##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302457
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.693863921152428	30.0	26.0	31.0	17.0	35.0
2	27.975579999801624	30.0	26.0	31.0	17.0	35.0
3	28.454676201906388	30.0	27.0	31.0	19.0	35.0
4	25.019490373838266	28.0	19.0	31.0	10.0	34.0
5	27.91389189207061	30.0	27.0	31.0	17.0	37.0
6	27.7706880647497	30.0	25.0	31.0	17.0	37.0
7	26.783648584757504	29.0	23.0	31.0	13.0	37.0
8	24.100453948825784	27.0	17.0	30.0	8.0	34.0
9	25.423918110673583	26.0	26.0	27.0	18.0	31.0
10	25.587349606720956	27.0	26.0	27.0	18.0	31.0
11	25.28413956364045	27.0	25.0	27.0	16.0	31.0
12	27.500702579209605	30.0	25.0	31.0	17.0	35.0
13	27.236559246438336	30.0	25.0	31.0	17.0	35.0
14	30.854223906208155	31.0	31.0	33.0	28.0	34.0
15	30.809106749058543	31.0	31.0	33.0	28.0	34.0
16	31.062521284017233	31.0	31.0	34.0	28.0	34.0
17	34.90462776526911	35.0	35.0	37.0	32.0	37.0
18	34.996386263171296	35.0	35.0	37.0	32.0	37.0
19	34.844526659988034	35.0	35.0	37.0	32.0	37.0
20	34.79118354013959	35.0	35.0	37.0	32.0	37.0
21	34.82529086779278	35.0	35.0	37.0	32.0	37.0
22	36.405598151142144	37.0	35.0	39.0	32.0	39.0
23	36.20988438025901	37.0	35.0	39.0	32.0	39.0
24	36.475968484776345	37.0	35.0	39.0	32.0	39.0
25	36.61381948508383	37.0	35.0	39.0	32.0	39.0
26	22.82669602621199	32.0	2.0	37.0	2.0	39.0
27	29.6881011185061	34.0	17.0	38.0	17.0	40.0
28	34.59896117464631	36.0	32.0	39.0	27.0	40.0
29	36.50206145005736	37.0	35.0	39.0	32.0	40.0
30	37.249245347272506	38.0	36.0	40.0	33.0	40.0
31	37.60184092284192	39.0	37.0	40.0	33.0	41.0
32	37.89749947926482	39.0	37.0	40.0	34.0	41.0
33	38.01754629583709	39.0	37.0	40.0	34.0	41.0
34	38.053696889144575	39.0	37.0	40.0	34.0	41.0
35	38.07899304694551	39.0	37.0	40.0	34.0	41.0
36	38.08941105677832	39.0	37.0	40.0	34.0	41.0
37	38.12411681660534	40.0	37.0	40.0	34.0	41.0
38	38.06173108904737	39.0	37.0	40.0	34.0	41.0
39	37.922970868586276	39.0	37.0	40.0	33.0	41.0
40	37.80317202114681	39.0	37.0	40.0	33.0	41.0
41	37.71609187421683	39.0	37.0	40.0	33.0	41.0
42	37.59101293737622	39.0	37.0	40.0	33.0	41.0
43	37.531361482789286	39.0	37.0	40.0	33.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	5.764338849190068
1101	2	5.117429013767886
1101	3	5.28257692971658
1101	4	7.408974383942326
1101	5	8.144946554456862
1101	6	7.260529119687437
1101	7	6.420578417497666
1101	8	5.543581806986698
1101	9	5.819506356607615
1101	10	5.473748944328641
1101	11	3.472870927511785
1101	12	7.856118094072524
1101	13	6.535492952621947
1101	14	0.15900671996144666
1101	15	0.934636396349056
1101	16	1.5690693818888413
1101	17	0.79541442014186
1101	18	0.30624691770356804
1101	19	0.21431559311140091
1101	20	0.2015758102768146
1101	21	0.1076197126545324
1101	22	0.24657005146600142
1101	23	1.010568863757328
1101	24	0.7047231905432696
1101	25	0.35150447858607237
1101	26	12.407690038407068
1101	27	7.290429374090927
1101	28	2.8709680030611366
1101	29	1.3541409740947472
1101	30	0.8089352530457816
1101	31	0.4490003897229897
1101	32	0.2029645212063329
1101	33	-0.02602817733551177
1101	34	0.07198984941867081
1101	35	0.24270357702853573
1101	36	0.24829485379979843
1101	37	0.07493831754354119
1101	38	0.09770262666081919
1101	39	0.11708788453623953
1101	40	0.016200759094466832
1101	41	-0.21462851758636248
1101	42	-0.05910786023031278
1101	43	-0.08295323212237804
1102	1	3.1734223566897093
1102	2	2.1261225006473587
1102	3	1.8101276617746542
1102	4	4.07282361327389
1102	5	5.051059022526374
1102	6	3.3221965837420306
1102	7	3.386070825525266
1102	8	1.8977361980760996
1102	9	4.149964057276581
1102	10	3.3727688566439475
1102	11	3.754837929640953
1102	12	5.49629245623213
1102	13	4.042396093770801
1102	14	-0.23579598947878821
1102	15	1.4196469022847005
1102	16	1.7122461194251066
1102	17	0.9102321260707882
1102	18	0.34708437087451216
1102	19	0.15603353042272516
1102	20	0.0074154877403955766
1102	21	0.1016255937402093
1102	22	0.30529865139269674
1102	23	1.035889427692446
1102	24	0.7428735862994884
1102	25	0.3689613986542284
1102	26	12.307744036957779
1102	27	7.2293516973156215
1102	28	2.904618892638304
1102	29	1.2956977551096358
1102	30	0.6062901634165812
1102	31	0.47691288409231447
1102	32	0.29096145476013646
1102	33	0.08960482277360882
1102	34	0.15262843124326508
1102	35	0.08513636562000926
1102	36	0.054321427801596656
1102	37	0.055442882239681524
1102	38	-0.11516749423438455
1102	39	-0.2904159555161314
1102	40	-0.33329520780134914
1102	41	-0.4408494304136781
1102	42	-0.15013710618112697
1102	43	-0.21917786609333945
1103	1	0.3393919384387587
1103	2	-0.17349727121575853
1103	3	-0.14766701503142698
1103	4	0.6422152482548782
1103	5	1.877104649902801
1103	6	-2.211073378235156
1103	7	-1.3443474254253047
1103	8	-2.366332242988541
1103	9	2.0632720420674566
1103	10	1.419346803412008
1103	11	-1.3457324125643702
1103	12	1.665303863076236
1103	13	1.4903048390179485
1103	14	-0.6709670921403834
1103	15	1.2618522254786164
1103	16	1.6059723551665535
1103	17	1.0501560804434078
1103	18	0.3185672606083543
1103	19	0.17029208555580055
1103	20	0.3726246132156774
1103	21	0.16474346446264576
1103	22	0.2087207046246391
1103	23	1.1109844847266714
1103	24	0.7061815710903616
1103	25	0.5311286990344541
1103	26	12.595196508440672
1103	27	7.416804168798507
1103	28	3.108611288075565
1103	29	1.4464582113834012
1103	30	0.7610430151276049
1103	31	0.34250223770447974
1103	32	0.22099947757382665
1103	33	0.026677066119610515
1103	34	0.2100428799124643
1103	35	0.08152419831963442
1103	36	0.12029100955064109
1103	37	0.20468242596591324
1103	38	0.10384391260971881
1103	39	0.3025498239515443
1103	40	0.00776943098192362
1103	41	0.02131786996655194
1103	42	0.07172601168959858
1103	43	0.01846471945798811
1104	1	0.8001750441046305
1104	2	-1.0739954404876926
1104	3	-0.5743440088820613
1104	4	1.1002199893711548
1104	5	0.8669388281875463
1104	6	-0.9001077865344591
1104	7	-0.15214151545957222
1104	8	-0.30679837451931036
1104	9	1.9873789753632352
1104	10	0.6540643314597006
1104	11	0.23880380299212334
1104	12	2.4842964932222245
1104	13	1.1409115610758747
1104	14	0.2896634528532793
1104	15	2.0575617499587366
1104	16	1.797122075863168
1104	17	0.8797135371396507
1104	18	0.4510838075735748
1104	19	0.10788010132822734
1104	20	0.01954347440894111
1104	21	-0.02896458135249702
1104	22	0.06300472565453674
1104	23	0.9554987550172385
1104	24	0.5475712814597955
1104	25	0.20779689130784362
1104	26	12.182871953213706
1104	27	7.077098493541506
1104	28	2.710172687878405
1104	29	1.1130016559686737
1104	30	0.4803695184370014
1104	31	0.24463750034354348
1104	32	0.0358788372884149
1104	33	0.02352316760766371
1104	34	0.041064332290062566
1104	35	0.051141153678031515
1104	36	-0.012906192721338527
1104	37	-0.22260481057354298
1104	38	-0.31325109748275537
1104	39	-0.3113572548636441
1104	40	-0.3388601517059584
1104	41	-0.2890756192133779
1104	42	-0.36880759860002854
1104	43	-0.1379569112982395
1105	1	0.7759085972925206
1105	2	-2.259199651548066
1105	3	-1.174614599106988
1105	4	-1.1483129042530642
1105	5	-2.3361243377180827
1105	6	-2.1736660479922634
1105	7	-5.186056194979276
1105	8	-2.9958902060768935
1105	9	1.4088317066333182
1105	10	0.40732621206176134
1105	11	0.8086879565520739
1105	12	2.6104338177613755
1105	13	0.09664891943880605
1105	14	0.26951505102679363
1105	15	1.7015157496205013
1105	16	1.6336031345474247
1105	17	0.6738725934562382
1105	18	0.12287477662481194
1105	19	-0.12435607320246334
1105	20	-0.12438304343310591
1105	21	0.014200901481935091
1105	22	0.0715642555040219
1105	23	0.7071709180478507
1105	24	0.26911280014743255
1105	25	0.11929908985342053
1105	26	12.038216110156036
1105	27	6.715175611755605
1105	28	2.7709730430280786
1105	29	1.0307464555627845
1105	30	0.3195564793705401
1105	31	0.14784018933390541
1105	32	0.08816617028351459
1105	33	-0.27959496193740563
1105	34	-0.22368283496852115
1105	35	-0.022324092389951034
1105	36	-0.3804269097854345
1105	37	-0.11941691948610611
1105	38	-0.4854437238991238
1105	39	-0.19086215931147166
1105	40	-0.5787975777164434
1105	41	-0.6145216702872816
1105	42	-0.37529119406764266
1105	43	-0.6398594988172661
1106	1	-4.356098485850335
1106	2	-2.2160355674961743
1106	3	-6.727379284280204
1106	4	-1.2644943125794335
1106	5	0.4983718290825756
1106	6	-1.972303015902682
1106	7	-4.628667499633234
1106	8	-2.949896017616954
1106	9	-0.31789244488708945
1106	10	0.20744181231608394
1106	11	0.8338772519685236
1106	12	3.0234041662961424
1106	13	-3.671823738473769
1106	14	0.3339432764052539
1106	15	1.9604371992476892
1106	16	1.743719733167577
1106	17	0.9234819171422046
1106	18	0.3400792472019205
1106	19	0.10488868931783202
1106	20	0.3124345866565932
1106	21	0.1360141440165208
1106	22	0.08545940607335467
1106	23	1.0277073032278992
1106	24	0.5766294439567758
1106	25	0.3617138740750363
1106	26	12.255472075391886
1106	27	7.018293140432348
1106	28	2.682584037663176
1106	29	1.1816363147932023
1106	30	0.334765269193511
1106	31	0.33343955037977224
1106	32	-0.22843980624963223
1106	33	-0.09350062162076256
1106	34	0.0866218767927549
1106	35	-0.18109683805362664
1106	36	-0.022151245215333404
1106	37	-0.28027362246176324
1106	38	-0.24147674353540083
1106	39	0.09106269001410539
1106	40	0.1187291022967969
1106	41	-0.07741346319468079
1106	42	-0.09720532409691174
1106	43	-0.1581269662784237
1107	1	1.5583850497867395
1107	2	1.1733216988273547
1107	3	-4.015947397486844
1107	4	-20.953792356307858
1107	5	-23.390005616256897
1107	6	-9.765351342503259
1107	7	-6.672497034856235
1107	8	-2.907294267902234
1107	9	1.3560317983396288
1107	10	1.869006348092789
1107	11	2.2896264177966046
1107	12	4.205756795923811
1107	13	2.5943505300308836
1107	14	0.3319403008309614
1107	15	1.924316231078631
1107	16	1.8968269934725477
1107	17	1.1460722075255134
1107	18	0.2287841020102661
1107	19	0.2498973801132891
1107	20	-0.07374969672593323
1107	21	0.1893867022881821
1107	22	-0.142600548378212
1107	23	1.2707558705707314
1107	24	0.8511574687628567
1107	25	0.6545238658416537
1107	26	12.738569561561249
1107	27	7.4776486252631855
1107	28	3.0608719750316666
1107	29	1.475178163019656
1107	30	1.1132631338096957
1107	31	0.49098609789507464
1107	32	0.2560154662465237
1107	33	0.013937832621351731
1107	34	0.1595234469137523
1107	35	0.07562588945983606
1107	36	-0.5474508489535808
1107	37	-0.13358936556088707
1107	38	-0.04333142187967809
1107	39	0.06995440896196925
1107	40	0.21916195687402507
1107	41	-0.22548439549314025
1107	42	-0.5060338241282096
1107	43	-0.3765668601592367
1113	1	-5.893497795401544
1113	2	-1.2939028478950974
1113	3	0.5614584602537427
1113	4	3.179540977025475
1113	5	-23.390005616256897
1113	6	-10.514305317398655
1113	7	-0.23902423150895657
1113	8	2.5228312551103187
1113	9	-20.60212719747627
1113	10	-21.23559616236746
1113	11	-9.470764098242446
1113	12	-21.68545659319753
1113	13	-10.379150167319187
1113	14	-0.3478365471467235
1113	15	1.4439815030451584
1113	16	0.6491282487026737
1113	17	-1.2466753517492393
1113	18	-0.7081754516856904
1113	19	-0.21202730607917886
1113	20	0.5906854497175829
1113	21	0.5776712211166384
1113	22	0.4322948491113294
1113	23	2.3720111006962483
1113	24	0.7312132567684344
1113	25	0.3585684962461144
1113	26	13.467439854448273
1113	27	7.190061456504466
1113	28	3.115265280471
1113	29	1.9083720263390447
1113	30	1.5743509999184795
1113	31	0.1451004633065054
1113	32	0.3639652570415066
1113	33	1.284788599706431
1113	34	0.425632233524631
1113	35	0.0881781907150696
1113	36	-0.1530296495114598
1113	37	0.7545556832536207
1113	38	0.7611316185666297
1113	39	-0.4374375017772252
1113	40	0.2568188606397186
1113	41	0.8978070968360043
1113	42	0.7023343767086132
1113	43	-0.3101791335204638
1114	1	-1.1242670261707737
1114	2	-0.48804204203429435
1114	3	-8.944036045240765
1114	4	-2.2614846640001645
1114	5	-0.7086869349382141
1114	6	-1.56925037234371
1114	7	0.8664702739855521
1114	8	1.5755785078575713
1114	9	-3.7230063183553916
1114	10	0.8083598815885829
1114	11	0.8398586123802652
1114	12	-21.68545659319753
1114	13	-8.628234416403437
1114	14	-0.3419757412859177
1114	15	-27.356018496954842
1114	16	-13.748673949099523
1114	17	-6.289166194240082
1114	18	-2.6195307630409985
1114	19	-1.8537855478374183
1114	20	-0.5433804843483543
1114	21	-1.1278232843778682
1114	22	-0.4973754805590005
1114	23	0.8467363754215285
1114	24	-0.5508380252828431
1114	25	-0.0011384634608475608
1114	26	11.60150578851421
1114	27	6.632552298995307
1114	28	2.3680125332182556
1114	29	0.8702767882438067
1114	30	0.34577957134705173
1114	31	0.2146975329035783
1114	32	0.3068223998986497
1114	33	-0.15770224278440992
1114	34	-0.4908512829588858
1114	35	-0.3513822488453684
1114	36	0.09165899517718401
1114	37	-0.5282281995302611
1114	38	-0.6535203960853835
1114	39	-0.4513569156966355
1114	40	-0.5234009195800624
1114	41	-0.2618998628709548
1114	42	-0.46469859032435323
1114	43	-0.6852707086120375
1115	1	-1.8649263668301153
1115	2	-0.9034266574189083
1115	3	-1.0385415397462587
1115	4	0.41763621512071225
1115	5	2.895708669457388
1115	6	1.1999803968870602
1115	7	2.8181186256339004
1115	8	3.0942598265388916
1115	9	-20.60212719747627
1115	10	-21.23559616236746
1115	11	-8.089811717290065
1115	12	-21.68545659319753
1115	13	-8.47438826255728
1115	14	-0.27164607095624405
1115	15	1.243981503045159
1115	16	-27.550871751297326
1115	17	-15.370484875558763
1115	18	-4.384365927876164
1115	19	-1.5263130203648885
1115	20	-1.3236002645681353
1115	21	-0.3651859217405047
1115	22	-0.8534194366029553
1115	23	0.5434396721248191
1115	24	0.40740373295891175
1115	25	-0.11762197994436008
1115	26	12.096011283019706
1115	27	7.256728123171129
1115	28	2.8866938518995724
1115	29	1.584562502529522
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2206	5	2.297445054619189
2206	6	-0.7463495162936837
2206	7	0.4383238347893865
2206	8	-0.28016795562369623
2206	9	1.9898222895008395
2206	10	1.5528805542860518
2206	11	0.9229743916286424
2206	12	2.698126674363639
2206	13	1.6187451233937828
2206	14	0.32756466306111776
2206	15	1.9218031289330817
2206	16	1.7979838130278516
2206	17	1.0839744772431317
2206	18	0.46346359067158716
2206	19	0.2924715100218478
2206	20	0.2470390408778016
2206	21	0.2237643545025847
2206	22	0.22456004248149242
2206	23	1.0408350943821247
2206	24	0.7819893683180297
2206	25	0.13273318974782455
2206	26	-7.723246806956617
2206	27	-4.444613628999349
2206	28	-1.7442453746197657
2206	29	-0.7011517831847698
2206	30	-0.24222358571687863
2206	31	-0.027485698229931188
2206	32	0.014004720340636823
2206	33	0.004704411334948588
2206	34	0.10298029982297408
2206	35	0.13079855377742433
2206	36	0.13494719868638327
2206	37	0.1514512370552481
2206	38	0.14460965066344755
2206	39	0.09936860187971064
2206	40	0.2749719782403517
2206	41	0.0692882859967554
2206	42	0.019303595335287582
2206	43	-0.02046590016082206
2207	1	-0.48233028271118883
2207	2	-4.73214311862268
2207	3	-1.3116379864467653
2207	4	-1.4732509011471144
2207	5	1.1988268710527485
2207	6	-2.580295165114393
2207	7	-0.33783979834482736
2207	8	0.2754539285451756
2207	9	0.858109689156553
2207	10	1.0588200812873616
2207	11	1.1055472384749834
2207	12	1.4702117655165168
2207	13	-1.1557999135120802
2207	14	0.18380473880928605
2207	15	1.8182623829097935
2207	16	1.62848527069929
2207	17	0.8054397074724164
2207	18	0.1622137192110955
2207	19	0.056331407964812286
2207	20	0.14026243787324688
2207	21	0.11709592500834276
2207	22	0.06241329242774185
2207	23	0.8884239602563184
2207	24	0.33019802834203915
2207	25	0.16499827627995245
2207	26	5.67082394920293
2207	27	4.030332184084834
2207	28	1.7277864310632154
2207	29	0.4400133122950507
2207	30	0.2887334026257591
2207	31	0.16202093707977383
2207	32	0.09628336194844422
2207	33	0.03504240681303372
2207	34	-0.20617825716910687
2207	35	0.03403267464062054
2207	36	0.040878979930162984
2207	37	-0.2731938937345362
2207	38	-0.24123724776163158
2207	39	-0.1017353021156353
2207	40	-0.20172597861578367
2207	41	0.04806090394260565
2207	42	-0.18802095324063117
2207	43	0.07086993585348011
2208	1	-23.893497795401544
2208	2	-9.899031053023304
2208	3	-1.192387693592412
2208	4	3.753899951384451
2208	5	4.363840537589258
2208	6	3.0626177595244215
2208	7	-23.639024231508955
2208	8	-7.631014898735835
2208	9	3.944026648677575
2208	10	3.402865376094077
2208	11	-21.20409743157578
2208	12	-6.700841208582144
2208	13	2.0772600890910695
2208	14	1.2239583246481516
2208	15	2.7670584261220803
2208	16	2.3183590179334423
2208	17	1.802042596968711
2208	18	1.0046450611348234
2208	19	0.7725880785362094
2208	20	0.8829931420252706
2208	21	0.6930558365012516
2208	22	0.832294849111328
2208	23	1.8412418699270177
2208	24	1.656854282409462
2208	25	1.2636967013743217
2208	26	-22.332560145551724
2208	27	-12.981733415290407
2208	28	-3.715503950298231
2208	29	-0.858294640327621
2208	30	0.1358894614569408
2208	31	0.8784337966398397
2208	32	1.01781141088766
2208	33	1.2411988561166893
2208	34	1.3179399258323272
2208	35	1.2651012676381441
2208	36	1.1597908633090555
2208	37	1.2904531191510529
2208	38	1.0047213621563742
2208	39	1.3266650623253398
2208	40	1.4029727067935625
2208	41	0.995242994271905
2208	42	1.3792574536316877
2208	43	1.4334106100692807
2209	1	-2.576424624669837
2209	2	-3.8060979698463164
2209	3	-4.267809832429183
2209	4	-2.392816746551759
2209	5	-2.2111438276390096
2209	6	-8.140321577561256
2209	7	-4.43577219898863
2209	8	-5.799119964401875
2209	9	-2.7647288234925327
2209	10	-0.8534823412292489
2209	11	-2.854503935640821
2209	12	-1.9374891135227337
2209	13	-2.330369679514309
2209	14	-0.35108857966704576
2209	15	1.3431684949150764
2209	16	1.2783965413856002
2209	17	0.04112952629954236
2209	18	-0.2512648825799957
2209	19	-1.1973931597377145
2209	20	-1.0971194283311974
2209	21	-0.5247678032736118
2209	22	-1.1595750695878593
2209	23	-0.3304279236939962
2209	24	-0.34195747493888007
2209	25	-1.2446835362742092
2209	26	-3.4057308772590424
2209	27	-2.648962933739437
2209	28	-2.3562794349761518
2209	29	-1.4851239086203094
2209	30	-1.6809335529270442
2209	31	-1.602867016368286
2209	32	-0.4945713283243478
2209	33	-0.7217154653342206
2209	34	-1.0963189859875655
2209	35	-2.0825535166020046
2209	36	-1.3660377795927587
2209	37	-1.5397532598358126
2209	38	-1.5413074058236163
2209	39	-1.8016651440536435
2209	40	-1.6537502450513415
2209	41	-2.6517863990989525
2209	42	-2.0017306639417995
2209	43	-0.6142441741708708
2215	1	-4.593497795401543
2215	2	-0.6855695145617666
2215	3	-0.9635415397462559
2215	4	0.9462076436921407
2215	5	2.834994383743105
2215	6	2.310694682601344
2215	7	1.8609757684910448
2215	8	0.472831255110318
2215	9	-20.60212719747627
2215	10	-9.885596162367461
2215	11	-2.7040974315757786
2215	12	-21.68545659319753
2215	13	-11.920816833985853
2215	14	0.21049678618661005
2215	15	-27.356018496954842
2215	16	-13.850871751297326
2215	17	-3.963342018415908
2215	18	-1.9915087850190218
2215	19	-1.4370273060791803
2215	20	-0.43431455028242283
2215	21	-0.27232877888336304
2215	22	-0.292705150888672
2215	23	0.44701110069625116
2215	24	-0.8104534098982299
2215	25	0.47523516291278156
2215	26	8.692439854448274
2215	27	5.381728123171129
2215	28	2.2152652804710016
2215	29	0.11670535967237328
2215	30	-6.490000815162489E-4
2215	31	-0.12156620336016033
2215	32	0.16396525704150378
2215	33	-0.5818780669602361
2215	34	-0.27436776647536476
2215	35	-0.11182180928493324
2215	36	-0.2613629828447941
2215	37	0.8462223499202892
2215	38	-0.322201714766706
2215	39	0.5958958315561063
2215	40	-0.06818113936028425
2215	41	0.41447376350267007
2215	42	0.7023343767086132
2215	43	0.30648753314620336
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	11.0
25	131.0
26	1092.0
27	3982.0
28	9793.0
29	18221.0
30	29452.0
31	40114.0
32	49620.0
33	53346.0
34	43335.0
35	31129.0
36	13367.0
37	8460.0
38	404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.9905165429212767	1.298330433396645	96.54398384890223	1.1671691747798483
2	96.99659786349795	1.5618749111443941	0.47180260334526897	0.9697246220123853
3	1.0457684894051056	2.1560089533388216	0.6814191769408544	96.11680338031522
4	2.384831479507345	90.71679036680948	0.9061286883762093	5.992249465306971
5	1.0289770576984054	2.270022945711061	2.061704528794651	94.63929546779588
6	94.3687490701096	2.8602602039972886	0.656626605610752	2.114364120282356
7	3.1678249926117292	2.662434045153128	91.30375589816472	2.8659850640704225
8	5.823307114730358	83.12751895310738	3.5000016531275517	7.549172279034706
9	26.749070878683302	20.14135917175471	25.36534377488718	27.744226174674807
10	24.041765662952027	23.93771601072308	23.189486345968408	28.831031980356485
11	21.511253164641076	23.86322105640869	25.232405924606788	29.393119854343446
12	22.182543116359255	24.29692544247935	27.85473231580279	25.665799125358607
13	24.91551335693595	22.068782855916748	30.74073329041948	22.274970496727818
14	29.173212330873817	24.553599481418033	20.813062444809717	25.460125742898438
15	26.398089943980104	28.350318619393445	23.280634117346246	21.97095731928021
16	27.186873133883704	23.166323510913678	29.6089348489563	20.03786850624632
17	22.89350221684405	25.530571287819427	29.777455968947653	21.798470526388876
18	20.528634069880493	25.69956547906429	27.33348324415844	26.438317206896777
19	21.448007485361558	28.0935802444645	21.284678483222407	29.173733786951534
20	25.417827988771958	26.307541237266786	23.518384431505964	24.75624634245529
21	22.620736170761464	30.16759407122335	23.0363324373382	24.17533732067699
22	22.694796285091765	25.172503860052835	24.442152107572316	27.690547747283084
23	24.657557073821028	26.02266288951841	26.21796367272121	23.101816363939342
24	23.38778735489673	30.243307313105667	23.913151290927306	22.4557540410703
25	20.743113897182077	28.032745150550326	28.922458399045155	22.30168255322244
26	18.614572807903894	27.853475815752677	27.502373005909554	26.029578370433875
27	19.697210154271396	29.18857082399307	29.11847752054858	21.99574150118696
28	21.6717252871511	30.509432839373922	27.134374152763723	20.68446772071125
29	20.979196698980335	30.714295160885037	27.18480949043154	21.121698649703095
30	20.689883190007173	27.576481946194004	27.79700916163289	23.93662570216593
31	21.329312927126832	29.709016488294203	28.775991297936564	20.1856792866424
32	19.66725848633029	29.91334305372334	30.479043302023097	19.940355157923275
33	22.971199211788786	29.87333736696456	27.77882475856072	19.376638662685934
34	20.784442085982473	30.37159001114208	26.632215488482657	22.21175241439279
35	19.796533060897914	33.27844949860641	25.57752011029667	21.347497330199
36	19.98432835080689	31.853122923258514	25.461470556145173	22.701078169789422
37	21.74722357227639	31.785675319136274	24.544315390286883	21.922785718300453
38	21.635472149760133	31.350241521935352	25.181100123323315	21.833186204981203
39	22.387645185927255	32.795075002397034	24.584982328066467	20.23229748360924
40	22.236549327673025	30.703868649097227	27.006152940748603	20.05342908248115
41	23.546156974379826	32.30740237455241	25.52660378169459	18.619836869373167
42	21.710854766132044	34.05145194192893	25.55900508171409	18.678688210224927
43	19.342914860624816	33.455664772182494	27.9563706576472	19.24504970954549
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	4.0
9	7.0
10	31.0
11	55.0
12	55.0
13	127.0
14	199.0
15	421.0
16	643.0
17	1018.5
18	1394.0
19	1394.0
20	1793.5
21	2193.0
22	2621.0
23	3049.0
24	3603.5
25	4158.0
26	4158.0
27	5182.0
28	6206.0
29	7241.0
30	8276.0
31	9259.5
32	10243.0
33	10243.0
34	11058.0
35	11873.0
36	13167.5
37	14462.0
38	16908.5
39	19355.0
40	19355.0
41	20932.5
42	22510.0
43	23788.5
44	25067.0
45	26650.0
46	28233.0
47	28233.0
48	29972.0
49	31711.0
50	30426.5
51	29142.0
52	27740.0
53	26338.0
54	26338.0
55	23439.5
56	20541.0
57	17806.5
58	15072.0
59	11579.0
60	8086.0
61	8086.0
62	6422.5
63	4759.0
64	4118.5
65	3478.0
66	3046.5
67	2615.0
68	2615.0
69	2112.5
70	1610.0
71	1117.5
72	625.0
73	456.0
74	287.0
75	287.0
76	216.5
77	146.0
78	113.5
79	81.0
80	55.5
81	30.0
82	30.0
83	17.0
84	4.0
85	4.0
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.4301437890344743
2	0.0
3	0.0
4	1.3737489957250122
5	3.0272071732510737
6	6.612510208062634E-4
7	0.4311356655656838
8	0.0
9	0.3626961849122355
10	0.2241640960533233
11	0.4876726278446192
12	0.6582753912126352
13	1.3836677610371062
14	0.03107879797789438
15	0.43444192066971504
16	0.11869455823472427
17	0.0
18	0.018184403072172242
19	0.0
20	0.0
21	0.0
22	0.0
23	0.7958156035403379
24	0.0
25	0.0
26	35.21260873446474
27	9.91876531209395E-4
28	9.91876531209395E-4
29	0.0016531275520156583
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	302457.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.280232792195584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.50444715771795	43.698001325279385
2	9.094997231290968	9.873571339176962
3	3.86015563541017	6.28590439512509
4	2.0829325855470424	4.522482625357731
5	1.2107728796062982	3.2860516881753425
6	0.7169373865812103	2.3349316944633847
7	0.4818492203095947	1.8308421494399911
8	0.321698332655395	1.396948830831681
9	0.235934305050234	1.1525912101612428
>10	1.2887947667047408	13.298116577854183
>50	0.13036973472883578	4.858406317939236
>100	0.06861564985728198	6.714548448745765
>500	0.002495114540264799	0.7476033974499849
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCACGTACGCATGGCAAGGATAGACAATTTAGGAATCGGAGA	625	0.2066409440019573	No Hit
TGCACGTACGCATGGGCAAGGATAGACAATTTAGGAATCGGAG	562	0.18581153684656002	No Hit
TGCACGTAGTCCAAATTTAACGTCCGAAACTCGAATAGCCATT	517	0.17093338887841908	No Hit
TGCACGTATAAAAGTTCTACAGTGAGTCGTATTACCGGCCGAC	504	0.16663525724317838	No Hit
TGCACGTACGTTGACCAGCTGTCAGCACTGTACAGGGAGCACC	464	0.15341023682705313	No Hit
TGCACGTATAAAACCGGCCGACGATCTAAAATACGTGCATTTT	428	0.14150771845254037	No Hit
TGCACGTACGCATGGCAAGGATAGANAATTTAGGAATCGGAGA	408	0.1348952082444777	No Hit
TGCACGTAGCGCCGTGGTTTGGCGCCTGCCTTTGGCCCAGGGC	407	0.1345645827340746	No Hit
TGCACGTACGCATGGGCAAGGATAGNCAATTTAGGAATCGGAG	399	0.13191957865084955	No Hit
TGCACGTCCCCATATGATAGTTAGATGATAAGAATGGTACTCA	397	0.13125832763004328	No Hit
TGCACGTAGTCCAAATTTAACGTCCNAAACTCGAATAGCCATT	369	0.12200081333875558	No Hit
TGCACGTATAAAAGTTCTACAGTGANTCGTATTACCGGCCGAC	339	0.11208204802666165	No Hit
TGCACGTAACTCTAGGTGACGTAGGGTGTTTTTTGCCGTTTTT	327	0.10811454190182407	No Hit
TGCACGTACGTTGACCAGCTGTCAGNACTGTACAGGGAGCACC	322	0.10646141434980841	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	6.612510208062634E-4	0.0	0.0
12	0.0	0.0	0.0026450040832250534	0.0	0.0
13	0.0	0.0	0.009257514291287686	0.0	0.0
14	0.0	0.0	0.07042323371586705	6.612510208062634E-4	0.0
15	0.0	0.0	0.11307392455787103	9.91876531209395E-4	0.0
16	0.0	0.0	0.14448334804616855	9.91876531209395E-4	0.0
17	0.0	0.0	0.18415840929454436	9.91876531209395E-4	0.0
18	0.0	0.0	0.22383347054292016	9.91876531209395E-4	0.0
19	0.0	0.0	0.28632169200911206	9.91876531209395E-4	0.0
20	0.0	0.0	0.3617043083810261	9.91876531209395E-4	0.0
21	0.0	0.0	0.4096450073894802	9.91876531209395E-4	0.0
22	0.0	0.0	0.4757701094701065	9.91876531209395E-4	0.0
23	0.0	0.0	0.5957871697464433	0.00793501224967516	0.0
24	0.0	0.0	0.8262331504974261	0.00793501224967516	0.0
25	0.0	0.0	1.0431234853218805	0.00793501224967516	0.0
26	0.0	0.0	1.2180243803251372	0.008596263270481424	0.0
27	0.0	0.0	1.5830349438101945	0.010580016332900214	0.0
28	0.0	0.0	1.8686953847985004	0.010910641843303346	0.0
29	0.0	0.0	2.066409440019573	0.011571892864109609	0.0
30	0.0	0.0	2.350416753455863	0.013886271436931531	0.0
31	0.0	0.0	2.636407819954572	0.01587002449935032	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAT	20	3.2378398E-4	52.643787	24
AGTGCCC	35	1.9437866E-6	45.68277	20
TCCGAAT	30	4.1909785E-5	44.413803	22
CCGAATT	30	4.1909785E-5	44.413803	23
ACGCCTA	25	9.153752E-4	42.637253	23
TCCCTTG	25	9.726426E-4	42.115032	26
TCCGAAA	110	0.0	41.18371	23
AAGCCGC	40	4.869551E-6	39.97242	21
TCCGTGA	75	7.2759576E-12	38.605442	25
ATCCGAA	35	1.0400098E-4	38.068974	21
CCGAAAC	120	0.0	37.28935	24
GTTGTCT	30	0.0022425405	35.53104	22
TCGCTTT	45	1.0925845E-5	35.53104	23
AAGCTCC	30	0.0022425405	35.53104	22
CGTATCC	385	0.0	35.448627	5
CGTATAC	220	0.0	35.448627	5
GGTAAGC	30	1.112033E-5	35.448627	5
CGCGAGG	25	1.5841836E-4	35.448627	5
CGTAGAC	370	0.0	35.448627	5
CGAATAA	25	1.5841836E-4	35.448627	5
>>END_MODULE