##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099048_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1892744 Sequences flagged as poor quality 0 Sequence length 42 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.23666010828723 30.0 26.0 31.0 18.0 35.0 2 24.35735366219626 27.0 18.0 31.0 8.0 33.0 3 27.34085909135097 29.0 25.0 31.0 17.0 35.0 4 27.998166154535426 30.0 26.0 31.0 17.0 35.0 5 28.90312636045868 30.0 27.0 32.0 19.0 38.0 6 27.95956135642221 30.0 27.0 32.0 13.0 39.0 7 29.610756658058353 30.0 28.0 32.0 19.0 39.0 8 23.653626163918627 26.0 17.0 30.0 8.0 34.0 9 26.156316437933498 27.0 26.0 28.0 22.0 31.0 10 26.192831677184024 27.0 26.0 27.0 22.0 31.0 11 25.606948958760402 27.0 26.0 27.0 18.0 31.0 12 27.974250611810156 30.0 26.0 31.0 18.0 35.0 13 31.108383912457256 31.0 31.0 33.0 30.0 34.0 14 31.07444482719269 31.0 31.0 34.0 30.0 34.0 15 31.316424196827462 31.0 31.0 34.0 30.0 34.0 16 35.231970620432556 35.0 35.0 37.0 33.0 37.0 17 35.273356565917 35.0 35.0 37.0 33.0 37.0 18 34.800194321049226 35.0 35.0 37.0 32.0 37.0 19 34.688389977725464 35.0 35.0 37.0 32.0 37.0 20 34.60685914207098 35.0 35.0 37.0 32.0 37.0 21 35.96831478530641 37.0 35.0 39.0 32.0 39.0 22 35.80327926016408 37.0 35.0 39.0 32.0 39.0 23 36.08284110265308 37.0 35.0 39.0 32.0 39.0 24 36.230471210052706 37.0 35.0 39.0 32.0 39.0 25 22.196027566326983 32.0 2.0 37.0 2.0 39.0 26 29.186479523908147 34.0 17.0 38.0 17.0 40.0 27 34.01275396989767 35.0 32.0 39.0 27.0 40.0 28 36.24833099457719 37.0 34.0 39.0 32.0 40.0 29 37.19504169607723 38.0 36.0 40.0 33.0 40.0 30 37.7183649769858 39.0 37.0 40.0 33.0 41.0 31 38.16107883580664 39.0 37.0 40.0 34.0 41.0 32 38.307811832979 40.0 38.0 40.0 34.0 41.0 33 38.33293990101144 40.0 38.0 40.0 34.0 41.0 34 38.33107858220657 40.0 38.0 40.0 34.0 41.0 35 38.29177532724975 40.0 38.0 40.0 34.0 41.0 36 38.30108297794102 40.0 38.0 40.0 34.0 41.0 37 38.248044109504505 40.0 38.0 40.0 34.0 41.0 38 38.13555715934115 40.0 37.0 40.0 34.0 41.0 39 38.066604886873236 40.0 37.0 40.0 34.0 41.0 40 38.055799410802514 40.0 37.0 40.0 34.0 41.0 41 37.95870545620538 39.0 37.0 40.0 34.0 41.0 42 37.91666860388938 39.0 37.0 40.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.0663488983651455 1101 2 6.0426151217212904 1101 3 5.65104477385497 1101 4 5.72335749472883 1101 5 8.420186980924719 1101 6 9.498504643549978 1101 7 7.292593047848751 1101 8 6.087312009058717 1101 9 6.235407645436013 1101 10 5.7894192160065145 1101 11 3.6760896064372517 1101 12 8.192397074685154 1101 13 0.17416000794976583 1101 14 1.095748454371094 1101 15 1.646205358528924 1101 16 0.8910545818413382 1101 17 0.2723384100621331 1101 18 0.06271302509438925 1101 19 0.09867314207613731 1101 20 -0.007620301634268856 1101 21 -0.02770514307298555 1101 22 0.9268497189942693 1101 23 0.39351904650840197 1101 24 0.1990950782671277 1101 25 11.949516739801957 1101 26 7.003789294933341 1101 27 3.086872512922959 1101 28 1.2617180200617995 1101 29 0.6120152937272323 1101 30 0.3258582949950224 1101 31 0.10928635624890859 1101 32 0.05413720953207246 1101 33 0.06261501876952735 1101 34 0.14065510090470923 1101 35 -0.023179707075819067 1101 36 4.6791533847567734E-6 1101 37 -0.038638444772033154 1101 38 0.03190095265002668 1101 39 -5.358302626987665E-4 1101 40 -0.04914830086021027 1101 41 -0.11654225694302056 1101 42 0.014492634531428905 1102 1 4.421090785437713 1102 2 3.1026880639383663 1102 3 2.2075367295102204 1102 4 1.3570908180437158 1102 5 4.408534355216702 1102 6 5.411837128845047 1102 7 3.6572322727031157 1102 8 3.313586877843914 1102 9 4.458002561845831 1102 10 3.643662807056071 1102 11 3.948829996289433 1102 12 5.750524802863755 1102 13 -0.046215300672287185 1102 14 1.3998399532455323 1102 15 1.7457973672348892 1102 16 0.9464654147779683 1102 17 0.4879416754044783 1102 18 0.21095580828033889 1102 19 0.16215681183440722 1102 20 0.021837975891592976 1102 21 -0.03383616977585291 1102 22 0.894014311875388 1102 23 0.415117610407286 1102 24 0.20510873277583386 1102 25 11.903280668137093 1102 26 6.908303926240933 1102 27 2.945306707338709 1102 28 1.2820333692686603 1102 29 0.6518566609834409 1102 30 0.4255345822313785 1102 31 0.22785786478543457 1102 32 0.0884152482490066 1102 33 0.18827922436022249 1102 34 0.22899608339191957 1102 35 0.09401321398148355 1102 36 0.06628630787096768 1102 37 -0.01681931948358084 1102 38 0.031227922082322834 1102 39 0.0540852302675674 1102 40 -0.039781470267492125 1102 41 0.03733521102483195 1102 42 6.449849001555208E-4 1103 1 1.8095274357605362 1103 2 -0.8066217020882576 1103 3 -0.2598775860125606 1103 4 0.274912151933826 1103 5 2.130042411087281 1103 6 2.008153676999207 1103 7 -0.018607731241534253 1103 8 -1.0918745176681135 1103 9 2.291549526904845 1103 10 1.4520060690643781 1103 11 -1.3899426365903267 1103 12 2.0076581955325956 1103 13 -0.6028634331620495 1103 14 1.374362678193851 1103 15 1.6910020915625417 1103 16 0.9454725594734512 1103 17 0.4417247968569882 1103 18 0.1425913394123839 1103 19 0.15957366152863273 1103 20 0.07245640443900214 1103 21 0.10698826408483342 1103 22 0.9311053566665706 1103 23 0.5026520181493268 1103 24 0.31831724548495544 1103 25 12.035898198019318 1103 26 7.111769471531858 1103 27 3.08944217080019 1103 28 1.298515323319684 1103 29 0.5324365019422359 1103 30 0.3001764951738153 1103 31 0.1884777257542396 1103 32 0.12923289310536745 1103 33 0.1368997396485767 1103 34 0.15692399879741714 1103 35 0.05750687950391864 1103 36 0.06580189631743139 1103 37 -0.025582402399713544 1103 38 -0.03323762452544088 1103 39 -0.045230916545236255 1103 40 0.09578905702598917 1103 41 0.1355054938848923 1103 42 -0.007329205470838929 1104 1 1.4388547922182866 1104 2 0.8586745687772392 1104 3 -0.3375474372230052 1104 4 -0.4568547019533611 1104 5 1.1414464249515959 1104 6 0.971455714557564 1104 7 -0.8100589550345134 1104 8 0.22878960907966217 1104 9 2.1861774144873287 1104 10 1.147594235464446 1104 11 0.8005904194090654 1104 12 2.856227132001333 1104 13 0.29914922651109066 1104 14 1.9751341575159884 1104 15 1.8679656260523565 1104 16 0.8852755060981394 1104 17 0.28958434802675725 1104 18 0.06830541509291521 1104 19 0.02564779465711098 1104 20 -0.11839393660144992 1104 21 -0.11376332459339267 1104 22 0.8017866026553548 1104 23 0.35794572168727257 1104 24 0.20835790638224694 1104 25 11.911891957484336 1104 26 6.786806616598078 1104 27 2.8908338381055216 1104 28 1.191906022682204 1104 29 0.3931355955869762 1104 30 0.19225980886302807 1104 31 0.043574578502621364 1104 32 -0.045542678002121306 1104 33 -0.016356420429318064 1104 34 -0.013951275186784073 1104 35 -0.10358908857035232 1104 36 -0.07291147411143584 1104 37 -0.13775182546155662 1104 38 -0.10799530552333181 1104 39 -0.1681096779836082 1104 40 -0.2117481831885044 1104 41 -0.14197971381726404 1104 42 -0.1061475570290682 1105 1 0.6801476646128073 1105 2 0.5831498480464568 1105 3 -2.2823200390817497 1105 4 -1.2349838102713093 1105 5 -0.5606159612163921 1105 6 -1.900429510676041 1105 7 -1.6290888875885976 1105 8 -2.0633957077667446 1105 9 1.6398911337637472 1105 10 0.7088913399842021 1105 11 1.1278350598249531 1105 12 3.049999965885128 1105 13 0.30431866980299915 1105 14 1.8319453194561817 1105 15 1.701997164566496 1105 16 0.8449937458601511 1105 17 0.2952697697949134 1105 18 -0.04926176805902571 1105 19 -0.05001124940994828 1105 20 -0.13276568157602497 1105 21 -0.1943051409213581 1105 22 0.7656465153437182 1105 23 0.39902447586770506 1105 24 0.10561043300582185 1105 25 11.987871312175578 1105 26 6.618177358774908 1105 27 2.815317137993901 1105 28 1.14474749962487 1105 29 0.4337855184032051 1105 30 0.11316813076220456 1105 31 0.10338313102398189 1105 32 -0.12405087809365511 1105 33 -0.08583371183097199 1105 34 -0.11653470045976633 1105 35 -0.20479865229200556 1105 36 -0.18754911142506359 1105 37 -0.21610853721041678 1105 38 -0.11869438548045963 1105 39 -0.18032022599388142 1105 40 -0.25890931368155634 1105 41 -0.12687549975311185 1105 42 -0.14333531935638177 1106 1 -4.803387087504522 1106 2 -4.159252969018073 1106 3 -1.3110465775131281 1106 4 -6.887061157079295 1106 5 -0.1456398740689302 1106 6 1.0501508342087753 1106 7 -0.9846706912668637 1106 8 -2.9147549347530664 1106 9 -0.44280915525191133 1106 10 0.3371386516065904 1106 11 0.831505483889142 1106 12 3.024956735448683 1106 13 0.21916597305775554 1106 14 1.9182711188763761 1106 15 1.741603171193546 1106 16 0.8443476652211572 1106 17 0.209485732735736 1106 18 -0.04145579617702566 1106 19 -0.016189878321434037 1106 20 -0.13181385002136636 1106 21 -0.28208651973469046 1106 22 0.7189959909954453 1106 23 0.3119933297270805 1106 24 0.25334858276807637 1106 25 11.998308321252793 1106 26 6.954613031802353 1106 27 2.9324448969906953 1106 28 1.2267618093772867 1106 29 0.4860467867860123 1106 30 0.2621706235950043 1106 31 -0.04014061572282657 1106 32 -0.0817293821834042 1106 33 -0.10561683510081821 1106 34 -0.07457304026693379 1106 35 -0.1893235552676984 1106 36 -0.2095629279861484 1106 37 -0.11493014242627453 1106 38 -0.08464077114998503 1106 39 -0.12305023948459848 1106 40 -0.08842204896221517 1106 41 -0.03588571740287705 1106 42 -0.09515457193877808 1107 1 2.7399837286413415 1107 2 1.577747652556205 1107 3 1.2560099857461822 1107 4 -3.910984232553478 1107 5 -24.51605293843012 1107 6 -23.618052816127438 1107 7 -11.246159455840896 1107 8 -6.814653222598462 1107 9 1.019171218069367 1107 10 1.4234451819036273 1107 11 2.0955144496272133 1107 12 4.133656891784032 1107 13 0.3410981272835194 1107 14 1.9187212893384142 1107 15 1.6828794703779657 1107 16 0.8643604734184009 1107 17 0.3573544863851339 1107 18 0.08095289832863273 1107 19 0.20141385865846928 1107 20 0.32570248984791306 1107 21 -0.043132648080359104 1107 22 1.0771282544675955 1107 23 0.5382275313808478 1107 24 0.5260049652057788 1107 25 12.132560831094388 1107 26 7.115047003661992 1107 27 3.1120459593176406 1107 28 1.3890115315524199 1107 29 0.888534967456934 1107 30 0.2593848406939472 1107 31 0.2465369755826643 1107 32 0.06049246332713665 1107 33 -0.05066590179762187 1107 34 -0.025979116626388077 1107 35 -0.36278610070854 1107 36 -0.0077132736191174445 1107 37 0.019450449387782953 1107 38 -0.043085705319619194 1107 39 0.035820378887628124 1107 40 -0.35543717393631624 1107 41 -0.23039326105434554 1107 42 -0.20904204815885663 1113 1 -10.630904806953076 1113 2 -2.3246370851226494 1113 3 0.24890876688025543 1113 4 1.3757318953391184 1113 5 1.1055686831914997 1113 6 -23.618052816127438 1113 7 -12.525315082055346 1113 8 -0.5379706576806029 1113 9 -21.1834431610156 1113 10 -21.785051550122517 1113 11 -11.05914020502744 1113 12 -22.194447683379366 1113 13 -0.10488314810952204 1113 14 1.6070449070737958 1113 15 1.2757075926178523 1113 16 0.17727338633131495 1113 17 -0.23907723945836068 1113 18 -0.44227621313577714 1113 19 0.4994707390682933 1113 20 0.21142939322187715 1113 21 0.47009822985639715 1113 22 0.41765113028458956 1113 23 0.8966624368745713 1113 24 0.7176318862444617 1113 25 12.58553733701207 1113 26 7.464808529894107 1113 27 2.7465451644050844 1113 28 1.1190768910295432 1113 29 0.9742442061073504 1113 30 0.8062316875407944 1113 31 -0.09944429981037217 1113 32 0.46144635839867476 1113 33 0.2554107631025673 1113 34 0.025725529195902652 1113 35 0.46950501393551036 1113 36 0.511965228412322 1113 37 0.369123652002159 1113 38 0.5597759798950079 1113 39 -0.1272250194518847 1113 40 0.38697582730021196 1113 41 -0.15079601675121523 1113 42 -0.2476041396621227 1114 1 -9.129215617763887 1114 2 -4.3360391121496775 1114 3 -0.3795751858224463 1114 4 -9.102625368174394 1114 5 -1.1293341884301213 1114 6 -0.12391219112743812 1114 7 -1.193231044893185 1114 8 1.7118709808329093 1114 9 -4.6443806610155995 1114 10 0.7461984498774825 1114 11 1.308839102404992 1114 12 -22.194447683379366 1114 13 -0.29660612108249396 1114 14 -27.595657795628906 1114 15 -13.964051697922688 1114 16 -6.119971123803822 1114 17 -2.7485789286475466 1114 18 -1.348262277324963 1114 19 -0.558647926472247 1114 20 -0.28962635002136494 1114 21 0.031014614991534017 1114 22 0.9606198802845896 1114 23 0.503398078766466 1114 24 -0.038808147539320714 1114 25 12.40680000593099 1114 26 7.004293327191405 1114 27 2.8189163637294072 1114 28 1.0761081410295432 1114 29 0.42779150340464867 1114 30 -0.2717349509727214 1114 31 -0.36343289778334764 1114 32 -0.33907095579051116 1114 33 -0.6827226828433766 1114 34 -0.17354600796625874 1114 35 -0.35149371917259487 1114 36 -0.49004068374983945 1114 37 -0.472673222997841 1114 38 -0.46872908767255694 1114 39 -0.5884264552626988 1114 40 -0.6168776355376266 1114 41 -0.5432685674268924 1114 42 -0.2026822646621227 1115 1 -9.090364266412536 1115 2 -2.2503127607983267 1115 3 0.14080065877214665 1115 4 0.341948111555336 1115 5 -2.576863749240932 1115 6 2.530595832521211 1115 7 1.042252485512222 1115 8 5.185002315292369 1115 9 -21.1834431610156 1115 10 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1201 5 9.236633905300579 1201 6 10.061625997955332 1201 7 7.989410390792425 1201 8 6.936382880539519 1201 9 6.919706931634185 1201 10 6.445214045924423 1201 11 6.354762270641558 1201 12 9.51994391637357 1201 13 0.23665901171553116 1201 14 1.9722236126478094 1201 15 1.8535734757956561 1201 16 1.028487849713784 1201 17 0.43850318222336426 1201 18 0.27563774776768923 1201 19 0.271283503036706 1201 20 0.16108299988598418 1201 21 0.14402274114965508 1201 22 1.1134510067515464 1201 23 0.5564975035657227 1201 24 0.2955528584829139 1201 25 -4.871393033754 1201 26 -2.9058373476078536 1201 27 -1.1830526854521892 1201 28 -0.3985883344182639 1201 29 -0.18262773686712563 1201 30 0.0313222506177695 1201 31 0.08632269015674154 1201 32 0.0981373421557521 1201 33 0.10038513834253848 1201 34 0.18845527561929032 1201 35 0.16392933325174397 1201 36 0.1333114047307049 1201 37 0.010816054719889223 1201 38 0.0886877205423886 1201 39 0.09815935194854575 1201 40 0.039008279765027964 1201 41 0.11102058675778892 1201 42 0.0651197639821035 1202 1 5.03005088357148 1202 2 4.182292393001028 1202 3 3.7285792042004466 1202 4 3.6031569548893216 1202 5 5.292417111169268 1202 6 5.982099797378858 1202 7 3.7775058617423234 1202 8 3.7286905094002485 1202 9 4.825840827284033 1202 10 4.278283054990034 1202 11 4.095408468025621 1202 12 6.2940427146875315 1202 13 -0.16904269109393866 1202 14 1.7740484233714753 1202 15 1.7970391110878694 1202 16 0.9900588921729039 1202 17 0.42020107739340773 1202 18 0.22024514072476364 1202 19 0.13742441193230803 1202 20 0.09975171019738127 1202 21 0.02345796120162902 1202 22 1.016722731454628 1202 23 0.5000512129073797 1202 24 0.23487824362817378 1202 25 -3.737372562586117 1202 26 -2.331242767492558 1202 27 -0.9380965547654441 1202 28 -0.3807959310485458 1202 29 -0.12315517725158998 1202 30 0.030731654886999138 1202 31 0.08006905559004451 1202 32 0.12454001290782202 1202 33 0.10604373105717002 1202 34 0.21368418605955952 1202 35 0.21902122224607723 1202 36 0.19649008138433288 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-0.059193456831437175 2215 39 -0.2090638676458738 2215 40 0.03511929787358525 2215 41 0.21091148514319968 2215 42 0.06547997248740955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 54.0 25 588.0 26 4461.0 27 18123.0 28 46866.0 29 93302.0 30 157795.0 31 229363.0 32 303611.0 33 351996.0 34 305982.0 35 226952.0 36 95156.0 37 55225.0 38 3269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 97.27596954021142 1.701903201276655 0.4174297679101201 0.6046974906018058 2 2.690769146915835 92.75311825704634 0.8775257855357331 3.678586810502095 3 0.40322410215010585 0.7071215124707831 98.3750047550012 0.5146496303779063 4 97.1074271005482 1.5124602164899215 0.6271318255400625 0.7529808574218172 5 0.6143948229805485 2.4493020805899395 0.3139965493703407 96.62230654705917 6 0.609489566866379 1.4171029553698293 97.18754005475382 0.7858674230099703 7 0.44290779561044186 1.737130551339105 1.1972194499024948 96.62274220314796 8 2.942435375058446 83.07533230913099 1.2882506178611983 12.693981697949368 9 33.565145899947666 20.706117181888107 21.717436585277994 24.011300332886236 10 29.281365710977987 18.301898572763456 26.63203075643431 25.78470495982425 11 26.773348471266523 19.418247944592597 25.51623719730147 28.29216638683941 12 30.535411505233323 20.06356265324942 23.85942061626983 25.541605225247434 13 31.514481440649927 28.117333440442753 20.53768578111837 19.830499337788947 14 28.50338934598166 27.060881998995473 23.718951326117086 20.716777328905785 15 20.825164176263446 26.858337827691592 32.44011928555566 19.876378710489302 16 21.547869125460178 29.340100932825568 28.954259001745612 20.15777093996864 17 20.818315124773772 31.016869889163374 27.89098643275163 20.273828553311226 18 22.52956554082327 29.697465690024643 25.38520793091934 22.387760838232744 19 22.358913830924838 28.67661976474367 27.110005367868027 21.854461036463462 20 21.910622884024463 27.485069296217556 28.072945945146305 22.531361874611676 21 21.01039548930019 27.346434594430097 28.317511507102918 23.325658409166795 22 21.52727605724237 27.168297875852055 29.8236292225939 21.480796844311673 23 21.79354418769786 28.14828629756586 28.68597126711272 21.372198247623555 24 21.10142734569493 27.663698841470374 29.887771404902093 21.34710240793261 25 18.264470752123454 30.240259948269898 30.008304085593686 21.486965214012958 26 20.74415813043267 30.00249377085627 29.15260398284793 20.10074411586313 27 21.67141377045785 29.39115025732631 29.448421938012387 19.48901403420345 28 22.014442767837895 29.951307964444666 29.385875928297594 18.648373339419837 29 22.06184902506316 29.682872114365537 29.128807050949472 19.12647180962183 30 22.325628822492636 30.184536313415865 28.414883365103787 19.074951498987712 31 20.974046146758358 30.375159028373623 28.905440989378384 19.745353835489638 32 20.950799474202533 30.3213218480682 28.91928332621844 19.80859535151082 33 21.10237834593585 30.421493873445115 29.202892731399494 19.273235049219544 34 21.731729172038055 30.62300025782673 28.493763551753432 19.15150701838178 35 21.81451902634482 30.53682906933003 28.524142726116157 19.124509178208992 36 21.394388253245026 30.944966672724888 28.554099233705138 19.106545840324944 37 22.00244724061997 30.560551242006312 28.18886230784512 19.2481392095286 38 21.520818451940674 30.390058032148037 28.65374292561487 19.43538059029642 39 21.453931434995962 30.677524271639484 28.955421335373405 18.91312295799115 40 21.38873508514622 30.59922525180373 28.543955231135325 19.468084431914722 41 21.81055652534099 29.840221392856087 29.39790061413482 18.951321467668105 42 21.936986724036636 30.324333348831118 29.009786849146003 18.728893077986246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 10.0 7 18.0 8 48.5 9 79.0 10 79.0 11 259.5 12 440.0 13 959.0 14 1478.0 15 2509.5 16 3541.0 17 3541.0 18 5253.0 19 6965.0 20 9462.5 21 11960.0 22 11960.0 23 15355.5 24 18751.0 25 24050.0 26 29349.0 27 36280.5 28 43212.0 29 43212.0 30 54233.0 31 65254.0 32 83140.5 33 101027.0 34 101027.0 35 118655.0 36 136283.0 37 146800.0 38 157317.0 39 158453.5 40 159590.0 41 159590.0 42 158386.5 43 157183.0 44 158730.0 45 160277.0 46 159654.0 47 159031.0 48 159031.0 49 156320.0 50 153609.0 51 150181.0 52 146753.0 53 146753.0 54 134881.5 55 123010.0 56 107659.0 57 92308.0 58 80608.5 59 68909.0 60 68909.0 61 57206.5 62 45504.0 63 35552.5 64 25601.0 65 18837.5 66 12074.0 67 12074.0 68 9067.0 69 6060.0 70 4629.0 71 3198.0 72 3198.0 73 2660.5 74 2123.0 75 1800.5 76 1478.0 77 884.5 78 291.0 79 291.0 80 174.0 81 57.0 82 33.5 83 10.0 84 10.0 85 6.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 1.3400650061498016 2 1.1277806190377568 3 0.0 4 0.0 5 1.7019734311666024 6 3.546068565004037 7 0.001320833667944529 8 1.128995786012266 9 0.26480073374951923 10 0.1478277041163517 11 1.1595334604151433 12 0.5384774697476257 13 0.031594341337233135 14 0.38446826406529355 15 0.07840468652918725 16 0.0 17 0.016853837602972193 18 0.0 19 0.0 20 0.0 21 0.0 22 0.7652910272070602 23 0.0 24 0.0 25 35.67682687146281 26 0.0014793337080978726 27 5.811668138955929E-4 28 0.0016906670949689972 29 3.17000080306687E-4 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 1892744.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.508968121781166 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9982763789022 38.155338157172075 2 13.799594429476794 15.044033056997188 3 7.208334403104811 11.787566105699357 4 3.8848296915083833 8.470322312519176 5 1.9556118709453483 5.329919256596841 6 1.005403282406637 3.2882097282142517 7 0.5541753623073143 2.1145269010520145 8 0.34546504741476236 1.506475460537672 9 0.2199969577966295 1.0792626443482782 >10 0.9432934153543399 8.986407557696516 >50 0.061885399059751184 2.2809006181058673 >100 0.023024664404097755 1.9267096114462694 >500 1.0909731888603068E-4 0.030328589614385247 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 2.1133338687112467E-4 0.0 0.0 0.0 0.0 9 2.1133338687112467E-4 0.0 0.0 0.0 0.0 10 2.1133338687112467E-4 0.0 0.0 0.0 0.0 11 2.1133338687112467E-4 0.0 6.340001606133741E-4 0.0 0.0 12 2.1133338687112467E-4 0.0 0.006023001525827053 0.0 0.0 13 2.1133338687112467E-4 0.0 0.016008504055487694 0.0 0.0 14 2.1133338687112467E-4 0.0 0.030643341096313077 0.0 0.0 15 2.1133338687112467E-4 0.0 0.04564801156416293 0.0 0.0 16 2.1133338687112467E-4 0.0 0.058750681550172656 0.0 0.0 17 2.1133338687112467E-4 0.0 0.06921168420029333 0.0 0.0 18 2.1133338687112467E-4 0.0 0.07502335233924926 0.0 0.0 19 2.1133338687112467E-4 0.0 0.08802035563182342 0.0 0.0 20 2.1133338687112467E-4 0.0 0.13065686643307284 0.0 0.0 21 2.1133338687112467E-4 0.0 0.16705904232162405 0.0 0.0 22 2.1133338687112467E-4 0.0 0.23991622744544427 0.0 0.0 23 2.1133338687112467E-4 0.0 0.3005160761307393 0.0 0.0 24 2.1133338687112467E-4 0.0 0.3311594172270524 0.0 0.0 25 2.1133338687112467E-4 0.0 0.3600064245349609 0.0 0.0 26 2.1133338687112467E-4 0.0 0.43376177655298337 0.0 0.0 27 2.1133338687112467E-4 0.0 0.5009657935780011 0.0 0.0 28 2.1133338687112467E-4 0.0 0.5854991483264509 0.0 0.0 29 2.1133338687112467E-4 0.0 0.6558203328078176 0.0 0.0 30 2.1133338687112467E-4 0.0 0.8320723774583356 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACGGA 45 1.3019868E-5 34.655083 19 TGCTCAC 33045 0.0 34.51329 3 GCTCACG 10390 0.0 34.489506 4 GATGCTC 143150 0.0 34.337868 1 GCTCACC 7500 0.0 34.323395 4 TCACGCG 965 0.0 34.302227 6 GCTCCCG 1470 0.0 34.267624 4 TGCTCAA 27035 0.0 34.199623 3 CTCACGC 3080 0.0 34.120865 5 TGCTCAG 45445 0.0 34.116306 3 GCTCCGG 1930 0.0 34.11027 4 GCTCAAG 8415 0.0 34.10038 4 CTCACGT 2965 0.0 34.0968 5 GCTCAAC 7535 0.0 34.094803 4 ATGCTCG 3980 0.0 34.059258 2 TCAACCG 845 0.0 34.019135 6 CTCACCG 2310 0.0 33.98927 5 TGCTCCG 6780 0.0 33.97074 3 ATGCTCC 17115 0.0 33.838497 2 ATGCTCA 126985 0.0 33.78171 2 >>END_MODULE