FastQCFastQC Report
Thu 26 May 2016
SRR2099047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694267
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT250.001181145840.4962125
CAGCGAC1350.034.806216
TAGGTCG200.002485466434.806216
CCGTGCG301.26289815E-534.806216
CAGTCGC1650.034.806216
CAATCCG200.002485466434.806216
CTTCCGT650.034.7091873
TCCCGTA200.00251973334.7091874
ACCGTTA454.8858055E-934.7091875
ACAGGCG11750.034.5614855
CTACAGG134450.034.541383
ACAGCGC6850.034.455835
TACAGCG26300.034.445244
CTACAGC117100.034.4127773
CTACCGA5500.034.3936463
ACAGCGG9350.034.337965
TTCGACA30850.034.3263631
ACAGCCG7700.034.258425
TACAGGG43550.034.1911354
CAGGCGA2750.034.173376
Produced by FastQC (version 0.11.5)