FastQCFastQC Report
Thu 26 May 2016
SRR2099048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892744
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGA451.3019868E-534.65508319
TGCTCAC330450.034.513293
GCTCACG103900.034.4895064
GATGCTC1431500.034.3378681
GCTCACC75000.034.3233954
TCACGCG9650.034.3022276
GCTCCCG14700.034.2676244
TGCTCAA270350.034.1996233
CTCACGC30800.034.1208655
TGCTCAG454450.034.1163063
GCTCCGG19300.034.110274
GCTCAAG84150.034.100384
CTCACGT29650.034.09685
GCTCAAC75350.034.0948034
ATGCTCG39800.034.0592582
TCAACCG8450.034.0191356
CTCACCG23100.033.989275
TGCTCCG67800.033.970743
ATGCTCC171150.033.8384972
ATGCTCA1269850.033.781712
Produced by FastQC (version 0.11.5)