FastQCFastQC Report
Thu 26 May 2016
SRR2099032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249726
Sequences flagged as poor quality0
Sequence length47
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCG150.00424063359.45944629
GTGCACG251.0870081E-555.7895774
TGCCCGT401.0059011E-955.7895775
TGCCTAA600.055.7895775
TGCCGGT202.429399E-455.7895775
TGCCGAT251.0870081E-555.7895775
GTGCCCC150.005457647655.7895774
ATGCCGC850.055.7895744
ATGCCCG900.055.7895744
TGCACGG1650.055.7895745
ATGCACG5500.054.775224
TGCACGA2250.054.549815
GATGCCG3900.054.2287863
TGCACAA3550.054.218045
TGCAGAA3450.054.172485
TGCACTA1650.054.098985
TGATGCC15950.053.5487982
ATGCAAC8450.053.4787644
ATGCAAG9600.053.465014
GATGCAC24050.053.439593
Produced by FastQC (version 0.11.5)