FastQCFastQC Report
Thu 26 May 2016
SRR2099051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173177
Sequences flagged as poor quality0
Sequence length47
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGAACTCCTGAGTCCGACGATCGTCGGACTGTAGAACTCTG12550.7246920780473158Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGAACTCCTGAGTCCGACGATCGNCGGACTGTAGAACTCTG7390.4267310324119254Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTATAGTGTGAAGTCGAGTCCGACGATCGTCGGACTGTAGAACTCTG5000.28872194344514573Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGTGAAGTCGAGTCCGACGATCGNCGGACTGTAGAACTCTG2920.1686136149719651Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTATAGTGAATCTCGGGCGAGATTAGCTGATCGTCGGACTGTAGAAC1960.11317900183049712No Hit
CTATAGTGTCATTCTGGACCGCCTCACTATAGGTACCCAGAATGATG1880.10855945073537478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGA1700.051.2924845
ACTGGGC1950.051.2924845
ACTGCGG1000.051.292485
ACTGCCA1050.051.292485
ACTGCAT750.051.292485
GTCTGGA203.675578E-451.292484
ACTGAGC2050.051.292485
ACTGACT2000.051.292485
AACTGCA203.675578E-451.292484
GACTGAG7650.051.292484
ACTGGCC900.051.292485
GACTTAA150.007605510351.2924774
AATGCCT150.007605510351.2924775
GCCTGCA600.051.2924774
ACTTTTC150.007605510351.2924775
GACTGCG5050.051.2924774
GACTGGG7550.050.613114
ACTGACA3050.050.451625
ACTGAGA2250.050.1526455
TAGTGTG2250.050.1526454
Produced by FastQC (version 0.11.5)