##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099031_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136162 Sequences flagged as poor quality 0 Sequence length 42 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.57604177376948 30.0 27.0 31.0 20.0 35.0 2 28.41659200070504 30.0 27.0 31.0 18.0 36.0 3 26.668916437772655 29.0 24.0 31.0 16.0 35.0 4 28.79827705233472 30.0 27.0 31.0 21.0 35.0 5 27.64233045930583 30.0 25.0 31.0 14.0 37.0 6 21.029450213716014 23.0 15.0 28.0 7.0 31.0 7 26.29266608892349 28.0 23.0 31.0 15.0 34.0 8 26.430582688268387 28.0 23.0 31.0 12.0 37.0 9 26.05516957741514 27.0 26.0 27.0 22.0 31.0 10 26.099895712460157 27.0 26.0 27.0 20.0 31.0 11 25.63597038821404 27.0 26.0 27.0 18.0 31.0 12 28.100989997209208 30.0 26.0 31.0 18.0 35.0 13 31.140678015892835 31.0 31.0 33.0 30.0 34.0 14 31.12976454517413 31.0 31.0 34.0 28.0 34.0 15 31.33026835681027 31.0 31.0 34.0 30.0 34.0 16 35.22721464138306 35.0 35.0 37.0 33.0 37.0 17 35.278212717204504 35.0 35.0 37.0 33.0 37.0 18 35.027959342547845 35.0 35.0 37.0 32.0 37.0 19 34.965643865395634 35.0 35.0 37.0 32.0 37.0 20 34.974611124983475 35.0 35.0 37.0 32.0 37.0 21 36.53123485260205 37.0 35.0 39.0 32.0 39.0 22 36.40928452872314 37.0 35.0 39.0 32.0 39.0 23 36.64385070724578 38.0 35.0 39.0 33.0 39.0 24 36.7985267548949 38.0 35.0 39.0 33.0 39.0 25 22.95401066376816 32.0 2.0 37.0 2.0 39.0 26 29.77141933872887 34.0 17.0 38.0 17.0 40.0 27 34.487735197779116 36.0 32.0 39.0 27.0 40.0 28 36.502702663004364 37.0 35.0 39.0 32.0 40.0 29 37.24406956419559 38.0 36.0 40.0 33.0 40.0 30 37.55861400390711 39.0 37.0 40.0 33.0 41.0 31 37.8844758449494 39.0 37.0 40.0 34.0 41.0 32 37.934607306003144 39.0 37.0 40.0 34.0 41.0 33 37.93334410481632 39.0 37.0 40.0 34.0 41.0 34 37.91092228375024 39.0 37.0 40.0 33.0 41.0 35 37.78582130109722 39.0 37.0 40.0 33.0 41.0 36 37.77553208677898 39.0 37.0 40.0 33.0 41.0 37 37.66271793892569 39.0 37.0 40.0 33.0 41.0 38 37.487074220413916 39.0 37.0 40.0 33.0 41.0 39 37.37708758684508 39.0 36.0 40.0 33.0 41.0 40 37.314889616780015 39.0 36.0 40.0 33.0 41.0 41 37.19232238069358 39.0 36.0 40.0 32.0 41.0 42 37.15720979421572 39.0 36.0 40.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.063396317550115 1101 2 6.709866462889494 1101 3 5.687273666438138 1101 4 5.399391469262923 1101 5 9.030657359714201 1101 6 6.5652022365771145 1101 7 6.437795540814232 1101 8 6.518416656317779 1101 9 6.618294456105005 1101 10 5.8995090085217115 1101 11 4.067208054568489 1101 12 8.233205698715622 1101 13 0.16914420730205038 1101 14 1.0726606839724724 1101 15 1.57428065007527 1101 16 0.692114137105861 1101 17 0.23805233495803435 1101 18 0.08252350338619863 1101 19 0.13723525043554474 1101 20 0.037822647512328444 1101 21 0.05714065643784494 1101 22 1.1161767630591726 1101 23 0.4833664025636324 1101 24 0.13087936469241868 1101 25 12.43284915274642 1101 26 7.507490714197992 1101 27 3.6294362000125417 1101 28 1.3886440804810292 1101 29 0.8282986459138755 1101 30 0.35855600204396865 1101 31 0.23559524226187278 1101 32 -0.08976219454277867 1101 33 -0.17524115819589525 1101 34 0.0012150031599063027 1101 35 -0.18647610361659872 1101 36 -0.34685961694498246 1101 37 -0.13685087872786994 1101 38 -0.4591182997665584 1101 39 -0.21899947372543238 1101 40 -0.3860393548859662 1101 41 -0.45410082235694915 1101 42 -0.28361305474341236 1102 1 4.175535473730722 1102 2 3.353069630672465 1102 3 2.4175637857716055 1102 4 1.6783838446789083 1102 5 4.5391124035540535 1102 6 3.4950313905311248 1102 7 2.471423145780143 1102 8 2.844372136449927 1102 9 4.324492213863483 1102 10 3.5154634656236468 1102 11 3.832251112104913 1102 12 5.6760303453188605 1102 13 -0.17429907169032433 1102 14 1.2918148185190006 1102 15 1.4956698047514188 1102 16 0.6853982446323528 1102 17 0.3160102462914054 1102 18 -0.09958791062209826 1102 19 0.17167526174905845 1102 20 0.21205371994888367 1102 21 -0.21441368752898882 1102 22 0.7488758176588917 1102 23 0.47872966855750576 1102 24 0.22733306053951452 1102 25 12.11536376101612 1102 26 7.041858511613938 1102 27 2.9579281932966452 1102 28 1.0660044218120532 1102 29 0.6584545677543687 1102 30 0.39847825174759066 1102 31 -0.10659933808511113 1102 32 -0.03408527952048246 1102 33 -0.2188794147116937 1102 34 0.11106437700523486 1102 35 -0.19618586392663673 1102 36 -0.15213484216207718 1102 37 -0.13500942433997665 1102 38 -0.26324712935981154 1102 39 -0.12738711792756163 1102 40 -0.42765261335755866 1102 41 -0.11236660560693679 1102 42 -0.21448028347491999 1103 1 2.3460924946124884 1103 2 0.9300166321980257 1103 3 -0.22480858315699237 1103 4 0.434676349274703 1103 5 2.4393289011579853 1103 6 0.7665812423188818 1103 7 -2.291065429888029 1103 8 -0.31742767798069593 1103 9 2.6772825308952086 1103 10 1.7984637123544651 1103 11 -1.1303431060537825 1103 12 2.5010321697368703 1103 13 -0.42009856641341514 1103 14 1.4326008125488094 1103 15 1.8707423470866047 1103 16 1.03352847680258 1103 17 0.3955583466378698 1103 18 0.2805408759140491 1103 19 0.38977487844751835 1103 20 0.2600585016616108 1103 21 0.17452790676910013 1103 22 1.209689056330994 1103 23 0.7363722424811883 1103 24 0.610881327689917 1103 25 12.555364734039053 1103 26 7.604585477902162 1103 27 3.423738599776982 1103 28 1.519526938605388 1103 29 0.8247407129502378 1103 30 0.39644185373950336 1103 31 0.2577525765112725 1103 32 -0.23271748727478325 1103 33 0.25394370709339853 1103 34 0.22720509002255085 1103 35 -0.11529290687517602 1103 36 0.06021698903234096 1103 37 -0.2077150883760055 1103 38 -0.0955466258204396 1103 39 -0.11169712303593116 1103 40 -0.10827520903525567 1103 41 -0.22815981043174105 1103 42 -0.2591246609661155 1104 1 1.4217901128966233 1104 2 0.9742293692155783 1104 3 -0.7822583606309443 1104 4 -0.7234140495102999 1104 5 1.1332190370697788 1104 6 -0.33501255866566026 1104 7 -0.7938721123646744 1104 8 -0.301613000070855 1104 9 2.0762049621552734 1104 10 1.099189256512581 1104 11 0.9180382503860827 1104 12 2.8191298979626183 1104 13 0.20506749122840873 1104 14 1.9953679583306823 1104 15 1.885855794848485 1104 16 0.9154645767648759 1104 17 0.1759112169243835 1104 18 -0.06517263457092781 1104 19 -0.07040828709565261 1104 20 -0.0471648736163246 1104 21 -0.03858967789827972 1104 22 0.7224821018193381 1104 23 0.3453818165631546 1104 24 0.09040998826985458 1104 25 12.477147620499679 1104 26 7.322483056015329 1104 27 2.668108496918144 1104 28 1.0662010286799841 1104 29 0.07208819539305722 1104 30 -0.190776729211791 1104 31 -0.22311159274242698 1104 32 -0.4062983771675519 1104 33 -0.4380083555451293 1104 34 -0.4120278558135908 1104 35 -0.32207475382376316 1104 36 -0.3385363012875118 1104 37 -0.4920868178773574 1104 38 -0.6399337894325896 1104 39 -0.4992553973754923 1104 40 -0.8160630922011478 1104 41 -0.4490543545792889 1104 42 -0.5666546041981917 1105 1 0.5491106032643991 1105 2 0.7574991745107233 1105 3 -2.4255226716092757 1105 4 -0.6383894967820289 1105 5 -1.621621112650356 1105 6 -0.9292091865287126 1105 7 -1.2053827060942872 1105 8 -4.832017175751638 1105 9 1.6744261770860795 1105 10 1.201544944847459 1105 11 0.9082137251293965 1105 12 2.99269193223342 1105 13 0.3003732842987006 1105 14 1.9232584840681213 1105 15 1.5201643694166656 1105 16 0.7771588867130248 1105 17 0.22832356540143195 1105 18 0.26068842789258895 1105 19 0.2685258154420396 1105 20 0.1941729925283795 1105 21 -0.05551904158770782 1105 22 0.9631194425699974 1105 23 0.5758443693601052 1105 24 0.635511461433552 1105 25 12.468040790377081 1105 26 7.197085576533169 1105 27 3.2550835321161102 1105 28 0.8328539595546118 1105 29 0.43682498327809327 1105 30 0.3874348706645847 1105 31 0.15652166161009973 1105 32 0.2125265258234137 1105 33 -0.12855126272550876 1105 34 0.006086219939305693 1105 35 -0.07557222987198031 1105 36 -0.05459344218239437 1105 37 -0.43145359786430504 1105 38 -0.3517465705870819 1105 39 -0.210288672331707 1105 40 -0.30877625452387036 1105 41 -0.5067687187838104 1105 42 -0.3309793183220151 1106 1 -3.2862277426002606 1106 2 -4.1761290136066265 1106 3 -1.8849801388066822 1106 4 -6.011085943781879 1106 5 -0.7383688643826254 1106 6 0.003829292020601116 1106 7 -1.904218029697411 1106 8 -4.214635486233725 1106 9 -0.14636108518725166 1106 10 0.242072734910856 1106 11 0.6772392300682135 1106 12 3.0581644370248107 1106 13 0.3091894423408661 1106 14 1.9369250033073975 1106 15 1.6846785961599942 1106 16 0.7575215580973662 1106 17 -0.04720348752500314 1106 18 0.053670884032939625 1106 19 0.31786976354759844 1106 20 0.03834223917441193 1106 21 -0.21592004409397703 1106 22 0.6458851804440968 1106 23 0.3178611760827934 1106 24 0.026533133260180364 1106 25 12.289594675088864 1106 26 6.690539800327947 1106 27 2.8703127817106875 1106 28 1.1487761178916287 1106 29 0.30170777850144503 1106 30 0.31338209165824793 1106 31 0.03151797592530414 1106 32 0.1375744397258174 1106 33 -0.021533718865860862 1106 34 0.1438276322937213 1106 35 -0.36249542020074443 1106 36 0.1491364708354439 1106 37 -0.13012675133622054 1106 38 -0.0514222303246612 1106 39 -0.4834716297251873 1106 40 -0.2950802498061975 1106 41 0.10167887077832916 1106 42 -0.10059453282887176 1107 1 2.1122684980012423 1107 2 1.9805125904033964 1107 3 -0.1293410410623217 1107 4 -4.024870404934763 1107 5 -23.490248563630747 1107 6 -17.76058173554832 1107 7 -8.503215523431749 1107 8 -6.156991375958036 1107 9 1.506521120326532 1107 10 1.867386018118875 1107 11 2.204808152373978 1107 12 4.071948898177691 1107 13 0.15630723682708236 1107 14 1.83667437674098 1107 15 1.6658816036787911 1107 16 1.1196769465685463 1107 17 0.06557846736221506 1107 18 0.29051298929609715 1107 19 -0.11320793068799162 1107 20 0.1172896075954597 1107 21 -0.3186769363245503 1107 22 0.6458851804440968 1107 23 -0.18339195674928277 1107 24 0.28969102799702284 1107 25 12.290847807920947 1107 26 6.948685163736471 1107 27 2.856528320557807 1107 28 1.1111821329292226 1107 29 0.5761438687270086 1107 30 -0.014938710346768858 1107 31 -0.05369505665615293 1107 32 -0.26342806653984496 1107 33 0.39951891271308426 1107 34 0.2866847751508601 1107 35 0.061063477042367253 1107 36 0.7619184257226621 1107 37 0.33603866219761613 1107 38 0.43855270701870097 1107 39 0.7546236083700464 1107 40 0.9179523316474416 1107 41 0.9187214772946177 1107 42 0.4921372967450637 1113 1 -11.4008893967356 1113 2 -7.2322067078422165 1113 3 1.9298694852534695 1113 4 0.10670854243365824 1113 5 2.7597514363692532 1113 6 -17.76058173554832 1113 7 -10.218127804133502 1113 8 -2.6109387443790872 1113 9 -21.20400519546294 1113 10 -21.772964859074108 1113 11 -12.290805882713741 1113 12 -22.287700224629326 1113 13 1.4984124999849762 1113 14 2.950709464460278 1113 15 2.3961447615735274 1113 16 0.8894137886738065 1113 17 1.315578467362215 1113 18 0.06902176122591897 1113 19 1.2003885605400768 1113 20 1.0887808356656379 1113 21 0.6111476250789565 1113 22 1.8827272857072543 1113 23 1.996432604654224 1113 24 0.6252173437864954 1113 25 13.418040790377084 1113 26 9.01228165496454 1113 27 4.64380902231219 1113 28 3.051971606613435 1113 29 1.1353543950427962 1113 30 1.7065525177234093 1113 31 1.3344628380806896 1113 32 1.4076245650391002 1113 33 2.0464487372744884 1113 34 0.19677249444910672 1113 35 1.9141336524809631 1113 36 0.40226930291564145 1113 37 -0.5236104606094045 1113 38 1.3727632333344886 1113 39 0.539711327668293 1113 40 1.4201453141035785 1113 41 0.2937214772946177 1113 42 0.42196185814857046 1114 1 -9.465175111021313 1114 2 -2.464349564985074 1114 3 -0.973701943317959 1114 4 -6.92186288613777 1114 5 -0.9473914207736023 1114 6 0.2679896930231074 1114 7 -0.7252706612763582 1114 8 1.464061255620912 1114 9 -5.375433766891511 1114 10 0.6270351409258907 1114 11 1.6306226887148298 1114 12 -22.287700224629326 1114 13 -0.315873214300737 1114 14 -27.549290535539722 1114 15 -13.728855238426473 1114 16 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1115 11 -9.707472549380407 1115 12 -22.287700224629326 1115 13 -0.25158750001502383 1115 14 1.0340427977936102 1115 15 -27.728855238426473 1115 16 -15.485586211326194 1115 17 -4.601088199304453 1115 18 -1.4726449054407453 1115 19 -0.9246114394599232 1115 20 0.21378083566563788 1115 21 -0.8888523749210435 1115 22 0.38272728570725434 1115 23 -0.0869007286791117 1115 24 -0.3331159895468403 1115 25 12.418040790377084 1115 26 7.137281654964539 1115 27 2.0188090223121904 1115 28 0.42697160661343503 1115 29 0.4270210617094605 1115 30 -1.085114148943255 1115 31 -0.45720382858597475 1115 32 -0.42570876829423554 1115 33 -0.7452179293921759 1115 34 -0.5948941722175576 1115 35 0.12246698581429882 1115 36 -1.7643973637510229 1115 37 -2.1069437939427402 1115 38 -1.2939034333321757 1115 39 -0.8769553389983713 1115 40 0.21181198077024277 1115 41 1.5020548106279534 1115 42 1.3386285248152348 1201 1 8.3239428180295 1201 2 7.755209399540334 1201 3 6.724332572501794 1201 4 6.402010555856478 1201 5 10.060087006839051 1201 6 7.272975311431544 1201 7 7.54697286700744 1201 8 7.8915780341444055 1201 9 7.166800173664576 1201 10 6.66495460401314 1201 11 6.744429016615118 1201 12 9.708944070672686 1201 13 0.31384874159571297 1201 14 2.0866155047287336 1201 15 1.9036950971439985 1201 16 0.9909238557878979 1201 17 0.23336370226154202 1201 18 0.3601291437762555 1201 19 0.2951872182581994 1201 20 0.24566003029651284 1201 21 0.248731517696406 1201 22 1.0941366816804106 1201 23 0.582003074452885 1201 24 0.6294119746589786 1201 25 -5.372227665998754 1201 26 -3.3643961973844547 1201 27 -0.9962916488287519 1201 28 -0.5210149705677765 1201 29 -0.016491242541093243 1201 30 0.15034446403213764 1201 31 0.06852324076525207 1201 32 0.15259100799212177 1201 33 0.11775746210670235 1201 34 0.2882154474692413 1201 35 0.05926788066888378 1201 36 0.27139681969416785 1201 37 0.22974524408858343 1201 38 0.25699142125395014 1201 39 0.1873623343796993 1201 40 0.12736007920425152 1201 41 0.2626812088382451 1201 42 0.2424316568062892 1202 1 4.945001014223305 1202 2 4.2318343880481954 1202 3 3.941855786623332 1202 4 3.57074963832407 1202 5 5.698107600752817 1202 6 3.6760621000681155 1202 7 2.759611921893896 1202 8 3.8205681049359796 1202 9 4.763460557961718 1202 10 3.970185825857399 1202 11 4.12186535016297 1202 12 6.337299775370674 1202 13 0.04122071916305714 1202 14 1.8839286425424682 1202 15 1.7728570903406506 1202 16 0.9681809119614755 1202 17 0.38064696051289815 1202 18 0.38237792560948236 1202 19 0.5582652728688444 1202 20 0.10761645210399706 1202 21 0.37998324151731566 1202 22 1.2388916692688952 1202 23 0.5700627416405268 1202 24 0.43001186433443905 1202 25 -3.5922331822256552 1202 26 -2.254841632706693 1202 27 -0.6370128954960279 1202 28 -0.10898729749615654 1202 29 0.02576535394690893 1202 30 -6.392630985061487E-4 1202 31 5.587284916472868E-4 1202 32 0.07714511298430438 1202 33 0.2193939427539391 1202 34 0.48786838486006445 1202 35 0.1042021456316462 1202 36 0.17110491935400063 1202 37 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2.86520552919983 2215 8 4.014061255620913 2215 9 -21.20400519546294 2215 10 -10.356298192407442 2215 11 -1.540805882713741 2215 12 -22.287700224629326 2215 13 0.915079166651644 2215 14 -27.549290535539722 2215 15 -13.728855238426473 2215 16 -3.3189195446595257 2215 17 -0.6010881993044492 2215 18 0.36068842789258326 2215 19 -0.9246114394599232 2215 20 0.2971141689989736 2215 21 -0.3888523749210435 2215 22 0.38272728570725434 2215 23 1.4130992713208883 2215 24 1.083550677119831 2215 25 9.75137412371042 2215 26 6.3039483216312036 2215 27 3.6854756889788547 2215 28 1.0103049399467707 2215 29 1.2603543950427962 2215 30 1.4982191843900736 2215 31 2.0427961714140253 2215 32 0.7409578983724359 2215 33 0.2547820706078241 2215 34 -0.17822750555089328 2215 35 0.7891336524809631 2215 36 1.7356026362489771 2215 37 1.6430562060572598 2215 38 0.7894299000011529 2215 39 0.2063779943349573 2215 40 1.1284786474369142 2215 41 0.835388143961282 2215 42 0.9219618581485705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 13.0 25 82.0 26 550.0 27 1798.0 28 4224.0 29 7408.0 30 11730.0 31 16644.0 32 21671.0 33 25006.0 34 21278.0 35 15458.0 36 6535.0 37 3639.0 38 126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.8129175946547884 1.5025983667409057 0.8886414253897549 96.79584261321456 2 0.9753787177988116 1.146329009006342 97.42316487452544 0.4551273986693999 3 4.214097912780365 91.73411083855994 0.8372379959166287 3.2145532527430563 4 1.0906126525756086 1.743511405531646 1.1868215801765545 95.97905436171618 5 93.7218691588785 4.20785046728972 1.223177570093458 0.8471028037383178 6 3.3579588728103578 87.5110434120335 2.3335872048743336 6.7974105102817965 7 95.04850875067015 3.4077305542703122 0.5089563090752859 1.0348043859842393 8 1.847918593894542 2.3067530064754855 94.76928769657724 1.076040703052729 9 32.68774761771507 17.255548846046217 23.451698896858478 26.605004639380237 10 28.73654549333804 17.205582654630263 27.12457971910154 26.93329213293016 11 26.64337320857515 16.54477081606064 27.89663034466422 28.915225630699986 12 28.599589504304294 18.805280333121686 30.73106625518657 21.864063907387447 13 28.887844082190373 29.805396589848886 21.069914709490682 20.23684461847006 14 25.736773451836132 32.378973123152996 22.569329294805186 19.314924130205686 15 20.1761220799459 26.96814219138207 32.52965995795416 20.326075770717868 16 21.727794832625843 29.88058342268768 28.010751898473877 20.380869846212597 17 20.266041838053823 32.02197673052062 27.296098248912916 20.415883182512633 18 21.279064643586317 30.543764045769013 23.79004421204154 24.387127098603134 19 22.70310365593925 30.063453827058943 26.643263171810048 20.590179345191757 20 20.95885783111294 29.677883697360496 26.45965834814412 22.903600123382443 21 19.81463257002688 29.212261864543702 28.10475756819083 22.868347997238583 22 20.667184437294278 30.710455268316135 27.946299789193386 20.676060505196197 23 20.236923664458512 31.02407426447908 27.36372849987515 21.37527357118726 24 20.617352859094314 30.38219180094299 28.878835504766382 20.12161983519631 25 17.971317934170834 32.270701067134944 28.583006889099018 21.174974109595208 26 20.92816592122561 32.120063748062954 27.707640220033635 19.2441301106778 27 20.163481734992143 31.756290301258794 29.74398143388023 18.336246529868834 28 19.1611401208863 32.60992501523931 28.86362467960723 19.365310184267155 29 20.098118417767072 32.60013807082739 29.017640751457822 18.28410275994771 30 19.59063468515445 32.28507219341667 30.124410628516035 17.999882492912853 31 19.77497392811504 32.35190434923106 29.166727868274556 18.706393854379343 32 19.41731173161381 33.10982506132401 29.117521775532083 18.35534143153009 33 19.26455251832376 33.10468412626136 29.44727603883609 18.18348731657878 34 20.17302918582277 32.77713312084135 29.268812150232808 17.78102554310307 35 19.537022076643996 33.138467413815896 29.183619512051823 18.140890997488285 36 20.006316005934107 34.43838956537066 28.53218959768511 17.02310483101012 37 19.741925059855173 33.53138173646098 28.535127274863765 18.191565928820083 38 18.831245134472173 33.73775355826149 29.160852513917245 18.2701487933491 39 20.579897475066463 32.899046723755525 28.560831950176997 17.960223851001015 40 19.429062440328433 32.419470924340125 28.964028142947374 19.18743849238407 41 19.62294913411965 33.21264376257693 29.645569248395294 17.518837854908124 42 20.467531322982918 33.14507718746787 28.83109825061324 17.556293238935975 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.5 7 2.0 8 5.5 9 9.0 10 9.0 11 49.5 12 90.0 13 205.0 14 320.0 15 468.0 16 616.0 17 616.0 18 866.5 19 1117.0 20 1478.0 21 1839.0 22 1839.0 23 2217.5 24 2596.0 25 2994.5 26 3393.0 27 3796.5 28 4200.0 29 4200.0 30 5069.0 31 5938.0 32 6875.0 33 7812.0 34 7812.0 35 8759.5 36 9707.0 37 10683.0 38 11659.0 39 11288.0 40 10917.0 41 10917.0 42 10996.5 43 11076.0 44 11119.0 45 11162.0 46 10994.5 47 10827.0 48 10827.0 49 10542.5 50 10258.0 51 9760.0 52 9262.0 53 9262.0 54 8656.5 55 8051.0 56 7363.5 57 6676.0 58 5315.5 59 3955.0 60 3955.0 61 3076.5 62 2198.0 63 1720.5 64 1243.0 65 922.0 66 601.0 67 601.0 68 470.0 69 339.0 70 229.0 71 119.0 72 119.0 73 98.5 74 78.0 75 65.0 76 52.0 77 41.5 78 31.0 79 31.0 80 21.5 81 12.0 82 7.0 83 2.0 84 2.0 85 3.0 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 1.073721008798343 2 0.7601239699769393 3 0.0 4 0.0 5 1.771419338728867 6 3.5707466106549552 7 7.344192946637094E-4 8 0.7615928085662667 9 0.27026630043624505 10 0.17699505001395396 11 0.7887663224688239 12 0.5258442149792159 13 0.028642352491884668 14 0.34444264919727974 15 0.08813031535964512 16 0.0 17 0.013219547303946769 18 0.0 19 0.0 20 0.0 21 0.0 22 0.710183457939807 23 0.0 24 0.0 25 34.75712753925471 26 7.344192946637094E-4 27 0.0 28 7.344192946637094E-4 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 136162.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.53183218109093 #Duplication Level Percentage of deduplicated Percentage of total 1 89.24592321840267 70.08645865034126 2 7.060921666624729 11.090142307344044 3 1.7392313177634937 4.097550659121009 4 0.6366755799729592 1.999971992009407 5 0.3557983581777722 1.3970748477362247 6 0.2108794153200371 0.9936448112615839 7 0.13692164374001844 0.7526895283705174 8 0.09794394559474628 0.6153373998880413 9 0.08994852114252389 0.6357439950571796 >10 0.39177578084129633 5.374783798323506 >50 0.025985128320979087 1.3091617078823388 >100 0.006995996086417447 1.1215779859495578 >500 9.994280123453495E-4 0.5258623167153279 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTACGAGTGGCGATCCACGCTACGAGTTTTTTTTTTTTTTTT 670 0.49206092742468527 No Hit CTACGAGTGGCGATCCACGCTACGNGTTTTTTTTTTTTTTTT 413 0.30331516869611197 No Hit CTACGAGTCCTGGGTGTCTCCCACTGGCATCCTTTGCAGCAA 316 0.23207649711373218 No Hit CTACGAGTCCTGGGTGTCTCCCACNGGCATCCTTTGCAGCAA 212 0.15569689046870638 No Hit CTACGAATGGCATCTTTGGGTGCATTTAGACTAGGAAACCCT 147 0.10795963631556528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.344192946637094E-4 0.0 0.0 0.0 0.0 7 7.344192946637094E-4 0.0 0.0 0.0 0.0 8 7.344192946637094E-4 0.0 0.0 0.0 0.0 9 7.344192946637094E-4 0.0 0.0 0.0 0.0 10 7.344192946637094E-4 0.0 0.0 0.0 0.0 11 7.344192946637094E-4 0.0 0.0 0.0 0.0 12 7.344192946637094E-4 0.0 7.344192946637094E-4 0.0 0.0 13 7.344192946637094E-4 0.0 0.005875354357309675 0.0 0.0 14 7.344192946637094E-4 0.0 0.016157224482601606 0.0 0.0 15 7.344192946637094E-4 0.0 0.02276699813457499 0.0 0.0 16 7.344192946637094E-4 0.0 0.027173513902557248 0.0 0.0 17 7.344192946637094E-4 0.0 0.03304886825986692 0.0 0.0 18 7.344192946637094E-4 0.0 0.04479957697448628 0.0 0.0 19 7.344192946637094E-4 0.0 0.0624256400464153 0.0 0.0 20 0.0014688385893274188 0.0 0.06683215581439755 0.0 0.0 21 0.0014688385893274188 0.0 0.07931728382368061 0.0 0.0 22 0.0014688385893274188 0.0 0.13513315021812253 0.0 0.0 23 0.0014688385893274188 0.0 0.17111969565664428 0.0 0.0 24 0.0014688385893274188 0.0 0.20563740250583865 0.0 0.0 25 0.0014688385893274188 0.0 0.22326346557776766 0.0 0.0 26 0.0022032578839911283 0.0 0.27026630043624505 0.0 0.0 27 0.0022032578839911283 0.0 0.3025807494014483 0.0 0.0 28 0.0022032578839911283 0.0 0.3657408087425273 0.0 0.0 29 0.0022032578839911283 0.0 0.4061338699490313 0.0 0.0 30 0.0029376771786548375 0.0 0.44726135045019905 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAACCA 15 0.0075332094 51.404896 22 CAAACGC 25 0.0011527871 40.660812 25 ACAAACG 25 0.0011527871 40.660812 24 ATATCAG 65 8.54925E-11 39.54223 19 AGCAACT 65 9.640644E-11 39.096935 24 CTACTAG 65 0.0 35.055145 1 CTCCGCG 50 3.1468517E-10 35.055145 1 CAACGAG 55 2.3646862E-11 35.055145 1 CTGCGTG 20 0.0023929386 35.055145 1 CTGCGCG 175 0.0 35.055145 1 TTACGAG 45 4.3764885E-9 35.055145 1 AGTTTGT 70 0.0 34.825233 6 AGTGGCG 255 0.0 34.825233 6 AGTGCGG 110 0.0 34.825233 6 AGTGCGA 70 0.0 34.825233 6 AGTTGCC 55 2.5465852E-11 34.825233 6 AGTTCGG 45 4.667527E-9 34.825233 6 AGTTCGC 45 4.667527E-9 34.825233 6 AGTTATC 35 8.9652895E-7 34.825233 6 AGTTAGA 45 4.667527E-9 34.825233 6 >>END_MODULE