FastQCFastQC Report
Thu 26 May 2016
SRR2099027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254507
Sequences flagged as poor quality0
Sequence length43
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGATCACCAAGATTTGTGCCAAAGCTTAAAGAGTGAGTTAG3340.1312341114389781No Hit
ATAGATAGCATGTCGACTCACTATAGTGAGTCGTATTACCGGC2700.10608745535486254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTAC203.063386E-453.232322
GTATTGT651.8189894E-1244.47031424
TTGTGTC259.204008E-442.58584223
ATTGTGA703.6379788E-1241.2938626
TTGGTAT801.4551915E-1136.59720621
TGGTATT801.4551915E-1136.59720622
CTCGTCT405.4691554E-835.5135965
ATCAGCG200.002249107235.5135965
ATTGTTC200.002249107235.5135965
ATCGTGT4050.035.5135965
CTCGCGT200.002249107235.5135965
CTCGCAT251.5664879E-435.5135965
ATCGGCC1700.035.5135965
TTCGCTA200.002249107235.5135965
ATCGCTG2900.035.5135965
AAATCGC601.8189894E-1235.5063363
AAATCGA502.7830538E-1035.5063363
ATATCGT357.7419463E-735.5063363
GTTCGTT251.568373E-435.5063364
GTTCGCC251.568373E-435.5063364
Produced by FastQC (version 0.11.5)