FastQCFastQC Report
Thu 26 May 2016
SRR2099043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302457
Sequences flagged as poor quality0
Sequence length43
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTACGCATGGCAAGGATAGACAATTTAGGAATCGGAGA6250.2066409440019573No Hit
TGCACGTACGCATGGGCAAGGATAGACAATTTAGGAATCGGAG5620.18581153684656002No Hit
TGCACGTAGTCCAAATTTAACGTCCGAAACTCGAATAGCCATT5170.17093338887841908No Hit
TGCACGTATAAAAGTTCTACAGTGAGTCGTATTACCGGCCGAC5040.16663525724317838No Hit
TGCACGTACGTTGACCAGCTGTCAGCACTGTACAGGGAGCACC4640.15341023682705313No Hit
TGCACGTATAAAACCGGCCGACGATCTAAAATACGTGCATTTT4280.14150771845254037No Hit
TGCACGTACGCATGGCAAGGATAGANAATTTAGGAATCGGAGA4080.1348952082444777No Hit
TGCACGTAGCGCCGTGGTTTGGCGCCTGCCTTTGGCCCAGGGC4070.1345645827340746No Hit
TGCACGTACGCATGGGCAAGGATAGNCAATTTAGGAATCGGAG3990.13191957865084955No Hit
TGCACGTCCCCATATGATAGTTAGATGATAAGAATGGTACTCA3970.13125832763004328No Hit
TGCACGTAGTCCAAATTTAACGTCCNAAACTCGAATAGCCATT3690.12200081333875558No Hit
TGCACGTATAAAAGTTCTACAGTGANTCGTATTACCGGCCGAC3390.11208204802666165No Hit
TGCACGTAACTCTAGGTGACGTAGGGTGTTTTTTGCCGTTTTT3270.10811454190182407No Hit
TGCACGTACGTTGACCAGCTGTCAGNACTGTACAGGGAGCACC3220.10646141434980841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT203.2378398E-452.64378724
AGTGCCC351.9437866E-645.6827720
TCCGAAT304.1909785E-544.41380322
CCGAATT304.1909785E-544.41380323
ACGCCTA259.153752E-442.63725323
TCCCTTG259.726426E-442.11503226
TCCGAAA1100.041.1837123
AAGCCGC404.869551E-639.9724221
TCCGTGA757.2759576E-1238.60544225
ATCCGAA351.0400098E-438.06897421
CCGAAAC1200.037.2893524
GTTGTCT300.002242540535.5310422
TCGCTTT451.0925845E-535.5310423
AAGCTCC300.002242540535.5310422
CGTATCC3850.035.4486275
CGTATAC2200.035.4486275
GGTAAGC301.112033E-535.4486275
CGCGAGG251.5841836E-435.4486275
CGTAGAC3700.035.4486275
CGAATAA251.5841836E-435.4486275
Produced by FastQC (version 0.11.5)