FastQCFastQC Report
Thu 26 May 2016
SRR2099031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136162
Sequences flagged as poor quality0
Sequence length42
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAGTGGCGATCCACGCTACGAGTTTTTTTTTTTTTTTT6700.49206092742468527No Hit
CTACGAGTGGCGATCCACGCTACGNGTTTTTTTTTTTTTTTT4130.30331516869611197No Hit
CTACGAGTCCTGGGTGTCTCCCACTGGCATCCTTTGCAGCAA3160.23207649711373218No Hit
CTACGAGTCCTGGGTGTCTCCCACNGGCATCCTTTGCAGCAA2120.15569689046870638No Hit
CTACGAATGGCATCTTTGGGTGCATTTAGACTAGGAAACCCT1470.10795963631556528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCA150.007533209451.40489622
CAAACGC250.001152787140.66081225
ACAAACG250.001152787140.66081224
ATATCAG658.54925E-1139.5422319
AGCAACT659.640644E-1139.09693524
CTACTAG650.035.0551451
CTCCGCG503.1468517E-1035.0551451
CAACGAG552.3646862E-1135.0551451
CTGCGTG200.002392938635.0551451
CTGCGCG1750.035.0551451
TTACGAG454.3764885E-935.0551451
AGTTTGT700.034.8252336
AGTGGCG2550.034.8252336
AGTGCGG1100.034.8252336
AGTGCGA700.034.8252336
AGTTGCC552.5465852E-1134.8252336
AGTTCGG454.667527E-934.8252336
AGTTCGC454.667527E-934.8252336
AGTTATC358.9652895E-734.8252336
AGTTAGA454.667527E-934.8252336
Produced by FastQC (version 0.11.5)