##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099039_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 763486 Sequences flagged as poor quality 0 Sequence length 43 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.679297328307264 30.0 26.0 31.0 17.0 35.0 2 25.773128780357467 28.0 23.0 31.0 13.0 35.0 3 27.43994388895147 29.0 25.0 31.0 17.0 35.0 4 28.797178992149167 30.0 27.0 32.0 19.0 37.0 5 28.950009561406496 30.0 27.0 32.0 19.0 39.0 6 24.982028485132666 27.0 19.0 31.0 10.0 34.0 7 27.42542757823981 29.0 25.0 31.0 17.0 35.0 8 27.732564840743642 30.0 25.0 31.0 16.0 37.0 9 25.680167023363886 26.0 26.0 28.0 20.0 31.0 10 25.755489950044925 27.0 26.0 27.0 20.0 31.0 11 25.39057821623448 27.0 26.0 27.0 16.0 31.0 12 27.75603743880045 30.0 26.0 31.0 17.0 35.0 13 27.441239263064418 30.0 25.0 31.0 17.0 35.0 14 30.954857063521793 31.0 31.0 33.0 30.0 34.0 15 30.901176969846205 31.0 31.0 34.0 28.0 34.0 16 31.115563350212053 31.0 31.0 34.0 28.0 34.0 17 35.03452846548594 35.0 35.0 37.0 32.0 37.0 18 35.075054421430124 35.0 35.0 37.0 33.0 37.0 19 34.708709786426994 35.0 35.0 37.0 32.0 37.0 20 34.61210683627467 35.0 35.0 37.0 32.0 37.0 21 34.57540806249231 35.0 35.0 37.0 32.0 37.0 22 36.02917407784818 37.0 35.0 39.0 32.0 39.0 23 35.93958762832586 37.0 35.0 39.0 32.0 39.0 24 36.23595324603202 37.0 35.0 39.0 32.0 39.0 25 36.42336467204376 37.0 35.0 39.0 32.0 39.0 26 22.72321168953982 32.0 2.0 37.0 2.0 39.0 27 29.572764922997933 34.0 17.0 38.0 17.0 40.0 28 34.264124031088976 36.0 32.0 39.0 27.0 40.0 29 36.37615097067923 37.0 34.0 39.0 32.0 40.0 30 37.19244491713011 38.0 36.0 40.0 33.0 40.0 31 37.58261710103394 39.0 37.0 40.0 33.0 41.0 32 37.91026554514425 39.0 37.0 40.0 34.0 41.0 33 38.00470473590871 39.0 37.0 40.0 34.0 41.0 34 38.03461360129721 39.0 37.0 40.0 34.0 41.0 35 38.05152026363286 39.0 37.0 40.0 34.0 41.0 36 38.01900362285622 39.0 37.0 40.0 34.0 41.0 37 38.010389188537836 39.0 37.0 40.0 34.0 41.0 38 37.938691737635004 39.0 37.0 40.0 33.0 41.0 39 37.781210133519146 39.0 37.0 40.0 33.0 41.0 40 37.65445862792507 39.0 37.0 40.0 33.0 41.0 41 37.56071624103127 39.0 37.0 40.0 33.0 41.0 42 37.467260696332346 39.0 36.0 40.0 33.0 41.0 43 37.40229159408293 39.0 36.0 40.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.996474786473872 1101 2 5.363033864597142 1101 3 5.207886143173589 1101 4 7.945957967628363 1101 5 8.902588915871853 1101 6 5.877709131006753 1101 7 6.449590457651965 1101 8 6.063087491542454 1101 9 5.891809336567217 1101 10 5.548227180933889 1101 11 3.3920635429712256 1101 12 7.835912615607253 1101 13 6.7263015861477555 1101 14 0.08392684190205557 1101 15 1.0673682742550916 1101 16 1.5595215741157098 1101 17 0.833283159437471 1101 18 0.28752897764677954 1101 19 0.1221930432282079 1101 20 -0.029748811181541157 1101 21 0.007068232848467915 1101 22 -0.02522672392346692 1101 23 0.921268595457228 1101 24 0.35216835021164883 1101 25 0.28360073838841515 1101 26 12.017978466154414 1101 27 7.022579611605835 1101 28 2.8695704504417563 1101 29 1.2421789215161283 1101 30 0.5276500871442309 1101 31 0.23203642994025842 1101 32 0.05649876860672265 1101 33 -0.05388667643008205 1101 34 0.07991432012524768 1101 35 0.10325845138049061 1101 36 -0.05922379741777917 1101 37 -0.10614025549394057 1101 38 -0.009989380719694907 1101 39 -0.15645825918500122 1101 40 -0.1751574369050246 1101 41 -0.25977580531549194 1101 42 -0.21254195245607832 1101 43 -0.2011597577336417 1102 1 3.3940491700968245 1102 2 2.4789205329502906 1102 3 1.239695155478426 1102 4 4.051792549607647 1102 5 5.090023645742711 1102 6 2.617296072440393 1102 7 3.1043979067169403 1102 8 1.8578590704147189 1102 9 4.40512314561844 1102 10 3.546093131493997 1102 11 3.8929842977371614 1102 12 5.564650283201484 1102 13 4.029525687284831 1102 14 -0.14319828863820305 1102 15 1.1851716952206708 1102 16 1.6895558875550307 1102 17 0.8926872472124145 1102 18 0.3132540031061879 1102 19 0.19485126450773294 1102 20 0.1370709362352187 1102 21 -0.0159827443386078 1102 22 -0.0857153498940022 1102 23 0.8278238245270089 1102 24 0.38544103502746196 1102 25 0.31863709506656335 1102 26 12.004848872921098 1102 27 7.09372583615454 1102 28 2.9592024728590047 1102 29 1.384768451052281 1102 30 0.6301240277411111 1102 31 0.36926197107422354 1102 32 0.25012937697297133 1102 33 0.0600514555955769 1102 34 0.19667893252608337 1102 35 0.13268790126276286 1102 36 0.08411946021841743 1102 37 0.07103028035354697 1102 38 -0.04903243143742486 1102 39 -0.07347065483387638 1102 40 -0.058243635637566626 1102 41 -0.19329659770296814 1102 42 -0.14390047924845106 1102 43 -0.04795511601394509 1103 1 0.4027565624782703 1103 2 -0.805831062884824 1103 3 0.29200599051225495 1103 4 1.9977720398519203 1103 5 2.179097441153221 1103 6 -1.6765739867324214 1103 7 -0.2487705002062519 1103 8 -1.0237028565480095 1103 9 2.221243448223735 1103 10 1.403121880106422 1103 11 -1.2755871925451565 1103 12 1.7803185761859908 1103 13 1.3533866868461182 1103 14 -0.6205978075989869 1103 15 1.3360614869883136 1103 16 1.6958105268410115 1103 17 0.885916118918253 1103 18 0.36214132745731575 1103 19 0.14964643842175462 1103 20 0.09049626993685678 1103 21 0.05883930325249054 1103 22 0.01897943908561217 1103 23 0.8981159672757286 1103 24 0.525949526530809 1103 25 0.3128666456487963 1103 26 12.053260825267383 1103 27 7.155146712971167 1103 28 2.902244227522665 1103 29 1.2098226363272815 1103 30 0.5770074975961066 1103 31 0.1736096334685513 1103 32 0.11884536132195933 1103 33 0.06249955206656921 1103 34 0.09678205198891021 1103 35 0.03790270639591142 1103 36 -0.0017796071488689336 1103 37 -0.054630316320547934 1103 38 -0.10788443677807891 1103 39 -0.0039185190681081394 1103 40 -0.053167320497827575 1103 41 -0.011837552417986785 1103 42 0.13421116639471364 1103 43 0.1499724284944648 1104 1 1.192343009275639 1104 2 -1.0368152573548386 1104 3 -0.6299141654955918 1104 4 1.089815865739883 1104 5 0.67382806881173 1104 6 -1.0797190636587644 1104 7 0.15676183126437948 1104 8 -0.5534088540287634 1104 9 2.1300725625922112 1104 10 1.08662417512517 1104 11 0.6942548176228485 1104 12 2.6507416982583507 1104 13 1.3083946258824284 1104 14 0.21527231806660652 1104 15 1.836584226886643 1104 16 1.8149716763429318 1104 17 0.7662475933509825 1104 18 0.18496180407826301 1104 19 0.006983481742381059 1104 20 -0.0190574682632203 1104 21 -0.043612965119777414 1104 22 -0.11632154896196312 1104 23 0.7380802640509216 1104 24 0.4020846154874036 1104 25 0.06793335794587563 1104 26 12.030872922379903 1104 27 6.92832680359772 1104 28 2.77122959048873 1104 29 1.044979584480842 1104 30 0.5054731744236349 1104 31 0.10933844354866551 1104 32 -0.002518571135041725 1104 33 -0.13283020172246296 1104 34 -0.14440098215797548 1104 35 -0.17601422310141146 1104 36 -0.2314283624724922 1104 37 -0.23853634752781971 1104 38 -0.3086611181331307 1104 39 -0.3646318570820384 1104 40 -0.4154843047769887 1104 41 -0.3782956968492073 1104 42 -0.27420449719669193 1104 43 -0.18671504252675675 1105 1 1.114565868020744 1105 2 -2.565839208940112 1105 3 -1.03339419771061 1105 4 -0.36495152530225283 1105 5 -2.043629245056234 1105 6 -2.3910686371275176 1105 7 -4.235281152827497 1105 8 -2.2619774988071484 1105 9 1.6894407534601612 1105 10 0.6923342403943771 1105 11 1.0952690447297861 1105 12 3.0632036029232843 1105 13 0.4998595029849042 1105 14 0.18342138436586097 1105 15 1.7832232331530875 1105 16 1.588533720801717 1105 17 0.6817969572296576 1105 18 0.22454268658093923 1105 19 -0.005459782250490264 1105 20 -0.14836622156925472 1105 21 -0.08446132381195781 1105 22 -0.2463858872527851 1105 23 0.6921783363637459 1105 24 0.3417124465254844 1105 25 0.15871656379685106 1105 26 11.94511777488027 1105 27 6.665658810974321 1105 28 2.800728621867492 1105 29 1.1346326016850838 1105 30 0.38989720339119316 1105 31 0.017996241313255723 1105 32 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0.4486560534437274 1106 26 12.036185557242863 1106 27 7.036624010395997 1106 28 2.8493350047825814 1106 29 1.2724680924832654 1106 30 0.6200800363538406 1106 31 0.21166279854967485 1106 32 -0.0915221997532285 1106 33 0.0030364694761146893 1106 34 0.12110304351735834 1106 35 -0.07489597423155914 1106 36 0.026625820635224784 1106 37 -0.08364492282911584 1106 38 -0.2185801308358748 1106 39 -0.2660216085788605 1106 40 -0.24137449041020886 1106 41 -0.19948402553218614 1106 42 -0.07615589657849853 1106 43 -0.22462035238067557 1107 1 1.8925393325616362 1107 2 0.47074224630134154 1107 3 -3.4215291497603957 1107 4 -24.31703638265412 1107 5 -24.312100349664867 1107 6 -8.172590768381838 1107 7 -9.022153215438351 1107 8 -1.452040981485947 1107 9 1.2029327016318412 1107 10 1.7905845836117003 1107 11 2.5172647657867984 1107 12 4.126933047462373 1107 13 2.52116653468115 1107 14 0.157113901727147 1107 15 1.8713849912274618 1107 16 1.7642948114812391 1107 17 1.0315805048441717 1107 18 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2215 38 0.11547470321896469 2215 39 0.08409864154139512 2215 40 0.07613392709820488 2215 41 0.3471249210767624 2215 42 0.2705492501266491 2215 43 0.8173228895625684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 3.0 24 25.0 25 363.0 26 2477.0 27 9607.0 28 23982.0 29 45384.0 30 73292.0 31 100230.0 32 125224.0 33 135689.0 34 111251.0 35 79180.0 36 34999.0 37 20746.0 38 1034.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.1822174301793509 1.7059380423920474 96.42704991321727 0.684794614211329 2 2.1019376910644074 93.43786264581145 1.0182243027377058 3.441975360386438 3 96.34387532973754 1.8926345735219767 0.7178913562265713 1.045598740513906 4 0.843590888314891 2.359769125290985 0.7436727361629304 96.05296725023119 5 0.43835749798196 2.1958438517048213 0.7905849138496361 96.57521373646358 6 2.4288846567943017 90.48119264733234 0.7827337526425122 6.307188943230846 7 1.3087098301162363 1.4864829327302371 94.8002682375217 2.404538999631831 8 92.89233856285512 3.401896040005973 1.3501229885027362 2.355642408636177 9 29.635061991664415 21.681456600582493 26.00196440436266 22.681517003390436 10 26.101483687310605 20.164110771491163 28.804392029280194 24.930013511918037 11 26.654469791030955 22.14693070140365 27.365369641617036 23.833229865948358 12 26.75875906978949 23.315958484688498 27.080801604156267 22.844480841365744 13 27.54983099036068 20.57090647249234 30.262074343023926 21.617188194123052 14 34.43705225425408 26.310024788147807 19.408910107589993 19.844012850008124 15 30.919181244117755 26.858920769121568 22.980977017839958 19.240920968920715 16 21.87874839249266 26.356434683093283 32.54656691490994 19.21825000950412 17 23.700893009171093 29.358757069546787 27.794746727510393 19.145603193771727 18 22.53757113363167 31.8345926911831 25.978971693268637 19.6488644819166 19 23.49172086979984 30.929447298313264 23.203568893208256 22.375262938678638 20 23.081628215841548 29.744487783665974 25.31310331820099 21.860780682291487 21 21.86798448170628 28.758615089209233 26.912739722797795 22.46066070628669 22 21.736089463330043 28.607859214183364 27.23154059144503 22.424510731041565 23 22.73546841757468 28.213079314497353 28.964636705493174 20.086815562434793 24 22.941612550852277 29.66812750986921 27.05747060194948 20.33278933732904 25 21.881999146022324 29.016380130087523 28.56974456637057 20.531876157519587 26 18.550213085986464 30.78706442717473 30.07651040360993 20.58621208322888 27 20.20872927409732 30.92431075199384 28.956995430117736 19.909964543791105 28 20.386965963655797 30.472390822001422 30.31835887871032 18.82228433563246 29 21.281021233344116 30.325003372763003 29.62857692977711 18.765398464115766 30 21.82701690275513 30.129996005160546 29.445634164390917 18.597352927693407 31 22.03419578093115 30.327340645408036 28.812447117563387 18.826016456097427 32 21.929544222159937 30.315683588173194 29.5550933481426 18.19967884152427 33 21.25382783705268 31.641575615008005 29.127711575588812 17.976884972350508 34 20.545890821835634 31.353816572929954 28.96058342916569 19.139709176068717 35 21.10542956910801 31.183152015885035 28.61925431507585 19.092164099931104 36 20.8674422320776 31.46449312757536 29.12443712130936 18.543627519037678 37 20.832471060373077 31.77975758560078 29.513966202392712 17.87380515163343 38 21.198293092473207 31.61289139552002 29.794390466884792 17.39442504512198 39 21.31237507956924 31.747405977319822 29.420185831829265 17.520033111281673 40 21.36502830438279 31.769934222762437 29.002365465771472 17.8626720070833 41 22.22201847840039 30.944247831656373 29.042706742494296 17.791026947448938 42 21.589262933439514 31.4499545505746 29.378010860709953 17.58277165527593 43 22.460398749944336 31.001092357947623 28.605108672588624 17.933400219519417 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.0 7 4.0 8 38.5 9 73.0 10 169.5 11 266.0 12 266.0 13 570.5 14 875.0 15 1548.5 16 2222.0 17 3313.5 18 4405.0 19 4405.0 20 6058.0 21 7711.0 22 10012.0 23 12313.0 24 14195.0 25 16077.0 26 16077.0 27 19610.5 28 23144.0 29 25137.0 30 27130.0 31 31224.0 32 35318.0 33 35318.0 34 39005.0 35 42692.0 36 46396.0 37 50100.0 38 54906.0 39 59712.0 40 59712.0 41 61576.5 42 63441.0 43 63953.5 44 64466.0 45 64484.0 46 64502.0 47 64502.0 48 63738.5 49 62975.0 50 60387.0 51 57799.0 52 54284.0 53 50769.0 54 50769.0 55 44834.5 56 38900.0 57 33694.0 58 28488.0 59 24108.0 60 19728.0 61 19728.0 62 16872.0 63 14016.0 64 11309.0 65 8602.0 66 6386.0 67 4170.0 68 4170.0 69 3055.0 70 1940.0 71 1393.0 72 846.0 73 662.0 74 478.0 75 478.0 76 342.0 77 206.0 78 150.5 79 95.0 80 55.5 81 16.0 82 16.0 83 11.0 84 6.0 85 3.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.3884812557139227 2 0.0 3 0.0 4 1.4237327207047674 5 3.1312951383522423 6 0.0010478253694239318 7 0.3884812557139227 8 0.0 9 0.25304982671587956 10 0.1566498927288778 11 0.4167725406883689 12 0.5185425796936682 13 1.229492092847806 14 0.02855324131680214 15 0.3565225819464928 16 0.08631461480629639 17 0.0 18 0.01755107493785086 19 0.0 20 0.0 21 0.0 22 0.0 23 0.6977207178651605 24 0.0 25 0.0 26 34.691009396374 27 0.0011788035406019232 28 0.0010478253694239318 29 0.002226628910025855 30 1.3097817117799148E-4 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 763486.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.68037655244196 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58573032216525 52.12315381991187 2 10.581303826909464 13.688054318805984 3 3.92278234911658 7.611811184223995 4 1.9061736696024865 4.931681228969556 5 0.983060037424896 3.179234669714997 6 0.565750878969201 2.195578793196175 7 0.3466433766225669 1.5694716890550304 8 0.22661581032735947 1.17260767557683 9 0.1421350132751248 0.8274011561929269 >10 0.6806328372662405 7.786632644129055 >50 0.03663029445553082 1.6142851723841452 >100 0.02089792670860705 2.5519523561785764 >500 0.001408848991437618 0.5918561168497772 >1k 2.34808165239603E-4 0.15627917481098813 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAGCCATGAGCAAGCTTAGCCATGTTTTTTTTTTTTTTTTTTT 1029 0.13477653814215323 No Hit TAGCCATGCTGGTGTAGTCACCTCCCAGTTGGCCCAGTGAGAG 884 0.11578470332134447 No Hit TAGCCATGTGGCGGATCGTGGGTAGCCATGTTTTTTTTTTTTT 819 0.10727112219477501 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0015717380541358976 0.0 0.0 14 0.0 0.0 0.004846192333585684 3.929345135339744E-4 0.0 15 0.0 0.0 0.008120646613035472 5.239126847119659E-4 0.0 16 0.0 0.0 0.010347275523061326 5.239126847119659E-4 0.0 17 0.0 0.0 0.013752707973689104 5.239126847119659E-4 0.0 18 0.0 0.0 0.0168961840819609 5.239126847119659E-4 0.0 19 0.0 0.0 0.021873354586724576 5.239126847119659E-4 0.0 20 0.0 0.0 0.029601066686226075 5.239126847119659E-4 0.0 21 0.0 0.0 0.03732877878572757 5.239126847119659E-4 0.0 22 0.0 0.0 0.042305949290491245 5.239126847119659E-4 0.0 23 0.0 0.0 0.06339343485014788 5.239126847119659E-4 0.0 24 0.0 0.0 0.08814830920278827 5.239126847119659E-4 0.0 25 0.0 0.0 0.1351694726556872 5.239126847119659E-4 0.0 26 0.0 0.0 0.14499283549403658 5.239126847119659E-4 0.0 27 0.0 0.0 0.17734444377500047 5.239126847119659E-4 0.0 28 0.0 0.0 0.2030161653258868 5.239126847119659E-4 0.0 29 0.0 0.0 0.2210911529484496 5.239126847119659E-4 0.0 30 0.0 0.0 0.25422863025648146 5.239126847119659E-4 0.0 31 0.0 0.0 0.28134111169032566 5.239126847119659E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTCG 50 5.58608E-7 37.030815 23 CCACGCG 20 0.002255094 35.503777 4 GCCATGC 12555 0.0 35.26341 3 GCCATGG 16880 0.0 35.21983 3 CATGCCG 925 0.0 35.12234 5 CATGCGT 875 0.0 35.100407 5 GCCATGA 11155 0.0 35.090015 3 CCATGTC 3845 0.0 35.042088 4 CCATGCG 2845 0.0 35.0046 4 TCCGCAC 30 1.2342096E-5 34.924046 9 CGCGGGC 20 0.0024448065 34.924046 11 CCTAGCG 20 0.0024448065 34.924046 9 CCGTCGG 30 1.2342096E-5 34.924046 9 GTTACGC 100 0.0 34.924046 8 AACTCGA 20 0.0024448065 34.924046 9 GCGTACT 25 1.7318864E-4 34.924046 8 GCCCGCA 25 1.7318864E-4 34.924046 8 TGCCGTC 65 0.0 34.924046 7 CCATGGC 4750 0.0 34.7937 4 CCGTTTA 20 0.002490894 34.791332 12 >>END_MODULE