##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173177
Sequences flagged as poor quality	0
Sequence length	47
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.899126327399134	33.0	27.0	37.0	17.0	38.0
2	30.210605334426628	31.0	27.0	37.0	18.0	39.0
3	24.233524082297304	27.0	18.0	32.0	10.0	34.0
4	27.945425778249998	30.0	25.0	34.0	16.0	37.0
5	20.497052148957426	24.0	10.0	31.0	2.0	35.0
6	27.45754343821639	30.0	18.0	34.0	16.0	37.0
7	26.53097120287336	30.0	18.0	36.0	8.0	39.0
8	23.64823850742304	30.0	7.0	37.0	2.0	38.0
9	31.079918233945616	32.0	30.0	35.0	24.0	37.0
10	30.88751970527264	32.0	30.0	35.0	25.0	37.0
11	30.899905876646436	32.0	29.0	35.0	23.0	37.0
12	30.4462890568609	32.0	28.0	35.0	23.0	36.0
13	30.992337319620965	32.0	28.0	37.0	22.0	39.0
14	30.453922865045588	32.0	28.0	37.0	18.0	39.0
15	30.818925145948942	32.0	28.0	37.0	18.0	39.0
16	30.315186196781326	32.0	27.0	37.0	17.0	39.0
17	29.964492975395117	31.0	27.0	35.0	17.0	39.0
18	30.834019529152254	31.0	31.0	31.0	28.0	34.0
19	30.89711104823389	31.0	31.0	33.0	28.0	34.0
20	31.109084924672445	31.0	31.0	34.0	28.0	34.0
21	34.82326752397836	35.0	35.0	37.0	32.0	37.0
22	34.98494603786877	35.0	35.0	37.0	32.0	37.0
23	34.82631642770114	35.0	35.0	37.0	32.0	37.0
24	34.721065730437644	35.0	35.0	37.0	32.0	37.0
25	34.7878297926399	35.0	35.0	37.0	32.0	37.0
26	36.37131374258706	37.0	35.0	39.0	32.0	39.0
27	36.05502462798177	37.0	35.0	39.0	32.0	39.0
28	36.402212764974564	37.0	35.0	39.0	32.0	39.0
29	36.54766510564336	37.0	35.0	39.0	32.0	39.0
30	22.027336193605386	32.0	2.0	37.0	2.0	39.0
31	29.193830589512466	34.0	17.0	39.0	17.0	40.0
32	34.25875260571554	36.0	32.0	39.0	27.0	40.0
33	36.48407121038013	37.0	35.0	39.0	32.0	40.0
34	37.25922610970279	38.0	36.0	40.0	33.0	40.0
35	37.69091161066424	39.0	37.0	40.0	33.0	41.0
36	37.92695334830838	39.0	37.0	40.0	34.0	41.0
37	37.95610849015747	39.0	37.0	40.0	34.0	41.0
38	37.79572922501256	39.0	37.0	40.0	33.0	41.0
39	37.67166540591418	39.0	37.0	40.0	33.0	41.0
40	37.4666959238236	39.0	36.0	40.0	32.0	41.0
41	37.298873406976675	39.0	36.0	40.0	32.0	41.0
42	37.057796358638846	39.0	36.0	40.0	31.0	41.0
43	36.68168983179059	38.0	35.0	40.0	31.0	41.0
44	36.37566766949422	38.0	35.0	40.0	30.0	41.0
45	36.05963263019916	38.0	35.0	40.0	30.0	41.0
46	35.691310046946185	38.0	34.0	40.0	29.0	41.0
47	35.40357553254763	38.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.992358918577075
1101	2	4.397803740134041
1101	3	2.499567718543485
1101	4	3.5586903101116683
1101	5	7.561212507894467
1101	6	6.198563037089823
1101	7	8.081946499874405
1101	8	14.2470423919013
1101	9	2.990697967005545
1101	10	3.5746201920284513
1101	11	3.670257747181175
1101	12	3.4521603905816463
1101	13	4.645205480073432
1101	14	5.305839914920899
1101	15	4.42999855221699
1101	16	4.498626608632755
1101	17	3.9793812501691193
1101	18	0.23119346058212997
1101	19	-0.5992641667811043
1101	20	-0.1052852070387047
1101	21	0.10598181830879128
1101	22	-0.0629542158935692
1101	23	-0.21392088143112886
1101	24	-0.16912950738358035
1101	25	-0.13141864947019144
1101	26	0.004093531895613012
1101	27	1.2884916323070854
1101	28	0.7137770860751473
1101	29	0.26178696736410245
1101	30	16.7074793984814
1101	31	9.768550453744915
1101	32	4.083775357386074
1101	33	1.7082330812960365
1101	34	0.9065303836664356
1101	35	0.38788351435474056
1101	36	-0.03189811073198001
1101	37	-0.2398198876424189
1101	38	-0.19266143358892407
1101	39	-0.24526580508739926
1101	40	-0.15845306251597435
1101	41	-0.3899033164206287
1101	42	-0.3573986936791371
1101	43	-0.5049107648455617
1101	44	-0.5925909437906753
1101	45	-0.6055869833344687
1101	46	-0.4449246517183525
1101	47	-0.45199576234907823
1102	1	6.270967308536946
1102	2	2.0878443714112507
1102	3	2.165686270804379
1102	4	0.5782713947417868
1102	5	8.173611366669181
1102	6	5.249697226073216
1102	7	3.684572967301712
1102	8	9.744441649549135
1102	9	3.0048104563950773
1102	10	2.485540172703274
1102	11	3.118297577126775
1102	12	1.0440240064515969
1102	13	3.5293095348651526
1102	14	4.734824366040119
1102	15	3.8350389638898967
1102	16	4.741955791892991
1102	17	3.7916997965141412
1102	18	0.5221028285430265
1102	19	0.22268245951422472
1102	20	0.14985510218207665
1102	21	0.10701171765831674
1102	22	-0.02566182038712128
1102	23	0.025176661420843516
1102	24	-0.1815107081798928
1102	25	-0.0710973818245435
1102	26	0.10234942558203386
1102	27	1.3949553487468478
1102	28	0.6651212884768114
1102	29	0.42283893470490597
1102	30	16.857060850612594
1102	31	9.851925691164055
1102	32	4.253785835648387
1102	33	1.7245868790610928
1102	34	0.8410372106711321
1102	35	0.2944270950521499
1102	36	0.022909744469011173
1102	37	-0.194117991964319
1102	38	-0.23534040885251528
1102	39	-0.49187112596513316
1102	40	-0.21628473447555052
1102	41	-0.446037312690045
1102	42	-0.4976111092995481
1102	43	-0.6439072897195572
1102	44	-0.6276433754377138
1102	45	-0.8840891565206945
1102	46	-0.5324096267506917
1102	47	-0.3475739177267343
1103	1	0.9060372670553463
1103	2	0.285131394739647
1103	3	0.755893412041889
1103	4	-3.2714598418367125
1103	5	3.0727710641602783
1103	6	-7.964101074648376
1103	7	-1.3411616304868268
1103	8	-16.977631630164897
1103	9	-1.9720918951181368
1103	10	-3.010759828630537
1103	11	-1.773467458285971
1103	12	-1.3637081104438167
1103	13	-1.2832709941252816
1103	14	-1.2009191682989062
1103	15	-2.672032915319953
1103	16	-1.6107911970230226
1103	17	-1.491739574992934
1103	18	-0.33915789198302804
1103	19	0.15813789013591517
1103	20	0.13730525090227985
1103	21	0.2503193083909778
1103	22	0.06683814626753559
1103	23	0.15382564172041668
1103	24	0.1365891410991793
1103	25	0.23479940768635288
1103	26	0.14395640409429689
1103	27	1.472049769404947
1103	28	0.892077659437021
1103	29	0.5087712036550656
1103	30	17.14986626056017
1103	31	10.279400915578172
1103	32	4.520403151215319
1103	33	2.0516259464586852
1103	34	0.9757963906425857
1103	35	0.5009361049623848
1103	36	0.17870983250070083
1103	37	-0.18386096610167613
1103	38	-0.05210244318487156
1103	39	-0.13259645374980522
1103	40	0.1967079428883025
1103	41	0.14469001581488072
1103	42	-0.3599122043604339
1103	43	-0.5122372218284497
1103	44	-0.5787404370465623
1103	45	-0.20697472866000055
1103	46	-0.2302208387870266
1103	47	-0.4118050409310925
1106	1	-6.064007856165759
1106	2	-3.919450347710889
1106	3	-2.5131798264169802
1106	4	-4.130085319101553
1106	5	-15.424243861212855
1106	6	-10.256464837230814
1106	7	-5.431477493034553
1106	8	-16.977631630164897
1106	9	-5.788266834375339
1106	10	-2.364907693955079
1106	11	-1.613418864047862
1106	12	-4.0645758646958114
1106	13	-0.7722678702099763
1106	14	-1.8470489843350038
1106	15	-3.4632859524598345
1106	16	-1.4650084826877219
1106	17	-7.658660782906853
1106	18	0.9776071201655299
1106	19	1.1002413264427524
1106	20	0.8003736299373401
1106	21	0.8895418005811919
1106	22	0.631746164316823
1106	23	0.7880151592490563
1106	24	0.8806710571005709
1106	25	0.5869340831462608
1106	26	0.5874482472043283
1106	27	2.3203663261560763
1106	28	1.3177284751260245
1106	29	1.0871467676953301
1106	30	18.134073133173846
1106	31	10.399081582013444
1106	32	4.512176840906399
1106	33	2.4007408371216528
1106	34	1.6996422774874347
1106	35	1.0813942792074371
1106	36	-0.4172984979401164
1106	37	0.5516475378899912
1106	38	0.20343382894286322
1106	39	0.3884726194886525
1106	40	1.1621366134887907
1106	41	0.2291398596191172
1106	42	0.15678338744796605
1106	43	-0.021227155879124382
1106	44	-0.35843498754985603
1106	45	0.2360658822389965
1106	46	0.1493348710359541
1106	47	0.09260315066904923
1107	1	-4.508205589016054
1107	2	-0.7566596500364717
1107	3	0.17818396930582026
1107	4	1.410524707777025
1107	5	-15.424243861212855
1107	6	-8.117397407056686
1107	7	-2.726791271227846
1107	8	-16.977631630164897
1107	9	-1.0181955477094498
1107	10	0.02997368970742187
1107	11	0.056675795295365816
1107	12	-4.320214523104134
1107	13	-26.307151591140208
1107	14	-25.730769914567563
1107	15	-11.823634205352203
1107	16	-6.997613367577287
1107	17	-1.4740166319193584
1107	18	0.5296113272474514
1107	19	0.589256168092863
1107	20	0.4681310215465473
1107	21	0.32380614556191034
1107	22	-0.08316560382621674
1107	23	0.026007446265531087
1107	24	0.10832093439401547
1107	25	0.20449865016740887
1107	26	-0.20798239437566224
1107	27	1.9513117509544884
1107	28	1.1457640015955803
1107	29	0.7065811489036946
1107	30	17.383459600393667
1107	31	10.201991480342294
1107	32	4.417868087838741
1107	33	2.1512959382084773
1107	34	1.2120297964777365
1107	35	0.9525523953696435
1107	36	0.18577500913011846
1107	37	-0.06550800727770678
1107	38	0.10450897210051124
1107	39	-0.11316346792515475
1107	40	-0.38180401991076707
1107	41	-0.03280942167105394
1107	42	0.1054219289928966
1107	43	0.05397443346293329
1107	44	0.16622318704403938
1107	45	0.15443680662838233
1107	46	0.387794611599233
1107	47	0.3786847213129647
1201	1	7.450947110899712
1201	2	5.721664049385492
1201	3	4.05898489106297
1201	4	3.6366844080123073
1201	5	9.330573094471152
1201	6	7.429828123292552
1201	7	9.831203422859161
1201	8	14.50213715596227
1201	9	3.7013487053477405
1201	10	4.672574942600011
1201	11	4.290477268944926
1201	12	4.216868773920485
1201	13	5.513657657414708
1201	14	5.829923727050936
1201	15	5.228384074873592
1201	16	5.379459859831435
1201	17	4.746176336777694
1201	18	0.3338153918866418
1201	19	0.4437574800476298
1201	20	0.22129714116196553
1201	21	0.27209769967156916
1201	22	0.036045577320365396
1201	23	0.008441291793751304
1201	24	0.11671308783946444
1201	25	0.007431909915091239
1201	26	0.19587455898459183
1201	27	1.467081834512932
1201	28	0.7593424913468425
1201	29	0.4261036167341743
1201	30	0.13613434211770326
1201	31	0.03433721673136958
1201	32	-0.11242548019410492
1201	33	0.03494346292261241
1201	34	0.008722799062915954
1201	35	-0.0069464475927603075
1201	36	3.875709759597612E-4
1201	37	-0.06258152515611215
1201	38	-0.27723113498598195
1201	39	-0.024463707078538732
1201	40	0.33726095815877244
1201	41	-0.10143772697406206
1201	42	-0.026361748547017783
1201	43	-0.11099056493948467
1201	44	-0.2394225754873034
1201	45	-0.22127605440123688
1201	46	-2.1479961870340958E-4
1201	47	-0.21120112409906255
1202	1	5.462283152595113
1202	2	3.180433625451599
1202	3	1.7713886041939269
1202	4	1.8732105157031356
1202	5	3.4636809399440516
1202	6	2.530062597230838
1202	7	5.671214665905399
1202	8	5.598651811628308
1202	9	2.2306589925260703
1202	10	2.500282573242959
1202	11	2.759346778555848
1202	12	2.438434106886021
1202	13	3.4072374182596477
1202	14	3.7153616834078527
1202	15	2.8944718709439563
1202	16	3.6652526617740975
1202	17	2.260843496633747
1202	18	0.17647543811269628
1202	19	0.4256797901801619
1202	20	0.19045922074935717
1202	21	0.2477788652703552
1202	22	-0.13721749742290257
1202	23	-0.13918220408309878
1202	24	-0.2314545881936212
1202	25	-0.07493176292480541
1202	26	0.06916981642527276
1202	27	1.4774565748570794
1202	28	0.7986597166131801
1202	29	0.4511935820019417
1202	30	8.331587804683373
1202	31	4.879289757701613
1202	32	1.8946954640545997
1202	33	0.7606005959876114
1202	34	0.39056527938758734
1202	35	0.18962714002567793
1202	36	-0.13663966728885413
1202	37	-0.09736287089441475
1202	38	-0.18045525612667035
1202	39	-0.0639731926151299
1202	40	0.09042698326127407
1202	41	-0.11551870198440639
1202	42	-0.24008725103595197
1202	43	-0.23485946851259598
1202	44	-0.28013368771296854
1202	45	-0.2832214775619093
1202	46	-0.07055280153294774
1202	47	-0.14666436686109563
1203	1	4.37269124207819
1203	2	-1.320435160240553
1203	3	-3.339057852045837
1203	4	-1.1804531406487762
1203	5	2.432898995930003
1203	6	-0.36621804083783616
1203	7	0.5743957865098217
1203	8	5.588694900447347
1203	9	1.3590990887380912
1203	10	2.712275504905861
1203	11	1.9961563613911508
1203	12	1.9510268880331871
1203	13	3.1953994292679546
1203	14	3.264128044616111
1203	15	1.5408668714604303
1203	16	1.8142392629221504
1203	17	0.4198275891861698
1203	18	-1.2214911237832098
1203	19	-1.2095593380090754
1203	20	-0.22424668330423714
1203	21	0.12868513233249246
1203	22	0.10700719896324529
1203	23	-0.03999860439594016
1203	24	0.27631652458988754
1203	25	-0.15843848448544406
1203	26	0.12666277022284333
1203	27	1.20640097257278
1203	28	0.3002866146609051
1203	29	0.7623959371305418
1203	30	16.812941192025296
1203	31	9.925007068487098
1203	32	4.303764406165065
1203	33	1.633951563272575
1203	34	0.6782255712700618
1203	35	0.003380515562440678
1203	36	0.004806390526901794
1203	37	-0.38089873643368577
1203	38	-0.6599382640803952
1203	39	-0.6370969106489781
1203	40	-0.6842089014613819
1203	41	-0.45738126045682037
1203	42	-0.41422990158858397
1203	43	-0.7747867192393514
1203	44	-0.9010904218165905
1203	45	-0.5031367755683149
1203	46	-0.023778560383128422
1203	47	-0.02165883319426598
2101	1	5.306630633794619
2101	2	1.6804359646292362
2101	3	-0.020895540392203316
2101	4	0.857526069726962
2101	5	3.7907094098151823
2101	6	3.5371586225521767
2101	7	4.586428313395487
2101	8	-1.9179623275049416
2101	9	2.2396284392273884
2101	10	3.249590826433465
2101	11	2.508695057674842
2101	12	2.864143644440997
2101	13	3.4361985166958817
2101	14	4.1750532342462385
2101	15	2.6786146377128723
2101	16	2.456555391386477
2101	17	1.9936054468425333
2101	18	0.40345935964524315
2101	19	0.2815313470068439
2101	20	-0.7855023502007583
2101	21	-0.30080143583898433
2101	22	0.03894025255851119
2101	23	0.1523255934356058
2101	24	0.3129349462216595
2101	25	0.2899835874346266
2101	26	0.4994657016038744
2101	27	1.7387536332632294
2101	28	1.1509336311957696
2101	29	0.6756392358878571
2101	30	-3.9893281341091846
2101	31	-2.1602366264027886
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2204	18	-0.03977601349667381
2204	19	-0.006817635267371003
2204	20	0.17822187987861327
2204	21	0.2585810300855371
2204	22	0.30786784625246355
2204	23	0.5101199278654533
2204	24	0.4659423749300231
2204	25	0.6335631646590585
2204	26	0.799745639734283
2204	27	1.757704827448066
2204	28	1.0376603520346421
2204	29	0.7569846817192882
2204	30	-17.842671052872664
2204	31	-10.648663308754706
2204	32	-4.165236830693317
2204	33	-1.5741240912316528
2204	34	-0.3218517323786685
2204	35	0.10147884580362643
2204	36	0.6050176871667645
2204	37	0.5981004389940594
2204	38	0.8009203217781931
2204	39	0.7812962041727047
2204	40	1.1556179383654595
2204	41	0.9003284901100699
2204	42	1.1393415269828466
2204	43	1.1618242888002257
2204	44	0.9226314911910407
2204	45	0.4820724596409178
2204	46	0.7797671003053921
2204	47	0.711231292553002
2205	1	-3.560131887173508
2205	2	-2.100197065002458
2205	3	0.0801598350289936
2205	4	0.10951284574578324
2205	5	6.341062261236125
2205	6	3.9858227754886926
2205	7	5.51062027630574
2205	8	9.51556564874667
2205	9	0.6278065717312913
2205	10	1.6842142804160645
2205	11	0.8711563613911508
2205	12	1.240992874427743
2205	13	1.805093306818975
2205	14	1.725012398357606
2205	15	0.9405267354060101
2205	16	2.536178038432354
2205	17	1.311834391907258
2205	18	-1.639858470721986
2205	19	-1.4969743039954686
2205	20	-0.8883623295627423
2205	21	-3.5303284731096802
2205	22	-0.6132308962748496
2205	23	-0.4609169717428756
2205	24	-0.19392157064820736
2205	25	-0.05979902870313225
2205	26	0.11900970899834817
2205	27	1.1086118569265224
2205	28	0.8666131452731491
2205	29	0.29811022284482647
2205	30	-17.842671052872664
2205	31	-10.74250993831562
2205	32	-4.385861444175074
2205	33	-1.8209804095165367
2205	34	-1.633849258661911
2205	35	-0.3478099606280338
2205	36	-0.11679224892888129
2205	37	0.17947541322617155
2205	38	0.10961955904885912
2205	39	0.2770187356095235
2205	40	0.5521856563617504
2205	41	-0.1070411244024001
2205	42	0.03900139092842636
2205	43	0.2337166821211909
2205	44	0.3905762448500809
2205	45	0.24856390470379353
2205	46	0.032343888596464865
2205	47	-0.24444794884052357
2206	1	-4.9749388301419835
2206	2	-1.750283454499808
2206	3	-2.323228512871271
2206	4	-0.9252562159702968
2206	5	-15.424243861212855
2206	6	-8.314888582380703
2206	7	-4.9875904851508075
2206	8	-6.755527485637797
2206	9	-1.2678284091473522
2206	10	0.03226997453218061
2206	11	-0.39976688569726804
2206	12	-0.9929672345892975
2206	13	-0.4899358631912172
2206	14	-0.14947342147510767
2206	15	-1.5654410076954797
2206	16	-0.33957289956239833
2206	17	-0.6339715606544267
2206	18	0.5626724885267507
2206	19	0.6155886808158826
2206	20	0.3898010080358496
2206	21	0.2723853860790513
2206	22	0.058098634474745836
2206	23	0.15898369840828508
2206	24	0.16643830124954206
2206	25	0.1870255247101582
2206	26	0.34103794643572627
2206	27	1.626077716809263
2206	28	1.080042193832
2206	29	0.7511237343068018
2206	30	-4.423967545965118
2206	31	-2.729146675901198
2206	32	-0.8813431650249441
2206	33	-0.23785491702410866
2206	34	0.16393063969488253
2206	35	0.12823954525295989
2206	36	0.08645802036055272
2206	37	0.196236421561494
2206	38	0.4137864968122713
2206	39	0.36033580313791447
2206	40	0.3919638630748281
2206	41	0.3934504643691312
2206	42	0.09231708489995327
2206	43	0.08599425627518542
2206	44	-0.2030461137639321
2206	45	0.11413967805245306
2206	46	0.027507522594170553
2206	47	0.08919262747502188
2207	1	-10.284425672484243
2207	2	-9.071913887324609
2207	3	-3.3840509340243905
2207	4	-0.47214286738692834
2207	5	-15.424243861212855
2207	6	-8.389929559343546
2207	7	-20.571012376755483
2207	8	-5.3606824776225235
2207	9	-2.605964902960281
2207	10	-0.3273042252913001
2207	11	-4.137975426917393
2207	12	-1.144709881250801
2207	13	-1.3308804046995313
2207	14	-0.6324648298217994
2207	15	-2.8580435401618445
2207	16	-0.31944285399241323
2207	17	0.6304208095251518
2207	18	0.7699997098383733
2207	19	0.8711912673178013
2207	20	0.7718359870520395
2207	21	0.5536332471716534
2207	22	0.1345841619518282
2207	23	0.3174988013460194
2207	24	0.5602926574159
2207	25	0.564979018108815
2207	26	0.6737312586351578
2207	27	1.9888724357343222
2207	28	1.3757103434744664
2207	29	1.055495210738293
2207	30	13.4420747098392
2207	31	8.565538026633071
2207	32	3.989591109727847
2207	33	1.8115804114220069
2207	34	1.5646403066557397
2207	35	1.2549457179145662
2207	36	0.677419673128071
2207	37	0.4874772580318947
2207	38	0.4760786850721459
2207	39	0.2668723041555836
2207	40	0.681529597327966
2207	41	0.26859591007240624
2207	42	0.0012059337625061062
2207	43	0.23021155125528736
2207	44	0.4312772710232622
2207	45	0.23003513726461478
2207	46	0.43995371218692014
2207	47	0.3988703954463446
2208	1	-3.4122530992947198
2208	2	-0.4912051045819261
2208	3	0.30525569155342325
2208	4	0.990168380687031
2208	5	3.0884834115144173
2208	6	3.604338545433034
2208	7	0.8471694414263347
2208	8	9.418732006198738
2208	9	-3.1638693713732238
2208	10	-26.713744903257403
2208	11	-11.859084825992891
2208	12	-6.723138232560508
2208	13	-0.7107879547765705
2208	14	0.5128664490687989
2208	15	0.30047726107082084
2208	16	-25.87198522687377
2208	17	-11.694263319904739
2208	18	0.6973957036750456
2208	19	0.6827783243285879
2208	20	0.3755956172523476
2208	21	0.5838335553380603
2208	22	0.2758476442322575
2208	23	0.41173608948161444
2208	24	0.2774575264451755
2208	25	0.26063387172976604
2208	26	0.5073213973100366
2208	27	1.7054826052258463
2208	28	1.086650251024544
2208	29	0.879408924143533
2208	30	-17.842671052872664
2208	31	-10.595633005724402
2208	32	-3.790994406450892
2208	33	-1.2555382326457902
2208	34	-0.6903365808635158
2208	35	0.0978424821672661
2208	36	0.5611793033283803
2208	37	0.4363832672768879
2208	38	0.3677890086468807
2208	39	0.38927600215249925
2208	40	0.7013755141230362
2208	41	0.764065863847442
2208	42	0.7111597088010271
2208	43	0.981521258497196
2208	44	0.9137426023021504
2208	45	1.0298502374186995
2208	46	0.8081509386892307
2208	47	0.91628179760351
2209	1	-8.337909664951287
2209	2	-5.312215205592029
2209	3	-2.750905924608194
2209	4	-4.478922528403878
2209	5	-7.090910527879521
2209	6	-7.583338222243732
2209	7	-0.3487901545332619
2209	8	2.189035036501771
2209	9	-6.445889573393426
2209	10	-6.380411569924071
2209	11	-7.933832300740367
2209	12	-4.430208939631214
2209	13	-6.584929368917987
2209	14	-5.119658803456453
2209	15	-4.304371223777665
2209	16	-4.2608741157626575
2209	17	-6.316283521924941
2209	18	0.9905270168063574
2209	19	0.8340914556417189
2209	20	0.592363294020025
2209	21	-0.09495432344981936
2209	22	0.6588779472625603
2209	23	0.005069422814948155
2209	24	0.24372015270780167
2209	25	0.13861366970956368
2209	26	-1.5159109259222845
2209	27	0.8965937163369588
2209	28	0.27250883688312655
2209	29	-0.7177627930281858
2209	30	-1.8426710528726638
2209	31	-0.7016936117850072
2209	32	-0.4051358205923066
2209	33	0.34001732290976605
2209	34	-1.1349830455099834
2209	35	-1.645995901671121
2209	36	-0.26871968657060563
2209	37	-0.6266470357534146
2209	38	-0.11827159741373094
2209	39	0.5615992344757359
2209	40	-1.2357962030486789
2209	41	0.205884045665627
2209	42	0.1671193047606252
2209	43	-0.3558524788765425
2209	44	1.064045632605179
2209	45	0.11591084347930547
2209	46	-1.2783137077754176
2209	47	0.25668583800754874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	6.0
25	79.0
26	673.0
27	2662.0
28	7626.0
29	14997.0
30	22337.0
31	24671.0
32	20738.0
33	19481.0
34	19487.0
35	18496.0
36	13736.0
37	7409.0
38	778.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0947007974500078	0.6929326642683498	0.35974754153265154	98.85261899674899
2	0.08834891469421459	2.3357605224712286	96.9961369003967	0.5797536624378526
3	1.0226531236827061	92.73286868348568	1.529648856372382	4.714829336459229
4	90.42193824815074	1.3333179348296829	7.765465390900639	0.4792784261189419
5	0.8161127117826272	89.25336715422048	1.7335502281531314	8.196969905843753
6	7.71420204978038	2.1259150805270863	0.5633967789165447	89.59648609077598
7	0.6273100678123998	1.678069554591704	96.26759583567744	1.427024541918468
8	96.0769377261474	2.1877737573795466	1.3978289849552465	0.3374595315178061
9	23.945117831507584	34.587199935324044	21.030080094242106	20.437602138926263
10	27.67333239449843	30.60484438811435	18.49856827676853	23.22325494061869
11	25.375194165506965	29.061595939414588	20.605507659793158	24.957702235285286
12	28.30803166702276	26.655964706629632	22.489707062716178	22.54629656363143
13	26.617440019628347	25.109095051436782	25.825880208668018	22.447584720266853
14	23.667853443040688	27.478239125516485	23.176075540896154	25.67783189054667
15	24.939512054280353	25.355853905009383	27.126894759636205	22.57773928107406
16	32.6262732948411	28.874454302210957	19.68326057362813	18.816011829319816
17	29.692742107785676	31.198715764795555	19.62038838875832	19.488153738660447
18	27.241486854872242	25.385806034283604	21.453818786674674	25.918888324169476
19	23.34214902757888	24.624081858918096	22.570679539890055	29.463089573612972
20	22.72876883188876	21.73498790255057	24.943843581999918	30.59239968356075
21	24.295951540909012	25.769010896366147	24.219151503952606	25.71588605877224
22	22.885589378425358	29.011244131048702	21.380019288853468	26.72314720167247
23	21.158698903434058	28.402732464472763	15.938606165945824	34.49996246614735
24	27.946551793829432	26.50236463271682	18.548652534689943	27.00243103876381
25	22.821737297678098	31.576364066821803	22.0722151324945	23.529683503005597
26	21.61776679351184	27.67919527419923	24.454748609803843	26.248289322485086
27	23.67432248367855	26.26814146822601	25.418604105020904	24.63893194307454
28	23.97720251534557	27.634154651021785	25.080120339306028	23.308522494326613
29	20.539101612800774	25.655254450648755	33.7181034432979	20.08754049325257
30	16.197156505484962	27.47956120310405	30.76581764628137	25.557464645129617
31	20.330653916337514	30.318411752477303	28.133300995542005	21.21763333564318
32	21.456659949069447	29.931226433071366	31.37021659920197	17.241897018657212
33	18.388547868316653	32.831907976416645	28.779313172376757	20.000230982889942
34	15.357697615734192	41.851400590147655	25.32668887900818	17.464212915109975
35	16.235989767694324	40.55446162019206	25.296661796889886	17.91288681522373
36	16.47967108796203	40.59719246782194	28.676440866858766	14.246695577357272
37	18.19583431979997	41.64583056641471	27.431471846723294	12.726863267062022
38	15.410245009441207	42.86596949941389	29.365908867805775	12.357876623339127
39	14.465546810488691	45.39286394844581	26.583206776881458	13.558382464184044
40	16.905824676487065	43.83145567829446	25.387897931018554	13.874821714199923
41	14.493841560946317	46.24517112549588	26.305456267287227	12.955531046270579
42	13.854611178158763	49.479434335968406	24.971560888570654	11.69439359730218
43	14.291736200534713	46.10658459264221	25.596932618072838	14.004746588750239
44	14.635315313234438	45.74683705110956	27.379501896903168	12.238345738752837
45	13.126454436790105	46.097922934338854	25.83945905056676	14.93616357830428
46	12.18753067670649	46.90692181987215	27.37661467746872	13.528932825952639
47	14.978316982047268	45.632503161505284	25.696830410504862	13.69234944594259
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	5.5
8	11.0
9	11.0
10	31.0
11	51.0
12	119.0
13	187.0
14	329.0
15	471.0
16	662.5
17	854.0
18	1088.0
19	1322.0
20	1714.5
21	2107.0
22	2831.5
23	3556.0
24	4290.0
25	5024.0
26	5024.0
27	5664.5
28	6305.0
29	6906.0
30	7507.0
31	8008.5
32	8510.0
33	8938.5
34	9367.0
35	9979.0
36	10591.0
37	11126.5
38	11662.0
39	12136.0
40	12610.0
41	12926.5
42	13243.0
43	13243.0
44	13701.5
45	14160.0
46	14468.0
47	14776.0
48	13978.0
49	13180.0
50	13357.5
51	13535.0
52	10907.5
53	8280.0
54	7060.0
55	5840.0
56	4700.0
57	3560.0
58	3560.0
59	2905.0
60	2250.0
61	1833.5
62	1417.0
63	1189.0
64	961.0
65	847.0
66	733.0
67	700.5
68	668.0
69	503.5
70	339.0
71	211.0
72	83.0
73	47.5
74	12.0
75	12.0
76	7.5
77	3.0
78	1.5
79	0.0
80	1.0
81	2.0
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	16.225595777730298
6	1.4014563134827374
7	4.543328502052813
8	24.19605374847699
9	0.0034646633213417486
10	1.5897030206089724
11	0.0
12	0.0
13	1.1525779982330218
14	3.01136987013287
15	0.0011548877737805829
16	1.5897030206089724
17	0.0
18	0.019055648267379616
19	5.774438868902914E-4
20	0.0
21	0.0
22	0.01154887773780583
23	0.0
24	0.0
25	0.0
26	0.0
27	1.6451376337504402
28	0.0
29	0.0
30	38.83367883725899
31	0.0028872194344514573
32	0.0
33	0.0023097755475611657
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
47	173177.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.82235709090651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.90533829715383	58.263025300046934
2	7.95706252138328	10.793334714398513
3	2.2245219963978617	4.526169755888161
4	1.0581259901356186	2.8705839500058774
5	0.6411051743778997	2.17406320347429
6	0.41168778510456244	1.6752981582795607
7	0.32096239904530705	1.523789850856339
8	0.22128267642096147	1.2006330158603173
9	0.1873922121132716	1.143844337340108
>10	1.0326502812484881	11.95425004398229
>50	0.028906233298726693	1.3385406814964833
>100	0.008970899989260008	1.1884618778135443
>500	9.96766665473334E-4	0.4995866482959097
>1k	9.96766665473334E-4	0.84841846226166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTATAGTGAACTCCTGAGTCCGACGATCGTCGGACTGTAGAACTCTG	1255	0.7246920780473158	Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGAACTCCTGAGTCCGACGATCGNCGGACTGTAGAACTCTG	739	0.4267310324119254	Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTATAGTGTGAAGTCGAGTCCGACGATCGTCGGACTGTAGAACTCTG	500	0.28872194344514573	Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGTGAAGTCGAGTCCGACGATCGNCGGACTGTAGAACTCTG	292	0.1686136149719651	Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTATAGTGAATCTCGGGCGAGATTAGCTGATCGTCGGACTGTAGAAC	196	0.11317900183049712	No Hit
CTATAGTGTCATTCTGGACCGCCTCACTATAGGTACCCAGAATGATG	188	0.10855945073537478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.03175941377896603	0.0	0.0
17	0.0	0.0	0.06294138367104177	0.0	0.0
18	0.0	0.0	0.15013541059147578	0.0	0.0
19	0.0	0.0	0.2084572431673952	0.0	0.0
20	0.0	0.0	0.31932646945033116	0.0	0.0
21	0.0	0.0	0.3949716186329593	0.0	0.0
22	0.0	0.0	0.4452092367924147	0.0	0.0
23	0.0	0.0	0.48794008442229625	0.0	0.0
24	0.0	0.0	0.5907250962887681	0.0	0.0
25	0.0	0.0	1.9685062104090034	0.0	0.0
26	0.0	0.0	2.2693544754788455	0.0	0.0
27	0.0	0.0	2.526317005145025	0.0	0.0
28	0.0	0.0	2.851417913464259	0.0	0.0
29	0.0	0.0	3.216939893865814	0.0	0.0
30	0.0	0.0	3.434058795336563	0.0	0.0
31	0.0	0.0	3.6702333450746925	0.0	0.0
32	0.0	0.0	3.8867748026585516	0.0	0.0
33	0.0	0.0	4.079063616993019	0.0	0.0
34	0.0	0.0	4.304844176767123	0.0	0.0
35	0.0	0.0	4.498865322762261	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGA	170	0.0	51.292484	5
ACTGGGC	195	0.0	51.292484	5
ACTGCGG	100	0.0	51.29248	5
ACTGCCA	105	0.0	51.29248	5
ACTGCAT	75	0.0	51.29248	5
GTCTGGA	20	3.675578E-4	51.29248	4
ACTGAGC	205	0.0	51.29248	5
ACTGACT	200	0.0	51.29248	5
AACTGCA	20	3.675578E-4	51.29248	4
GACTGAG	765	0.0	51.29248	4
ACTGGCC	90	0.0	51.29248	5
GACTTAA	15	0.0076055103	51.292477	4
AATGCCT	15	0.0076055103	51.292477	5
GCCTGCA	60	0.0	51.292477	4
ACTTTTC	15	0.0076055103	51.292477	5
GACTGCG	505	0.0	51.292477	4
GACTGGG	755	0.0	50.61311	4
ACTGACA	305	0.0	50.45162	5
ACTGAGA	225	0.0	50.152645	5
TAGTGTG	225	0.0	50.152645	4
>>END_MODULE