##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099029_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117231 Sequences flagged as poor quality 0 Sequence length 42 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.465610632000068 30.0 25.0 31.0 17.0 35.0 2 24.416033301771716 27.0 18.0 31.0 8.0 33.0 3 27.605351826735248 29.0 26.0 31.0 17.0 35.0 4 28.257329545939214 30.0 27.0 31.0 19.0 35.0 5 23.99952231065162 27.0 17.0 30.0 8.0 34.0 6 27.533655773643492 30.0 26.0 31.0 17.0 37.0 7 27.488155863210242 30.0 25.0 31.0 17.0 37.0 8 27.23823050217093 30.0 25.0 31.0 13.0 37.0 9 25.666120735982805 27.0 26.0 27.0 20.0 31.0 10 25.805827810050243 27.0 26.0 27.0 20.0 31.0 11 25.245992954082112 27.0 26.0 27.0 16.0 31.0 12 27.37055045167234 30.0 25.0 31.0 17.0 35.0 13 30.962544036986802 31.0 31.0 33.0 30.0 34.0 14 30.94179867099999 31.0 31.0 34.0 28.0 34.0 15 31.123184140713633 31.0 31.0 34.0 28.0 34.0 16 35.00008530166935 35.0 35.0 37.0 32.0 37.0 17 35.05801366532743 35.0 35.0 37.0 32.0 37.0 18 34.743489350086584 35.0 35.0 37.0 32.0 37.0 19 34.664977693613466 35.0 35.0 37.0 32.0 37.0 20 34.631206762716346 35.0 35.0 37.0 32.0 37.0 21 36.06423215702331 37.0 35.0 39.0 32.0 39.0 22 35.89660584657642 37.0 35.0 39.0 32.0 39.0 23 36.20059540565209 37.0 35.0 39.0 32.0 39.0 24 36.38066722965768 37.0 35.0 39.0 32.0 39.0 25 22.81121034538646 32.0 2.0 37.0 2.0 39.0 26 29.604422038539294 34.0 17.0 38.0 17.0 40.0 27 34.31800462335048 36.0 32.0 39.0 27.0 40.0 28 36.41381545836852 37.0 34.0 39.0 32.0 40.0 29 37.21248645835999 38.0 36.0 40.0 33.0 40.0 30 37.58547653777585 39.0 37.0 40.0 33.0 41.0 31 37.964105057535974 39.0 37.0 40.0 34.0 41.0 32 38.07190077709821 39.0 37.0 40.0 34.0 41.0 33 38.09959822913734 39.0 37.0 40.0 34.0 41.0 34 38.09527343450112 39.0 37.0 40.0 34.0 41.0 35 38.05020003241464 39.0 37.0 40.0 34.0 41.0 36 38.03386476273341 39.0 37.0 40.0 34.0 41.0 37 37.996792657232305 39.0 37.0 40.0 34.0 41.0 38 37.837713574054646 39.0 37.0 40.0 33.0 41.0 39 37.74965666078085 39.0 37.0 40.0 33.0 41.0 40 37.66462795676912 39.0 37.0 40.0 33.0 41.0 41 37.54086376470388 39.0 37.0 40.0 33.0 41.0 42 37.5209714154106 39.0 37.0 40.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.374047454690171 1101 2 6.691703782320509 1101 3 5.564102063033236 1101 4 4.678930169918285 1101 5 7.265666516051443 1101 6 8.113014218774346 1101 7 7.023900351699215 1101 8 6.749001475458716 1101 9 6.21199887150016 1101 10 5.755084725047638 1101 11 3.6120545774266297 1101 12 7.656078991876683 1101 13 0.06702491256731236 1101 14 0.940875229973269 1101 15 1.6111797247116613 1101 16 0.6010486564107964 1101 17 0.12001698220856127 1101 18 0.13654653621352253 1101 19 0.0722374063749811 1101 20 -0.10978889592023933 1101 21 -0.14872294876382597 1101 22 1.0220217836420318 1101 23 0.46221148317622607 1101 24 0.03386590084810592 1101 25 12.067275664743448 1101 26 6.943787808628191 1101 27 2.9730481161044935 1101 28 1.511839146151452 1101 29 0.6277154327620522 1101 30 0.2041022438107376 1101 31 -0.07508669975204896 1101 32 -0.2708734675050408 1101 33 -0.04367605208740599 1101 34 -0.23555479525379752 1101 35 -0.4166917085033077 1101 36 -0.4433985647208658 1101 37 -0.43474523603317294 1101 38 -0.6347096475972549 1101 39 -0.52079220870678 1101 40 -0.6850363136289488 1101 41 -0.2538193911072426 1101 42 -0.2516879847158364 1102 1 4.241352146996949 1102 2 3.1463823944361167 1102 3 2.2344179929006955 1102 4 1.2468417937080964 1102 5 4.081963559195575 1102 6 4.760001591900263 1102 7 2.934531774792781 1102 8 3.268918275433311 1102 9 4.129378388257262 1102 10 3.371276974266646 1102 11 3.951908421656814 1102 12 5.604722029630281 1102 13 -0.15792477444022524 1102 14 1.2745644921684622 1102 15 1.6581861512785103 1102 16 1.032593215661045 1102 17 0.3469236163785254 1102 18 0.2593380240887839 1102 19 0.4875746324321497 1102 20 0.346318724265295 1102 21 0.3419587277801952 1102 22 0.9878645213039405 1102 23 0.6676869657641618 1102 24 0.24791146238873552 1102 25 12.112837205372927 1102 26 7.16023092815281 1102 27 3.0499446281339644 1102 28 1.6324831524549666 1102 29 0.6814977392604717 1102 30 0.40985558907893704 1102 31 0.10781045533104816 1102 32 0.06358190179430778 1102 33 0.15119301504818594 1102 34 0.1945210612579089 1102 35 0.16644282069807304 1102 36 0.04090417857768358 1102 37 -0.25741327348906395 1102 38 -0.17722859187784934 1102 39 0.10024279008253245 1102 40 -0.11683491875829333 1102 41 0.10199964883749857 1102 42 0.05003516084848059 1103 1 1.3806185193630327 1103 2 -0.5506048171417284 1103 3 0.39466010822404485 1103 4 0.47262798585300203 1103 5 1.1262542796251935 1103 6 1.9254865052029544 1103 7 -2.232295695284133 1103 8 -1.172792005365853 1103 9 1.9928517419185745 1103 10 1.349131614051835 1103 11 -1.6096398421214602 1103 12 1.7441067290340975 1103 13 -0.513620400890872 1103 14 1.2157514033776735 1103 15 1.5807820644446693 1103 16 0.8898452977618092 1103 17 0.21461578403883408 1103 18 0.021168452341640887 1103 19 -0.05876405595815015 1103 20 0.3024239443080745 1103 21 -0.10124429411030178 1103 22 0.816417745715718 1103 23 0.19594892362152905 1103 24 0.47730662918434774 1103 25 11.942887721915952 1103 26 7.195861019628712 1103 27 2.7904215771168097 1103 28 1.148416343189048 1103 29 0.7493673974773714 1103 30 0.2320328065731374 1103 31 0.07349106334998368 1103 32 -0.1887186488815189 1103 33 0.30394411867536775 1103 34 0.3337692294643446 1103 35 0.36368944155093885 1103 36 0.3503794436757559 1103 37 0.14163100812928775 1103 38 0.05783717064888805 1103 39 -0.3213063839037247 1103 40 -0.09370653392235795 1103 41 0.34180304391327354 1103 42 -0.10315739365022125 1104 1 1.3802670339761605 1104 2 1.4766810415900338 1104 3 0.052563714560413644 1104 4 -0.10552214174100882 1104 5 1.5181075224390028 1104 6 1.3791626571601974 1104 7 -0.3316697624033971 1104 8 0.00955187252908729 1104 9 2.114942357740233 1104 10 1.0848820112784736 1104 11 0.8305209744802049 1104 12 2.9676081012715656 1104 13 0.2855081079444126 1104 14 1.8314719484207664 1104 15 1.7441216571068097 1104 16 0.8155422499501626 1104 17 -0.09432542641906139 1104 18 -0.06145950278378365 1104 19 0.0657532534934333 1104 20 0.028385714253666094 1104 21 -0.16636538804838352 1104 22 0.6234185642433303 1104 23 0.08701252803194848 1104 24 0.050685222279824416 1104 25 11.677487365615697 1104 26 6.922390944035275 1104 27 2.6315549971177674 1104 28 0.9461052063986415 1104 29 0.15902120844564394 1104 30 0.10525250901427086 1104 31 -0.3500584575706185 1104 32 -0.20290325093264983 1104 33 -0.012594953981000856 1104 34 -0.05585472932038016 1104 35 -0.2034201930005537 1104 36 0.14289232344849268 1104 37 -0.051281505713461684 1104 38 -0.01931146433594222 1104 39 -0.3406091909719464 1104 40 -0.4153686264641294 1104 41 -0.268799296112725 1104 42 -0.0391052004777066 1105 1 0.5731755747107883 1105 2 0.7362765185768154 1105 3 -1.290600367734104 1105 4 -1.0471746920892109 1105 5 -1.0384229036566381 1105 6 -2.3142164275232346 1105 7 -1.8146505497320113 1105 8 -5.568449835907405 1105 9 1.7079869942631305 1105 10 0.5911438653664121 1105 11 0.8347196743310796 1105 12 3.2276379265187813 1105 13 0.30099986256660216 1105 14 1.9189931228984634 1105 15 1.683790683732358 1105 16 0.8086071560585495 1105 17 0.21704436321159903 1105 18 0.07855989810518338 1105 19 -0.008983603852115607 1105 20 -0.2041353878326504 1105 21 -0.3951945299210067 1105 22 0.9330654391075939 1105 23 0.4523862847100588 1105 24 0.15039710460040823 1105 25 11.934143752706866 1105 26 6.931801823011373 1105 27 2.9711284671260216 1105 28 1.2153432147670813 1105 29 0.6490963506946983 1105 30 0.46246051839619895 1105 31 0.10395130078696724 1105 32 0.027750153633796515 1105 33 0.21804397231009887 1105 34 0.3972863325163374 1105 35 -0.003952993533353322 1105 36 0.08980048774796501 1105 37 0.03463258020062199 1105 38 0.15531101463436414 1105 39 -0.07328231494941662 1105 40 -0.23702523072942938 1105 41 0.05688950783694935 1105 42 -0.008946952928148733 1106 1 -3.5456859559154488 1106 2 -4.888804282974572 1106 3 -1.3008646132924682 1106 4 -5.910174437441896 1106 5 -0.2944609832241305 1106 6 0.8168681741496009 1106 7 -1.2283936693574837 1106 8 -3.7549104341327073 1106 9 -0.41921913294299884 1106 10 0.957248349117954 1106 11 1.2055057509995066 1106 12 2.950679590736918 1106 13 0.1459392369177408 1106 14 1.63429742643806 1106 15 1.6001684236395093 1106 16 0.7410080884594805 1106 17 -0.16898137575531536 1106 18 -0.5213304076674774 1106 19 -0.07284131476865241 1106 20 -0.07830043846828971 1106 21 -0.32152310330840095 1106 22 0.3852015383025176 1106 23 0.24874678695291408 1106 24 -0.034809845312423704 1106 25 11.413938467795703 1106 26 6.586500065944914 1106 27 2.3511993766086974 1106 28 0.8737632261282684 1106 29 0.18861565491988586 1106 30 -0.3301390389092447 1106 31 -0.3496930940339027 1106 32 -0.23590775708294132 1106 33 -0.11660177410035288 1106 34 -0.3772528537875601 1106 35 -0.5255490445411723 1106 36 -0.4858983232449674 1106 37 -0.6507212816238237 1106 38 -0.32987047054712093 1106 39 -0.4043042342066343 1106 40 -0.12819288660296735 1106 41 -0.3238474023117277 1106 42 -0.3205569115969311 1107 1 2.8932998948351063 1107 2 1.727729510029807 1107 3 0.7218316446979074 1107 4 -4.155954800869321 1107 5 -20.227234092467828 1107 6 -23.241955355262164 1107 7 -9.308046043127504 1107 8 -5.580881848339416 1107 9 1.3964096826858174 1107 10 2.0472433214658707 1107 11 2.204572044183447 1107 12 3.779308478189332 1107 13 0.03992760149434105 1107 14 1.7301819340872733 1107 15 1.4670074669491413 1107 16 0.7666491141005096 1107 17 0.07873822490545734 1107 18 -0.18095836141307586 1107 19 -0.08979168466019871 1107 20 -0.14430632800359433 1107 21 -0.056422191148669754 1107 22 0.2959248019669616 1107 23 0.45782529014906004 1107 24 0.08046703467033467 1107 25 12.335076153639271 1107 26 6.502897394106949 1107 27 2.3184478144453706 1107 28 0.8897798892037585 1107 29 0.20154390314225168 1107 30 -0.2881222321865522 1107 31 -0.5067713099356439 1107 32 -0.07947595359231485 1107 33 -0.6995939453278126 1107 34 -0.4962645610345575 1107 35 -1.447620437200797 1107 36 -0.43389803595056264 1107 37 -0.7252741797061333 1107 38 -0.972117112793768 1107 39 -1.1937174353845421 1107 40 -0.6185326122368124 1107 41 -1.122597671650233 1107 42 -0.7929407369219348 1113 1 -10.226358224823013 1113 2 -4.422697840397543 1113 3 -0.4285957057294425 1113 4 1.4679768230623047 1113 5 2.5727659075321725 1113 6 -23.241955355262164 1113 7 -10.402063137144598 1113 8 0.7644172969597278 1113 9 -20.80017151389538 1113 10 -21.474124199901652 1113 11 -12.639872400260996 1113 12 -21.690776991896136 1113 13 1.2142865758533183 1113 14 2.7250537289590717 1113 15 2.3900843900260647 1113 16 0.1410080884594791 1113 17 0.9727553189225517 1113 18 -1.1997617802164982 1113 19 -0.8145780094465209 1113 20 -0.9836225673198342 1113 21 0.6034068686803948 1113 22 1.9745572805994414 1113 23 -1.0156789833552153 1113 24 1.721492675695977 1113 25 11.894050512613632 1113 26 8.877256368465918 1113 27 2.867165763163321 1113 28 1.4367884362123036 1113 29 1.7656464672448138 1113 30 1.578544434480115 1113 31 1.006049202884867 1113 32 1.7461650720487114 1113 33 -2.044893090626964 1113 34 -0.31506797983797696 1113 35 1.082294092713731 1113 36 0.5387515366990101 1113 37 1.243956589524636 1113 38 1.8415581008814499 1113 39 1.6763680347009284 1113 40 0.5900143963102025 1113 41 1.3697100206574575 1113 42 0.48056353658233775 1114 1 -8.916013397236807 1114 2 -2.0709737024665067 1114 3 -0.8354922574535806 1114 4 -7.256161107972179 1114 5 -1.0893030579850702 1114 6 -0.9316105276759572 1114 7 -0.8158562405928755 1114 8 0.6333828142011093 1114 9 -5.144999100102275 1114 10 1.42242752423628 1114 11 1.7946103583596944 1114 12 -21.690776991896136 1114 13 -1.2270927344915137 1114 14 -27.47494627104093 1114 15 -14.23060526514635 1114 16 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1201 5 7.721382508322687 1201 6 8.947767964895938 1201 7 8.259992198823785 1201 8 7.301571447157354 1201 9 7.122753387290391 1201 10 6.500184100493605 1201 11 6.560522856656 1201 12 9.662977948815325 1201 13 0.30875297901537024 1201 14 1.9954094601843657 1201 15 1.9276337971406896 1201 16 0.9643282465622462 1201 17 0.4257197458000235 1201 18 -0.03454438891214551 1201 19 0.27119274154161843 1201 20 0.21716794651415938 1201 21 0.20894046551833867 1201 22 1.1263359367259227 1201 23 0.6270087636803581 1201 24 0.12465473103194569 1201 25 -5.161680712682813 1201 26 -3.449621101889811 1201 27 -1.6004231696430011 1201 28 -0.8458202594398685 1201 29 -0.240282386510124 1201 30 -0.10959785801000521 1201 31 0.10881600130383617 1201 32 0.003477325013143684 1201 33 -0.06623696414474978 1201 34 0.21339051818574006 1201 35 0.1732031836228245 1201 36 0.0755104299796443 1201 37 -0.08252562391410123 1201 38 0.10519446451781533 1201 39 0.1439569675072505 1201 40 -0.12974844953960485 1201 41 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2215 37 -0.4560434104753668 2215 38 -0.7584418991185515 2215 39 0.6097013680342656 2215 40 -0.27665227035646467 2215 41 -0.34695664600920395 2215 42 0.5638968699156734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 5.0 25 57.0 26 431.0 27 1465.0 28 3542.0 29 6653.0 30 10918.0 31 15069.0 32 19170.0 33 21156.0 34 17789.0 35 12715.0 36 5334.0 37 2830.0 38 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 95.47340723806236 2.219729974550317 1.2026053573739377 1.1042574300133718 2 3.3043059236567656 90.58617010291536 2.565139572505421 3.544384400922452 3 2.1146283832774606 1.8459281248134025 1.0577406999854988 94.98170279192364 4 0.8751951275686466 1.0372682993406181 96.43012513754894 1.6574114355417935 5 2.3942537909018355 91.20111731843575 0.9906658801485131 5.413963010513897 6 1.154844519785983 2.663735409934488 1.86646889512811 94.31495117515142 7 92.90101340976558 4.674651107244003 0.9613744156686116 1.4629610673218 8 1.8311046861823876 2.985010411826458 93.97490835871753 1.208976543273616 9 36.00085561497326 24.610909090909093 20.861604278074868 18.52663101604278 10 32.32798037724239 20.332971531617765 26.517216918645893 20.821831172493955 11 30.928971157985362 20.504520017219114 26.037882049074472 22.52862677572105 12 31.32086799897075 23.682133973754183 22.685479029076248 22.311518998198817 13 31.448220547956897 27.725492538332237 21.280045051578938 19.54624186213193 14 28.397661419412273 30.603562654614247 22.24989942049083 18.748876505482652 15 22.245845927899314 26.262445138924466 31.97312020766091 19.51858872551531 16 21.212819134870468 32.303742184234544 28.83196424154021 17.65147443935478 17 20.178492201631343 31.57827377905191 27.64154807003174 20.601685949285006 18 21.496873693818188 30.336685688938932 25.64594689118066 22.52049372606222 19 23.50828705717771 28.727043188235196 26.591089387619316 21.17358036696778 20 21.34418370567512 28.670744086461774 28.001978998729005 21.983093209134104 21 22.392541222031713 27.051718402129133 28.762869889363735 21.792870486475422 22 22.338247690481516 28.450515233360285 28.677596380464138 20.53364069569406 23 21.81078383703969 29.123695950729754 28.92920814460339 20.136312067627163 24 22.504286408885022 28.249353839854646 29.479403911934554 19.766955839325774 25 18.552388870125483 31.061806656101425 29.257488763607075 21.128315710166014 26 20.937334720966337 30.132393837544573 30.130687731390648 18.799583710098442 27 21.203435951241566 30.94318055804352 29.726778753060195 18.126604737654716 28 21.019722250656837 30.91411608148224 29.259221346436004 18.806940321424914 29 20.422925676655492 30.41772227482492 29.505847429434194 19.653504619085396 30 20.28388395560901 30.78366643635216 29.658537417577264 19.273912190461566 31 19.352389726266946 31.617063745937507 30.088457831119754 18.942088696675793 32 20.422072659961955 31.454137557472002 29.894823041686923 18.22896674087912 33 20.584145831733927 29.980977727734132 29.890557958219244 19.544318482312697 34 20.997858928099223 30.631829464902626 28.499287731060896 19.87102387593725 35 19.601470600779656 30.9218551407051 29.79843215531728 19.67824210319796 36 19.132311419334478 31.078810212315854 29.864114440719604 19.924763927630064 37 20.225878820448518 30.033011746039872 30.547380812242498 19.19372862126912 38 19.759278689083946 31.967653606981088 29.993772978137184 18.279294725797783 39 19.638150318601735 31.48143409166517 30.538850645307132 18.34156494442596 40 19.65521065247247 32.56561830915031 29.38386604225845 18.395304996118774 41 19.25002772304254 32.74560483148655 29.341215207581612 18.663152237889296 42 20.08513106601496 31.541145260212744 28.696334587267874 19.677389086504423 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 10.5 9 21.0 10 21.0 11 51.5 12 82.0 13 156.5 14 231.0 15 395.0 16 559.0 17 559.0 18 818.0 19 1077.0 20 1232.0 21 1387.0 22 1387.0 23 1751.5 24 2116.0 25 2638.5 26 3161.0 27 3553.5 28 3946.0 29 3946.0 30 4641.5 31 5337.0 32 6076.5 33 6816.0 34 6816.0 35 7618.5 36 8421.0 37 8715.0 38 9009.0 39 8853.0 40 8697.0 41 8697.0 42 8580.0 43 8463.0 44 8670.0 45 8877.0 46 9034.5 47 9192.0 48 9192.0 49 9377.0 50 9562.0 51 9099.0 52 8636.0 53 8636.0 54 7942.5 55 7249.0 56 6463.5 57 5678.0 58 4579.5 59 3481.0 60 3481.0 61 2813.0 62 2145.0 63 1675.0 64 1205.0 65 1024.0 66 843.0 67 843.0 68 644.5 69 446.0 70 390.5 71 335.0 72 335.0 73 232.5 74 130.0 75 105.0 76 80.0 77 58.5 78 37.0 79 37.0 80 24.0 81 11.0 82 6.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 1.1225699686942874 2 0.8692240107138897 3 0.0 4 0.0 5 1.6676476358642338 6 3.3856232566471327 7 0.002559050080610078 8 0.8675179773268162 9 0.3036739428990625 10 0.19107573935221914 11 0.9221110457131646 12 0.54678370055702 13 0.02729653419317416 14 0.34803081096297056 15 0.09980295314379302 16 0.0 17 0.023031450725490697 18 0.0 19 0.0 20 0.0 21 0.0 22 0.8299852428112018 23 0.0 24 0.0 25 34.33306889815834 26 0.0042650834676834625 27 0.0 28 0.002559050080610078 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 117231.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.50962629338656 #Duplication Level Percentage of deduplicated Percentage of total 1 88.19640328676708 67.47873855891359 2 7.466580446634631 11.42530559323046 3 1.9812025464640497 4.547431993244108 4 0.8027382292932558 2.4566880773856745 5 0.42478231300101454 1.6249968011873992 6 0.25308552506884596 1.1618087365969751 7 0.1516283321998372 0.8120718922469313 8 0.10591684969841571 0.6482926870878863 9 0.08250365134402908 0.5681091178954373 >10 0.49725173647887794 6.7874538304714624 >50 0.030102683598497094 1.5704037328010507 >100 0.00780439945146221 0.9186989789390178 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GACTACGTTGCGGACTACGTTTTTTTTTTTTTTTTTTTTTTT 236 0.2013119396746594 No Hit GACTACGTGTTTGCCACACCCATGTCATGTTGCCTCGATATG 187 0.15951412169136148 No Hit GACTACGTTGCGGACTACGTTTTTNTTTTTTTTTTTTTTTTT 144 0.12283440386928372 No Hit GACTACGTCAAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.11601027032099019 No Hit GACTACGTGTTTGCCACACCCATGNCATGTTGCCTCGATATG 134 0.1143042369339168 No Hit GACTACGTGTGCGGGGTTGGGACGTGAGAATACTCATGGGTG 121 0.1032150199179398 No Hit GACTACGTCTTTAGAGAAGTGGGGAGGGGTCTCGGATTTTAT 119 0.10150898653086642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 8.530166935366925E-4 0.0 0.0 12 0.0 0.0 0.01023620032244031 0.0 0.0 13 8.530166935366925E-4 0.0 0.015354300483660465 0.0 0.0 14 8.530166935366925E-4 0.0 0.02047240064488062 0.0 0.0 15 8.530166935366925E-4 0.0 0.029002567580247544 0.0 0.0 16 8.530166935366925E-4 0.0 0.04179781798329793 0.0 0.0 17 8.530166935366925E-4 0.0 0.04776893483805478 0.0 0.0 18 8.530166935366925E-4 0.0 0.06141720193464186 0.0 0.0 19 8.530166935366925E-4 0.0 0.08786071943427932 0.0 0.0 20 8.530166935366925E-4 0.0 0.1364826709658708 0.0 0.0 21 8.530166935366925E-4 0.0 0.18681065588453566 0.0 0.0 22 8.530166935366925E-4 0.0 0.31476315991503956 0.0 0.0 23 8.530166935366925E-4 0.0 0.3975057791880987 0.0 0.0 24 8.530166935366925E-4 0.0 0.47257124821932767 0.0 0.0 25 8.530166935366925E-4 0.0 0.5109569994284788 0.0 0.0 26 8.530166935366925E-4 0.0 0.6278202864430057 0.0 0.0 27 8.530166935366925E-4 0.0 0.8453395432948623 0.0 0.0 28 8.530166935366925E-4 0.0 0.9059037285359675 0.0 0.0 29 8.530166935366925E-4 0.0 0.9417304296645085 0.0 0.0 30 8.530166935366925E-4 0.0 1.0355622659535446 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGCGG 35 4.343201E-8 51.15855 19 GAAGCGG 15 0.008060303 50.526966 24 CGAAGCG 15 0.008060303 50.526966 23 CGGCAAG 35 4.7908543E-8 50.526962 23 CGGCGGC 45 3.133191E-7 39.789986 20 GGCGGCA 45 3.133191E-7 39.789986 21 GCGGCAA 50 7.1541035E-7 35.810986 22 GCAAGAT 50 7.8863013E-7 35.368877 25 GGCAAGA 50 7.8863013E-7 35.368877 24 GGATACG 20 0.002379638 35.090706 1 TGCTACG 20 0.002379638 35.090706 1 AACTACC 20 0.002379638 35.090706 1 GATTACG 20 0.002379638 35.090706 1 GCCCACG 30 1.1854643E-5 35.090702 1 CACTACG 65 0.0 35.090702 1 GAGCACG 125 0.0 35.090702 1 GGCTCCG 45 4.321919E-9 35.090702 1 GACTAAG 155 0.0 35.090702 1 GGCTACG 170 0.0 35.090702 1 CGTGGTA 25 1.747298E-4 34.839157 6 >>END_MODULE