921910481 26.891117508549527 25.017455501575714 25.604337770769952 23 21.66902422470171 28.62646343036323 25.666300875150917 24.03821146978414 24 22.30236493215943 30.884600787961258 25.200502382964267 21.61253189691504 25 21.76391774841852 29.29819448259377 27.25993705535648 21.677950713631226 26 19.916920880119427 29.666385409229573 27.221068345557214 23.19562536509379 27 21.888996058039083 29.944854482932147 27.160530067935923 21.005619391092843 28 20.126561576106784 31.88676555076092 26.909641408867696 21.077031464264596 29 21.2340040971318 30.021030824940926 27.630478131506607 21.114486946420673 30 22.105572154950988 31.135922839020814 25.450123184987156 21.308381821041046 31 20.211898256965952 32.31249226820876 26.290186967816876 21.18542250700841 32 22.30446168910546 30.524377944633034 26.235671287220057 20.935489079041446 33 22.674539290080034 31.047518802667913 26.308638428941954 19.969303478310096 34 21.75532104493979 31.732319621241828 26.088898300997847 20.423461032820537 35 21.873997488085177 31.0798088596368 27.480096534689796 19.566097117588228 36 22.00294804026612 32.5806674816062 25.67667588540804 19.73970859271964 37 21.557596864929014 34.25849238982067 25.68527258888677 18.498638156363555 38 22.37679980374355 31.438144621713594 27.224292187273942 18.960763387268912 39 21.508113401002667 32.63686056775985 26.09728532878197 19.75774070245551 40 21.029004438834455 33.43237015308422 25.154373730151576 20.384251677929747 41 21.843384836673117 33.86283435410451 25.372646128233463 18.921134680988917 42 21.962899982596916 33.86472143535593 25.63159561106836 18.540782970978785 43 21.2313414841265 33.37701576970899 26.075269380848642 19.31637336531587 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 6.0 9 12.0 10 53.0 11 94.0 12 94.0 13 216.5 14 339.0 15 543.5 16 748.0 17 1101.0 18 1454.0 19 1454.0 20 1936.0 21 2418.0 22 3142.5 23 3867.0 24 4687.5 25 5508.0 26 5508.0 27 7649.0 28 9790.0 29 12121.5 30 14453.0 31 16609.0 32 18765.0 33 18765.0 34 21282.0 35 23799.0 36 26975.0 37 30151.0 38 32152.0 39 34153.0 40 34153.0 41 35232.5 42 36312.0 43 36716.0 44 37120.0 45 39815.5 46 42511.0 47 42511.0 48 44730.0 49 46949.0 50 46053.0 51 45157.0 52 42774.0 53 40391.0 54 40391.0 55 35312.0 56 30233.0 57 25895.5 58 21558.0 59 17117.0 60 12676.0 61 12676.0 62 10195.0 63 7714.0 64 6106.0 65 4498.0 66 3938.5 67 3379.0 68 3379.0 69 2589.0 70 1799.0 71 1195.0 72 591.0 73 417.0 74 243.0 75 243.0 76 200.0 77 157.0 78 118.5 79 80.0 80 42.0 81 4.0 82 4.0 83 3.5 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.3254166780241001 2 0.0 3 0.0 4 1.1142166411211778 5 2.387367458751549 6 6.290270838094718E-4 7 0.3249973266348938 8 0.0 9 0.37028727666917577 10 0.19940158556760257 11 0.34806165304124115 12 0.7363810394462884 13 1.3310213093408425 14 0.028515894466029392 15 0.533205291375829 16 0.1419504452463375 17 0.0 18 0.017822434041268372 19 0.0 20 0.0 21 0.0 22 0.0 23 0.834509264520566 24 0.0 25 0.0 26 35.39053146498311 27 0.001467729862222101 28 0.0010483784730157865 29 0.0016774055568252585 30 4.1935138920631463E-4 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 476927.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.23753254745927 #Duplication Level Percentage of deduplicated Percentage of total 1 77.56321154994201 36.638947300758545 2 8.580482093627776 8.106416043412668 3 4.136867295449396 5.86246210539932 4 2.7356389145462496 5.168993290558984 5 1.8302789896000728 4.322893167108215 6 1.2480551810458131 3.537302834140605 7 0.8235636452339247 2.72321801476492 8 0.6169815147517695 2.331574750741395 9 0.3877029143078147 1.6482716130024186 >10 1.811310338481943 15.430856882379809 >50 0.1667555903649311 5.459910456757864 >100 0.0977999002939596 8.364226858893838 >500 0.0013520723542943724 0.4049266820814519 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGACAGTCGTACAATCGCCAGCCCTGCTCCGCCTGAGAAGAA 733 0.1536922841441143 No Hit TCGACAGTAGTGTGGAAAAGGAAAAGGAAAAGGAAAAGGAAAG 590 0.12370865981586279 No Hit TCGACAGTCACCAGTCAAGAGACAGAGACCATCCTGGCCAACA 579 0.12140222717522808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.096756946031573E-4 0.0 0.0 0.0 0.0 7 2.096756946031573E-4 0.0 0.0 0.0 0.0 8 2.096756946031573E-4 2.096756946031573E-4 0.0 0.0 0.0 9 2.096756946031573E-4 2.096756946031573E-4 0.0 0.0 0.0 10 2.096756946031573E-4 2.096756946031573E-4 4.193513892063146E-4 0.0 0.0 11 2.096756946031573E-4 2.096756946031573E-4 0.0031451354190473596 0.0 0.0 12 2.096756946031573E-4 2.096756946031573E-4 0.003564486808253674 0.0 0.0 13 2.096756946031573E-4 2.096756946031573E-4 0.009435406257142079 0.0 0.0 14 2.096756946031573E-4 2.096756946031573E-4 0.026628813214600977 0.0 0.0 15 2.096756946031573E-4 2.096756946031573E-4 0.037531949333965156 0.0 0.0 16 2.096756946031573E-4 2.096756946031573E-4 0.06395108685396297 0.0 0.0 17 2.096756946031573E-4 2.096756946031573E-4 0.10504752299618181 0.0 0.0 18 2.096756946031573E-4 2.096756946031573E-4 0.12433768689967227 0.0 0.0 19 2.096756946031573E-4 2.096756946031573E-4 0.14572460774919432 0.0 0.0 20 2.096756946031573E-4 2.096756946031573E-4 0.20464347793268153 0.0 0.0 21 2.096756946031573E-4 2.096756946031573E-4 0.25496564463743926 0.0 0.0 22 2.096756946031573E-4 2.096756946031573E-4 0.32478765094029066 0.0 0.0 23 2.096756946031573E-4 2.096756946031573E-4 0.4036257121110778 0.0 0.0 24 2.096756946031573E-4 2.096756946031573E-4 0.5195763712266238 0.0 0.0 25 2.096756946031573E-4 2.096756946031573E-4 0.6076401629599498 0.0 0.0 26 2.096756946031573E-4 2.096756946031573E-4 0.747913202649462 0.0 0.0 27 2.096756946031573E-4 2.096756946031573E-4 0.9259278673675426 0.0 0.0 28 2.096756946031573E-4 2.096756946031573E-4 1.0347495528665813 0.0 0.0 29 2.096756946031573E-4 2.096756946031573E-4 1.3111021183535425 0.0 0.0 30 2.096756946031573E-4 2.096756946031573E-4 1.5518098157579672 0.0 0.0 31 2.096756946031573E-4 2.096756946031573E-4 1.7405179409008087 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGTTG 15 0.006784473 52.815674 25 ATCTCGG 65 1.8189894E-12 44.690186 24 AACTTCA 90 0.0 41.619102 22 AGCCCTG 140 0.0 40.132706 21 AGCTGTC 60 1.1768861E-9 40.132706 22 CTGCTCC 155 0.0 37.48209 25 TGCTCCG 155 0.0 37.48209 26 CCTGCTC 165 0.0 36.810925 24 GCCCTGC 160 0.0 36.788315 22 CCTTCGA 75 2.6375346E-10 35.67352 23 GCCCCCG 30 0.0022009078 35.673515 21 AAACTTC 105 0.0 35.673515 21 TCCGTAG 30 0.0022009078 35.673515 22 GCTCCTC 45 1.0650265E-5 35.673515 21 ACGGTAA 30 1.1673848E-5 35.20474 4 ACCGTAC 65 0.0 35.204735 4 CGCTAGT 20 0.0023873886 35.09088 2 CGCCAAT 70 0.0 35.090878 2 ACGCGAA 30 1.2202963E-5 34.97776 10 CTTTACG 30 1.2202963E-5 34.97776 9 >>END_MODULE