##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099049_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 200400 Sequences flagged as poor quality 0 Sequence length 42 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.34433632734531 30.0 27.0 31.0 19.0 35.0 2 28.028013972055888 30.0 27.0 31.0 17.0 35.0 3 26.565394211576848 29.0 24.0 31.0 15.0 35.0 4 28.49560878243513 30.0 27.0 31.0 19.0 35.0 5 27.281157684630738 30.0 25.0 31.0 14.0 37.0 6 21.18130738522954 23.0 15.0 28.0 7.0 31.0 7 25.876457085828342 28.0 23.0 31.0 14.0 34.0 8 25.81637225548902 28.0 22.0 31.0 10.0 35.0 9 25.64259481037924 27.0 26.0 27.0 20.0 31.0 10 25.74296407185629 27.0 26.0 27.0 20.0 31.0 11 25.293473053892214 27.0 26.0 27.0 16.0 31.0 12 27.628537924151697 30.0 26.0 31.0 17.0 35.0 13 30.702335329341317 31.0 30.0 31.0 28.0 34.0 14 30.67439620758483 31.0 31.0 33.0 27.0 34.0 15 30.949785429141716 31.0 31.0 34.0 28.0 34.0 16 34.81763473053892 35.0 35.0 37.0 32.0 37.0 17 34.930743512974054 35.0 35.0 37.0 32.0 37.0 18 34.79084331337325 35.0 35.0 37.0 32.0 37.0 19 34.77934131736527 35.0 35.0 37.0 32.0 37.0 20 34.77524451097804 35.0 35.0 37.0 32.0 37.0 21 36.30130239520958 37.0 35.0 39.0 32.0 39.0 22 36.11749500998004 37.0 35.0 39.0 32.0 39.0 23 36.27461077844311 37.0 35.0 39.0 32.0 39.0 24 36.42632734530938 37.0 35.0 39.0 32.0 39.0 25 22.97870758483034 32.0 2.0 37.0 2.0 39.0 26 29.63934131736527 34.0 17.0 38.0 17.0 40.0 27 34.24704091816367 36.0 32.0 39.0 27.0 40.0 28 36.1315868263473 37.0 34.0 39.0 32.0 40.0 29 36.86474550898203 38.0 36.0 39.0 32.0 40.0 30 37.20669161676647 38.0 36.0 40.0 32.0 41.0 31 37.517829341317366 39.0 37.0 40.0 33.0 41.0 32 37.65950099800399 39.0 37.0 40.0 33.0 41.0 33 37.61034930139721 39.0 37.0 40.0 33.0 41.0 34 37.608358283433134 39.0 37.0 40.0 33.0 41.0 35 37.53692614770459 39.0 36.0 40.0 32.0 41.0 36 37.546761477045905 39.0 36.0 40.0 32.0 41.0 37 37.438832335329344 39.0 36.0 40.0 32.0 41.0 38 37.27160678642714 39.0 36.0 40.0 32.0 41.0 39 37.16635728542914 39.0 36.0 40.0 32.0 41.0 40 37.03945109780439 39.0 36.0 40.0 31.0 41.0 41 36.90387724550898 39.0 36.0 40.0 31.0 41.0 42 36.8350249500998 39.0 36.0 40.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.89196254359231 1101 2 6.326874078210601 1101 3 5.292040827217718 1101 4 5.3012460133062795 1101 5 8.531623450484066 1101 6 6.799629304832756 1101 7 6.357062136314301 1101 8 6.712112556420056 1101 9 6.13679299326121 1101 10 5.438998853819079 1101 11 3.3966756692140123 1101 12 8.037080468802948 1101 13 0.15034233160432464 1101 14 0.6533539806725699 1101 15 1.5220658357227492 1101 16 0.7987469465056165 1101 17 0.1530367394106804 1101 18 0.07634933856451198 1101 19 0.17386773098433395 1101 20 0.16172343863260608 1101 21 0.48716813712231755 1101 22 1.158931391618836 1101 23 0.6465076647481425 1101 24 0.3181289129756948 1101 25 12.307183063705502 1101 26 7.2335055040268195 1101 27 2.8529982564023086 1101 28 1.2289135323542908 1101 29 0.7015168284544515 1101 30 0.24261302393499307 1101 31 0.03537305835409654 1101 32 -0.11709862951708772 1101 33 -0.02845739354698651 1101 34 -0.13941434254771679 1101 35 -0.12771460353347663 1101 36 -0.5654122391643668 1101 37 -0.06496585120634535 1101 38 -0.16149059352980544 1101 39 -0.2557644891327584 1101 40 -0.3235750605663057 1101 41 -0.2968004978722121 1101 42 -0.202015107241877 1102 1 4.272158089959198 1102 2 3.712900481277316 1102 3 2.8138567379727704 1102 4 1.9561648227993196 1102 5 4.821292658392061 1102 6 3.5709875767886494 1102 7 2.8851288546672293 1102 8 3.3261615291555486 1102 9 4.545372913147499 1102 10 3.9536217594485734 1102 11 3.9475866396236263 1102 12 5.969484526174703 1102 13 -0.2107185289719773 1102 14 1.0727772481059148 1102 15 1.7010833659807503 1102 16 0.835083467158583 1102 17 0.43416156173914544 1102 18 0.15320035290744727 1102 19 0.16651859026848115 1102 20 0.22898557413676457 1102 21 0.3068153783679506 1102 22 1.1947241296986917 1102 23 0.7168817440865567 1102 24 0.5007537137682192 1102 25 12.076915371927925 1102 26 7.367652896024065 1102 27 3.1456394245118844 1102 28 1.5896405858064995 1102 29 0.8772070554433 1102 30 0.4019197819140601 1102 31 0.13188638910312278 1102 32 0.08329870864029232 1102 33 0.09131475617088824 1102 34 0.11197472580736445 1102 35 -0.04637872895881401 1102 36 -0.030739370145973055 1102 37 -0.20751226116455967 1102 38 -0.2357572946539861 1102 39 -0.30951820408300534 1102 40 -0.24262683297445164 1102 41 -0.28562334320106686 1102 42 -0.2056277617622726 1103 1 1.5085952313259163 1103 2 0.5480223234414119 1103 3 -0.26139104502149024 1103 4 0.454879332077212 1103 5 1.7858950877832243 1103 6 0.3106663904111251 1103 7 -2.9724424993829963 1103 8 -1.083146168884543 1103 9 2.3262619510787914 1103 10 1.507332915272407 1103 11 -1.4723962205000802 1103 12 1.5158739739725995 1103 13 -0.7015337909139987 1103 14 1.2107905128797434 1103 15 1.7834851842980584 1103 16 1.170652436530375 1103 17 0.5302939113926186 1103 18 0.3572580323172403 1103 19 0.374507263045885 1103 20 0.3397427095417811 1103 21 0.46543375529618913 1103 22 1.2833670986233514 1103 23 0.8636512500109959 1103 24 0.6639737748756005 1103 25 12.262883029726567 1103 26 7.321207557325199 1103 27 3.318230526680452 1103 28 1.4095601029091185 1103 29 0.526901518518045 1103 30 0.45270598058758793 1103 31 0.13501627955695739 1103 32 0.17613349513705145 1103 33 0.28009558931792355 1103 34 0.2958557899701191 1103 35 0.024043806252450395 1103 36 -0.07422249109388446 1103 37 0.039786345288973735 1103 38 -0.010162988073759038 1103 39 -0.0483959147917048 1103 40 0.14734261333302356 1103 41 -0.0287673926831431 1103 42 0.05029667373962354 1104 1 1.4903381507158961 1104 2 1.0762669546272186 1104 3 -0.45555226506506585 1104 4 -0.6577070358032735 1104 5 1.5752446053680345 1104 6 0.2550610371587112 1104 7 -0.22328594787350298 1104 8 -0.07637368211205597 1104 9 2.0683472296346572 1104 10 1.0554748387084487 1104 11 0.8008095062350584 1104 12 2.7031382130015444 1104 13 0.4268291098018402 1104 14 1.9512169056590771 1104 15 2.005919046731922 1104 16 0.8572817549243936 1104 17 0.41064871998272423 1104 18 0.20543730402592075 1104 19 0.2857646548915085 1104 20 0.14282705439083543 1104 21 0.22548977770514966 1104 22 1.0676901611817087 1104 23 0.6215130614022186 1104 24 0.2812535756848149 1104 25 12.17305732166556 1104 26 7.108238369650135 1104 27 2.9388374361108944 1104 28 1.2878234082312474 1104 29 0.6118190410826259 1104 30 0.2737921928502658 1104 31 -0.09844777273062988 1104 32 -0.12696641149226195 1104 33 -0.16722155976393083 1104 34 0.09082311023156109 1104 35 -0.37497580159068633 1104 36 -0.3295352828772664 1104 37 -0.35542996345674993 1104 38 -0.3289055962281324 1104 39 -0.41938564401672807 1104 40 -0.4019695559791501 1104 41 -0.37000611039244546 1104 42 -0.31693955408483987 1105 1 0.5922989350296213 1105 2 0.9421493075683962 1105 3 -2.429983637614086 1105 4 -0.5136286044307248 1105 5 -1.794951473063339 1105 6 -0.5495664138216796 1105 7 -1.3839133988538954 1105 8 -4.73370701544539 1105 9 1.6786217394385794 1105 10 0.9433964073359 1105 11 1.4958683297644697 1105 12 3.257334291432919 1105 13 0.18220165882144812 1105 14 1.6212561213453505 1105 15 1.6939190467319207 1105 16 0.7048111666891046 1105 17 0.007119308218015874 1105 18 0.1258294608886672 1105 19 0.17894112547974572 1105 20 -0.08697686717778907 1105 21 -0.2578043399419059 1105 22 0.7414940827503358 1105 23 0.4685718849316274 1105 24 0.2589790658808937 1105 25 12.016586733430266 1105 26 6.953493271610913 1105 27 2.781817828267755 1105 28 1.0819410552900663 1105 29 0.13777982539635047 1105 30 0.14814513402674123 1105 31 -0.11609483155415745 1105 32 -0.07669190168834206 1105 33 -0.07608430486197193 1105 34 -0.1799219878076599 1105 35 -0.09662286041421453 1105 36 -0.3358097926811894 1105 37 -0.5118221203194935 1105 38 -0.44106245897323504 1105 39 -0.6705228989186907 1105 40 -0.47263622264581073 1105 41 -0.277613953529702 1105 42 -0.3594493580064082 1106 1 -3.7546797441175457 1106 2 -4.380097182291198 1106 3 -1.9572077665792413 1106 4 -6.325362353780701 1106 5 -0.6480663976603225 1106 6 0.2680040489968327 1106 7 -2.0292196911055846 1106 8 -4.210258237494269 1106 9 -0.7655134659696365 1106 10 -0.2169322458102272 1106 11 0.3551694218081529 1106 12 2.82345389621711 1106 13 0.10844606859783923 1106 14 1.7094017271695847 1106 15 1.73952279088169 1106 16 0.7479250512444864 1106 17 0.38499762335062115 1106 18 -0.1184867117582371 1106 19 0.04700352797584628 1106 20 -0.01557801122355329 1106 21 -0.045560970207112916 1106 22 0.6872808742219974 1106 23 0.6198667367256974 1106 24 0.09252456770096984 1106 25 11.894433847314822 1106 26 6.752991971558917 1106 27 2.626968114279194 1106 28 1.2098349710466962 1106 29 0.4229633927390424 1106 30 0.2992028563356328 1106 31 -0.30604802968208134 1106 32 -0.24513287932744987 1106 33 -0.49330947386873447 1106 34 -0.44539052654920397 1106 35 -0.7514372129883142 1106 36 -0.9012908119219531 1106 37 -0.6233000194354545 1106 38 -0.5642657871063577 1106 39 -0.8865270590850969 1106 40 -0.7645592595672923 1106 41 -0.6561766160362055 1106 42 -1.2600505020521666 1107 1 2.923410046140731 1107 2 2.1599270853461725 1107 3 1.2015719179414717 1107 4 -3.9094063822085054 1107 5 -23.10828480639667 1107 6 -17.660233080488347 1107 7 -8.049691176631676 1107 8 -5.929707015445388 1107 9 1.259066183883025 1107 10 1.9827297406692317 1107 11 2.2667572186533604 1107 12 4.233334291432918 1107 13 0.3482016588214485 1107 14 2.0137005657897973 1107 15 2.057030157843034 1107 16 1.1119222778002111 1107 17 0.18267486377357045 1107 18 0.13027390533311234 1107 19 0.331385569924187 1107 20 0.2296897994888738 1107 21 -0.10758211771968007 1107 22 0.7674940827503391 1107 23 0.6041274404871828 1107 24 0.14386795476978165 1107 25 11.944364511208043 1107 26 7.152159938277581 1107 27 2.2753733838233074 1107 28 1.0563854997345103 1107 29 0.7837798253963513 1107 30 0.4450340229156282 1107 31 -0.3927614982208212 1107 32 -0.35402523502167327 1107 33 -0.38630652708419433 1107 34 -0.44858865447432095 1107 35 -1.1148450826364353 1107 36 -0.37025423712562855 1107 37 -0.5424887869861621 1107 38 -0.4199513478621242 1107 39 -0.40474512114091254 1107 40 -0.23863622264581608 1107 41 -0.12294728686303813 1107 42 -0.614782691339741 1113 1 -13.800521577790892 1113 2 -8.83323530781622 1113 3 0.6972984136679656 1113 4 -1.1098337326358525 1113 5 0.8917151936033285 1113 6 -17.660233080488347 1113 7 -8.269349296289793 1113 8 0.9036263178879445 1113 9 -20.718711593894753 1113 10 -21.333936925997435 1113 11 -10.479823977927838 1113 12 -21.83333237523375 1113 13 0.2546119152317061 1113 14 2.3513074033966355 1113 15 1.7997652005780758 1113 16 0.9777342436121828 1113 17 -1.9574960763973692 1113 18 -0.4705807955215846 1113 19 -0.12844348990486765 1113 20 -0.7305666107675393 1113 21 -0.12980433994190577 1113 22 0.22262228787854355 1113 23 -0.9052742689145248 1113 24 -0.28861067770885285 1113 25 12.101202118045656 1113 26 6.772672758790403 1113 27 2.99802295647288 1113 28 -0.13805894470993252 1113 29 -0.14057914896262247 1113 30 0.7022989801805863 1113 31 0.5239051684458431 1113 32 -0.2463329273293695 1113 33 1.0077105669329 1113 34 -0.5524348083204771 1113 35 0.4535309857396328 1113 36 1.0729081560367604 1113 37 -0.34248878698615925 1113 38 0.621501643590868 1113 39 2.037989921594132 1113 40 1.747261213251619 1113 41 1.8462834823677312 1113 42 2.1018839753269276 1114 1 -9.287701064970378 1114 2 -1.4905779651588773 1114 3 -1.1873169709474176 1114 4 -6.421022543824666 1114 5 -1.2749514730633393 1114 6 1.1124941922389269 1114 7 0.3402078132673161 1114 8 1.9339293481909756 1114 9 -5.627802502985663 1114 10 0.5600024679419597 1114 11 0.7521107540068961 1114 12 -21.83333237523375 1114 13 0.013353173972966204 1114 14 -27.26407721198798 1114 15 -13.71771731690444 1114 16 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1115 11 -8.314988813092672 1115 12 -21.83333237523375 1115 13 0.2839159445357353 1115 14 1.2121132642024932 1115 15 -27.354080953268078 1115 16 -15.099188833310897 1115 17 -3.62049973940103 1115 18 -1.45226577720657 1115 19 -1.5606779221393055 1115 20 -0.12983401003493356 1115 21 -0.22504243518000067 1115 22 0.9222559875122442 1115 23 0.010476646836387715 1115 24 -0.49740188650005734 1115 25 11.811824828668364 1115 26 6.61882660494425 1115 27 3.1079130663629897 1115 28 1.290512483861498 1115 29 0.9766369682534943 1115 30 -1.7152834374018298 1115 31 0.5715242160648941 1115 32 -0.7921204731169169 1115 33 -0.34027478105245024 1115 34 -1.3985886544743238 1115 35 0.3216628538715014 1115 36 -0.6377145545859477 1115 37 0.5146540701566948 1115 38 -0.2905862684970444 1115 39 -0.40157051796630583 1115 40 -0.021969555979147515 1115 41 1.2675289036131545 1115 42 1.4828363562793072 1201 1 7.718819840284706 1201 2 7.206775314472885 1201 3 6.792544939294242 1201 4 5.959299665611471 1201 5 9.43256674711311 1201 6 7.153345745748709 1201 7 6.894676551280359 1201 8 7.282222405344793 1201 9 6.709366656968307 1201 10 6.181598171816145 1201 11 5.952149112273101 1201 12 9.397967969990649 1201 13 0.4569665226496049 1201 14 2.0684889560212234 1201 15 2.0947107613464233 1201 16 1.02163970523916 1201 17 0.496164187389482 1201 18 0.42948116783151136 1201 19 0.3580573510263534 1201 20 0.2209249356607188 1201 21 0.09229002139296227 1201 22 1.134103212017692 1201 23 0.7341875351042404 1201 24 0.463801467108361 1201 25 -4.450271724567429 1201 26 -2.6182274802111003 1201 27 -0.7120770700828416 1201 28 -0.3359875983347891 1201 29 -0.06533793448090108 1201 30 -0.09897569451180743 1201 31 -0.10670472798683761 1201 32 -0.10178127644860524 1201 33 -0.1153309485136802 1201 34 -0.1224091377884804 1201 35 0.17009290483319717 1201 36 -0.2962163903029733 1201 37 -0.23661997225658382 1201 38 0.053651771943528104 1201 39 -0.4599298801231342 1201 40 -0.18882801397684545 1201 41 -0.21523727535555537 1201 42 -0.3676212030390076 1202 1 5.202518495907864 1202 2 4.4971193674486365 1202 3 4.491126142825038 1202 4 3.5133813756091925 1202 5 5.580337948094346 1202 6 4.392461530290095 1202 7 3.1159109524435102 1202 8 3.683267036450818 1202 9 4.811827328260936 1202 10 4.446901397355859 1202 11 4.223077910602793 1202 12 6.4205598403351125 1202 13 -0.2672674030547988 1202 14 1.66286889579645 1202 15 1.8483142563127615 1202 16 1.0828470948328146 1202 17 0.5277181106132289 1202 18 0.3495021155792841 1202 19 0.3228692691923172 1202 20 0.23737443022739768 1202 21 0.3312735043694701 1202 22 1.3344601506146105 1202 23 1.0294102082849221 1202 24 0.5368153932262061 1202 25 -3.4929941048930857 1202 26 -1.867400939965929 1202 27 -0.5952520320116861 1202 28 -0.054226609380592095 1202 29 -0.035282050851151325 1202 30 0.29925890648183184 1202 31 0.17061175527219063 1202 32 0.2615436272537721 1202 33 0.1710454356569855 1202 34 0.039734698819088976 1202 35 0.1702513910827932 1202 36 0.10902054664017413 1202 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2215 42 1.018550641993592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 9.0 25 167.0 26 1092.0 27 3742.0 28 8204.0 29 14085.0 30 20535.0 31 26172.0 32 31048.0 33 34240.0 34 27996.0 35 20168.0 36 8208.0 37 4575.0 38 158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.8709394028132604 1.504074481727944 0.4978239137239854 97.1271622017348 2 2.0028554766836253 1.4986225341350117 95.79068551549398 0.7078364736873857 3 3.152195608782435 91.2619760479042 0.7994011976047904 4.786427145708583 4 0.9555888223552893 2.213073852295409 0.8632734530938124 95.9680638722555 5 92.81168253968254 4.387047619047619 1.7168253968253968 1.0844444444444445 6 2.7081124414905995 87.9091778944374 2.8720680648581554 6.51064159921384 7 93.53636263834969 3.0758790007884307 1.6896376211339432 1.6981207397279412 8 2.384375628393324 2.6704202694550574 93.6185401166298 1.3266639855218179 9 29.70612232639393 17.1576307582955 23.3875771485206 29.74866976678997 10 24.26419490148436 15.106564876336748 22.979316964888337 37.64992325729056 11 28.86574609528216 19.103700053303296 27.29933320594181 24.73122064547274 12 27.337240662337464 18.46639847724659 32.9792630315449 21.217097828871044 13 27.84366576819407 27.522212239193372 23.368773085754217 21.265348906858343 14 24.853387868034044 27.01222018385412 25.738574278467812 22.39581766964402 15 21.260452152369155 25.591664252390135 33.9413980159642 19.206485579276517 16 22.55938123752495 26.486526946107787 32.22255489021956 18.731536926147704 17 18.737272908764226 28.650928329007787 32.45458175284488 20.15721700938311 18 20.977544910179642 29.178642714570856 24.785928143712574 25.057884231536924 19 20.97355289421158 29.65369261477046 26.351796407185628 23.020958083832337 20 19.555389221556887 31.584830339321357 25.831337325349303 23.028443113772454 21 17.693113772455092 31.465069860279442 26.55439121756487 24.2874251497006 22 18.892647517794757 30.434322993747926 29.064101402438407 21.608928086018906 23 20.669660678642714 29.62674650698603 28.858782435129744 20.844810379241515 24 19.37075848303393 29.152694610778447 31.187125748502993 20.289421157684632 25 17.280586569917038 30.678031711815652 30.050322962603133 21.99105875566418 26 19.65578670552248 31.43029655836606 28.25513101362781 20.658785722483643 27 20.966776115530095 32.01179652491542 29.374544656134294 17.646882703420193 28 18.420934445126424 35.27098709062511 29.391657559743905 16.91642090450456 29 18.481536926147704 37.13922155688623 27.572854291417165 16.806387225548903 30 20.167664670658684 37.086327345309385 26.745508982035926 16.00049900199601 31 17.942115768463076 37.591816367265466 27.469560878243517 16.996506986027942 32 19.567365269461078 37.843313373253494 26.21906187624751 16.370259481037923 33 18.285928143712574 39.377245508982035 26.813872255489024 15.522954091816368 34 19.293912175648703 39.8438123752495 24.971556886227546 15.890718562874252 35 19.708582834331338 38.85279441117764 26.602295409181636 14.83632734530938 36 20.829840319361278 38.36926147704591 26.26447105788423 14.536427145708583 37 19.71606786427146 38.69061876247505 26.07684630738523 15.516467065868264 38 18.491516966067863 38.95508982035928 26.599301397205586 15.954091816367267 39 18.722554890219563 39.115269461077844 25.79441117764471 16.367764471057882 40 19.73802395209581 38.22455089820359 26.726546906187625 15.310878243512974 41 20.57884231536926 39.28642714570858 24.737025948103792 15.397704590818364 42 18.35828343313373 40.2060878243513 25.783932135728545 15.651696606786428 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.0 7 4.0 8 12.0 9 20.0 10 20.0 11 49.0 12 78.0 13 247.0 14 416.0 15 683.0 16 950.0 17 950.0 18 1169.5 19 1389.0 20 1842.5 21 2296.0 22 2296.0 23 2806.5 24 3317.0 25 4043.5 26 4770.0 27 5443.0 28 6116.0 29 6116.0 30 7730.0 31 9344.0 32 12137.5 33 14931.0 34 14931.0 35 16926.0 36 18921.0 37 20137.5 38 21354.0 39 20413.0 40 19472.0 41 19472.0 42 18404.0 43 17336.0 44 16423.0 45 15510.0 46 14843.0 47 14176.0 48 14176.0 49 12734.5 50 11293.0 51 10852.5 52 10412.0 53 10412.0 54 9428.0 55 8444.0 56 7648.5 57 6853.0 58 5857.5 59 4862.0 60 4862.0 61 3856.0 62 2850.0 63 2262.5 64 1675.0 65 1519.0 66 1363.0 67 1363.0 68 1332.5 69 1302.0 70 902.0 71 502.0 72 502.0 73 366.0 74 230.0 75 181.5 76 133.0 77 96.0 78 59.0 79 59.0 80 35.5 81 12.0 82 10.0 83 8.0 84 8.0 85 4.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 1.1666666666666667 2 0.7405189620758483 3 0.0 4 0.0 5 1.7589820359281436 6 3.5204590818363273 7 9.980039920159682E-4 8 0.7385229540918163 9 0.31087824351297405 10 0.19011976047904192 11 0.7674650698602794 12 0.6422155688622755 13 0.029940119760479042 14 0.4461077844311377 15 0.10079840319361277 16 0.0 17 0.01996007984031936 18 0.0 19 0.0 20 0.0 21 0.0 22 0.8712574850299402 23 0.0 24 0.0 25 34.256986027944116 26 4.990019960079841E-4 27 9.980039920159682E-4 28 0.0014970059880239522 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 200400.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.225403459942434 #Duplication Level Percentage of deduplicated Percentage of total 1 77.38699933277198 43.511152600394006 2 10.955685032461322 12.319756222603806 3 4.808396220318661 8.110620524480371 4 2.461353677438612 5.535624142863959 5 1.3253693920039402 3.725971439944007 6 0.7576137959650814 2.555828480697313 7 0.4668931768516498 1.8375880068824786 8 0.3245328724816729 1.4597593353036886 9 0.2259780492031029 1.1435136290581664 >10 1.1507952750715957 12.027878157887313 >50 0.09357677730399379 3.5240132340080095 >100 0.04280639812842269 4.248294225876873 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTACGAGTATTCGGTGTCTGATCCCCGCAGCGTATTGCAGAC 458 0.22854291417165667 No Hit CTACGAGTGCCAAGGTTGGCTAGCAGGTACAATCCGAACGCG 337 0.16816367265469062 No Hit CTACGAGTATTCGGTGTCTGATCCNCGCAGCGTATTGCAGAC 318 0.15868263473053892 No Hit CTACGAGTTGCTGTGAAACCCCGCCTCTACTAAAAATACAAA 291 0.14520958083832333 No Hit CTACGAGTCGAGAGGTTCACCACGTCCGCTTGTGCGAACTGG 290 0.14471057884231536 No Hit CTCCGAGTCCTTGCGACGGCGTCATCGTCATCAAGGCTCAGA 284 0.14171656686626746 No Hit CTACGAGTCATCTTGGTACAGGACTCATTACAATTGCTTTAG 267 0.13323353293413173 No Hit CTACGAGTCCCTTACGAATCCGCCACCCCGGCCGACGCCTCC 250 0.124750499001996 No Hit CTACGAGTGCAGGTTGGAAAATGAGAGAAGAAAAAGAAAGGA 250 0.124750499001996 No Hit CTACGAGTCGGAGGTGGAAACAAGAGTGAAACTCTGTCTCAA 242 0.12075848303393215 No Hit CTACGAGTGCCAAGGTTGGCTAGCNGGTACAATCCGAACGCG 229 0.11427145708582834 No Hit CTACGAGTCGAGAGGTTCACCACGNCCGCTTGTGCGAACTGG 209 0.10429141716566867 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 4.99001996007984E-4 0.0 0.0 10 0.0 0.0 0.006487025948103792 0.0 0.0 11 0.0 0.0 0.006986027944111776 0.0 0.0 12 0.0 0.0 0.013972055888223553 0.0 0.0 13 0.0 0.0 0.030439121756487025 0.0 0.0 14 0.0 0.0 0.059880239520958084 0.0 0.0 15 0.0 0.0 0.08632734530938124 0.0 0.0 16 0.0 0.0 0.1062874251497006 0.0 0.0 17 0.0 0.0 0.12275449101796407 0.0 0.0 18 0.0 0.0 0.1412175648702595 0.0 0.0 19 0.0 0.0 0.21307385229540918 0.0 0.0 20 0.0 0.0 0.24301397205588823 0.0 0.0 21 0.0 0.0 0.27395209580838326 0.0 0.0 22 0.0 0.0 0.4565868263473054 0.0 0.0 23 0.0 0.0 0.5424151696606786 0.0 0.0 24 0.0 0.0 0.6202594810379242 0.0 0.0 25 0.0 0.0 0.6796407185628742 0.0 0.0 26 0.0 0.0 0.7430139720558883 0.0 0.0 27 0.0 0.0 0.9615768463073853 0.0 0.0 28 0.0 0.0 1.0773453093812375 0.0 0.0 29 0.0 0.0 1.2280439121756488 0.0 0.0 30 0.0 0.0 1.3642714570858283 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTGCC 20 3.7340063E-4 51.131927 19 TCACCTC 15 0.007701743 51.131924 20 CACCTCT 15 0.007701743 51.131924 21 TACCGTG 15 0.008107848 50.471905 23 GCTAGCA 95 0.0 43.058464 19 ACCACGT 95 0.0 43.058464 19 CCACGTC 95 0.0 43.058464 20 CAGGTAC 95 0.0 42.50266 24 AGGTACA 95 0.0 42.50266 25 GCAGGTA 95 0.0 42.50266 23 ACGTCCG 100 0.0 40.921272 22 CTGGATA 25 0.0011190595 40.921272 22 CATGGTG 50 1.564149E-8 40.905537 20 CACGTCC 105 0.0 38.957653 21 ATCCCCG 80 0.0 38.348946 21 ATGGTGG 55 3.6243364E-8 37.18685 21 CACCTAT 35 1.3250517E-4 36.5228 19 ACCTATA 35 1.3250517E-4 36.5228 20 AGCAGGT 115 0.0 35.583717 22 CGTCCGC 115 0.0 35.11089 23 >>END_MODULE