FastQCFastQC Report
Thu 26 May 2016
SRR2099046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451106
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTCTTATGCTTTCAGGGTGAAGCTCAATGCCCTACA15410.3416048556215169No Hit
GACTACGTCTTATGCTTTCAGGGTNAAGCTCAATGCCCTACA10280.22788435533998663No Hit
GACTACGTTTATATCCGGTGACTACGTTTTTTTTTTTTTTTT9470.20992848687448182No Hit
GACTACGTTTATATCCGGTGACTANGTTTTTTTTTTTTTTTT6220.13788333562399968No Hit
GACTACGTGGGTGTTGAAGGTGGGGTCTTCCTCATGGAAACA5620.12458269231621837No Hit
GACTACGTAGGGGGTGGCTCACGCCTGTAATCCCAGCACTTT4810.10662682385071356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG309.576743E-750.63823724
CGCCTGC1500.037.50916720
CCCTACG301.1938615E-535.0887571
GACAACG1450.035.0887571
CGTGGCG1500.034.8395846
CGTATAG1850.034.8395846
CGTCGTC1950.034.8395846
CCCTCGA200.00247284934.839586
CGTATGA1000.034.839586
CGTGCTA1100.034.839586
CGTTAGG2100.034.839586
CGTAGCG1550.034.839586
CGTACCA359.000778E-734.839586
CGTGTTG4250.034.839586
CGTCATA503.4197E-1034.839586
CCGTGCG750.034.7465485
CCGTAGG850.034.7465485
TACGCCG1500.034.7465484
ACGCTCG454.827598E-934.7465485
CCGTTAG359.1910806E-734.7465485
Produced by FastQC (version 0.11.5)