FastQCFastQC Report
Thu 26 May 2016
SRR2099053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726083
Sequences flagged as poor quality0
Sequence length43
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGTCGGTACTGTCGGGCACCATGGCCATCTCCGAGACCG9280.12780907967821858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC150.00790943550.8100222
ATACGCG204.0507977E-450.3230425
TTAACGC250.00121595640.2584326
TGGCCAT2400.039.83907726
GAGCCGT1050.038.34136624
TAGGCGT900.035.9082874
TAGTCCC57800.035.8772244
ACCTCGT200.002158058835.8231851
TAGTCGC49000.035.7250824
CTAGTCC166000.035.71363
TAGTCGA29050.035.661074
CTAGTCA151350.035.6117173
TAGTCTC37650.035.526794
TAGTCCA40550.035.4655234
TAGTCCT34600.035.3893784
GGAGCCG1150.035.34970523
TAGTCAC48500.035.3159834
AGTCGCG8350.035.2632265
TAGTCAT33900.035.1668174
CGGAACG301.1832577E-535.1392758
Produced by FastQC (version 0.11.5)