##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099035_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 238419 Sequences flagged as poor quality 0 Sequence length 43 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.40447279788943 30.0 27.0 31.0 19.0 35.0 2 25.356980777538702 28.0 21.0 31.0 12.0 35.0 3 27.7339599612447 29.0 26.0 31.0 18.0 35.0 4 27.288278199304585 29.0 25.0 31.0 13.0 37.0 5 26.10877069361083 29.0 23.0 31.0 8.0 37.0 6 28.284696270012038 30.0 26.0 31.0 17.0 37.0 7 23.795096028420552 27.0 17.0 30.0 8.0 34.0 8 26.110519715291147 28.0 23.0 31.0 13.0 35.0 9 25.465051023618084 26.0 26.0 27.0 20.0 31.0 10 25.474710488677495 27.0 26.0 27.0 18.0 31.0 11 25.109097848745275 27.0 25.0 27.0 16.0 31.0 12 27.26033998968203 30.0 25.0 31.0 17.0 35.0 13 26.889060855049305 30.0 24.0 31.0 16.0 35.0 14 30.83760522441584 31.0 31.0 31.0 28.0 34.0 15 30.801232284339754 31.0 31.0 33.0 28.0 34.0 16 31.053854768286083 31.0 31.0 34.0 28.0 34.0 17 34.92206996925581 35.0 35.0 37.0 32.0 37.0 18 34.99986997680554 35.0 35.0 37.0 32.0 37.0 19 34.818101745246814 35.0 35.0 37.0 32.0 37.0 20 34.799583925777725 35.0 35.0 37.0 32.0 37.0 21 34.83071818940605 35.0 35.0 37.0 32.0 37.0 22 36.32307827815736 37.0 35.0 39.0 32.0 39.0 23 36.12185270469216 37.0 35.0 39.0 32.0 39.0 24 36.366564745259396 37.0 35.0 39.0 32.0 39.0 25 36.50813064395036 37.0 35.0 39.0 32.0 39.0 26 23.049148767505944 32.0 2.0 37.0 2.0 39.0 27 29.783393940919137 34.0 17.0 38.0 17.0 40.0 28 34.479047391357234 36.0 32.0 39.0 27.0 40.0 29 36.45860858404741 37.0 35.0 39.0 32.0 40.0 30 37.19074402627307 38.0 36.0 40.0 33.0 40.0 31 37.54896631560404 38.0 37.0 40.0 33.0 41.0 32 37.842378333941504 39.0 37.0 40.0 34.0 41.0 33 37.940210302031296 39.0 37.0 40.0 34.0 41.0 34 37.972460248554015 39.0 37.0 40.0 34.0 41.0 35 37.994799072221596 39.0 37.0 40.0 34.0 41.0 36 37.934896128244816 39.0 37.0 40.0 33.0 41.0 37 37.95292740930882 39.0 37.0 40.0 33.0 41.0 38 37.89088956836494 39.0 37.0 40.0 33.0 41.0 39 37.7282892722476 39.0 37.0 40.0 33.0 41.0 40 37.562455173455135 39.0 37.0 40.0 33.0 41.0 41 37.43137082195631 39.0 36.0 40.0 33.0 41.0 42 37.23699453483154 39.0 36.0 40.0 32.0 41.0 43 37.08009848208406 39.0 36.0 40.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.778414664549956 1101 2 5.073678860577424 1101 3 4.74519887552065 1101 4 8.305531287459896 1101 5 8.454989725040171 1101 6 6.354891682915095 1101 7 5.528361105411573 1101 8 5.578759452085528 1101 9 5.895130259984384 1101 10 5.415952438301492 1101 11 3.728519065266628 1101 12 7.992209196274583 1101 13 6.6287018990580755 1101 14 0.1482455630011721 1101 15 0.9635388828858531 1101 16 1.7577230390485283 1101 17 1.0585841766860824 1101 18 0.35492925102531103 1101 19 0.07119851716918646 1101 20 0.047774787262596874 1101 21 0.1453876949926567 1101 22 0.26368869877822476 1101 23 0.9987453108518238 1101 24 0.5403506712599437 1101 25 0.27017255598281054 1101 26 12.008319397323294 1101 27 7.185939093195142 1101 28 3.057286275438571 1101 29 1.3043292818561625 1101 30 0.5724041107848521 1101 31 0.23550449550412367 1101 32 0.04911925943778783 1101 33 -0.005231062075090165 1101 34 -0.036131629010419886 1101 35 0.11173337605743683 1101 36 0.025577737329562922 1101 37 -0.045525694550839546 1101 38 -0.14489773373740178 1101 39 -0.15525591871973887 1101 40 -0.11274511778543683 1101 41 -0.06114926279970234 1101 42 -0.24288159893774264 1101 43 -0.21214243798980448 1102 1 3.0811853058788294 1102 2 2.1400002590495433 1102 3 1.537484034425578 1102 4 4.1872837274457595 1102 5 4.6784608258204194 1102 6 3.2538820453107675 1102 7 3.503424313959002 1102 8 1.7331820114200056 1102 9 4.297165507245346 1102 10 3.297626717180325 1102 11 3.767993822627364 1102 12 5.430779663610036 1102 13 3.826281283556348 1102 14 -0.16775469527031106 1102 15 1.3006443392107876 1102 16 1.7696867858881014 1102 17 1.0650715485698328 1102 18 0.3738289465368254 1102 19 0.13991572348732717 1102 20 0.06544599407008889 1102 21 0.20483261520035967 1102 22 0.15240427390804 1102 23 0.9861086988299661 1102 24 0.7492549205055141 1102 25 0.2965791160785045 1102 26 12.033864486087122 1102 27 6.9657048817387555 1102 28 2.833884839992848 1102 29 1.3518087560697936 1102 30 0.6862134792431078 1102 31 0.4521025163289565 1102 32 0.0886721779055577 1102 33 0.05763255698806802 1102 34 0.2123493268659331 1102 35 0.37728743635703665 1102 36 0.24855098584109925 1102 37 0.1731639854643845 1102 38 0.139680060352255 1102 39 -0.01751056080983915 1102 40 0.14158092957340074 1102 41 0.06630431629933753 1102 42 0.10926647218207108 1102 43 -0.010310069776224395 1103 1 -0.5609539402866552 1103 2 -0.5055393241404964 1103 3 -0.08483352524152465 1103 4 1.8544891748630512 1103 5 1.9116998725223944 1103 6 -0.3053766726203122 1103 7 -1.6249717242829682 1103 8 -1.3163829517824155 1103 9 2.3686241701520103 1103 10 1.7213233263006202 1103 11 -1.0793993956132297 1103 12 2.06992695709652 1103 13 1.315478442920817 1103 14 -0.5862024908545997 1103 15 1.2710859101578933 1103 16 1.666167802464205 1103 17 1.0493466018747384 1103 18 0.47394329415358527 1103 19 0.18556019697243187 1103 20 0.06802526362095307 1103 21 0.2185328352980278 1103 22 0.0041048722985763675 1103 23 0.7600142287838807 1103 24 0.5149798672006085 1103 25 0.3216969027213281 1103 26 11.889141671588188 1103 27 6.849491593342034 1103 28 2.6356335847483336 1103 29 0.8533606165828971 1103 30 0.3970601674610066 1103 31 0.08052434726644009 1103 32 -0.07051174120854853 1103 33 -0.2232541958835199 1103 34 -0.2795087789185047 1103 35 -0.1909514383936326 1103 36 -0.09192119243800789 1103 37 -0.30845871898571886 1103 38 -0.2695210538921913 1103 39 -0.3328305621854497 1103 40 -0.49033151282291243 1103 41 -0.2769878633553873 1103 42 -0.5186867914536677 1103 43 -0.3168132680704687 1104 1 0.8519241973812015 1104 2 -0.7538898890637604 1104 3 -0.8251921975948342 1104 4 1.5627559366005563 1104 5 0.4594938768974117 1104 6 -1.3177039715960994 1104 7 -0.2835683730358234 1104 8 0.08197707621066286 1104 9 1.9271290491347557 1104 10 0.8540868948022435 1104 11 0.49819667655258115 1104 12 2.1868871989989955 1104 13 0.81311377582281 1104 14 0.24715945175958254 1104 15 1.866493460367181 1104 16 1.8889801828003847 1104 17 1.115658513458854 1104 18 0.33631504755038577 1104 19 0.052717402769992816 1104 20 -0.20040241431777162 1104 21 -0.0936846480535678 1104 22 0.0077140431135873655 1104 23 0.834067151552027 1104 24 0.4071206776795293 1104 25 0.2358877662374539 1104 26 11.761225175476856 1104 27 7.005436381831419 1104 28 2.4891133535853456 1104 29 1.0807526825690985 1104 30 0.2956127579213472 1104 31 0.046938151904662107 1104 32 -0.03307759757235118 1104 33 -0.1285915873333039 1104 34 -0.16426509386707977 1104 35 -0.3004930737001317 1104 36 -0.31516161162999623 1104 37 -0.3778230207652129 1104 38 -0.3708386213110586 1104 39 -0.4257425028189914 1104 40 -0.7250618593473277 1104 41 -0.6838756577558911 1104 42 -0.6153509493064746 1104 43 -0.5598546106820166 1105 1 1.1791877610297838 1105 2 -2.666399339051157 1105 3 -1.1049491579188881 1105 4 -1.4850711107411563 1105 5 -2.8198244173448614 1105 6 -2.353242146434088 1105 7 -2.664097198997389 1105 8 -1.1327266375177167 1105 9 1.458179452178662 1105 10 0.7125533057359164 1105 11 1.0643621438875108 1105 12 3.1916627481871522 1105 13 0.2725464965791815 1105 14 0.14832269465303582 1105 15 1.6832305936065595 1105 16 1.4739616250473517 1105 17 0.753394996476878 1105 18 0.050665650638471504 1105 19 -0.009955846999005757 1105 20 -0.16616468219029912 1105 21 -0.1474663541224075 1105 22 -0.05893520135408892 1105 23 0.6931526727713333 1105 24 0.3717349470827287 1105 25 0.2936205781492589 1105 26 11.840359292186921 1105 27 6.702053675230584 1105 28 2.7761503042027442 1105 29 0.9274373314340139 1105 30 0.3812926436814976 1105 31 0.02842084326107397 1105 32 -0.14143745309254285 1105 33 -0.15394088686757357 1105 34 0.007415121670412361 1105 35 -0.018589449571628336 1105 36 -0.3054238468359074 1105 37 -0.13551756606137388 1105 38 -0.132957160708564 1105 39 -0.3181075352211664 1105 40 -0.2423767251497253 1105 41 -0.36284675246109543 1105 42 -0.40616633933039736 1105 43 -0.30157362172333535 1106 1 -3.509589686067912 1106 2 -1.6457508415625064 1106 3 -7.007160548415612 1106 4 0.17128458646003963 1106 5 0.9451995244137841 1106 6 -0.7427718256223343 1106 7 -3.1735809475034706 1106 8 -2.5221250861854934 1106 9 -0.6390889220470406 1106 10 0.46616800355420906 1106 11 1.2089688038364272 1106 12 2.8976579520240833 1106 13 -3.9339181688220464 1106 14 0.12067679834077083 1106 15 1.6086385408643906 1106 16 1.876125366366999 1106 17 0.8748347827585974 1106 18 0.37512686342719803 1106 19 0.0795511793017738 1106 20 0.0961557546198577 1106 21 0.04572840603038486 1106 22 -0.057321378405930545 1106 23 0.8203712887580252 1106 24 0.5304988805613533 1106 25 0.37703788339301525 1106 26 11.983797375596414 1106 27 7.050814406059597 1106 28 2.725576561242711 1106 29 1.2006155315449334 1106 30 0.5631328392774648 1106 31 0.31247242935376107 1106 32 -0.08726736896441167 1106 33 -0.07999092631484217 1106 34 -0.009858876720450382 1106 35 -0.08474681683742347 1106 36 -0.0148201083283368 1106 37 -0.06315486989908692 1106 38 -0.18368244367437114 1106 39 -0.27500143378309616 1106 40 -0.12514568913163515 1106 41 0.03348191750195895 1106 42 0.07984120642452552 1106 43 -0.10905376139010059 1107 1 2.079035379544834 1107 2 1.0126861856397689 1107 3 -4.654407900748339 1107 4 -22.857492391957226 1107 5 -21.951203353284058 1107 6 -10.24946895718747 1107 7 -6.0973219546977155 1107 8 -1.8704068041587547 1107 9 1.314120699097245 1107 10 1.5626882122799337 1107 11 2.1917334933723502 1107 12 4.61512483688529 1107 13 2.5097536748189206 1107 14 0.5589884666027274 1107 15 1.8775612567689173 1107 16 1.9027780356478843 1107 17 1.0567040512922716 1107 18 0.4092887184924976 1107 19 -0.05457053620135355 1107 20 0.02733184379143694 1107 21 0.4725725101462359 1107 22 0.3842199807813955 1107 23 1.244424149473744 1107 24 0.7629872246500256 1107 25 0.6682040920569818 1107 26 12.287363621312284 1107 27 7.471878413222971 1107 28 3.0027000984644374 1107 29 1.367843122862439 1107 30 0.6486918598721516 1107 31 0.5663088451996288 1107 32 0.32059998522091604 1107 33 0.41292289713283736 1107 34 0.19371663197130573 1107 35 0.3472845537742444 1107 36 0.19108349496928412 1107 37 0.13845688790496524 1107 38 -0.17456150008589333 1107 39 -0.12281691530345995 1107 40 0.16894144459485716 1107 41 -0.16165193249958776 1107 42 -0.2537988343325708 1107 43 -0.016843924109707586 1113 1 -10.064026136907241 1113 2 -0.5838803379653825 1113 3 2.3308090758849467 1113 4 2.1425076080427736 1113 5 -21.951203353284058 1113 6 -10.176984455518419 1113 7 3.9026780453022845 1113 8 2.032311364653836 1113 9 -20.56570762708301 1113 10 -21.166925521625647 1113 11 -11.052901699760696 1113 12 -21.475003918479516 1113 13 -9.902263492563055 1113 14 0.215640827117749 1113 15 1.8251053674031574 1113 16 1.2003459898682003 1113 17 -0.8712883187601861 1113 18 0.011577702755730002 1113 19 -1.6082186048708849 1113 20 0.20806622624255766 1113 21 0.16451337805276722 1113 22 0.18536447291301528 1113 23 2.2291642543855232 1113 24 0.4911703433910901 1113 25 1.3138884029773479 1113 26 13.42088770333422 1113 27 7.937305213413719 1113 28 3.0418035796280023 1113 29 1.3125260031676333 1113 30 0.32012724375388757 1113 31 0.309274987307397 1113 32 1.0764416638093692 1113 33 1.834477498944949 1113 34 2.176072378275556 1113 35 0.9405129753288435 1113 36 1.5501679012639897 1113 37 1.3907220285821253 1113 38 -0.062019773810263246 1113 39 -0.23631238502211005 1113 40 -1.4834190704265993 1113 41 -1.4272694813789428 1113 42 0.22068852856681076 1113 43 -2.5509402521974565 1114 1 -2.1509826586463703 1114 2 -2.4679383089798748 1114 3 -6.823780296095727 1114 4 0.4903336949992969 1114 5 1.309666211933333 1114 6 0.11770153481974432 1114 7 1.68528674095446 1114 8 1.8439055675523868 1114 9 -3.7975916850540266 1114 10 1.2098860725772518 1114 11 1.4398519234277103 1114 12 -21.475003918479516 1114 13 -8.148640304157258 1114 14 -0.16117076708514944 1114 15 -27.397116854819064 1114 16 -14.031538068102815 1114 17 -6.470322135185306 1114 18 -3.0463933117370203 1114 19 -0.8835809237114631 1114 20 -0.2460400539506793 1114 21 -0.7147136750873315 1114 22 -0.01753407781162508 1114 23 -0.05102898232945563 1114 24 0.02740222744905907 1114 25 -0.748913529389803 1114 26 12.097216205749685 1114 27 5.888996034669756 1114 28 2.718132082043468 1114 29 0.4526226215251228 1114 30 0.1268905287780413 1114 31 -0.20280230737859029 1114 32 -0.6385341816012584 1114 33 -0.3056191194125404 1114 34 0.006990252671691621 1114 35 0.061285922188744735 1114 36 -0.4063538378664475 1114 37 -0.5561378747995178 1114 38 -0.12965262405180766 1114 39 -0.2024959599013343 1114 40 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34 -1.0461498439466723 1115 35 0.051624086439950645 1115 36 0.4946123457084326 1115 37 -0.6092779714178747 1115 38 -0.11757532936582038 1115 39 -0.40297905168877435 1115 40 -0.31675240375993496 1115 41 -0.5939361480456071 1115 42 -0.0015336936554106728 1115 43 -1.328718029975235 1201 1 6.862587479361551 1201 2 5.875005603679181 1201 3 5.515011846294616 1201 4 9.229156591243484 1201 5 8.91970734521285 1201 6 7.092983124793989 1201 7 6.333270441411923 1201 8 6.167589879242694 1201 9 6.430755679725124 1201 10 5.7915183333699325 1201 11 5.795904666287051 1201 12 9.152759123076628 1201 13 7.425764800982481 1201 14 0.143138616684503 1201 15 1.8504516686115267 1201 16 1.8174989518487479 1201 17 0.9701499364711736 1201 18 0.3396059963012661 1201 19 0.25738705383822236 1201 20 0.2060031552139705 1201 21 0.1860282616366149 1201 22 0.30826456133033986 1201 23 1.1271896006867266 1201 24 0.5875452328694237 1201 25 0.39051675546776465 1201 26 -5.1452779317969295 1201 27 -3.2256773978447555 1201 28 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2215 34 0.814961267164442 2215 35 1.0794018642177292 2215 36 0.9251679012639897 2215 37 1.5851664730265682 2215 38 1.2852024484119582 2215 39 0.7359098372001114 2215 40 1.683247596240065 2215 41 0.47550829639883574 2215 42 1.0679107507890322 2215 43 1.171281970024765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 9.0 25 122.0 26 914.0 27 3458.0 28 8381.0 29 15847.0 30 24789.0 31 33336.0 32 39773.0 33 41199.0 34 32706.0 35 22580.0 36 9615.0 37 5532.0 38 158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.1006209925783814 2.284966594301678 0.6933575671900507 95.92105484592989 2 2.126088944253604 92.25942563302422 1.5300793980345526 4.08440602468763 3 1.1769196246943405 1.7892869276358008 95.76627701651294 1.267516431156913 4 95.20034438519997 2.9660601565929734 0.9517477122678704 0.8818477459391955 5 1.7001860662812334 3.265918642626961 92.45846037742396 2.5754349136678476 6 1.1765051296462514 3.1725792515665763 1.2616497076563011 94.38926591113088 7 5.039863685129478 86.21536908092644 2.072070176914824 6.672697057029262 8 90.73857368749974 3.6536517643308626 3.776544654578704 1.8312298935906954 9 23.72285636206933 17.96640810577873 38.81902839965525 19.49170713249669 10 19.52229553501304 23.51772596882783 31.169229347702824 25.7907491484563 11 17.694661050115513 16.847128531210153 36.77340077683468 28.68480964183966 12 19.38126681340181 21.96051644023916 43.06043969944594 15.59777704691308 13 18.417686826237894 25.27077959879153 31.923310628497614 24.388222946472958 14 29.968871138259136 26.974710946283835 20.709503112886175 22.346914802570858 15 26.544163778837937 28.461710127962743 23.97901394158094 21.015112151618375 16 21.55692392933147 25.55291115224627 32.951363966437064 19.938800951985193 17 21.68661054697822 26.882505169470555 29.581954458327566 21.848929825223664 18 19.473224997273423 29.78640402023541 26.927691133166103 23.812679849325065 19 21.532679861923757 30.243395031436254 22.94280237732731 25.28112272931268 20 21.708840318934314 30.655274957113317 23.776628540510615 23.859256183441758 21 20.52520981968719 29.546722366925454 25.844836191746463 24.083231621640895 22 22.155952336013492 27.358977262718152 24.90363603571863 25.581434365549725 23 21.547615023156755 27.73148304655015 26.56941955985261 24.151482370440487 24 20.121299057541556 30.101208376849158 28.13240555492641 21.64508701068287 25 19.218267000532677 27.45334893611667 30.41703891048952 22.91134515286114 26 16.241789976743593 29.545222213703394 29.757980014822767 24.455007794730253 27 19.14460797678018 29.7415872190322 29.854414743915076 21.259390060272548 28 19.206006333494116 27.909317786213116 31.58861648805654 21.296059392236227 29 21.358254092756564 29.227503995235075 30.57812414799777 18.836117764010588 30 19.37773154711473 30.045130820659516 30.837436770713623 19.739700861512134 31 20.073484076352976 31.78354074129998 28.36854445325247 19.77443072909458 32 18.817292246003884 32.17067431706366 29.792927577080686 19.219105859851773 33 19.002260725864968 32.11237359438635 30.107080392082846 18.778285287665835 34 19.839022896665114 32.991498160800944 29.87933008694777 17.290148855586175 35 19.831473162793234 32.09937127494034 30.313439784580925 17.755715777685506 36 19.446436735327303 33.757796148796864 29.540850351691773 17.25491676418406 37 18.84371631455547 34.273694630042066 28.657112059022143 18.225476996380323 38 19.55590787646958 34.63859843384965 26.88418288810875 18.921310801572023 39 19.090340954370248 33.88781934325704 29.63648031406893 17.385359388303783 40 18.807645363834258 34.78665710367043 30.162445107143306 16.243252425352004 41 20.26684114940504 34.071948963799024 28.53799403571024 17.123215851085693 42 20.189246662388484 33.7624098750519 30.136440468251273 15.911902994308338 43 17.874833800997404 34.811822883243366 29.61215339381509 17.70118992194414 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 8.0 9 14.0 10 84.0 11 154.0 12 154.0 13 363.0 14 572.0 15 845.5 16 1119.0 17 1552.5 18 1986.0 19 1986.0 20 2711.5 21 3437.0 22 4013.0 23 4589.0 24 5039.0 25 5489.0 26 5489.0 27 5949.5 28 6410.0 29 7487.5 30 8565.0 31 9467.0 32 10369.0 33 10369.0 34 11825.5 35 13282.0 36 14610.5 37 15939.0 38 18587.5 39 21236.0 40 21236.0 41 23095.0 42 24954.0 43 24650.5 44 24347.0 45 23436.5 46 22526.0 47 22526.0 48 21427.0 49 20328.0 50 18880.0 51 17432.0 52 16172.0 53 14912.0 54 14912.0 55 11844.5 56 8777.0 57 6743.5 58 4710.0 59 3779.0 60 2848.0 61 2848.0 62 2283.5 63 1719.0 64 1444.5 65 1170.0 66 899.5 67 629.0 68 629.0 69 520.5 70 412.0 71 333.0 72 254.0 73 205.0 74 156.0 75 156.0 76 100.5 77 45.0 78 39.5 79 34.0 80 18.0 81 2.0 82 2.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.30828079976847483 2 0.0 3 0.0 4 1.5929938469668943 5 3.2950394054165146 6 4.194296595489453E-4 7 0.3078613701089259 8 0.0 9 0.23655832798560517 10 0.1350563503747604 11 0.33051057172456894 12 0.5272230820530243 13 1.2918433514107517 14 0.023068631275191993 15 0.3892307240614213 16 0.07549733871881016 17 0.0 18 0.010485741488723633 19 0.0 20 0.0 21 0.0 22 0.0 23 0.7428099270611822 24 0.0 25 0.0 26 34.35254740603727 27 8.388593190978906E-4 28 0.0016777186381957813 29 0.004194296595489454 30 4.194296595489453E-4 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 238419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.97276954776738 #Duplication Level Percentage of deduplicated Percentage of total 1 82.41279597839629 49.42523620999531 2 8.760937200573366 10.508353355048976 3 3.5173357090281403 6.328330916990329 4 1.7557311528123873 4.21184239261813 5 0.9551493833544478 2.86414769258042 6 0.6038307839866295 2.17280426723267 7 0.3532648514107129 1.483039006608668 8 0.28266412396619023 1.35617202928367 9 0.17940486820476198 0.9683466134932591 >10 1.0258305504274448 11.3480200409502 >50 0.1010877511313003 4.227535057150055 >100 0.05125576113699733 4.877760859214238 >500 7.118855713471852E-4 0.22841155883410214 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATGTCAGGGTTGGTATCAACCCCTCACGGGTTGGTGAAGAGA 535 0.22439486785868576 No Hit CATGTCAGCTTGCCCACAATAAAATATCACCTCATACCTGTTA 439 0.184129620541987 No Hit CATGTCAGTAGGCAGAGAAAAGCACTTGGGAGAAAAATAACGT 387 0.16231927824544184 No Hit CATGTCAGGGTTGGTATCAACCCCTNACGGGTTGGTGAAGAGA 364 0.1526723960758161 No Hit CATGTCAGTAGGCAGAGAAAAGCACNTGGGAGAAAAATAACGT 289 0.12121517160964519 No Hit CATGTCAGGTCAGAATGAGCTCATGGGTTTGAGGAAAGTATCC 276 0.1157625860355089 No Hit CATGTCAGCTTGCCCACAATAAAATNTCACCTCATACCTGTTA 268 0.11240714875911735 No Hit CATGTCAGGATACTGTGTCATGGCACATGCCTGTAGCCTCAGC 250 0.10485741488723632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 4.194296595489453E-4 0.0 0.0 12 0.0 0.0 0.002516577957293672 0.0 0.0 13 0.0 0.0 0.007969163531429962 0.0 0.0 14 0.0 0.0 0.03187665412571985 0.0 0.0 15 0.0 0.0 0.06333387859189074 0.0 0.0 16 0.0 0.0 0.10066311829174689 0.0 0.0 17 0.0 0.0 0.12037631229054731 0.0 0.0 18 0.0 0.0 0.14176722492754354 0.0 0.0 19 0.0 0.0 0.1543501147140119 0.0 0.0 20 0.0 0.0 0.20216509590259166 0.0 0.0 21 0.0 0.0 0.2617241075585419 0.0 0.0 22 0.0 0.0 0.3003116362370449 0.0 0.0 23 0.0 0.0 0.4303348306972179 0.0 0.0 24 0.0 0.0 0.4949269982677555 0.0 0.0 25 0.0 0.0 0.5536471506046079 0.0 0.0 26 0.0 0.0 0.583426656432583 0.0 0.0 27 0.0 0.0 0.7197412957859902 0.0 0.0 28 0.0 0.0 0.9244229696458756 0.0 0.0 29 0.0 0.0 1.051510156489206 0.0 0.0 30 0.0 0.0 1.189921944140358 0.0 0.0 31 0.0 0.0 1.3996367739148305 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTCACG 105 0.0 52.710636 23 TCACGGG 105 0.0 52.104187 25 CTCACGG 105 0.0 52.104187 24 CCCTCAC 110 0.0 50.314697 22 CACGGGT 110 0.0 49.735817 26 GGCACAT 45 4.7220965E-9 46.8539 22 CTATAAC 30 4.4694025E-5 43.92553 20 CCACTGT 120 0.0 43.92553 22 CACTGTT 125 0.0 42.16851 23 TGCAGGC 25 9.659538E-4 42.16851 21 GCCACTG 125 0.0 42.16851 21 ATCGGGT 25 0.0010225913 41.68335 24 CACAAAC 25 0.0010225913 41.68335 24 CACATGC 55 3.0770025E-8 37.893955 24 ACATGCC 55 3.0770025E-8 37.893955 25 ATCGGCG 35 1.1871381E-4 37.217274 24 TCCGGCT 35 7.677536E-7 35.54262 5 GACAGGT 30 1.0910275E-5 35.54262 4 TGCCAGG 75 0.0 35.54262 3 TCCGATT 35 7.677536E-7 35.54262 5 >>END_MODULE