FastQCFastQC Report
Thu 26 May 2016
SRR2099030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436660
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCAAAAACGACTCACTATAGTGAGTCGTATTACCGGC5330.12206293225850776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCGC150.007374856651.7115722
GGACCCG203.5389778E-451.7115720
GACCCGG250.001062795941.3692621
GGCCGAT900.040.22011619
TCACGCG2050.034.9290926
TCACGAT601.8189894E-1234.929096
TCCCGTC301.2316579E-534.929096
TCCCCGC200.002441816534.929096
CTCACGA3600.034.8363535
CTCACGC7050.034.836355
CTCACCG4350.034.836355
GCGCATA200.002473873634.836354
TACGCAA251.7563197E-434.836353
CTCGGTC650.034.836355
CTCGCTA406.497066E-834.836355
GCTCGCG800.034.836354
GCTCACG21700.034.836354
TGCTCAC85800.034.613043
CTCACGT6300.034.559875
TGCTCAG97750.034.5512433
Produced by FastQC (version 0.11.5)