FastQCFastQC Report
Thu 26 May 2016
SRR2099039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763486
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCATGAGCAAGCTTAGCCATGTTTTTTTTTTTTTTTTTTT10290.13477653814215323No Hit
TAGCCATGCTGGTGTAGTCACCTCCCAGTTGGCCCAGTGAGAG8840.11578470332134447No Hit
TAGCCATGTGGCGGATCGTGGGTAGCCATGTTTTTTTTTTTTT8190.10727112219477501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG505.58608E-737.03081523
CCACGCG200.00225509435.5037774
GCCATGC125550.035.263413
GCCATGG168800.035.219833
CATGCCG9250.035.122345
CATGCGT8750.035.1004075
GCCATGA111550.035.0900153
CCATGTC38450.035.0420884
CCATGCG28450.035.00464
TCCGCAC301.2342096E-534.9240469
CGCGGGC200.002444806534.92404611
CCTAGCG200.002444806534.9240469
CCGTCGG301.2342096E-534.9240469
GTTACGC1000.034.9240468
AACTCGA200.002444806534.9240469
GCGTACT251.7318864E-434.9240468
GCCCGCA251.7318864E-434.9240468
TGCCGTC650.034.9240467
CCATGGC47500.034.79374
CCGTTTA200.00249089434.79133212
Produced by FastQC (version 0.11.5)