##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1181809
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.825645260782412	30.0	26.0	31.0	17.0	35.0
2	28.788244124050504	30.0	27.0	31.0	21.0	35.0
3	23.289557788102815	26.0	17.0	30.0	8.0	32.0
4	26.690488056868748	29.0	24.0	31.0	15.0	35.0
5	27.4934553722302	30.0	26.0	31.0	13.0	37.0
6	29.183333347436008	30.0	27.0	32.0	17.0	37.0
7	28.34586468710257	30.0	26.0	31.0	17.0	38.0
8	22.138219458474254	25.0	17.0	29.0	8.0	32.0
9	25.814444635300628	27.0	26.0	28.0	20.0	31.0
10	25.846788271201184	27.0	26.0	27.0	20.0	31.0
11	25.414246295298142	27.0	26.0	27.0	16.0	31.0
12	27.664905242725347	30.0	26.0	31.0	17.0	35.0
13	27.145877210276787	30.0	25.0	31.0	16.0	35.0
14	31.18416004616651	31.0	31.0	33.0	30.0	34.0
15	31.151598100877553	31.0	31.0	34.0	30.0	34.0
16	31.38801532227289	31.0	31.0	34.0	30.0	34.0
17	35.29292719889593	35.0	35.0	37.0	33.0	37.0
18	35.35900809690906	35.0	35.0	37.0	33.0	37.0
19	34.9527614022232	35.0	35.0	37.0	32.0	37.0
20	34.88412594590158	35.0	35.0	37.0	32.0	37.0
21	34.855848110819935	35.0	35.0	37.0	32.0	37.0
22	36.35126742138535	37.0	35.0	39.0	32.0	39.0
23	36.20559498193025	37.0	35.0	39.0	32.0	39.0
24	36.47013688337117	37.0	35.0	39.0	32.0	39.0
25	36.60774964482416	37.0	35.0	39.0	32.0	39.0
26	22.73095144816125	32.0	2.0	37.0	2.0	39.0
27	29.59229790939145	34.0	17.0	38.0	17.0	40.0
28	34.391349194328356	36.0	32.0	39.0	27.0	40.0
29	36.54391868736826	37.0	35.0	39.0	32.0	40.0
30	37.41556461323277	38.0	36.0	40.0	33.0	41.0
31	37.84605803475858	39.0	37.0	40.0	34.0	41.0
32	38.22305042523792	39.0	38.0	40.0	34.0	41.0
33	38.31759615978555	40.0	38.0	40.0	34.0	41.0
34	38.307350849418135	40.0	38.0	40.0	34.0	41.0
35	38.279815943185405	40.0	38.0	40.0	34.0	41.0
36	38.19694383779443	40.0	38.0	40.0	34.0	41.0
37	38.1693463156906	40.0	37.0	40.0	34.0	41.0
38	38.09933161788411	40.0	37.0	40.0	34.0	41.0
39	37.96908806753037	39.0	37.0	40.0	34.0	41.0
40	37.8516511551359	39.0	37.0	40.0	33.0	41.0
41	37.78739457898865	39.0	37.0	40.0	33.0	41.0
42	37.67162714110317	39.0	37.0	40.0	33.0	41.0
43	37.564217229687706	39.0	37.0	40.0	33.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.0753134561536655
1101	2	5.496713836564044
1101	3	4.524106304182524
1101	4	7.1431466712227305
1101	5	8.67173107134434
1101	6	6.644900205647762
1101	7	6.951171290382398
1101	8	5.009020763842681
1101	9	5.923688195763464
1101	10	5.551822582519087
1101	11	3.4210644488615713
1101	12	7.767337937765188
1101	13	6.621468782699541
1101	14	0.1779423439918304
1101	15	1.065330394919311
1101	16	1.568160818536704
1101	17	0.8820156910672452
1101	18	0.34515533284805855
1101	19	0.21600800552416644
1101	20	0.18226247609991475
1101	21	0.07266419672928492
1101	22	0.021112722640985737
1101	23	1.0510718069345657
1101	24	0.5564477033499031
1101	25	0.308718036969438
1101	26	12.258392661684443
1101	27	7.271338369915679
1101	28	3.209291905691572
1101	29	1.4570069128841254
1101	30	0.7474317530737622
1101	31	0.42580280431306505
1101	32	0.129649622581141
1101	33	-0.00178984306834451
1101	34	0.13696316745731707
1101	35	0.10327480087062924
1101	36	0.05461526390827487
1101	37	-0.11774428609666643
1101	38	-0.12085769607686103
1101	39	-0.10318281741528779
1101	40	-0.18188636181891837
1101	41	-0.22143056812800666
1101	42	-0.17843095112949214
1101	43	-0.24558843118645513
1102	1	3.380728645920719
1102	2	2.5171736588890425
1102	3	0.5901389113748969
1102	4	3.8399720802704103
1102	5	5.051530381450874
1102	6	3.3147542144293887
1102	7	3.557673463811465
1102	8	1.4311344346390094
1102	9	4.250192816757892
1102	10	3.5105665658367684
1102	11	3.715896497584417
1102	12	5.399471891806282
1102	13	4.017211282376962
1102	14	-0.16357421056577692
1102	15	1.2669813353181816
1102	16	1.7139044395788297
1102	17	0.894243500343741
1102	18	0.42454115093369893
1102	19	0.24616135289499397
1102	20	0.11872176555240088
1102	21	0.10686088619155498
1102	22	0.14456279103675485
1102	23	0.9447641155239666
1102	24	0.4535014147755234
1102	25	0.2644864790454946
1102	26	12.133847244245967
1102	27	7.133376833024055
1102	28	3.0052365724735637
1102	29	1.3488139083757886
1102	30	0.7042978616795779
1102	31	0.39733542877445416
1102	32	0.0868321055487371
1102	33	0.06122898434600188
1102	34	0.06480503081260025
1102	35	0.02887595327470649
1102	36	-0.019725384859512474
1102	37	-0.06794272279465474
1102	38	-0.18005599656641635
1102	39	-0.18724738021238352
1102	40	-0.16030356750067654
1102	41	-0.15157519128143804
1102	42	-0.06514347463516401
1102	43	-0.094379727123723
1103	1	-2.354721284221103
1103	2	-0.6308204878172177
1103	3	-0.8282693777929779
1103	4	1.3820242306347907
1103	5	2.0179238411347704
1103	6	-0.1829199673341897
1103	7	0.12042827703794501
1103	8	-1.4238173931400517
1103	9	2.3449323232569874
1103	10	1.4428642549602628
1103	11	-1.1512447363574019
1103	12	1.8173316669889559
1103	13	1.442696544884992
1103	14	-0.441674610833239
1103	15	1.4872123486315623
1103	16	1.781226609727998
1103	17	0.9675624346042042
1103	18	0.4280214224569505
1103	19	0.20877301168380313
1103	20	0.17901370173707676
1103	21	0.12658267261323175
1103	22	0.20032853440097398
1103	23	0.9405202173819802
1103	24	0.5121326628450831
1103	25	0.3785082545768077
1103	26	12.204608428894932
1103	27	7.329216582567906
1103	28	3.198980297967914
1103	29	1.4704883042298675
1103	30	0.6756948505115616
1103	31	0.4056965734301272
1103	32	0.136712776112887
1103	33	0.08205263064173351
1103	34	0.2345112436371437
1103	35	0.20179482449741215
1103	36	0.2011885065289576
1103	37	0.0947011053218958
1103	38	0.04613870090251737
1103	39	0.09748508657478538
1103	40	-0.017458650421716015
1103	41	0.060851740114081565
1103	42	0.13846727687080573
1103	43	-0.06626957012773715
1104	1	1.1581933941657674
1104	2	-0.5869270696506135
1104	3	-1.5602670401752547
1104	4	1.681563761235875
1104	5	0.9821897181959223
1104	6	-0.7512881168912529
1104	7	-0.07066566325014634
1104	8	-0.24430601603603108
1104	9	2.11430821201483
1104	10	1.0568383169237663
1104	11	0.6541339637355925
1104	12	2.7194893753206735
1104	13	1.0012250214103169
1104	14	0.2787307387326585
1104	15	1.8853553591058194
1104	16	1.8197419586601704
1104	17	0.8252654040267728
1104	18	0.19624599434055767
1104	19	0.060820477481946966
1104	20	-0.04681029273889692
1104	21	-0.0661092037350457
1104	22	-0.17745437627146288
1104	23	0.7879128072599713
1104	24	0.3134965217036694
1104	25	0.1436358844325909
1104	26	11.985836429494821
1104	27	6.973965215540414
1104	28	2.789297594016361
1104	29	1.0950990716664393
1104	30	0.37671162245992207
1104	31	0.13551433272948543
1104	32	-0.07874377479409844
1104	33	-0.14011037690190165
1104	34	-0.10135541695034078
1104	35	-0.21051067392242828
1104	36	-0.24154401718344332
1104	37	-0.365780624010533
1104	38	-0.43310877659518354
1104	39	-0.3687421871039902
1104	40	-0.3355481468847543
1104	41	-0.3721163834630872
1104	42	-0.2671578595165087
1104	43	-0.2725373793366117
1105	1	1.3654908754974677
1105	2	-1.4421161669965201
1105	3	-2.2817733075902034
1105	4	0.13797018592905985
1105	5	-1.4980782387290503
1105	6	-1.5086374721553568
1105	7	-6.006319917115189
1105	8	-2.3101975171720603
1105	9	1.7326096404367881
1105	10	0.8728346503555322
1105	11	1.1505596078816112
1105	12	3.038033740105128
1105	13	0.6147428462373021
1105	14	0.2715808353423448
1105	15	1.8396396597157967
1105	16	1.701840339664372
1105	17	0.8349116912570196
1105	18	0.24965257323743373
1105	19	-0.04630771345607343
1105	20	0.019171347249582027
1105	21	-0.044109222325289466
1105	22	-0.16139159463223507
1105	23	0.7372665460083994
1105	24	0.29583521736920915
1105	25	0.22544173125517375
1105	26	12.123666456982168
1105	27	6.936338131556123
1105	28	2.8237788749509463
1105	29	1.1498696978406926
1105	30	0.42475582148135516
1105	31	0.23136253527925987
1105	32	0.031543566035871606
1105	33	-0.07429047204104933
1105	34	0.05911028297594356
1105	35	-0.13195487852094345
1105	36	-0.195663913297345
1105	37	-0.18517349045702503
1105	38	-0.19093923785490574
1105	39	-0.22362054136207377
1105	40	-0.25757813485591186
1105	41	-0.23795182786168567
1105	42	-0.23799419886855588
1105	43	-0.27044392713057874
1106	1	-4.482588722765826
1106	2	-1.5575796942477567
1106	3	-4.658700698372314
1106	4	0.3762244448838459
1106	5	0.9219208014308578
1106	6	-0.8996979377180736
1106	7	-4.297207577900988
1106	8	-1.9353073366382212
1106	9	-0.4259657420813028
1106	10	0.2857398681603627
1106	11	0.8476311223448221
1106	12	3.005641687715311
1106	13	-4.356908511651156
1106	14	0.14012080110357417
1106	15	1.8606014933191268
1106	16	1.6890946701037528
1106	17	0.7685898286839716
1106	18	0.20231896537641347
1106	19	-0.0316496799804753
1106	20	-0.0942202678424664
1106	21	-0.11796146668983454
1106	22	-0.14179098879191088
1106	23	0.7824820619912529
1106	24	0.3683285832297116
1106	25	0.15909275152156255
1106	26	11.956590717208908
1106	27	6.956601698274913
1106	28	2.776893505699455
1106	29	1.106415924712664
1106	30	0.38185808438826996
1106	31	0.18350645222557205
1106	32	-0.18856421125303768
1106	33	-0.2429362551379981
1106	34	-0.14964607638415828
1106	35	-0.18735168010555014
1106	36	-0.2578564498358702
1106	37	-0.2234393614632424
1106	38	-0.24598177118836873
1106	39	-0.23563767215841125
1106	40	-0.19413780620129728
1106	41	-0.1818208492912916
1106	42	-0.18310537419274198
1106	43	-0.2089551614547247
1107	1	1.892372561069081
1107	2	0.3258241183085637
1107	3	-1.447512846577382
1107	4	-22.549544677328967
1107	5	-23.144689415899087
1107	6	-11.051331464446722
1107	7	-6.039482552129144
1107	8	-2.6992523702646025
1107	9	1.0245873597327986
1107	10	1.5088288011497255
1107	11	2.3597425831266854
1107	12	3.89262841167778
1107	13	2.2505193306237885
1107	14	0.2052766372751762
1107	15	1.7172449124230198
1107	16	1.6578853873483652
1107	17	0.8359453751336119
1107	18	0.26965032710535297
1107	19	-0.02199970159556841
1107	20	0.12795569194054224
1107	21	0.06214764487620528
1107	22	-0.04106629913272286
1107	23	0.8596054873392021
1107	24	0.5338119849535872
1107	25	0.16029649102903676
1107	26	11.985989443828398
1107	27	6.969621872454365
1107	28	2.9360363882048617
1107	29	1.2308075621858947
1107	30	0.6085991850294121
1107	31	0.17522428469196427
1107	32	-0.033855927079272874
1107	33	-0.005152905713579514
1107	34	-0.22102453126083077
1107	35	-0.11862420040903743
1107	36	-0.15820468233128793
1107	37	-0.029777737036020824
1107	38	0.010874803249315335
1107	39	0.03880641592783718
1107	40	0.08040824246880618
1107	41	-0.07809368152270935
1107	42	-0.3209519611652638
1107	43	-0.08681545127012669
1113	1	-9.987512063501478
1113	2	-1.2798968862660338
1113	3	3.6019993799111987
1113	4	2.865549662293674
1113	5	-23.144689415899087
1113	6	-11.194981222856452
1113	7	-1.5995310539020338
1113	8	4.164336393491343
1113	9	-20.911147791192224
1113	10	-21.463907323485135
1113	11	-11.479503434767528
1113	12	-21.814284928146954
1113	13	-10.404475065951692
1113	14	0.17354839417841106
1113	15	2.0184519451800895
1113	16	1.2556329944105187
1113	17	0.7991097483768002
1113	18	-0.2189315471942166
1113	19	-0.7184410677288398
1113	20	0.21972325086836264
1113	21	0.10728736305150477
1113	22	0.23333615535093344
1113	23	0.9677423946163017
1113	24	1.1071066148519932
1113	25	0.47941611036360143
1113	26	12.509698359703545
1113	27	6.958966179926211
1113	28	2.960103555811557
1113	29	2.2292092083066706
1113	30	1.5667296621472317
1113	31	0.7265277698382988
1113	32	1.2009734899808606
1113	33	0.8463655304716795
1113	34	0.5557203383906568
1113	35	-0.033415403951138956
1113	36	0.1762922036344321
1113	37	0.6791748818760013
1113	38	0.8688233987188099
1113	39	1.0559657552749044
1113	40	1.0404677673718936
1113	41	0.555093289137389
1113	42	1.050839848648998
1113	43	0.7650887944721845
1114	1	-1.5047516602965167
1114	2	-1.3062602876617433
1114	3	-2.4315493406884414
1114	4	0.6559347747258286
1114	5	0.4498311320461177
1114	6	-1.7493880463250484
1114	7	1.2082745806288528
1114	8	-0.7740973103080862
1114	9	-4.861832722699074
1114	10	0.49499678610390774
1114	11	0.9109850635524523
1114	12	-21.814284928146954
1114	13	-8.67736211686924
1114	14	-0.24309673376162522
1114	15	-27.69852918689538
1114	16	-13.961994299463868
1114	17	-6.107326023140388
1114	18	-2.430252301911196
1114	19	-1.3917933551416084
1114	20	-0.737061448020242
1114	21	-0.49762346662024015
1114	22	-0.3695586495081997
1114	23	0.6272409730603457
1114	24	0.044454766829247205
1114	25	-0.09230784931590108
1114	26	11.878889365131304
1114	27	6.599286676178476
1114	28	2.3393229923584684
1114	29	0.895531255347251
1114	30	0.22426390872257684
1114	31	-0.12263221982311734
1114	32	-0.49176365139934575
1114	33	-0.49485959229905774
1114	34	-0.7143752935555838
1114	35	-0.4513528554889987
1114	36	-0.3569722057736797
1114	37	-0.4426653869273096
1114	38	-0.47519314302324744
1114	39	-0.4922896078680239
1114	40	-0.3784518501003191
1114	41	-0.16933162686157743
1114	42	-0.07834081818997873
1114	43	-0.556906553162861
1115	1	-2.1426706343565556
1115	2	-0.9101658545518632
1115	3	2.7776316561054983
1115	4	-2.836778719882158
1115	5	2.5574382436753815
1115	6	0.9676040842491282
1115	7	2.110023341922936
1115	8	4.302032098027677
1115	9	-20.911147791192224
1115	10	-21.463907323485135
1115	11	-8.383156586112369
1115	12	-21.814284928146954
1115	13	-8.27902344009862
1115	14	0.15066601762281095
1115	15	1.4184920897003614
1115	16	-27.83870662823099
1115	17	-15.463636136809875
1115	18	-4.860039535953749
1115	19	-1.9476662784875671
1115	20	-1.1361579213516322
1115	21	-0.7393605695056991
1115	22	0.07255735165764321
1115	23	0.6106568867881208
1115	24	0.6205550291434392
1115	25	0.7096449341291589
1115	26	12.110862550791463
1115	27	6.782731735927822
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2206	1	1.8558296095924476
2206	2	0.23474409644620664
2206	3	-0.6331254343253363
2206	4	1.9200465489920688
2206	5	2.3418509629643225
2206	6	-0.3348672252267022
2206	7	0.5679325928934311
2206	8	-0.6731699553865482
2206	9	2.1369080413102672
2206	10	1.5181465149994118
2206	11	1.2331773906806553
2206	12	3.0008696082438746
2206	13	1.7234054434634594
2206	14	0.29586446483437
2206	15	1.9742524681395146
2206	16	1.8199174993861575
2206	17	0.8093131005843262
2206	18	0.28619834614463713
2206	19	0.19457083994436886
2206	20	0.15556478287611242
2206	21	0.09838576595600301
2206	22	0.14376712658816615
2206	23	1.010425665558195
2206	24	0.6472409288910086
2206	25	0.2719855717906441
2206	26	-7.554539116546948
2206	27	-4.327762313837777
2206	28	-1.8038611538330969
2206	29	-0.6744082412315038
2206	30	-0.08511480445297082
2206	31	0.12756240179149358
2206	32	0.1930312788783155
2206	33	0.195420253096259
2206	34	0.21329102256454746
2206	35	0.22052466264153736
2206	36	0.24459296173748157
2206	37	0.2542684690390402
2206	38	0.16017199444107177
2206	39	0.10833318129871827
2206	40	0.16050388543281002
2206	41	0.26601712141414424
2206	42	0.21012802186520219
2206	43	0.23067317341084248
2207	1	0.31424471741283
2207	2	-5.382841647613603
2207	3	-2.0758970333954814
2207	4	-0.9298203017906381
2207	5	0.19854917152554563
2207	6	-1.0090484713247534
2207	7	-0.32588114247239375
2207	8	0.16108610502824305
2207	9	1.2154671958878787
2207	10	1.313870454292644
2207	11	1.4996297132993632
2207	12	1.4897633061338382
2207	13	-1.178092474496701
2207	14	0.4165318339586932
2207	15	2.0732193057833435
2207	16	1.856383811045493
2207	17	0.9578457111934995
2207	18	0.35906725019905394
2207	19	0.2063553829561613
2207	20	0.18941431094994243
2207	21	0.20146447126985834
2207	22	0.2565513406986355
2207	23	1.043335978546878
2207	24	0.6324669905853426
2207	25	0.3523135800140338
2207	26	6.781845012719
2207	27	4.335593355588056
2207	28	1.8594827507151024
2207	29	0.7338083664819592
2207	30	0.47715171210416685
2207	31	0.35232205908146597
2207	32	0.20510135632899562
2207	33	0.027999775510927805
2207	34	-0.012600725103730781
2207	35	0.18267189879698265
2207	36	0.06228021007000706
2207	37	-0.0824327107978533
2207	38	-0.07688865822624535
2207	39	-0.1310233400073031
2207	40	0.056613575312347564
2207	41	-0.0966545534836385
2207	42	0.02647081089689607
2207	43	0.06416408740443558
2208	1	-23.855436591803365
2208	2	-10.544810170047242
2208	3	2.2640920042679724
2208	4	3.455505827721538
2208	5	4.037128765919096
2208	6	2.1122419522912494
2208	7	-25.241040487864296
2208	8	-5.697108241538196
2208	9	3.38178150173707
2208	10	3.197708838131028
2208	11	-21.234220415899603
2208	12	-6.819335433197459
2208	13	1.9486791173911726
2208	14	0.7921876165912174
2208	15	2.5438950555288606
2208	16	2.3633135737892097
2208	17	1.470922212642094
2208	18	0.9343941627352663
2208	19	0.960994800386274
2208	20	0.888772231238093
2208	21	0.8252214206082087
2208	22	0.9599418347868038
2208	23	1.8937000847249337
2208	24	1.4234778746194792
2208	25	1.198684263594025
2208	26	-22.282754470485134
2208	27	-13.084773099662122
2208	28	-3.7908970159437345
2208	29	-1.3205335018133937
2208	30	-0.36256326714569553
2208	31	0.3332554237357641
2208	32	0.9586445401047428
2208	33	0.9779645394291805
2208	34	1.0976490595646595
2208	35	1.2496987565215179
2208	36	1.2428064022241045
2208	37	1.3537508300368515
2208	38	1.2358902940875893
2208	39	1.4283690333385621
2208	40	1.5095929817801306
2208	41	1.47781103260985
2208	42	1.4547849601412821
2208	43	1.7622300180285038
2209	1	-3.062154938056594
2209	2	-3.6554514667350517
2209	3	-3.4824431143601693
2209	4	-2.570216511954289
2209	5	-3.5555421290773808
2209	6	-4.276059668568511
2209	7	-3.0860017281743737
2209	8	-2.9672515349373043
2209	9	-3.213473372587572
2209	10	-0.7378091322706659
2209	11	-2.451274679465495
2209	12	-1.6127345405500542
2209	13	-3.706946910327879
2209	14	-0.13687040549005758
2209	15	1.394494068918572
2209	16	1.2413967309421352
2209	17	0.3398439885406148
2209	18	-0.012164446614036706
2209	19	-0.45126735606192625
2209	20	-0.5022308225556529
2209	21	-0.5642547363098132
2209	22	-1.0195037841936951
2209	23	0.3366176322936454
2209	24	-0.5906165580796028
2209	25	-1.105050761071233
2209	26	-1.5023927133791908
2209	27	-0.9408924325256436
2209	28	-0.9333682398103065
2209	29	-0.8325137696076155
2209	30	-0.5908930768708558
2209	31	-0.723474667878051
2209	32	-1.0179821923359498
2209	33	-1.0533955733262772
2209	34	-1.3610072711846968
2209	35	-2.284597696379592
2209	36	-1.019115138767205
2209	37	-1.4114594119175763
2209	38	-1.4321858158489889
2209	39	-1.5039776897058417
2209	40	-1.7310694565567317
2209	41	-2.166421995344109
2209	42	-2.0325300504295427
2209	43	-1.9091347928711926
2215	1	-4.466547702914475
2215	2	-1.8031074283445
2215	3	1.2222449624209304
2215	4	1.085375957591669
2215	5	3.672770901561229
2215	6	1.6584179984672964
2215	7	2.2589595121357036
2215	8	2.6730216285916732
2215	9	-20.911147791192224
2215	10	-10.487716847294658
2215	11	-3.5834267651059513
2215	12	-21.814284928146954
2215	13	-11.581623912911859
2215	14	0.28930161370521645
2215	15	-27.46837045673665
2215	16	-13.71965900918337
2215	17	-4.154618912899032
2215	18	-2.1492999209588177
2215	19	-1.145065805674328
2215	20	-0.1155567730909084
2215	21	0.13618823157501936
2215	22	0.41737329221826514
2215	23	0.8244360154608685
2215	24	0.9739829251245311
2215	25	0.40214746705722604
2215	26	9.844229656498992
2215	27	6.4981994833104615
2215	28	2.827429102382382
2215	29	0.9723957911159005
2215	30	0.6937137891313583
2215	31	0.07423666471699875
2215	32	0.3266099080701039
2215	33	0.191528752993392
2215	34	0.04209350400910239
2215	35	-0.10888710206434382
2215	36	-0.20885304943534777
2215	37	-0.019986543700518666
2215	38	-0.17969412149682995
2215	39	-0.19500759003806678
2215	40	0.19141116359831045
2215	41	0.17983123463005057
2215	42	0.2506002559565772
2215	43	0.035678791477280924
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	35.0
25	479.0
26	3195.0
27	12789.0
28	32796.0
29	64994.0
30	110224.0
31	157924.0
32	201668.0
33	218064.0
34	177032.0
35	120597.0
36	51875.0
37	29274.0
38	861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	95.3080170636054	2.0285280563746535	0.6606086855548426	2.0028461944650924
2	0.6162586340093873	2.1031317243310887	0.6636436175388747	96.61696602412066
3	3.0525237157611764	89.16906200578943	1.8680683596080248	5.910345918841369
4	1.1648808654333611	1.2740911317139396	96.98876964452961	0.572258358323094
5	0.47477672106561386	1.1274415869757528	98.06615548981365	0.3316262021449828
6	0.5238601324754358	2.0248704522915815	0.6401230660921777	96.81114634914081
7	95.66348368985726	2.1338884472011785	1.1587380426472558	1.0438898202943165
8	2.8753377237777	88.72948166751141	1.5637044564730849	6.831476152237798
9	31.491459058727084	21.232485533141602	24.77021477736865	22.505840630762663
10	28.812665668101783	21.61723431435128	26.446287296779758	23.123812720767184
11	26.71519822518327	24.99491164927016	24.60946908148485	23.680421044061724
12	25.05033084282561	23.725597521807195	28.570297609572265	22.653774025794927
13	30.335183880416157	20.361710581759482	26.596643956057157	22.706461581767204
14	31.62004250640516	27.528949130277645	20.712942954914602	20.13806540840259
15	29.463626437587358	26.86711106012907	23.102087289752184	20.567175212531385
16	21.360866822202368	26.04743133211671	32.26558497372689	20.326116871954035
17	22.06625605322011	28.20091909944839	29.446467237937775	20.286357609393736
18	21.247980555788285	29.53775144143252	27.108832915692382	22.105435087086814
19	22.614737237573923	28.41254382053276	25.865262491654743	23.107456450238576
20	23.006424896070346	26.992432787362425	28.104372195507054	21.89677012106017
21	22.443474368531632	26.991502010900238	28.053094874044792	22.51192874652334
22	22.45760524754846	25.957917057663295	28.875393570365432	22.709084124422812
23	22.54869354618638	26.925399099467867	29.63049188156638	20.89541547277937
24	22.711707221725337	27.746446337775392	29.103518419643105	20.43832802085616
25	21.069986774512632	27.245180904867027	30.94027884370486	20.744553476915474
26	17.60322085490543	29.20129042329006	31.20365793376174	21.991830788042773
27	20.76275883427583	28.167633985423794	30.597847839165876	20.4717593411345
28	21.580923385708108	28.11136881527632	30.49763793119496	19.81006986782061
29	21.522497381073666	28.118553168012372	30.90586927199771	19.453080178916245
30	21.266839678560036	28.053819278443942	31.000577928545013	19.678763114451005
31	21.128117995378272	27.972201937876594	31.082518410335343	19.817161656409795
32	20.67440677808343	28.047848679439742	31.33222035032734	19.945524192149495
33	20.45584354155367	28.447405629843743	31.109510927738746	19.987239900863845
34	19.917770130367938	28.819885446802317	31.592922375781534	19.66942204704821
35	20.167302838275898	28.817346965541805	32.42706731798455	18.588282878197745
36	20.750645831940695	28.11875692264994	31.815547182328107	19.31505006308126
37	20.01414780222523	29.055710355903535	31.065764434015986	19.864377407855248
38	20.66636825409182	29.128395535996088	31.24007348057089	18.965162729341206
39	20.555352006965595	29.54682186376986	31.195311594343927	18.702514534920617
40	20.161041251166644	29.947648054804116	31.770785296101145	18.12052539792809
41	19.515167002451328	29.961017389442794	31.50737555730241	19.01644005080347
42	19.903385403225055	29.571783596164863	32.58690702135455	17.937923979255533
43	19.994178416309236	29.648107266064144	31.872578394647526	18.485135922979094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	25.5
7	49.0
8	201.0
9	353.0
10	800.0
11	1247.0
12	1247.0
13	2298.5
14	3350.0
15	5014.0
16	6678.0
17	8803.0
18	10928.0
19	10928.0
20	12275.5
21	13623.0
22	15747.0
23	17871.0
24	20482.5
25	23094.0
26	23094.0
27	26515.0
28	29936.0
29	35435.5
30	40935.0
31	46996.5
32	53058.0
33	53058.0
34	57689.0
35	62320.0
36	66003.5
37	69687.0
38	73916.5
39	78146.0
40	78146.0
41	82939.0
42	87732.0
43	91665.5
44	95599.0
45	98983.5
46	102368.0
47	102368.0
48	103265.0
49	104162.0
50	100228.5
51	96295.0
52	92000.5
53	87706.0
54	87706.0
55	77176.0
56	66646.0
57	58213.5
58	49781.0
59	42115.5
60	34450.0
61	34450.0
62	27623.0
63	20796.0
64	16472.5
65	12149.0
66	9580.5
67	7012.0
68	7012.0
69	5369.5
70	3727.0
71	2567.0
72	1407.0
73	933.5
74	460.0
75	460.0
76	312.0
77	164.0
78	117.5
79	71.0
80	39.0
81	7.0
82	7.0
83	3.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.16821669152968036
2	0.0
3	0.0
4	1.5230041402629357
5	3.296302532811986
6	4.230802100847091E-4
7	0.16847053965573117
8	0.0
9	0.28989455995004265
10	0.17904754490784888
11	0.22380943113481114
12	0.5992508095639819
13	1.4019185841366921
14	0.02876945428576022
15	0.4166493908914215
16	0.07987754366399309
17	0.0
18	0.014553959226913993
19	0.0
20	0.0
21	0.0
22	0.0
23	0.7758444892533396
24	0.0
25	0.0
26	34.95226385989614
27	0.0016923208403388365
28	5.076962521016509E-4
29	0.0022846331344574295
30	1.6923208403388365E-4
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1181809.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.72146754095098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5692035323513	46.49394406926138
2	11.309924187735753	13.73510388912426
3	5.5677651117650555	10.142486055290435
4	2.8594458419328768	6.945189915041378
5	1.4071387393730326	4.272176464422714
6	0.7175053029272916	2.6140784977295843
7	0.3969861435473698	1.6873906860733245
8	0.2407449384887791	1.169470877447571
9	0.17182348997008598	0.9390037022092342
>10	0.6892891256798317	7.45330239030303
>50	0.0504276985836242	2.084438588221131
>100	0.01848215200290812	1.8069393877396347
>500	9.478017316050425E-4	0.38166509694991957
>1k	3.159339105350142E-4	0.2748103801863582
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAAGTCGAGTCATGTCCGACGATCGTCGGACTGTAGAACTCTG	1764	0.1492626981178854	Illumina DpnII expression Sequencing Primer (100% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	5.923122941185928E-4	0.0	0.0
12	0.0	0.0	7.615443781524764E-4	0.0	0.0
13	0.0	0.0	0.008546220243711124	0.0	0.0
14	0.0	0.0	0.028430990117692453	0.0	0.0
15	0.0	0.0	0.04890807228579237	0.0	0.0
16	0.0	0.0	0.06320818338665554	0.0	0.0
17	0.0	0.0	0.11685475402539666	0.0	0.0
18	0.0	0.0	0.1616166402523589	0.0	0.0
19	0.0	0.0	0.20257080458855872	0.0	0.0
20	0.0	0.0	0.2286325455297768	0.0	0.0
21	0.0	0.0	0.4533727531267743	0.0	0.0
22	0.0	0.0	0.533842609084886	0.0	0.0
23	0.0	0.0	0.6517973716565029	0.0	0.0
24	0.0	0.0	0.7035823893708713	0.0	0.0
25	0.0	0.0	0.7390365109759699	0.0	0.0
26	0.0	0.0	0.7744060165390516	0.0	0.0
27	0.0	0.0	0.8447219474551302	0.0	0.0
28	0.0	0.0	0.9193532965140729	0.0	0.0
29	0.0	0.0	0.9906846199343549	0.0	0.0
30	0.0	0.0	1.073439109026924	0.0	0.0
31	0.0	0.0	1.1671090675396785	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCCA	65	2.7703209E-9	36.785328	20
ATTCGAC	19265	0.0	35.39584	3
ATTCGAG	25015	0.0	35.368122	3
TTCGACC	3970	0.0	35.281616	4
TTCGAGT	7535	0.0	35.27202	4
TTCGAGC	7095	0.0	35.260345	4
TTCGCGT	715	0.0	35.212284	4
TTCGACG	5950	0.0	35.07288	4
TTCGACT	5780	0.0	35.061485	4
TCGAGCG	1580	0.0	35.011395	5
ACTATCG	20	0.0024482314	34.915684	11
CTCGCGA	20	0.0024482314	34.915684	10
ACGCGCC	50	3.3469405E-10	34.914173	8
TCGAGGG	1910	0.0	34.903294	5
GCGTTCG	2885	0.0	34.901142	1
CTTCGCG	305	0.0	34.878944	3
TCGATAC	1495	0.0	34.86728	5
TTCGAGA	4755	0.0	34.863663	4
TTCGATA	4855	0.0	34.802914	4
TTCGATG	6100	0.0	34.791744	4
>>END_MODULE