FastQCFastQC Report
Thu 26 May 2016
SRR2099045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302848
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGATCGAGGAAAATCACGAAAAATGAGAAATACACACTT7370.24335640321217245No Hit
TGACGACTGGGTAGTGGACATTTCTAAATTTTCCACCTTTTT7330.24203560862214707No Hit
TGACGATCGAGGAAAATCACGAAANATGAGAAATACACACTT5180.17104289940828404No Hit
TGACGATCAAAAGAGTCGTATTACCGGCCGACGATCAAAAAG5170.17071270076077769No Hit
TGACGATCTGGGTGTGACCCCACTCTCCCACTCTGAGCAAAG4990.16476912510566358No Hit
TGACGACTGGGTAGTGGACATTTCNAAATTTTCCACCTTTTT4670.15420276838546068No Hit
TGACAATCAAGCGTATCAAATGATGGCCTAGTCGGCCATCAC3810.12580568469991546No Hit
TGACGATCAAAAGAGTCGTATTACNGGCCGACGATCAAAAAG3790.1251452874049028No Hit
TGACGATCTGGGTGTGACCCCACTNTCCCACTCTGAGCAAAG3260.10764475908706678No Hit
TGACGATCGGGTGCCGACGATCGTCAACCCGATCGTCGGACT3070.10137098478444632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTC150.00792319950.77646321
GCCTCGC204.096331E-450.1945723
CTCTCCC1400.043.0239223
ACAGGCA850.041.8159121
TACCATA250.001161797140.6211719
GACAGGC950.037.41423820
CACTCTC1650.036.92833321
CTCGATA351.3831856E-436.268920
CAGGCAG1000.035.5435222
AGCCGAT301.2241175E-534.9550671
CGACGCT301.2241175E-534.9550671
TGGCGTT700.034.9550671
TGGCGGT750.034.9550671
TGACGTT4200.034.9550671
TGACGTC301.2241175E-534.9550671
TAACGCT358.757452E-734.9550671
AGACGAG800.034.9550671
TTACGCT200.002431711634.9550671
TCACGCT251.7202523E-434.9550631
TGCCGTT251.7202523E-434.9550631
Produced by FastQC (version 0.11.5)