FastQCFastQC Report
Thu 26 May 2016
SRR2099028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352213
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG351.0804263E-437.82671725
CCGGGGT505.079819E-737.467121
TCCGGGG505.079819E-737.467120
ACGCATG200.002231473835.57454
CGTCTCG552.0008883E-1135.57455
CGTACCT5100.035.57455
ACGTCGA601.8189894E-1235.57454
CGTGTCG453.8289727E-935.57455
CGCGTTG200.002231473835.57453
CGCGTTC200.002231473835.57453
CCCGTCG502.728484E-1035.5744973
GCGTATC502.728484E-1035.5744974
TGCCCGC502.8012437E-1035.4850651
GCGCGTT850.035.4850652
GCCCGCA502.8012437E-1035.4850652
GCACGGT200.00225921335.485062
GCCCGTA10700.035.485062
ACGTACT18600.035.478874
CACGTAC61300.035.4584353
ACGTAAC15900.035.3507584
Produced by FastQC (version 0.11.5)