FastQCFastQC Report
Thu 26 May 2016
SRR2099052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111780
Sequences flagged as poor quality0
Sequence length47
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTTT150.004579428658.28355425
AGTCCTT150.004579428658.28355424
AGACGAA150.00508335456.76809730
TGACCTA850.049.803335
TGACCGG402.7230271E-949.803335
TGACCGC451.382432E-1049.803335
TGACTGC402.7230271E-949.803335
TGACCCG402.7230271E-949.803335
TGACCAT850.049.803335
TGACTAT402.7230271E-949.803335
TGACTAG1050.049.803335
TGACTAA1900.049.803335
TGACAGA2850.049.803335
CTGACGG4550.049.803334
CTGACCG2700.049.803334
CTGACCA5250.049.803334
CTGACAT3100.049.803334
CTGGCTA150.00853485849.803334
TGCCCGA204.2424453E-449.803335
TGCCCCA150.00853485849.803335
Produced by FastQC (version 0.11.5)