FastQCFastQC Report
Thu 26 May 2016
SRR2099036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727048
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACAATCCGACGGACAAGTGCCGCAGCCGTCGCGTAGAGGT8400.11553570053146422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTT251.3312556E-553.94036525
ATCGGCG2450.048.43624524
GCCGCAG1350.046.4181222
GATCGGC2700.045.41401323
CCGCAGC1400.044.76524423
CGCAGCC1500.041.35428224
GCTCGCC609.877112E-1040.86812620
TGCCGCA1550.040.42868821
CGAATAG359.1471506E-538.92202822
ACCAGTT801.2732926E-1137.08424
GTGCCGC1700.036.8614520
GCAGCCG1700.036.48907525
GAGGCGC300.002014303336.32722521
TCGGCGG3300.035.14296325
TCCGCTG301.2558656E-534.8351488
CCCTCCG251.7579446E-434.83514810
CGACGGG251.7579446E-434.83514810
GCGCGAG301.2558656E-534.8351489
CGGGGTA251.7579446E-434.83514811
TAACCCG454.7166395E-934.8351488
Produced by FastQC (version 0.11.5)