FastQCFastQC Report
Thu 26 May 2016
SRR2099040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181809
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGTCGAGTCATGTCCGACGATCGTCGGACTGTAGAACTCTG17640.1492626981178854Illumina DpnII expression Sequencing Primer (100% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCCA652.7703209E-936.78532820
ATTCGAC192650.035.395843
ATTCGAG250150.035.3681223
TTCGACC39700.035.2816164
TTCGAGT75350.035.272024
TTCGAGC70950.035.2603454
TTCGCGT7150.035.2122844
TTCGACG59500.035.072884
TTCGACT57800.035.0614854
TCGAGCG15800.035.0113955
ACTATCG200.002448231434.91568411
CTCGCGA200.002448231434.91568410
ACGCGCC503.3469405E-1034.9141738
TCGAGGG19100.034.9032945
GCGTTCG28850.034.9011421
CTTCGCG3050.034.8789443
TCGATAC14950.034.867285
TTCGAGA47550.034.8636634
TTCGATA48550.034.8029144
TTCGATG61000.034.7917444
Produced by FastQC (version 0.11.5)