FastQCFastQC Report
Thu 26 May 2016
SRR2099049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200400
Sequences flagged as poor quality0
Sequence length42
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAGTATTCGGTGTCTGATCCCCGCAGCGTATTGCAGAC4580.22854291417165667No Hit
CTACGAGTGCCAAGGTTGGCTAGCAGGTACAATCCGAACGCG3370.16816367265469062No Hit
CTACGAGTATTCGGTGTCTGATCCNCGCAGCGTATTGCAGAC3180.15868263473053892No Hit
CTACGAGTTGCTGTGAAACCCCGCCTCTACTAAAAATACAAA2910.14520958083832333No Hit
CTACGAGTCGAGAGGTTCACCACGTCCGCTTGTGCGAACTGG2900.14471057884231536No Hit
CTCCGAGTCCTTGCGACGGCGTCATCGTCATCAAGGCTCAGA2840.14171656686626746No Hit
CTACGAGTCATCTTGGTACAGGACTCATTACAATTGCTTTAG2670.13323353293413173No Hit
CTACGAGTCCCTTACGAATCCGCCACCCCGGCCGACGCCTCC2500.124750499001996No Hit
CTACGAGTGCAGGTTGGAAAATGAGAGAAGAAAAAGAAAGGA2500.124750499001996No Hit
CTACGAGTCGGAGGTGGAAACAAGAGTGAAACTCTGTCTCAA2420.12075848303393215No Hit
CTACGAGTGCCAAGGTTGGCTAGCNGGTACAATCCGAACGCG2290.11427145708582834No Hit
CTACGAGTCGAGAGGTTCACCACGNCCGCTTGTGCGAACTGG2090.10429141716566867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGCC203.7340063E-451.13192719
TCACCTC150.00770174351.13192420
CACCTCT150.00770174351.13192421
TACCGTG150.00810784850.47190523
GCTAGCA950.043.05846419
ACCACGT950.043.05846419
CCACGTC950.043.05846420
CAGGTAC950.042.5026624
AGGTACA950.042.5026625
GCAGGTA950.042.5026623
ACGTCCG1000.040.92127222
CTGGATA250.001119059540.92127222
CATGGTG501.564149E-840.90553720
CACGTCC1050.038.95765321
ATCCCCG800.038.34894621
ATGGTGG553.6243364E-837.1868521
CACCTAT351.3250517E-436.522819
ACCTATA351.3250517E-436.522820
AGCAGGT1150.035.58371722
CGTCCGC1150.035.1108923
Produced by FastQC (version 0.11.5)