FastQCFastQC Report
Thu 26 May 2016
SRR2099033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89956
Sequences flagged as poor quality0
Sequence length47
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTCGAGAGTAATCCGACGATCTCGATAATATTTTCAGTGAC1090.1211703499488639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGA600.055.071375
ACGTTCG600.055.071375
CCGTGAA150.00572632955.071375
TACGAAG150.00572632955.071374
TCCGTTG251.1643373E-555.071374
ACGTCTC600.055.071375
ACGTCCC501.8189894E-1255.071375
ACGTCAT305.290476E-755.071375
ACGTATC600.055.071375
ACGTAAA950.055.071375
TACGTAC2650.055.071374
TAAGTTG202.5761686E-455.0713654
CCGTCTC202.5761686E-455.0713655
ACGTGTC455.0931703E-1155.0713655
ACGTGTA550.055.0713655
ACGTGCT900.055.0713655
ACGTGCA1050.055.0713655
ACGTGAT750.055.0713655
ACGTCTT700.055.0713655
ACGTCGC750.055.0713655
Produced by FastQC (version 0.11.5)