##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099030_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 436660 Sequences flagged as poor quality 0 Sequence length 42 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.14678926395823 30.0 26.0 31.0 18.0 35.0 2 24.348234324188155 27.0 18.0 30.0 10.0 33.0 3 27.36414372738515 29.0 25.0 31.0 17.0 35.0 4 27.994856410021526 30.0 26.0 31.0 17.0 35.0 5 28.82899509916182 30.0 27.0 31.0 18.0 38.0 6 27.937571565978107 30.0 27.0 31.0 13.0 39.0 7 29.488533412723857 30.0 28.0 32.0 18.0 39.0 8 23.50293821279714 26.0 17.0 30.0 8.0 34.0 9 26.21441396051848 27.0 26.0 28.0 22.0 31.0 10 26.277082856226812 27.0 26.0 27.0 22.0 31.0 11 25.6418655246645 27.0 26.0 27.0 18.0 31.0 12 28.025770622452253 30.0 26.0 31.0 18.0 35.0 13 31.152356524527093 31.0 31.0 33.0 30.0 34.0 14 31.12821188109742 31.0 31.0 34.0 30.0 34.0 15 31.325026336279944 31.0 31.0 34.0 30.0 34.0 16 35.23745019007924 35.0 35.0 37.0 33.0 37.0 17 35.278156918426234 35.0 35.0 37.0 33.0 37.0 18 34.77065451380937 35.0 35.0 37.0 32.0 37.0 19 34.65084734118078 35.0 35.0 37.0 32.0 37.0 20 34.54018916319333 35.0 35.0 37.0 32.0 37.0 21 35.90873677460725 37.0 35.0 39.0 32.0 39.0 22 35.76168872807219 37.0 35.0 39.0 32.0 39.0 23 36.05174506481015 37.0 35.0 39.0 32.0 39.0 24 36.18946548802272 37.0 35.0 39.0 32.0 39.0 25 22.268962121559106 32.0 2.0 37.0 2.0 39.0 26 29.217700270233134 34.0 17.0 38.0 17.0 40.0 27 34.01409792515916 35.0 32.0 39.0 27.0 40.0 28 36.28391654834425 37.0 34.0 39.0 32.0 40.0 29 37.256835982228736 38.0 36.0 40.0 33.0 40.0 30 37.76398112948289 39.0 37.0 40.0 34.0 41.0 31 38.20019694957175 39.0 38.0 40.0 34.0 41.0 32 38.33297531260019 40.0 38.0 40.0 34.0 41.0 33 38.337159345944215 40.0 38.0 40.0 34.0 41.0 34 38.34048000732836 40.0 38.0 40.0 34.0 41.0 35 38.28902120643063 40.0 38.0 40.0 34.0 41.0 36 38.299370219392664 40.0 38.0 40.0 34.0 41.0 37 38.24479915723904 40.0 38.0 40.0 34.0 41.0 38 38.13337150185499 40.0 38.0 40.0 34.0 41.0 39 38.05217789584574 40.0 37.0 40.0 34.0 41.0 40 38.04439151742775 40.0 37.0 40.0 34.0 41.0 41 37.94991526588192 40.0 37.0 40.0 34.0 41.0 42 37.90304126780561 39.0 37.0 40.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.999353601078557 1101 2 5.947339636735041 1101 3 5.479622696794202 1101 4 5.671791387727428 1101 5 8.38991714022238 1101 6 9.537998485497571 1101 7 7.328721866792193 1101 8 5.937355000504073 1101 9 6.213864552704795 1101 10 5.7297467032251035 1101 11 3.7769206414572345 1101 12 8.353658085786215 1101 13 0.2409170543591017 1101 14 1.0266076880854627 1101 15 1.6638679937963978 1101 16 0.7634343820026075 1101 17 0.32904359679249495 1101 18 0.07265703021031555 1101 19 0.0902453902393745 1101 20 -0.0031428626961584882 1101 21 -0.0825762975151818 1101 22 0.9496096001874292 1101 23 0.4684532770875194 1101 24 0.22672858936071805 1101 25 11.933304942760888 1101 26 6.84204256890289 1101 27 2.9994955185277377 1101 28 1.261650411357543 1101 29 0.7444121397436376 1101 30 0.40161776183150977 1101 31 0.26886591542456273 1101 32 0.06980984627085007 1101 33 0.08703199434665265 1101 34 0.18864084254736468 1101 35 0.15228486923768259 1101 36 0.02106361329855133 1101 37 -0.002672355728257969 1101 38 -0.1429305116111621 1101 39 -0.06918740553507519 1101 40 0.03862400703119562 1101 41 -0.19266494289726666 1101 42 -0.10489595365296367 1102 1 4.415776355606059 1102 2 2.908370388703684 1102 3 2.1350395771219794 1102 4 1.4394507958396083 1102 5 4.43573951549083 1102 6 5.277590121997829 1102 7 3.456644052684979 1102 8 3.214315647935315 1102 9 4.309353933959141 1102 10 3.5210333281241972 1102 11 3.7883990028753693 1102 12 5.806494405298 1102 13 -0.14608424791594743 1102 14 1.359910770390261 1102 15 1.7180501244503787 1102 16 0.8721806947618589 1102 17 0.36664075971142296 1102 18 0.35497972533579514 1102 19 0.2748663596600238 1102 20 -0.04855970494571693 1102 21 0.09498052787841971 1102 22 0.8597529392917309 1102 23 0.4412803435923962 1102 24 0.2357623292001776 1102 25 12.021137111746942 1102 26 6.848853492781991 1102 27 2.9290765185125025 1102 28 1.3024229803171963 1102 29 0.6349464659876531 1102 30 0.5175627616538137 1102 31 0.18140310515055091 1102 32 0.0693830529137216 1102 33 0.08411710677894035 1102 34 0.13271472952106222 1102 35 0.1214735620378562 1102 36 -0.04041208264433749 1102 37 -0.0404808527556213 1102 38 -0.24433153616907077 1102 39 -0.06178880782890417 1102 40 -0.07768432245200785 1102 41 -0.1289565941210995 1102 42 -0.18789123703106014 1103 1 1.4046156405310342 1103 2 -0.8262014131814226 1103 3 -0.3908429068637993 1103 4 0.2550284836033825 1103 5 2.287240688600022 1103 6 1.9593166699803817 1103 7 0.03492588531668517 1103 8 -1.0306023228244747 1103 9 2.2682047643294965 1103 10 1.3245736956335818 1103 11 -1.3504561062052183 1103 12 1.8086277490039642 1103 13 -0.5309199330665244 1103 14 1.3454992963745838 1103 15 1.6197646142495472 1103 16 0.8506870712777541 1103 17 0.3094390008432626 1103 18 0.0986814461906178 1103 19 0.1954920414689667 1103 20 0.10724015749905647 1103 21 0.11889144583857103 1103 22 0.7792703997887998 1103 23 0.42716484316894565 1103 24 0.21654561606295175 1103 25 11.91371390310324 1103 26 7.104266680049825 1103 27 3.1520715803151163 1103 28 1.2373285923230455 1103 29 0.6758184034831203 1103 30 0.35410259344038764 1103 31 0.22286062480697666 1103 32 0.24170545549792166 1103 33 0.17908361084283086 1103 34 0.09813391051934417 1103 35 -0.01828149064155582 1103 36 -0.04376220667389674 1103 37 0.002107519407303471 1103 38 -0.05318493022944892 1103 39 -7.772287903407005E-4 1103 40 0.11890473218287667 1103 41 0.2040320849773991 1103 42 0.011591428004280147 1104 1 1.3272541663644475 1104 2 0.7734859108935481 1104 3 -0.16291506615640472 1104 4 -0.46314616311226686 1104 5 1.0613620943508195 1104 6 0.7794233096823362 1104 7 -1.0730925310084416 1104 8 -0.041269161606329874 1104 9 2.161015728066978 1104 10 1.2674472304133992 1104 11 0.8395145983554748 1104 12 2.811930926322052 1104 13 0.2837543210113509 1104 14 1.885207704560095 1104 15 1.8898266115745876 1104 16 0.8273199244814222 1104 17 0.24099954773927124 1104 18 -0.049027449654595046 1104 19 0.0735545264537123 1104 20 -0.10074670437492017 1104 21 -0.20504332250514068 1104 22 0.8123276316745773 1104 23 0.19724154199963806 1104 24 0.13510967339165347 1104 25 11.667321329873605 1104 26 6.69195865123487 1104 27 2.7369548467745233 1104 28 0.9503512577418718 1104 29 0.3191046561410502 1104 30 0.14969357603882827 1104 31 -0.019972391188971983 1104 32 -0.010733739433284484 1104 33 0.057057169647059425 1104 34 0.004290972799942949 1104 35 -0.10880654953722058 1104 36 -0.2035751031215085 1104 37 -0.270697922301224 1104 38 -0.20416724180813617 1104 39 -0.2586122348681599 1104 40 -0.3381991429188673 1104 41 -0.26834593734737666 1104 42 -0.23448954502025288 1105 1 0.5034510774422358 1105 2 0.6968394489895005 1105 3 -2.331725960685077 1105 4 -0.9953133518667094 1105 5 -0.40259636624783823 1105 6 -1.8842829170456774 1105 7 -1.7307328725217204 1105 8 -2.0558581383211454 1105 9 1.5265176213636202 1105 10 0.3845306417342371 1105 11 0.9111835720810539 1105 12 2.9252956192021173 1105 13 0.3058472382376429 1105 14 1.788950556276884 1105 15 1.7005295138067105 1105 16 0.9060201144624145 1105 17 0.30990896362486353 1105 18 -0.011176257354208019 1105 19 0.22061084954342647 1105 20 0.049191556945949344 1105 21 -0.09742570877879331 1105 22 0.6609252620885329 1105 23 0.3337882347466987 1105 24 0.1410350096977666 1105 25 11.74978823060562 1105 26 6.708970211211202 1105 27 2.8333809965502965 1105 28 1.1726341559563522 1105 29 0.4476253206014391 1105 30 0.08977311986505754 1105 31 0.15416564977863345 1105 32 0.09869078477437654 1105 33 0.07929029297975632 1105 34 -0.030747527316954404 1105 35 -0.04458769637518145 1105 36 -0.22205179976965894 1105 37 -0.060809723815999916 1105 38 5.645257417938865E-4 1105 39 -0.27781053018129853 1105 40 -0.1955917069903137 1105 41 -0.09380956020460474 1105 42 -0.23899670855816169 1106 1 -4.8261518146277425 1106 2 -4.002909681593998 1106 3 -1.1641590329176132 1106 4 -6.999168676146272 1106 5 -0.3507889729726621 1106 6 1.1180863494947992 1106 7 -1.2355877624691907 1106 8 -2.9969258931251304 1106 9 -0.258806467617287 1106 10 0.2096552990582481 1106 11 0.8354869104197391 1106 12 3.073133538099377 1106 13 0.22448068702507484 1106 14 1.898026090941702 1106 15 1.8183869826636965 1106 16 0.8434196187420397 1106 17 0.11131186632745482 1106 18 -0.12627225678446052 1106 19 0.09938204078697055 1106 20 -0.20893311141190196 1106 21 -0.24020611628862554 1106 22 0.7940219696907533 1106 23 0.2024744955084543 1106 24 0.178231090076288 1106 25 11.892620296551879 1106 26 6.8701831512984555 1106 27 2.9107342389447197 1106 28 0.9587521799358996 1106 29 0.2642097851542218 1106 30 0.18233249948234942 1106 31 -0.0608076734035663 1106 32 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6.879500118702673 1107 27 2.8018518214320878 1107 28 1.3647251997401284 1107 29 0.5493661414669901 1107 30 0.11706836753884886 1107 31 0.14550373285068474 1107 32 0.04673784566261219 1107 33 -0.5986527844054379 1107 34 -0.2263890626683036 1107 35 -0.5557622948355103 1107 36 -0.3855110990538222 1107 37 -0.26663023499228444 1107 38 -0.17497724484650234 1107 39 -0.2616071936642186 1107 40 -0.23824316819309388 1107 41 -0.3180355901677814 1107 42 -0.16746881593690688 1113 1 -11.223250884140604 1113 2 -4.170397366323062 1113 3 0.6754687722274326 1113 4 1.3410064743737031 1113 5 2.3946954276841517 1113 6 -23.693079496153803 1113 7 -11.414843372759282 1113 8 0.881289760952594 1113 9 -21.3170987500475 1113 10 -21.923349627050126 1113 11 -10.846231754057545 1113 12 -22.261020813296355 1113 13 0.1328003367240349 1113 14 2.0389675249865817 1113 15 0.6227110111256557 1113 16 0.9698564003512331 1113 17 -0.013209206469483092 1113 18 -0.27405550801598366 1113 19 -0.5667372805047677 1113 20 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1201 4 6.489415305511283 1201 5 9.271161769499713 1201 6 9.902480872010788 1201 7 7.899841100560813 1201 8 6.743348354467603 1201 9 6.8870160306780015 1201 10 6.419909712419283 1201 11 6.072675783691356 1201 12 9.265779403271054 1201 13 0.19719906138264065 1201 14 2.010302422417851 1201 15 1.8629504384251803 1201 16 1.0291417279094333 1201 17 0.4679013030655028 1201 18 0.3314308662846841 1201 19 0.28882429081208727 1201 20 0.2448867340215557 1201 21 0.06261886150787888 1201 22 0.9381606475093918 1201 23 0.5059275721258416 1201 24 0.2529690710741832 1201 25 -4.852496046197157 1201 26 -2.8988787366608086 1201 27 -1.2176601840000458 1201 28 -0.4700583973727106 1201 29 -0.09145950681438109 1201 30 0.026533320411509465 1201 31 -0.029229371750773225 1201 32 0.16837005797045634 1201 33 0.11614379478674408 1201 34 0.14406384439017472 1201 35 0.23050152853642203 1201 36 0.13317232665966827 1201 37 0.13417800414120507 1201 38 0.08273049085750728 1201 39 0.10300976021350294 1201 40 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0.6250652540910266 2215 39 0.6631696869137613 2215 40 0.013350541964150864 2215 41 0.6844080987399934 2215 42 0.5374823102849362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 12.0 25 152.0 26 969.0 27 4083.0 28 10647.0 29 21106.0 30 36187.0 31 53673.0 32 71000.0 33 81494.0 34 70280.0 35 52442.0 36 21527.0 37 12468.0 38 620.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 97.4344281690925 1.3671140970736846 0.5474952094169175 0.6509625244168944 2 2.9497609428857343 93.10533338275081 0.8985489789110855 3.0463566954523555 3 0.6343608299363349 0.8040580772225531 97.91760179544727 0.6439792973938534 4 96.64544496862547 1.788805935968488 0.8265011679567627 0.739247927449274 5 0.5468494939601698 2.411268131150599 0.32764330021920623 96.71423907467003 6 0.6100853168043422 1.4104506544443467 97.09941893238637 0.880045096364938 7 0.6119275488774839 2.5585533593036116 0.91560117530396 95.91391791651495 8 2.9949801824924887 86.40363780406727 1.2953769959392063 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27.450877112627676 23.579214949846563 22 22.784345501513233 27.925176428220205 28.370258022664995 20.920220047601568 23 22.63500206110017 28.888150964136855 27.81614986488343 20.66069710987954 24 21.77689735721156 28.26272156826822 28.67127742408281 21.28910365043741 25 18.811289038308413 30.94081217375918 28.87728921525259 21.370609572679818 26 21.782643047715013 29.859042035474232 28.317779482657933 20.04053543415282 27 22.21275640717356 29.42158526447411 28.25751902514319 20.108139303209143 28 21.652891426372886 30.56637650491351 28.313786920048646 19.46694514866496 29 22.077410519421335 29.758919431409865 27.965529165779245 20.198140883389556 30 22.31209636788348 30.0 28.154857326066047 19.533046306050476 31 21.393532725690466 30.490770851463385 27.986305134429536 20.129391288416617 32 21.899418311729953 29.734805111528424 28.78211881097421 19.583657765767416 33 21.470251454220676 30.234965419319376 28.684560069619387 19.61022305684056 34 21.75720240003664 30.759629918014014 27.8523336234141 19.630834058535246 35 21.991480785966196 30.019465946044978 29.00448861814684 18.98456464984198 36 21.347730499702287 30.553748912197133 28.342417441487655 19.756103146612926 37 21.8616314752897 30.663216232308887 28.16104062657445 19.314111665826957 38 21.710713140658637 30.040534969999545 28.721659872669814 19.52709201667201 39 21.532313470434662 29.611368112490265 29.431365364356708 19.424953052718365 40 21.1759721522466 29.588237988366235 29.606329867631565 19.6294599917556 41 21.26574451518344 29.79091283836394 29.71556817661338 19.227774469839236 42 21.30673750744286 29.9104566481931 29.62694086932625 19.155864975037787 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 16.0 9 30.0 10 30.0 11 85.0 12 140.0 13 294.5 14 449.0 15 740.0 16 1031.0 17 1031.0 18 1494.0 19 1957.0 20 2617.0 21 3277.0 22 3277.0 23 4145.0 24 5013.0 25 6187.0 26 7361.0 27 9023.5 28 10686.0 29 10686.0 30 12938.5 31 15191.0 32 18032.5 33 20874.0 34 20874.0 35 24240.0 36 27606.0 37 29900.0 38 32194.0 39 34252.5 40 36311.0 41 36311.0 42 36583.0 43 36855.0 44 37900.0 45 38945.0 46 38742.5 47 38540.0 48 38540.0 49 37736.0 50 36932.0 51 35718.0 52 34504.0 53 34504.0 54 31879.0 55 29254.0 56 26167.0 57 23080.0 58 19464.0 59 15848.0 60 15848.0 61 12753.0 62 9658.0 63 7474.0 64 5290.0 65 3994.0 66 2698.0 67 2698.0 68 2052.5 69 1407.0 70 1046.0 71 685.0 72 685.0 73 506.0 74 327.0 75 273.0 76 219.0 77 198.0 78 177.0 79 177.0 80 133.0 81 89.0 82 59.0 83 29.0 84 29.0 85 15.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 1.28383639444877 2 1.1368112490267026 3 0.0 4 0.0 5 1.7954472587367747 6 3.71616360555123 7 0.001603077909586406 8 1.138414326936289 9 0.2858058901662621 10 0.16534603581734073 11 1.1759721522465991 12 0.5434434113497916 13 0.029084413502496218 14 0.3742041863234553 15 0.08519214033802043 16 0.0 17 0.01877891265515504 18 0.0 19 0.0 20 0.0 21 0.0 22 0.7971877433243256 23 0.0 24 0.0 25 35.287179956945906 26 0.0011450556497045756 27 2.2901112994091512E-4 28 0.001603077909586406 29 2.2901112994091512E-4 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 436660.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.44777392145812 #Duplication Level Percentage of deduplicated Percentage of total 1 86.61970022738258 65.35263559900037 2 8.725703655004494 13.166698333368402 3 2.118712138306908 4.795563432468861 4 0.807879652359004 2.4381088546771332 5 0.4172363354868305 1.5739776355814024 6 0.27641199122101445 1.25128016536939 7 0.18401306180642504 0.971836312003652 8 0.13606723096077547 0.8212775743717933 9 0.1307173063785682 0.8876096801344971 >10 0.5606199666146231 7.072709554341448 >50 0.018539282703438624 0.9570752688424728 >100 0.004084926648533268 0.584866154712287 >500 3.1422512681025127E-4 0.1263614351283538 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATGCTCAAAAACGACTCACTATAGTGAGTCGTATTACCGGC 533 0.12206293225850776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 4.5802225988183023E-4 0.0 0.0 11 0.0 0.0 6.870333898227454E-4 0.0 0.0 12 0.0 0.0 0.007786378417991114 0.0 0.0 13 0.0 0.0 0.027023313333027984 0.0 0.0 14 0.0 0.0 0.056565749095406036 0.0 0.0 15 0.0 0.0 0.0682453167223927 0.0 0.0 16 0.0 0.0 0.07488663949067925 0.0 0.0 17 0.0 0.0 0.08267301790867036 0.0 0.0 18 0.0 0.0 0.0922914853661888 0.0 0.0 19 0.0 0.0 0.11519259836028031 0.0 0.0 20 0.0 0.0 0.15595657948976321 0.0 0.0 21 0.0 0.0 0.19328539367013237 0.0 0.0 22 0.0 0.0 0.27595841157880274 0.0 0.0 23 0.0 0.0 0.3590894517473549 0.0 0.0 24 0.0 0.0 0.47886227270645354 0.0 0.0 25 0.0 0.0 0.5654284798241195 0.0 0.0 26 0.0 0.0 0.6845142673933953 0.0 0.0 27 0.0 0.0 0.7919204873356845 0.0 0.0 28 0.0 0.0 0.8482572253011497 0.0 0.0 29 0.0 0.0 0.8940594512893326 0.0 0.0 30 0.0 0.0 1.0729171437731875 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTCGC 15 0.0073748566 51.71157 22 GGACCCG 20 3.5389778E-4 51.71157 20 GACCCGG 25 0.0010627959 41.36926 21 GGCCGAT 90 0.0 40.220116 19 TCACGCG 205 0.0 34.929092 6 TCACGAT 60 1.8189894E-12 34.92909 6 TCCCGTC 30 1.2316579E-5 34.92909 6 TCCCCGC 20 0.0024418165 34.92909 6 CTCACGA 360 0.0 34.836353 5 CTCACGC 705 0.0 34.83635 5 CTCACCG 435 0.0 34.83635 5 GCGCATA 20 0.0024738736 34.83635 4 TACGCAA 25 1.7563197E-4 34.83635 3 CTCGGTC 65 0.0 34.83635 5 CTCGCTA 40 6.497066E-8 34.83635 5 GCTCGCG 80 0.0 34.83635 4 GCTCACG 2170 0.0 34.83635 4 TGCTCAC 8580 0.0 34.61304 3 CTCACGT 630 0.0 34.55987 5 TGCTCAG 9775 0.0 34.551243 3 >>END_MODULE