##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099045_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302848
Sequences flagged as poor quality	0
Sequence length	42
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.234444341715978	30.0	25.0	31.0	17.0	35.0
2	27.53569117180896	30.0	26.0	31.0	16.0	35.0
3	24.456786903000847	27.0	18.0	30.0	10.0	33.0
4	27.28568456783601	29.0	25.0	31.0	17.0	35.0
5	26.961013445688927	29.0	24.0	31.0	13.0	37.0
6	21.037031778317836	23.0	15.0	28.0	7.0	32.0
7	25.871605557903635	28.0	23.0	31.0	14.0	34.0
8	27.495126267962807	29.0	25.0	31.0	16.0	37.0
9	25.59633875739645	26.0	26.0	27.0	20.0	31.0
10	25.55500118871513	27.0	26.0	27.0	18.0	31.0
11	25.148018147717668	27.0	25.0	27.0	16.0	31.0
12	27.346629332206255	30.0	25.0	31.0	17.0	35.0
13	30.890440088757398	31.0	31.0	31.0	30.0	34.0
14	30.807514000422653	31.0	31.0	33.0	28.0	34.0
15	31.068341214074387	31.0	31.0	33.0	30.0	34.0
16	34.989067122781066	35.0	35.0	37.0	32.0	37.0
17	35.03611382607777	35.0	35.0	37.0	33.0	37.0
18	34.743534710481825	35.0	35.0	37.0	32.0	37.0
19	34.639386755071854	35.0	35.0	37.0	32.0	37.0
20	34.53518926986475	35.0	35.0	37.0	32.0	37.0
21	35.85605320160609	37.0	35.0	39.0	31.0	39.0
22	35.58192228444632	37.0	35.0	39.0	30.0	39.0
23	35.779991943153	37.0	35.0	39.0	30.0	39.0
24	35.84711142223161	37.0	35.0	39.0	30.0	39.0
25	22.57125686813187	32.0	2.0	37.0	2.0	39.0
26	29.216765506128485	33.0	17.0	38.0	17.0	40.0
27	33.65980954142012	34.0	32.0	38.0	27.0	40.0
28	35.64521145921386	37.0	34.0	39.0	30.0	40.0
29	36.52526349852071	38.0	36.0	39.0	32.0	40.0
30	37.018880758664416	38.0	36.0	40.0	32.0	41.0
31	37.48175982671175	39.0	36.0	40.0	32.0	41.0
32	37.72239539306847	39.0	37.0	40.0	33.0	41.0
33	37.87505613377007	39.0	37.0	40.0	33.0	41.0
34	37.961036559594255	39.0	37.0	40.0	34.0	41.0
35	38.0089913091716	39.0	37.0	40.0	34.0	41.0
36	38.09326460798817	39.0	37.0	40.0	34.0	41.0
37	38.10963585693153	39.0	37.0	40.0	34.0	41.0
38	38.02704657121724	40.0	37.0	40.0	34.0	41.0
39	37.95752324598478	40.0	37.0	40.0	34.0	41.0
40	37.92416657861369	40.0	37.0	40.0	33.0	41.0
41	37.83637336221471	40.0	37.0	40.0	33.0	41.0
42	37.78160331255283	40.0	37.0	40.0	33.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.223280102300759
1101	2	5.554887197811851
1101	3	4.859105084045428
1101	4	4.809666882003
1101	5	7.871115909238398
1101	6	6.934854574626993
1101	7	6.0311867589823684
1101	8	6.181504596726732
1101	9	5.881220360419867
1101	10	5.315939912107616
1101	11	3.1440192194816525
1101	12	7.4875897582275925
1101	13	0.17348262261661773
1101	14	0.8751385180361986
1101	15	1.4910233455786432
1101	16	0.8722894964367214
1101	17	0.3943506543140032
1101	18	0.1323501039470827
1101	19	0.10103222903069309
1101	20	0.05985993368076947
1101	21	-0.04878631546288403
1101	22	0.9285207031239224
1101	23	0.43371436688732246
1101	24	-0.16052063892595925
1101	25	11.17070405816828
1101	26	6.492089514182272
1101	27	2.529172672811754
1101	28	1.0566970607426498
1101	29	0.2794553052732809
1101	30	0.08112817517519488
1101	31	-0.19944381986865523
1101	32	-0.29337477326269834
1101	33	0.0602595352814177
1101	34	0.066070042852445
1101	35	0.06779416782003977
1101	36	-0.046526508195483984
1101	37	0.02168908050259688
1101	38	-0.014914766498009158
1101	39	0.02782331822434969
1101	40	-0.08911264589078627
1101	41	0.09395540957021353
1101	42	0.0339253145510483
1102	1	3.796726847862889
1102	2	3.403360048490587
1102	3	2.1201562500739684
1102	4	1.3807337111015023
1102	5	4.509586149405475
1102	6	3.7542767813795166
1102	7	2.6273319034306795
1102	8	3.2089976353623015
1102	9	4.320356281060313
1102	10	3.3121677227585025
1102	11	3.6198371798018734
1102	12	5.572418334628566
1102	13	-0.048397815949343226
1102	14	1.1956353854222073
1102	15	1.6068691513565767
1102	16	0.9437000429038278
1102	17	0.35621107895723014
1102	18	0.05238143008701002
1102	19	0.00721043996004056
1102	20	-0.09170202246263415
1102	21	-0.10817110488160608
1102	22	0.43678732338149473
1102	23	0.13574621512957918
1102	24	-0.11454952858832712
1102	25	11.33709372054211
1102	26	6.239483353374748
1102	27	2.28322331673796
1102	28	0.6660383416337083
1102	29	0.04537507554825737
1102	30	-0.033238340661910115
1102	31	-0.4569142340698349
1102	32	-0.48804497862295193
1102	33	-0.2657020031801238
1102	34	-0.21469048621125353
1102	35	-0.11536368111835316
1102	36	-0.13849048313457502
1102	37	-0.21122512068083665
1102	38	-0.21551083332710874
1102	39	-0.2526283002928764
1102	40	-0.43823377363182203
1102	41	-0.1633279179619791
1102	42	-0.27930562871382847
1103	1	1.5180699055207931
1103	2	-2.9774539049977875
1103	3	-1.4828264112718408
1103	4	-0.6337667705277816
1103	5	1.3064073961372742
1103	6	0.3317502917689481
1103	7	-2.488585944374467
1103	8	-0.624702653615266
1103	9	2.391534208678312
1103	10	1.3769640623759507
1103	11	-1.6540550728470862
1103	12	1.8210594520085053
1103	13	-0.5371311309348954
1103	14	1.3276706131640523
1103	15	1.7297190206286288
1103	16	0.8799357660357927
1103	17	0.5474523064238639
1103	18	0.18059802565601757
1103	19	0.23397182705237185
1103	20	0.23823123747957453
1103	21	0.11090141472758575
1103	22	0.7942078545497893
1103	23	0.4327738744112679
1103	24	0.24433309147497084
1103	25	11.419589936312025
1103	26	6.619471656663588
1103	27	2.8193455129674945
1103	28	1.0402298924799993
1103	29	0.4472431091246989
1103	30	0.07722677561716296
1103	31	-0.005593193750584646
1103	32	0.018904243889778627
1103	33	-0.02665906936974949
1103	34	0.22546707536849198
1103	35	0.10800909994673447
1103	36	0.019535524846709507
1103	37	0.12278478712155305
1103	38	0.05464879237813136
1103	39	-0.048572293687676904
1103	40	-0.10597837250343645
1103	41	0.17278051743089407
1103	42	0.1284967656717484
1104	1	0.9803810412678935
1104	2	0.7967782500073781
1104	3	-0.9821335240738769
1104	4	-0.8510575955814623
1104	5	1.1335733335226692
1104	6	-0.11009617562615759
1104	7	-0.6768496950358589
1104	8	-0.3848111079588996
1104	9	1.9964751242972483
1104	10	0.9100126820606675
1104	11	0.359696288431298
1104	12	2.279494618763355
1104	13	0.18614371991938583
1104	14	1.8553385088306378
1104	15	1.6791228576839536
1104	16	0.7741731841841215
1104	17	0.19909381918558466
1104	18	-0.02782664280795899
1104	19	0.024794081189398298
1104	20	-0.004573233952477551
1104	21	0.10793949692780558
1104	22	0.8732067935225203
1104	23	0.2553163644938792
1104	24	-0.07087459271404128
1104	25	11.338214184284457
1104	26	6.36367310066851
1104	27	2.4696138492823394
1104	28	0.5895620612029262
1104	29	0.15748048248031665
1104	30	-0.11127493630414165
1104	31	-0.11749937542420952
1104	32	-0.2808471565822259
1104	33	-0.20278819554800265
1104	34	-0.21564311189302288
1104	35	-0.2291162160518212
1104	36	-0.4326272630514225
1104	37	-0.5784478522047607
1104	38	-0.3193829152598653
1104	39	-0.5066043260728037
1104	40	-0.5645591155584668
1104	41	-0.3997318775512042
1104	42	-0.24815951468580977
1105	1	0.4056270869992282
1105	2	1.339393074870145
1105	3	-2.814235044085031
1105	4	-0.9698666595864545
1105	5	-1.195296173146307
1105	6	-0.7626198787006899
1105	7	-1.3650699255096121
1105	8	-3.482708221613926
1105	9	1.6657871259199446
1105	10	0.4388134494613496
1105	11	0.8998883358050485
1105	12	2.803000490477288
1105	13	0.2810877213617857
1105	14	1.7949888118891124
1105	15	1.515692430134159
1105	16	0.7544021930004945
1105	17	0.1671216078799489
1105	18	0.032519532654660566
1105	19	-0.0967532848148025
1105	20	0.03836697556143065
1105	21	-0.13370649865767348
1105	22	0.6956157949860469
1105	23	0.22788826957171437
1105	24	-0.08917924008019185
1105	25	11.57571610349046
1105	26	6.224159853569152
1105	27	2.5222691148088927
1105	28	0.8411844065360512
1105	29	0.23550377776759035
1105	30	-2.155744512322144E-5
1105	31	-0.06149377486415375
1105	32	-0.4738805444280487
1105	33	-0.3670919498670955
1105	34	-0.38335284061237473
1105	35	-0.22796680533610214
1105	36	-0.2134356255778158
1105	37	-0.2941412525307072
1105	38	-0.12239279371249978
1105	39	-0.13278384543318822
1105	40	-0.38309660311004023
1105	41	-0.20351507277213443
1105	42	-0.40572034119099243
1106	1	-3.8527796525511313
1106	2	-4.302805976495666
1106	3	-1.4882406468925033
1106	4	-6.16398270040402
1106	5	-0.37381739605511655
1106	6	0.45045873253121727
1106	7	-1.203680752884484
1106	8	-3.4243439582212964
1106	9	-0.4963099826673165
1106	10	0.25298025940077196
1106	11	0.6760086444651279
1106	12	2.823419386299456
1106	13	0.15799788428818928
1106	14	1.7373425978295067
1106	15	1.5622139362173506
1106	16	0.728319809481242
1106	17	0.24290109594111442
1106	18	-0.04421061226994283
1106	19	-0.07385661312516589
1106	20	0.13348322875978624
1106	21	-0.0075219700924549215
1106	22	0.712130929645582
1106	23	0.6377528515355237
1106	24	0.13752208173738723
1106	25	11.641887889208359
1106	26	6.581151732532323
1106	27	2.7959672711413788
1106	28	1.0283525615538025
1106	29	0.216440073644371
1106	30	0.2103972372433347
1106	31	-0.21056486174811795
1106	32	-0.07452375481650364
1106	33	-0.12088193560724392
1106	34	-0.15866407960144358
1106	35	-0.12319228294003892
1106	36	-0.2415912501290336
1106	37	-0.3054176610704431
1106	38	-0.2394348349952793
1106	39	-0.09449462325254387
1106	40	-0.07461856429492286
1106	41	-0.07352791675316439
1106	42	-0.24748654028281436
1107	1	3.001895804780098
1107	2	2.340661383519194
1107	3	0.2900117442301635
1107	4	-4.092156161263894
1107	5	-22.780354678996893
1107	6	-17.547498366549476
1107	7	-8.170855049476554
1107	8	-5.151802403759415
1107	9	0.953406796977962
1107	10	1.6621175980580283
1107	11	2.227684751810287
1107	12	4.223874396049663
1107	13	-0.1675662349429352
1107	14	1.8506307397182589
1107	15	1.6196937393559878
1107	16	0.6840724327517407
1107	17	0.45977360035192305
1107	18	-0.22277872444377778
1107	19	0.08604681337683218
1107	20	-0.1236901392409564
1107	21	-0.4270131020452794
1107	22	0.21018088785897504
1107	23	0.4545882122932525
1107	24	0.21292369748629625
1107	25	11.75229739798355
1107	26	6.2796381286693865
1107	27	2.7506055848195317
1107	28	0.7360784413940848
1107	29	0.36380751723244487
1107	30	-0.2107653590980192
1107	31	-0.36359671243063474
1107	32	-0.15753981944646256
1107	33	-0.42662063000783945
1107	34	-0.1620534379448344
1107	35	-0.7089012624181734
1107	36	-0.9013089583656395
1107	37	-0.5982489360263301
1107	38	-0.39413815006631125
1107	39	-0.05725811104156975
1107	40	-0.54425362790343
1107	41	-0.514005212695217
1107	42	-0.35752461252722156
1113	1	-10.564219897699243
1113	2	-9.051310281179745
1113	3	1.116458025221899
1113	4	-0.004198664215486758
1113	5	2.933931035288822
1113	6	-17.547498366549476
1113	7	-7.949485509925196
1113	8	-0.01839036125646132
1113	9	-20.707750227815428
1113	10	-21.296560087892384
1113	11	-9.715644646064566
1113	12	-21.598439653537113
1113	13	0.09984816883510206
1113	14	1.871882215515189
1113	15	1.9130821691080548
1113	16	0.23247857206697375
1113	17	-0.8029182532490253
1113	18	0.3256274148714553
1113	19	-0.13237113231384257
1113	20	0.8881162362017818
1113	21	0.8622430963018246
1113	22	2.58503330816594
1113	23	2.3081891568032944
1113	24	0.8203617140152204
1113	25	12.782993974134666
1113	26	7.718244976367142
1113	27	3.4696138492823394
1113	28	2.0601634472972705
1113	29	0.6961569859455494
1113	30	1.1233550659314986
1113	31	0.7951990372742017
1113	32	1.3023185040482232
1113	33	0.7906166781385622
1113	34	-0.5746862596685673
1113	35	-0.782100790163156
1113	36	-0.5760433149181736
1113	37	0.6520462233597399
1113	38	0.4393919561910664
1113	39	-1.1829960095067378
1113	40	-1.3376420576554935
1113	41	-0.03230509463146092
1113	42	0.13066901203004022
1114	1	-8.579683815225017
1114	2	-0.9298080720486688
1114	3	-0.23405743869562556
1114	4	-7.585936513994575
1114	5	0.03407831069382894
1114	6	0.3081717365433079
1114	7	-0.1026519311328542
1114	8	1.0699748817479566
1114	9	-4.666513114413366
1114	10	0.8168419739632853
1114	11	1.4434127913433876
1114	12	-21.598439653537113
1114	13	-0.15862016695281866
1114	14	-27.413832070199096
1114	15	-13.89619618140741
1114	16	-5.5981547121745585
1114	17	-3.7528446155465183
1114	18	-1.5823254569989444
1114	19	-0.8098379953477135
1114	20	-1.0272004059189825
1114	21	-0.5140455634919903
1114	22	1.218317549697602
1114	23	1.1189402613688273
1114	24	0.3719081057678011
1114	25	11.802139776785626
1114	26	6.99438636075595
1114	27	2.8459025090761543
1114	28	1.9143607963399845
1114	29	0.9060244380810474
1114	30	0.7235023413365056
1114	31	0.04630360281176138
1114	32	0.04532292231037616
1114	33	0.14775953528141628
1114	34	-0.16967889589831486
1114	35	-0.11126132035461467
1114	36	-0.06647041359269679
1114	37	-0.4650377236211156
1114	38	-0.6283547301123207
1114	39	-0.011420162673161371
1114	40	0.0798837155403831
1114	41	0.0877243604495348
1114	42	0.10784132425389714
1115	1	-8.064219897699243
1115	2	-0.5036912335606978
1115	3	1.687886596650472
1115	4	1.186277526260703
1115	5	-1.4946403932826087
1115	6	3.0715492524981443
1115	7	3.0505144900748036
1115	8	2.838752495886393
1115	9	-20.707750227815428
1115	10	-21.296560087892384
1115	11	-8.287073217493138
1115	12	-21.598439653537113
1115	13	0.5046100735970072
1115	14	2.0147393583723314
1115	15	-27.586917830891945
1115	16	-14.76752142793303
1115	17	-3.6600611103918794
1115	18	-1.1267535375095008
1115	19	-1.703799703742412
1115	20	0.10240195048749712
1115	21	0.1241478582065838
1115	22	1.58503330816594
1115	23	0.7605701091842434
1115	24	1.3203617140152204
1115	25	13.449660640801337
1115	26	6.8372925954147625
1115	27	2.493423373091865
1115	28	2.0601634472972705
1115	29	1.62472841451698
1115	30	1.099545542121973
1115	31	1.5809133229884935
1115	32	0.8499375516672742
1115	33	1.1001404876623653
1115	34	1.139599454617148
1115	35	1.0036134955511287
1115	36	-0.09985283872769912
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2206	38	-0.02930535751018226
2206	39	0.07265196171951516
2206	40	0.17258610914230132
2206	41	0.12420833686229571
2206	42	0.04125685062732032
2207	1	-0.983411816891163
2207	2	-0.19055992042938286
2207	3	-3.7135083047444297
2207	4	-1.2136743736911981
2207	5	-0.9537553523975681
2207	6	-0.254569073620182
2207	7	-2.5233190837587713
2207	8	-0.507327350193453
2207	9	1.7653137452485446
2207	10	1.2169079255756294
2207	11	1.5510701206501984
2207	12	1.1911226360251774
2207	13	0.26218583117276495
2207	14	1.9565383001712746
2207	15	1.7598835159094008
2207	16	0.9133347529231557
2207	17	0.24566279533202362
2207	18	-0.09621000696596838
2207	19	0.08864858870587966
2207	20	0.13174297982852323
2207	21	0.32857306263179
2207	22	0.8736335967662257
2207	23	0.3606182092323422
2207	24	0.27490716856067365
2207	25	6.3888140799547735
2207	26	4.00323776135993
2207	27	1.6042939839624708
2207	28	0.6777680649018905
2207	29	0.45349224328081306
2207	30	0.29459123716766555
2207	31	0.30000904208421275
2207	32	0.1635498652795846
2207	33	0.07200195952383837
2207	34	0.24060955562725184
2207	35	0.26431575625339576
2207	36	0.03242229354743387
2207	37	-0.02448095316744059
2207	38	-0.11039159359248885
2207	39	0.09333876682803322
2207	40	-0.11710333711677379
2207	41	-0.06741812974449601
2207	42	0.18670556806659988
2208	1	0.911970578491232
2208	2	-23.83702456689403
2208	3	-7.4311610223971485
2208	4	-2.5756272356440597
2208	5	3.267264368622154
2208	6	4.660834966783856
2208	7	3.663609728170041
2208	8	-24.589818932685034
2208	9	3.3934402483750468
2208	10	3.012963721631426
2208	11	-21.144216074635995
2208	12	-6.640106320203781
2208	13	0.6355624545493903
2208	14	2.1397393583723314
2208	15	2.3237964548223395
2208	16	1.3158119054003024
2208	17	0.7744626991319308
2208	18	0.3018178910619298
2208	19	0.42120029625758804
2208	20	0.5607352838208257
2208	21	0.5884335724922991
2208	22	1.5433666414992686
2208	23	1.4272367758509077
2208	24	0.6536950473485561
2208	25	-22.07414888300819
2208	26	-12.74008835696619
2208	27	-4.274433769765281
2208	28	-1.1541222669884377
2208	29	-0.5955096807211149
2208	30	0.010259827836257784
2208	31	0.6225799896551578
2208	32	0.8618423135720334
2208	33	0.7727595352814163
2208	34	1.091980406998097
2208	35	0.7119468288844644
2208	36	0.9358614469865856
2208	37	0.9020462233597399
2208	38	0.7786776704767817
2208	39	1.0431944666837367
2208	40	0.9599769899635575
2208	41	0.937933000606634
2208	42	0.6128118691729014
2209	1	-2.577040410519757
2209	2	-3.3669390968085615
2209	3	-3.1399522311883565
2209	4	-5.122635782652608
2209	5	-2.925653824296038
2209	6	1.1106212915701832
2209	7	-5.964137524577211
2209	8	-6.07699841986452
2209	9	-3.006895526960726
2209	10	-0.19399598532828222
2209	11	-1.7168656472855695
2209	12	0.06822701312955459
2209	13	-0.31590274691581044
2209	14	1.1759115195444956
2209	15	1.3019710579969441
2209	16	0.23125757084596898
2209	17	0.09207564174487004
2209	18	-0.449097859853822
2209	19	-0.45227345221616133
2209	20	-0.7022378541523082
2209	21	-0.5693808353221073
2209	22	0.30176102489365775
2209	23	-0.7363773877632553
2209	24	-1.0984417047882005
2209	25	-3.4758582847175887
2209	26	-1.79396503584287
2209	27	-1.6030357233672348
2209	28	-1.532022144888316
2209	29	-1.0285072387186744
2209	30	-1.202041759465331
2209	31	-1.5363027942276233
2209	32	-1.5896228878931637
2209	33	-1.954804567282686
2209	34	-3.0398877248700344
2209	35	-1.8278883359506963
2209	36	-2.303760042634906
2209	37	-1.8711527998392867
2209	38	-2.476358959559853
2209	39	-2.6207249472356793
2209	40	-3.094052314065749
2209	41	-2.6409742033005728
2209	42	-1.7234213420603126
2215	1	-11.1287360267315
2215	2	-2.1596052120553217
2215	3	-2.496445200584553
2215	4	0.19856631274303638
2215	5	-1.2964837112549574
2215	6	3.194437117321492
2215	7	2.3270121859273374
2215	8	2.4101810673149657
2215	9	-20.707750227815428
2215	10	-8.619140733053674
2215	11	-1.5313128488295433
2215	12	-21.598439653537113
2215	13	-0.31259422747826093
2215	14	-26.381574005682968
2215	15	-13.006272669601621
2215	16	-5.067060598439941
2215	17	-3.9181256265209115
2215	18	-1.280363368538687
2215	19	-0.5148596115765116
2215	20	0.15770148965800246
2215	21	0.20095277372117692
2215	22	1.8983973634654774
2215	23	0.11694491717195632
2215	24	1.223587520466836
2215	25	9.861334987959555
2215	26	6.932530690652857
2215	27	4.034129978314596
2215	28	2.1707625256382883
2215	29	2.6293367094478555
2215	30	0.7477790290651356
2215	31	1.2122497285184437
2215	32	0.3276641261680382
2215	33	0.5025982449588327
2215	34	0.8170188094558597
2215	35	0.8884061222792425
2215	36	0.4792562242523317
2215	37	0.17047940308324172
2215	38	0.07533665665189204
2215	39	-0.6000467007509798
2215	40	0.8397772971832183
2215	41	0.4216119560597775
2215	42	-0.2679484994906929
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	14.0
25	156.0
26	1151.0
27	4226.0
28	10734.0
29	20512.0
30	32524.0
31	44472.0
32	51780.0
33	52209.0
34	41484.0
35	27147.0
36	10852.0
37	5438.0
38	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.120811152004168	1.742333665079185	95.54915070835533	1.5877044745613271
2	92.02741427228986	2.3944321400465536	1.1176894443761891	4.460464143287388
3	2.6349852071005917	88.17294484361791	4.904440511411665	4.287629437869823
4	0.9073858833474218	3.264674027895182	0.8717244294167372	94.95621565934066
5	94.18001466801068	3.814017661892307	0.742777344286341	1.2631903258106754
6	2.8738177655428054	89.12446509853089	2.7423069584889204	5.259410177437379
7	1.2187712566783118	1.36769183017111	93.70868362137853	3.7048532917720554
8	1.7512227678882724	3.430059004984045	2.8951291833936	91.92358904373408
9	27.992161691591694	28.905086294214616	24.563563782248615	18.53918823194508
10	34.081914038864035	27.131421542856575	20.111162912190558	18.675501506088832
11	32.28760166989375	24.992610354802604	19.803252772362395	22.916535202941247
12	28.777508791854707	24.740063294712268	26.935121259518287	19.54730665391474
13	31.623155280159597	26.989060497562395	19.25279763241686	22.134986589861143
14	28.02540166802076	27.594634477955925	22.113710600056375	22.266253253966937
15	22.884002180844913	25.57800650949163	30.15645910089712	21.381532208766334
16	23.04885619188504	28.090329142011832	28.551616652578193	20.309198013524938
17	20.933550417600998	28.81364328387291	26.481418489370906	23.771387809155186
18	23.05050718512257	28.99144125105664	22.007409657650044	25.950641906170752
19	22.616956360946748	30.362756234150467	24.19992867709214	22.82035872781065
20	21.387296597633135	29.97180103550296	25.05844516060862	23.582457206255285
21	21.26908548182587	27.21563292476754	26.96963493237532	24.545646661031277
22	22.55238589556758	28.450082845906614	27.386727353788622	21.61080390473719
23	21.149223372781066	29.376783072696533	27.794471153846157	21.679522400676245
24	20.66812394336433	27.778291420118347	29.57622305579036	21.977361580726964
25	19.382007082413995	29.57879061057182	27.513833618057664	23.52536868895651
26	22.67768223904056	30.364478081124275	27.400245671038554	19.55759400879661
27	22.34542422691476	30.894351896184517	27.259819379550592	19.50040449735013
28	21.797907152598228	30.93141285360965	26.666138336618882	20.604541657173236
29	21.150944206150303	31.76224298077907	27.0783596997824	20.008453113288226
30	23.475803043110734	30.80059964074387	25.94733991969569	19.776257396449704
31	21.687447168216398	32.64211749788673	26.267302409129336	19.40313292476754
32	19.980980557903635	32.99179786559594	27.744941356720204	19.28228021978022
33	20.36599218089603	32.168942836010146	26.91878434065934	20.54628064243449
34	21.943020921386307	31.194526627218934	27.103035185967876	19.759417265426883
35	22.41883717244294	33.17802990278952	26.67377694420964	17.729355980557905
36	20.22301616652578	32.29012573964497	27.557058326289095	19.929799767540153
37	22.311852810650887	31.21136675824176	26.04805050718512	20.42872992392223
38	22.48322590870668	31.34674820371936	26.607737214708372	19.562288672865595
39	21.12875105663567	32.13856456043956	27.32657967032967	19.406104712595095
40	20.31976437024514	32.11346946322908	26.280510355029584	21.286255811496197
41	21.115212912087912	31.809686707523245	27.47186707523246	19.60323330515638
42	22.919088123415047	30.74116388419273	27.63234361792054	18.707404374471682
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.0
7	6.0
8	23.0
9	40.0
10	40.0
11	74.5
12	109.0
13	228.5
14	348.0
15	589.5
16	831.0
17	831.0
18	1202.0
19	1573.0
20	2007.0
21	2441.0
22	2441.0
23	3000.5
24	3560.0
25	4329.0
26	5098.0
27	6054.5
28	7011.0
29	7011.0
30	8895.0
31	10779.0
32	13125.5
33	15472.0
34	15472.0
35	17884.0
36	20296.0
37	22237.5
38	24179.0
39	23279.0
40	22379.0
41	22379.0
42	23383.0
43	24387.0
44	24670.5
45	24954.0
46	25131.0
47	25308.0
48	25308.0
49	25830.0
50	26352.0
51	25779.0
52	25206.0
53	25206.0
54	23245.5
55	21285.0
56	18669.0
57	16053.0
58	13056.5
59	10060.0
60	10060.0
61	8199.5
62	6339.0
63	5393.5
64	4448.0
65	3245.5
66	2043.0
67	2043.0
68	1573.5
69	1104.0
70	844.0
71	584.0
72	584.0
73	451.0
74	318.0
75	226.0
76	134.0
77	114.5
78	95.0
79	95.0
80	71.5
81	48.0
82	27.5
83	7.0
84	7.0
85	4.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	1.1299397717666948
2	0.5583659129332206
3	0.0
4	0.0
5	1.3997120667793743
6	3.0827345731191884
7	6.603972950126796E-4
8	0.5570451183431953
9	0.24500739644970412
10	0.13505124683009298
11	0.578177831783601
12	0.5669510777683855
13	0.030378275570583264
14	0.4269468512256974
15	0.07033231191885038
16	0.0
17	0.015519336432797971
18	0.0
19	0.0
20	0.0
21	0.0
22	0.7561549027895181
23	0.0
24	0.0
25	33.7030457523246
26	0.0013207945900253592
27	9.905959425190194E-4
28	0.002311390532544379
29	3.301986475063398E-4
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
42	302848.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.011355587694325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.83602557030872	44.94548686909433
2	10.68228914219035	12.180235695519603
3	4.372882277990788	7.479118394809789
4	2.0933906960299096	4.773881654213284
5	1.156263298765207	3.2960069039451834
6	0.6335371570414299	2.167128728286354
7	0.3791169306862216	1.5129779101267207
8	0.29275612845102145	1.3352338991678319
9	0.2220933646042548	1.1395659404808554
>10	1.1942737456273316	12.665630122752319
>50	0.0981226345034067	3.7982273739528227
>100	0.036870323267946765	3.8572197476980508
>500	0.0023787305334159203	0.8492867599528537
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGACGATCGAGGAAAATCACGAAAAATGAGAAATACACACTT	737	0.24335640321217245	No Hit
TGACGACTGGGTAGTGGACATTTCTAAATTTTCCACCTTTTT	733	0.24203560862214707	No Hit
TGACGATCGAGGAAAATCACGAAANATGAGAAATACACACTT	518	0.17104289940828404	No Hit
TGACGATCAAAAGAGTCGTATTACCGGCCGACGATCAAAAAG	517	0.17071270076077769	No Hit
TGACGATCTGGGTGTGACCCCACTCTCCCACTCTGAGCAAAG	499	0.16476912510566358	No Hit
TGACGACTGGGTAGTGGACATTTCNAAATTTTCCACCTTTTT	467	0.15420276838546068	No Hit
TGACAATCAAGCGTATCAAATGATGGCCTAGTCGGCCATCAC	381	0.12580568469991546	No Hit
TGACGATCAAAAGAGTCGTATTACNGGCCGACGATCAAAAAG	379	0.1251452874049028	No Hit
TGACGATCTGGGTGTGACCCCACTNTCCCACTCTGAGCAAAG	326	0.10764475908706678	No Hit
TGACGATCGGGTGCCGACGATCGTCAACCCGATCGTCGGACT	307	0.10137098478444632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	3.301986475063398E-4	0.0	0.0
11	0.0	0.0	9.905959425190194E-4	0.0	0.0
12	0.0	0.0	0.01882132290786137	0.0	0.0
13	0.0	0.0	0.07198330515638209	0.0	0.0
14	0.0	0.0	0.1069843617920541	0.0	0.0
15	0.0	0.0	0.2139687235841082	0.0	0.0
16	0.0	0.0	0.2988297759932375	0.0	0.0
17	0.0	0.0	0.35034076500422656	0.0	0.0
18	0.0	0.0	0.42859784446322907	0.0	0.0
19	0.0	0.0	0.510156910397295	0.0	0.0
20	0.0	0.0	0.5758664412510567	0.0	0.0
21	0.0	0.0	0.7898351648351648	0.0	0.0
22	0.0	0.0	0.988284551986475	0.0	0.0
23	0.0	0.0	1.1226754015215554	0.0	0.0
24	0.0	0.0	1.281831149619611	0.0	0.0
25	0.0	0.0	1.4390057058326289	0.0	0.0
26	0.0	0.0	1.736514687235841	0.0	0.0
27	0.0	0.0	2.120865912933221	0.0	0.0
28	0.0	0.0	2.373798076923077	0.0	0.0
29	6.603972950126796E-4	0.0	2.838057375316991	0.0	0.0
30	6.603972950126796E-4	0.0	3.8365780853761624	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCTC	15	0.007923199	50.776463	21
GCCTCGC	20	4.096331E-4	50.19457	23
CTCTCCC	140	0.0	43.02392	23
ACAGGCA	85	0.0	41.81591	21
TACCATA	25	0.0011617971	40.62117	19
GACAGGC	95	0.0	37.414238	20
CACTCTC	165	0.0	36.928333	21
CTCGATA	35	1.3831856E-4	36.2689	20
CAGGCAG	100	0.0	35.54352	22
AGCCGAT	30	1.2241175E-5	34.955067	1
CGACGCT	30	1.2241175E-5	34.955067	1
TGGCGTT	70	0.0	34.955067	1
TGGCGGT	75	0.0	34.955067	1
TGACGTT	420	0.0	34.955067	1
TGACGTC	30	1.2241175E-5	34.955067	1
TAACGCT	35	8.757452E-7	34.955067	1
AGACGAG	80	0.0	34.955067	1
TTACGCT	20	0.0024317116	34.955067	1
TCACGCT	25	1.7202523E-4	34.955063	1
TGCCGTT	25	1.7202523E-4	34.955063	1
>>END_MODULE