FastQCFastQC Report
Thu 26 May 2016
SRR2099050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408130
Sequences flagged as poor quality0
Sequence length47
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG150.0061457254.150654
CCGTACT456.184564E-1154.150655
CCGTACA306.026821E-754.150655
ACGTAAC2500.054.150655
TACGCTA202.8185005E-454.1506464
CCGTAGC352.8105205E-854.1506465
ACGTCCC2250.054.1506465
ACGTACG2000.054.1506465
TACGTAA14350.054.1506424
TACGTGC14050.053.957944
TACGTAC11500.053.915214
ACGTTGC4400.053.53535
TACGTCA12700.053.511074
ACGTACT3550.053.3879625
ACGTGCT3300.053.330185
TACGTCT9750.053.3175624
CAACGTC306.871978E-753.131393
CTACGCT600.053.131393
CTACGCG550.053.1313863
TACGTGT10500.053.119214
Produced by FastQC (version 0.11.5)