FastQCFastQC Report
Thu 26 May 2016
SRR2099042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116122
Sequences flagged as poor quality0
Sequence length43
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGATCGCAGTGACGATCTAGCACTGGATCGTCGGACTGTAG25490.22838005164309993No Hit
CTAGATCGGAGGTGAGTCGTATTACCGGCCGACGATCTAGACC23650.21189439864100876No Hit
CTAGATCGGAGGTCGACTCACTATAGTGAGTCGTATTACCGGC18820.16861955951051946No Hit
CTAGATCTCTTATATTTTAATCCCCAATATGGGGATAAGGGGC14770.13233320371787313No Hit
CTAGATCGCAGTCCGACGATCTAGGACTGGATCGTCGGACTGT14060.12597189196163142No Hit
CTAGATCGGACCAGTTCTATGCCCCTGTAAAGGGGAATGCCGA12700.11378684409052058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAT2800.046.03195624
CTACGCC259.776548E-442.08635725
TAACTAC1100.040.65159624
CCCCAAT3200.039.9585522
CCCAATA3250.039.34380323
CCAGCGT1650.038.74768422
CAATATG3950.037.95763425
CCCTTGC505.288348E-737.29464721
AGCGTCC1700.037.1350224
CAGCGTC1750.036.53352723
ATCCTCG200.002240102535.5535475
ATAGCGT251.5596792E-435.5535475
ATATCGC650.035.5535473
AGATCGC257200.035.52593
TTTATCG200.00226692535.467412
TAGCACG552.0008883E-1135.4674072
GATCGCA118850.035.4039734
ATCGCAG78150.035.3943185
AGATCGA149250.035.255783
ATCGCAT17350.035.246175
Produced by FastQC (version 0.11.5)