FastQCFastQC Report
Thu 26 May 2016
SRR2099038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177626
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAAGCTGGGAGCGATCTTAGCCGTAAGCTTACGCTCCCGAT7010.39464943195252944No Hit
CGTTAGCTGCTCAACAGTCCGACGATCAGCGTCGTAAGCGATC4670.26291196108677783No Hit
CGTAAGCTGGGAGCGATCTTAGCCGNAAGCTTACGCTCCCGAT4540.2555932127053472No Hit
CGTAAGCCTGTTTGCCAGCCTTGCTGCCTTTTGTTGCTCTGCT3550.1998581288775292No Hit
CGTTAGCTGCTCAACAGTCCGACGANCAGCGTCGTAAGCGATC3250.18296870953576616No Hit
CGTAAGCTGTTTAGAAAATGGAGCTGTGCGAACCAATTTAGAG2600.1463749676286129No Hit
CGTAAGCCTGTTTGCCAGCCTTGCTNCCTTTTGTTGCTCTGCT2570.14468602569443662No Hit
CGAAAGCCCATGCCAGTCCGACGATCCATGGATTGGACGTCAG2350.13230045151047706No Hit
CGTAAGCTGGGAGGTCCGTCGATCTTAGCCGTAAGCTTACGCT2300.1294855482868499No Hit
CGTAAGCTTGTCATAGGCGACACCCGTTGTTAACTTATCCATG2270.1277966063526736No Hit
CGTAAGCTTGTCATAGGCGACACCCNTTGTTAACTTATCCATG2030.11428507087926318No Hit
CGTATGCTCAGTATTTATTTCTCCTTCACTTATGAAGCTTAGT1980.111470167655636No Hit
CGTAAGCTGGGAGCAGTCCGACGATCTTAGCCGTAAGCTTACG1910.10752930314255796No Hit
AGTAAGCCTCGATGATTCTGTAGTGCCAATACAGAAACCGCCT1820.10246247734002904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCCG458.731149E-1152.24495320
TCTCGCG150.00707129352.24495321
CCCGTTG502.4556357E-1047.02045423
ACCCGTT502.4556357E-1047.02045422
GCAAGGG551.4551915E-1146.8511324
CCGTTGT502.8194336E-1046.3826224
ATCAGCG900.042.94687325
CCATGGA557.166818E-1042.1660226
GATCCTG900.040.63496423
GGCAAGG657.2759576E-1140.1884223
GTTGTTA601.6807462E-938.65218426
CCTGCAC406.1123537E-638.65218426
CTGCCTT1200.036.5048424
CACCCGT653.2177923E-936.1695821
TCAGCGT1150.035.85130326
AGCCGTC357.5487696E-735.6104285
AGATAGT200.002217385235.6104285
CGCTGGG650.035.6104285
CGCTATC200.002217385235.6104285
AGATTCG200.002217385235.6104285
Produced by FastQC (version 0.11.5)