FastQCFastQC Report
Thu 26 May 2016
SRR2099044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875624
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTAGCGCTCTAGGACGATCAGTGTGATCTAGCTTTTTTTT11910.13601728595835655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCT150.007155993552.11416623
TCGAACG150.007155993552.11416623
TAGCGCC14550.035.6358955
TAGCCGT10550.035.6358955
CTAGCGC51000.035.5310824
CTAGCGT50000.035.4577144
TAGCGTT17200.035.3251155
TAGCGTC9700.035.2685135
TAGCGCG12250.035.1995355
CTAGCCC48200.035.192294
TAGCGGG11950.035.1885835
TAGCACG11800.035.1828965
TAGCGCT14750.035.1526955
TAGCGTA10900.035.1454935
CTAGCGA34500.035.1194344
TCTAGCG175150.035.1170733
CTAGCAC46500.035.099444
TCTAGCC175200.035.096883
TAGCGGT12550.035.0679935
TAGCCCG9200.035.0548745
Produced by FastQC (version 0.11.5)