FastQCFastQC Report
Thu 26 May 2016
SRR2099035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238419
Sequences flagged as poor quality0
Sequence length43
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTCAGGGTTGGTATCAACCCCTCACGGGTTGGTGAAGAGA5350.22439486785868576No Hit
CATGTCAGCTTGCCCACAATAAAATATCACCTCATACCTGTTA4390.184129620541987No Hit
CATGTCAGTAGGCAGAGAAAAGCACTTGGGAGAAAAATAACGT3870.16231927824544184No Hit
CATGTCAGGGTTGGTATCAACCCCTNACGGGTTGGTGAAGAGA3640.1526723960758161No Hit
CATGTCAGTAGGCAGAGAAAAGCACNTGGGAGAAAAATAACGT2890.12121517160964519No Hit
CATGTCAGGTCAGAATGAGCTCATGGGTTTGAGGAAAGTATCC2760.1157625860355089No Hit
CATGTCAGCTTGCCCACAATAAAATNTCACCTCATACCTGTTA2680.11240714875911735No Hit
CATGTCAGGATACTGTGTCATGGCACATGCCTGTAGCCTCAGC2500.10485741488723632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCACG1050.052.71063623
TCACGGG1050.052.10418725
CTCACGG1050.052.10418724
CCCTCAC1100.050.31469722
CACGGGT1100.049.73581726
GGCACAT454.7220965E-946.853922
CTATAAC304.4694025E-543.9255320
CCACTGT1200.043.9255322
CACTGTT1250.042.1685123
TGCAGGC259.659538E-442.1685121
GCCACTG1250.042.1685121
ATCGGGT250.001022591341.6833524
CACAAAC250.001022591341.6833524
CACATGC553.0770025E-837.89395524
ACATGCC553.0770025E-837.89395525
ATCGGCG351.1871381E-437.21727424
TCCGGCT357.677536E-735.542625
GACAGGT301.0910275E-535.542624
TGCCAGG750.035.542623
TCCGATT357.677536E-735.542625
Produced by FastQC (version 0.11.5)