##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099034_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174722 Sequences flagged as poor quality 0 Sequence length 47 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.82146495575829 33.0 27.0 37.0 17.0 39.0 2 30.69503554217557 31.0 27.0 38.0 18.0 39.0 3 24.01955105825254 26.0 18.0 31.0 10.0 34.0 4 28.243151978571674 30.0 26.0 34.0 17.0 37.0 5 19.305662709904876 18.0 8.0 30.0 2.0 34.0 6 27.121581712663545 30.0 18.0 34.0 16.0 37.0 7 26.587539062052862 30.0 18.0 36.0 7.0 39.0 8 22.84190886093337 30.0 2.0 37.0 2.0 39.0 9 31.449405341056078 32.0 30.0 35.0 26.0 37.0 10 31.06722679456508 32.0 30.0 35.0 25.0 37.0 11 31.11604148304163 32.0 30.0 35.0 25.0 37.0 12 30.61621318437289 32.0 29.0 35.0 23.0 37.0 13 30.97664861894896 32.0 28.0 37.0 23.0 39.0 14 30.34684813589588 32.0 28.0 37.0 18.0 39.0 15 30.673126452307095 32.0 28.0 37.0 18.0 39.0 16 30.482131614793786 32.0 28.0 37.0 18.0 39.0 17 30.325574340953057 31.0 27.0 35.0 18.0 39.0 18 31.117981708084844 31.0 31.0 33.0 30.0 34.0 19 31.210477215233343 31.0 31.0 34.0 30.0 34.0 20 31.34088437632353 31.0 31.0 34.0 30.0 34.0 21 35.160964274676346 35.0 35.0 37.0 33.0 37.0 22 35.157078101212214 35.0 35.0 37.0 33.0 37.0 23 34.66550863657696 35.0 35.0 37.0 32.0 37.0 24 34.6082920296242 35.0 35.0 37.0 32.0 37.0 25 34.61110220807912 35.0 35.0 37.0 32.0 37.0 26 36.172233605384555 37.0 35.0 39.0 32.0 39.0 27 35.901723881365825 37.0 35.0 39.0 32.0 39.0 28 36.38141161387804 37.0 35.0 39.0 32.0 39.0 29 36.66232071519328 37.0 35.0 39.0 33.0 39.0 30 21.471205686748092 32.0 2.0 37.0 2.0 39.0 31 28.938433625988715 34.0 17.0 38.0 17.0 40.0 32 34.15861768981582 35.0 32.0 39.0 27.0 40.0 33 36.636090475154816 37.0 35.0 40.0 32.0 40.0 34 37.566814711370064 38.0 37.0 40.0 34.0 40.0 35 38.02004326873548 39.0 37.0 40.0 34.0 41.0 36 38.33890408763636 39.0 38.0 40.0 35.0 41.0 37 38.350682798960634 40.0 38.0 40.0 35.0 41.0 38 38.23402891450419 40.0 38.0 40.0 34.0 41.0 39 38.11960142397638 39.0 37.0 40.0 34.0 41.0 40 37.91880244044825 39.0 37.0 40.0 34.0 41.0 41 37.79431325190875 39.0 37.0 40.0 33.0 41.0 42 37.58407069516146 39.0 37.0 40.0 33.0 41.0 43 37.29112533052506 39.0 36.0 40.0 32.0 41.0 44 37.086440173532814 39.0 36.0 40.0 32.0 41.0 45 36.94343013472831 39.0 36.0 40.0 32.0 41.0 46 36.768798434083855 38.0 36.0 40.0 31.0 41.0 47 36.675759206053044 38.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.241670985557182 1101 2 5.032343357164848 1101 3 2.7348185207441134 1101 4 3.61985241454871 1101 5 7.658183121198338 1101 6 6.698383629266772 1101 7 8.97735797719059 1101 8 14.583097945765868 1101 9 3.144137300613263 1101 10 3.7444683606477263 1101 11 3.6134348986916045 1101 12 3.7095925344045924 1101 13 4.937038855977423 1101 14 5.683646536363437 1101 15 4.766455745569729 1101 16 4.862140011682662 1101 17 4.254001098807368 1101 18 0.26152685492739636 1101 19 -0.389324208459076 1101 20 0.0375734681781843 1101 21 0.0752115795666839 1101 22 -0.08620666804765875 1101 23 -0.13969669783285354 1101 24 -0.09616025940327688 1101 25 -0.10122354214168183 1101 26 -0.06378545045988204 1101 27 1.297172488087348 1101 28 0.5365527652494393 1101 29 0.26201957003964793 1101 30 16.815729550057956 1101 31 9.902805706249382 1101 32 4.341239239876003 1101 33 1.7603547244615996 1101 34 0.8797560406802916 1101 35 0.326579422847189 1101 36 0.09664124275396802 1101 37 -0.13345276004017137 1101 38 -0.19951740957793618 1101 39 -0.16805142847663035 1101 40 -0.2420479492804759 1101 41 -0.5428051215298311 1101 42 -0.29915519245994915 1101 43 -0.32679792180504563 1101 44 -0.3439090659296369 1101 45 -0.16376286138132912 1101 46 -0.220479737133374 1101 47 -0.2934077440892935 1102 1 5.717913108579598 1102 2 1.8336598332396612 1102 3 2.0602391917116734 1102 4 0.11033335185346971 1102 5 7.623304461167873 1102 6 4.9031300566932785 1102 7 3.086451630446799 1102 8 8.839746430741986 1102 9 2.773014364773335 1102 10 2.5493521452473438 1102 11 2.812174582278704 1102 12 1.5396171276565411 1102 13 3.787152228294925 1102 14 4.294052921716993 1102 15 3.4467235803573644 1102 16 4.460824237603617 1102 17 3.139741926647588 1102 18 0.3538954588684007 1102 19 0.276436883659148 1102 20 0.15745211650872193 1102 21 0.010854946993610781 1102 22 -0.1428729369167243 1102 23 -0.23029276244454877 1102 24 -0.12269218968106088 1102 25 -0.12957949143886793 1102 26 -0.24027771797643283 1102 27 0.9347999295701541 1102 28 0.6059883372472967 1102 29 0.32380572808698105 1102 30 16.619813059475224 1102 31 9.751133669315045 1102 32 4.084320486526899 1102 33 1.63323030975058 1102 34 0.748569410423805 1102 35 0.34599779594858404 1102 36 0.011107737665675188 1102 37 -0.5564134038170963 1102 38 -0.439175256837693 1102 39 -0.3732927629237963 1102 40 -0.4276287220842221 1102 41 -0.6699927158605163 1102 42 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10.31561909435186 1103 32 4.6416864743456046 1103 33 2.115812702805087 1103 34 1.097796406303516 1103 35 0.7821332371484431 1103 36 0.44885948353126537 1103 37 0.3867978600305264 1103 38 0.46214549497487667 1103 39 0.2946207673421526 1103 40 0.3498189532591738 1103 41 0.21918266089240745 1103 42 0.29745378176379234 1103 43 0.1557436598499038 1103 44 0.09621304957379095 1103 45 0.023393010680685222 1103 46 0.16354971283453068 1103 47 -0.014029351782689048 1106 1 -6.4327603344503075 1106 2 -3.9622777034516616 1106 3 -1.9897029408299645 1106 4 -4.281706098657274 1106 5 -15.02939014961057 1106 6 -10.247132443516385 1106 7 -4.63216156333371 1106 8 -17.368836403589352 1106 9 -6.696760371468468 1106 10 -3.139648005711244 1106 11 -1.6527191596492017 1106 12 -3.4190537658505598 1106 13 -2.0176834784428586 1106 14 -1.4821006534855385 1106 15 -2.8955330827869368 1106 16 -1.756950297360067 1106 17 -6.477544508884709 1106 18 0.9346331431636763 1106 19 0.7941409712822214 1106 20 0.4339747015906603 1106 21 0.3427793335146916 1106 22 -0.21576916127472856 1106 23 -0.27854919076438023 1106 24 -0.34133054322607137 1106 25 0.1892785907161496 1106 26 0.20962919089490128 1106 27 1.47682512288101 1106 28 1.05882216538933 1106 29 0.9852307771485229 1106 30 17.251650225479356 1106 31 10.527949784982866 1106 32 4.4207904038351415 1106 33 2.134639256562835 1106 34 1.0705556899655662 1106 35 0.9520042703946459 1106 36 -0.0654953586626803 1106 37 0.11692898703145715 1106 38 0.18428370758640256 1106 39 -0.4131626030754276 1106 40 -0.12997193481842118 1106 41 0.08651108326392176 1106 42 -0.05912741112467046 1106 43 -0.04519172715836817 1106 44 -0.47078701523362554 1106 45 -0.740319157991415 1106 46 -0.2584869681617761 1106 47 -1.0609607207312948 1107 1 -3.8618469116099057 1107 2 -1.028087935590495 1107 3 -0.011467968680186402 1107 4 1.4349943746354903 1107 5 -15.02939014961057 1107 6 -8.149511786058 1107 7 -2.6858658036505254 1107 8 -17.368836403589352 1107 9 -1.3656164196348612 1107 10 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0.3429812053145085 1107 47 0.27900515067473464 1201 1 7.901064798656375 1201 2 6.508855788112903 1201 3 4.313972727910812 1201 4 3.8636848821625343 1201 5 9.679443001970672 1201 6 8.044804178460652 1201 7 10.789064942535813 1201 8 15.488851709823951 1201 9 4.139662747397399 1201 10 4.879147298670052 1201 11 4.470861570352721 1201 12 4.3923837862695265 1201 13 5.799751892652143 1201 14 6.129901399243952 1201 15 5.634391864291132 1201 16 5.6932710125379415 1201 17 5.2648058663798984 1201 18 0.40140458456466277 1201 19 0.45730089173895294 1201 20 0.21625708019095313 1201 21 0.33123273014051335 1201 22 0.13316277791315656 1201 23 0.1564049435611068 1201 24 0.1599203388061312 1201 25 0.03295041058913739 1201 26 0.017442392511426874 1201 27 1.3919575880808281 1201 28 0.6799973530216405 1201 29 0.36998926069460936 1201 30 -0.2162117284625289 1201 31 -0.21789664519225482 1201 32 -0.37691951982898075 1201 33 -0.21750007245120884 1201 34 -0.1296175090850511 1201 35 -0.2542629693256657 1201 36 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41 0.47748665543920055 2101 42 0.47304596598357307 2101 43 0.620961334912721 2101 44 0.3983149273769868 2101 45 0.3681778374217046 2101 46 0.12500380473412065 2101 47 0.31819206873427675 2102 1 -10.93023136077553 2102 2 -7.539082833086457 2102 3 -3.452843647349539 2102 4 -2.6170838516542076 2102 5 3.2075766750339803 2102 6 0.3875403829601751 2102 7 -21.111153159972364 2102 8 -17.368836403589352 2102 9 -1.7360291595524195 2102 10 0.019099853622062568 2102 11 -0.3198504671485054 2102 12 -0.680274841958731 2102 13 -0.42650103390106153 2102 14 0.26699329689082063 2102 15 -1.3769939135647462 2102 16 -0.39018937498163453 2102 17 -1.640394482997575 2102 18 0.7112471062844712 2102 19 0.7814363633727055 2102 20 -0.0536990408921163 2102 21 0.26276818213470676 2102 22 0.2310427388407703 2102 23 0.35903095977925403 2102 24 0.6629308769818252 2102 25 0.33775124992201455 2102 26 0.5275231731323444 2102 27 2.033597594902993 2102 28 1.2688663726456966 2102 29 0.7134955427704099 2102 30 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19 0.7393510553158364 2209 20 0.8924620965486447 2209 21 0.8110146276323391 2209 22 0.8450711748597257 2209 23 0.5285096327650294 2209 24 0.040182061815947634 2209 25 1.0393626243295984 2209 26 0.153494737113391 2209 27 1.0946402489314266 2209 28 0.5640322494229437 2209 29 0.15027279395524573 2209 30 0.8496334187566639 2209 31 1.4805548269996756 2209 32 0.7979332609779988 2209 33 0.9064039624451894 2209 34 0.19795064794875827 2209 35 -0.17236547750451336 2209 36 0.149630691757487 2209 37 0.5431474744264193 2209 38 -1.0698339394724243 2209 39 -0.61417100643677 2209 40 0.1049860483748617 2209 41 0.2061749488101441 2209 42 -0.5613122850742513 2209 43 0.7343040711609561 2209 44 0.45055752258150505 2209 45 -0.8051931075712488 2209 46 -0.4174785648004331 2209 47 0.9951737330502155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 37.0 26 349.0 27 1703.0 28 5651.0 29 13616.0 30 22869.0 31 26545.0 32 21462.0 33 18706.0 34 20208.0 35 19831.0 36 14960.0 37 8013.0 38 771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.038918968418401804 0.7182839024278569 0.17914172227882005 99.06365540687491 2 0.13850574054784173 1.8595254175204037 97.72953606300294 0.2724327789288126 3 0.6186971302984169 94.06657432950631 1.2299538695756687 4.084774670619613 4 95.95872299996566 1.0136101921910234 2.8090337793752362 0.21863302846808075 5 0.7445124299345343 89.49196297405618 1.827763752799053 7.935760843210246 6 2.449069818850275 1.8824679177671 0.34787262510034783 95.32058963828229 7 0.3581253863027644 1.570661592718723 96.7308181741059 1.3403948468726141 8 96.95577945030898 1.8651661977701364 0.8528306121303934 0.3262237397904794 9 25.305645862905813 30.170795366088193 22.858761848069967 21.664796922936034 10 28.780607276353308 28.128079679509234 21.31823403523299 21.773079008904464 11 27.242133217339543 28.93167431691487 21.814654136285068 22.011538329460514 12 26.774686500191734 28.525477761688634 22.078628212979552 22.62120752514008 13 27.08558480041834 28.439370170239965 23.156121085352392 21.31892394398931 14 25.336002287675154 28.878324277952533 23.056667619864648 22.729005814507673 15 24.324355261501125 28.491546571124417 24.647726102036376 22.53637206533808 16 28.024445144210024 27.969630173891748 22.609425842342816 21.396498839555417 17 28.82121312713911 30.37453783725003 23.066929178924234 17.73731985668662 18 29.28811851716163 29.036819087307947 20.724475076134 20.950587319396423 19 26.961578305736637 29.722010771581797 21.96956255473074 21.346848367950823 20 21.89878778860132 27.166584631586176 29.841119034809584 21.09350854500292 21 21.858151806870342 31.262233719852105 26.107759755497305 20.771854717780244 22 21.184282075498697 31.430125067971726 25.565381643341635 21.820211213187946 23 22.350934627579814 30.361946406291136 22.311443321390552 24.975675644738498 24 23.580316159384623 29.00665056489738 24.3581231899818 23.0549100857362 25 21.46552809606117 29.423312462082624 26.356726685820902 22.7544327560353 26 22.222158629136572 27.752658508945636 26.431130596032553 23.594052265885235 27 22.242681226765797 28.413684944237914 27.530785315985128 21.81284851301115 28 22.41560879568686 29.27507697943018 27.417840912993213 20.891473311889744 29 21.359645608452286 27.894025938347777 29.392406222456245 21.353922230743695 30 17.715841895276093 30.807758596063568 29.453717898665953 22.022681609994383 31 21.284913003663004 29.937042124542124 29.05563186813187 19.722413003663004 32 20.473094401391926 29.91723995833381 30.246334176577648 19.363331463696614 33 20.347985347985347 30.63301282051282 28.91311813186813 20.1058836996337 34 19.710740490607936 32.08811712320143 28.84181728689003 19.3593250993006 35 19.535032794954272 32.62897631666304 28.735362461510285 19.100628426872404 36 19.801742196174494 32.969517290324056 29.033550440127748 18.195190073373702 37 19.913920399262828 33.72271379677431 28.686141413216426 17.677224390746442 38 19.734778676984007 32.88481130023695 29.621341330799783 17.75906869197926 39 19.37535055688465 33.7742241961516 29.541214042879542 17.309211204084203 40 19.56822838566408 33.77021783175559 29.172056180675586 17.48949760190474 41 19.219674683211043 33.91044058561601 29.50744611439887 17.362438616774075 42 19.447465116012864 34.32996417165554 28.99978251164707 17.222788200684516 43 19.338148601778826 34.646466958940486 28.501276313229013 17.51410812605167 44 19.461201222513477 34.30936001190463 28.80690468286764 17.422534082714254 45 18.825333959089296 34.37804054440769 29.20124540698939 17.595380089513625 46 18.914618651343275 34.12106088529207 29.09994162154737 17.864378841817285 47 19.0811689426632 34.2235093462758 29.143439292132644 17.551882418928354 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.5 8 5.0 9 5.0 10 17.0 11 29.0 12 73.0 13 117.0 14 227.0 15 337.0 16 499.5 17 662.0 18 828.0 19 994.0 20 1248.0 21 1502.0 22 1971.5 23 2441.0 24 3003.0 25 3565.0 26 3565.0 27 4324.0 28 5083.0 29 5846.0 30 6609.0 31 7167.5 32 7726.0 33 8509.5 34 9293.0 35 10133.0 36 10973.0 37 11790.0 38 12607.0 39 13229.5 40 13852.0 41 14100.5 42 14349.0 43 14349.0 44 14387.5 45 14426.0 46 14341.0 47 14256.0 48 13779.0 49 13302.0 50 12415.0 51 11528.0 52 10584.5 53 9641.0 54 8758.5 55 7876.0 56 6616.5 57 5357.0 58 5357.0 59 4495.5 60 3634.0 61 2942.0 62 2250.0 63 1725.0 64 1200.0 65 893.0 66 586.0 67 462.0 68 338.0 69 227.5 70 117.0 71 80.0 72 43.0 73 29.0 74 15.0 75 15.0 76 10.0 77 5.0 78 4.0 79 3.0 80 2.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 19.74336374354689 6 1.613992513821957 7 5.54938702624741 8 28.59456736987901 9 0.0057233777085885 10 1.85151268872838 11 0.0 12 5.723377708588501E-4 13 1.4949462574833163 14 3.9296711347168647 15 0.0 16 1.8520850264992388 17 0.0 18 0.017170133125765503 19 0.00171701331257655 20 0.0 21 0.0 22 0.009729742104600451 23 0.0 24 0.0 25 0.0 26 0.0 27 1.4663293689403738 28 0.0 29 0.0 30 39.89365964217443 31 0.0011446755417177002 32 0.0 33 0.0011446755417177002 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 47 174722.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.13174603387598 #Duplication Level Percentage of deduplicated Percentage of total 1 91.87810151175117 79.13621305484828 2 5.573665946481434 9.601391595600038 3 1.2251899577644518 3.1658325085626897 4 0.5145516533702252 1.7727692931758088 5 0.25565493363795083 1.10100029082057 6 0.1600610103253992 0.827180057476373 7 0.09726593217883109 0.58643791977227 8 0.0694325750406247 0.4784279135901698 9 0.041649933508168195 0.3228643345728028 >10 0.17649430964358212 2.4408126208623484 >50 0.006940706760867426 0.4304269382563001 >100 9.91529537266775E-4 0.1366434724623175 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.01087441764631815 0.0 0.0 17 0.0 0.0 0.018314808667483203 0.0 0.0 18 0.0 0.0 0.04407000835613145 0.0 0.0 19 0.0 0.0 0.061812479252755806 0.0 0.0 20 0.0 0.0 0.08985703002483945 0.0 0.0 21 0.0 0.0 0.10988885200489922 0.0 0.0 22 0.0 0.0 0.13335470061011206 0.0 0.0 23 0.0 0.0 0.19173315323771478 0.0 0.0 24 0.0 0.0 0.26384771236592985 0.0 0.0 25 0.0 0.0 0.41551722164352517 0.0 0.0 26 0.0 0.0 0.4767573631254221 0.0 0.0 27 0.0 0.0 0.5236890603358478 0.0 0.0 28 0.0 0.0 0.5734824464005678 0.0 0.0 29 0.0 0.0 0.6164077792149815 0.0 0.0 30 0.0 0.0 0.7045477959272444 0.0 0.0 31 0.0 0.0 0.8224493767241675 0.0 0.0 32 0.0 0.0 0.9031490024152654 0.0 0.0 33 0.0 0.0 0.9849933036480809 0.0 0.0 34 0.0 0.0 1.0565355250054371 0.0 0.0 35 0.0 0.0 1.1240713819667816 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCATT 15 0.00411441 59.901855 26 TAGTGGC 15 0.00411441 59.901855 27 ATTGCGT 45 5.2750693E-11 54.914505 5 ACTGCGT 80 0.0 54.9145 5 ACTGCCG 105 0.0 54.9145 5 ACTGCAC 135 0.0 54.9145 5 GACTGCC 475 0.0 53.75841 4 GACTGCA 615 0.0 53.575123 4 ACTGGCG 190 0.0 53.469383 5 CGATTGC 45 7.094059E-11 53.3619 3 TCGATTG 50 3.6379788E-12 53.361897 2 CGCCTGA 85 0.0 53.361893 3 GACTGAG 805 0.0 53.20908 4 AGACTGC 1915 0.0 52.943916 3 GACTGGC 510 0.0 52.760994 4 GACTGCG 505 0.0 52.739674 4 GACTGGG 860 0.0 52.67961 4 GACTGAA 815 0.0 52.55621 4 TCGACTG 295 0.0 52.45746 2 AGACTGA 2545 0.0 52.208694 3 >>END_MODULE