##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099027_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254507
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.237478733394365	30.0	27.0	31.0	19.0	35.0
2	28.93358139461783	30.0	27.0	31.0	23.0	35.0
3	23.271104527576846	25.0	17.0	29.0	8.0	32.0
4	26.79504296541942	29.0	25.0	31.0	13.0	35.0
5	21.379773444345343	24.0	16.0	28.0	8.0	32.0
6	26.61522865775794	28.0	24.0	31.0	17.0	34.0
7	28.438655911232303	30.0	27.0	31.0	17.0	37.0
8	27.841768595755717	30.0	26.0	31.0	16.0	37.0
9	25.686507640261368	26.0	26.0	27.0	20.0	31.0
10	25.862357420424587	27.0	26.0	27.0	20.0	31.0
11	25.46306388429395	27.0	26.0	27.0	18.0	31.0
12	27.73971246370434	30.0	26.0	31.0	18.0	35.0
13	27.353550197047625	30.0	25.0	31.0	17.0	35.0
14	31.062450148718895	31.0	31.0	33.0	30.0	34.0
15	30.981914053444502	31.0	31.0	34.0	28.0	34.0
16	31.267752949820633	31.0	31.0	34.0	30.0	34.0
17	35.17634878411989	35.0	35.0	37.0	33.0	37.0
18	35.219102028627894	35.0	35.0	37.0	33.0	37.0
19	35.08730211742702	35.0	35.0	37.0	32.0	37.0
20	35.01092700790155	35.0	35.0	37.0	32.0	37.0
21	34.99088040800449	35.0	35.0	37.0	32.0	37.0
22	36.51025708526681	37.0	35.0	39.0	32.0	39.0
23	36.31650602930371	37.0	35.0	39.0	32.0	39.0
24	36.52245321346761	37.0	35.0	39.0	33.0	39.0
25	36.715819211259415	38.0	35.0	39.0	33.0	39.0
26	22.80406039912458	32.0	2.0	37.0	2.0	39.0
27	29.737260664736137	34.0	17.0	38.0	17.0	40.0
28	34.767263768776495	36.0	32.0	39.0	27.0	40.0
29	36.727767802064385	37.0	36.0	39.0	32.0	40.0
30	37.45126460175948	38.0	37.0	40.0	33.0	41.0
31	37.83814197644858	39.0	37.0	40.0	34.0	41.0
32	38.21300789369251	39.0	38.0	40.0	34.0	41.0
33	38.336544770870745	40.0	38.0	40.0	34.0	41.0
34	38.36771483692001	40.0	38.0	40.0	34.0	41.0
35	38.39636237903083	40.0	38.0	40.0	34.0	41.0
36	38.389018769621266	40.0	38.0	41.0	34.0	41.0
37	38.43531219180612	40.0	38.0	41.0	34.0	41.0
38	38.41747378264645	40.0	38.0	41.0	34.0	41.0
39	38.33544853383208	40.0	38.0	41.0	34.0	41.0
40	38.27003579469327	40.0	38.0	41.0	34.0	41.0
41	38.26826767043735	40.0	38.0	41.0	34.0	41.0
42	38.21559328427116	40.0	38.0	41.0	34.0	41.0
43	38.175818346843116	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.010055014539738
1101	2	5.0134818850181055
1101	3	3.8784141247585175
1101	4	7.205239600817276
1101	5	6.479836476972611
1101	6	5.259784666462604
1101	7	5.883421846014372
1101	8	5.723491699045102
1101	9	5.753364996961086
1101	10	5.473720410098316
1101	11	3.401817882530281
1101	12	7.685219166218282
1101	13	6.437996241901914
1101	14	0.0032979025611439283
1101	15	0.7707139753922405
1101	16	1.5157544186107614
1101	17	0.8114511760287897
1101	18	0.48438776937913275
1101	19	0.17236921538192718
1101	20	0.27014062058871247
1101	21	0.07903869116195494
1101	22	0.10676920587928862
1101	23	1.0539551191695509
1101	24	0.5170726079733328
1101	25	0.1706820202265078
1101	26	12.557547476312767
1101	27	6.891360836565578
1101	28	2.7985748700160045
1101	29	1.3187565959803678
1101	30	0.6741458320797946
1101	31	0.3004767195717051
1101	32	-0.021022229683566707
1101	33	-0.11878712298569383
1101	34	0.017114101943484172
1101	35	-0.13910184758790223
1101	36	-0.10324372471385601
1101	37	0.017765131090200725
1101	38	-0.20227400357878622
1101	39	-0.14351186531516902
1101	40	-0.2929804938556586
1101	41	-0.2785795502266808
1101	42	-0.24655169507659735
1101	43	-0.28350309519676387
1102	1	3.2281735003622245
1102	2	1.811273915195006
1102	3	-0.4007049584797713
1102	4	4.026580182137415
1102	5	3.2003520320015184
1102	6	2.423079143081921
1102	7	2.9124360111874985
1102	8	1.7698802958091804
1102	9	4.247383432916632
1102	10	3.228888550555215
1102	11	3.7398861064278996
1102	12	5.433887323955805
1102	13	3.884255974595373
1102	14	-0.11802441067235847
1102	15	1.2471703615697152
1102	16	1.5463337144500784
1102	17	0.9967463537476462
1102	18	0.4970692776318657
1102	19	0.16443247233490865
1102	20	0.279617807698358
1102	21	0.28916612340956505
1102	22	0.4153443444945708
1102	23	1.210517558995953
1102	24	0.8167013214556746
1102	25	0.3857023373012396
1102	26	12.656632097052476
1102	27	7.210030327379695
1102	28	2.814105212320449
1102	29	1.3615656352271301
1102	30	0.8514104576580763
1102	31	0.4080090960522824
1102	32	0.10626930429260995
1102	33	0.1386299670562252
1102	34	0.10673141021497656
1102	35	0.20503187480630203
1102	36	-0.059434878022599946
1102	37	0.014711398228875794
1102	38	-0.1499359353548897
1102	39	-0.13561215217350764
1102	40	-0.1502302087253895
1102	41	-0.0701036517577478
1102	42	-0.1386983927496388
1102	43	-0.11376788370663604
1103	1	-0.4449789203621677
1103	2	-0.28580724529563284
1103	3	-1.5393295703202483
1103	4	1.8352419311994765
1103	5	1.0262213942440432
1103	6	-2.2804175199720973
1103	7	-0.6180238158657332
1103	8	-0.9657208998672502
1103	9	2.578514151733522
1103	10	1.4490369193095134
1103	11	-0.8998408134043352
1103	12	2.057250248979848
1103	13	1.3747645173572174
1103	14	-0.3198343373486132
1103	15	1.4119011092077365
1103	16	1.7356424777735953
1103	17	1.1629852068269528
1103	18	0.6284786006656446
1103	19	0.18821890779209838
1103	20	0.37015216938547013
1103	21	0.2904950975476126
1103	22	0.3129843145926543
1103	23	0.9992426945202908
1103	24	0.7674742380035084
1103	25	0.40261073115393486
1103	26	12.566777233437445
1103	27	7.423238609050156
1103	28	2.9268438497416795
1103	29	1.5071803708283298
1103	30	0.618822971030383
1103	31	0.45251972234135707
1103	32	0.2639969186339215
1103	33	0.1086519932013843
1103	34	0.16172772251189116
1103	35	0.16122976786191145
1103	36	0.08423243011426251
1103	37	0.0012225108744416957
1103	38	-0.05884516582082
1103	39	-0.0970588926342657
1103	40	-0.30178129567212153
1103	41	-0.1386669086250265
1103	42	-0.03934256492784982
1103	43	0.06784223651148835
1104	1	0.7981195751828949
1104	2	-0.5287109893922306
1104	3	-1.5378202465946558
1104	4	1.6020270716639615
1104	5	-0.05250687879748739
1104	6	-0.2258384265400899
1104	7	-0.06570870802211815
1104	8	-0.29777211949819815
1104	9	2.298510256704553
1104	10	1.1673776369685989
1104	11	0.7751677069715335
1104	12	2.714098196786459
1104	13	0.846209086226196
1104	14	0.31422769098772463
1104	15	1.9201050139742755
1104	16	1.709399720093085
1104	17	0.9215420985938394
1104	18	0.4031511747929173
1104	19	0.20024480973474823
1104	20	0.35530237144010357
1104	21	0.0858600130741678
1104	22	0.03468449473773916
1104	23	1.0694019038294158
1104	24	0.5889358476242279
1104	25	0.3096656023745439
1104	26	12.586690569042876
1104	27	7.359895700437274
1104	28	2.9994861400187176
1104	29	1.3172003328517974
1104	30	0.6549493647204372
1104	31	0.26555833181600974
1104	32	0.08243988080344877
1104	33	0.0020255751674014277
1104	34	0.014495627473188222
1104	35	0.016578129515352202
1104	36	0.1645187147435081
1104	37	-0.06246121277325045
1104	38	0.01282336721389754
1104	39	-0.12364246534958312
1104	40	-0.01783368381175876
1104	41	-0.09879154955204683
1104	42	-0.17732396616452206
1104	43	-0.049787638360712094
1105	1	1.1747592985719848
1105	2	-1.6343640226593408
1105	3	-1.9415508542590807
1105	4	-1.2468782629028006
1105	5	-0.27343164487625415
1105	6	-1.6730927902895019
1105	7	-3.167011387690305
1105	8	-1.2071395843531434
1105	9	1.7880418076364464
1105	10	0.8110388897046299
1105	11	1.1273016853259215
1105	12	2.9027749431708543
1105	13	0.14522082904679934
1105	14	0.24913010375064815
1105	15	1.8078905596388353
1105	16	1.7534653747439606
1105	17	0.8819248650062335
1105	18	0.39965208722473733
1105	19	0.11615751958142795
1105	20	0.0865853870651776
1105	21	-0.020867718281635916
1105	22	-0.03419930542705174
1105	23	1.0356717511171425
1105	24	0.40428391129730556
1105	25	0.11587418277216699
1105	26	12.393422849920231
1105	27	7.149314524335406
1105	28	2.6981703172882945
1105	29	1.3350021051431895
1105	30	0.5588483046819164
1105	31	0.22578843650556024
1105	32	0.04434926444438503
1105	33	-0.2013339937401568
1105	34	-0.20912144435364866
1105	35	-0.04410743283078489
1105	36	-0.17271810807284993
1105	37	-0.1356599813694217
1105	38	-0.3423911427268109
1105	39	-0.21195373350344227
1105	40	-0.1928355794457346
1105	41	-0.3290731649354939
1105	42	-0.20047193177013867
1105	43	-0.3319105462126757
1106	1	-3.2840169778681734
1106	2	-1.2067622094121582
1106	3	-4.645696146874393
1106	4	0.6168589763337735
1106	5	0.4118841089819867
1106	6	-1.037450695596089
1106	7	-3.0588932501250916
1106	8	-3.083531717246988
1106	9	-1.0159615976592242
1106	10	0.12819706859907498
1106	11	0.6036923629652087
1106	12	2.90442229133323
1106	13	-3.9619615798360748
1106	14	0.191995354454626
1106	15	1.8691756949411271
1106	16	1.824873260768424
1106	17	0.8428163539499351
1106	18	0.24374484515041672
1106	19	0.045798872095012655
1106	20	0.1862659586891695
1106	21	-0.04606399574047515
1106	22	-0.03155219738167858
1106	23	0.7806718602612293
1106	24	0.5661256814689537
1106	25	-0.01285472513204411
1106	26	12.42538606108494
1106	27	7.1677584206339695
1106	28	2.728535658945688
1106	29	1.2711993794513958
1106	30	0.5671650844946257
1106	31	0.38298794462951946
1106	32	-0.1589046847526845
1106	33	-0.01745384850575249
1106	34	-0.017038029162243618
1106	35	0.033246623327485736
1106	36	-0.09894252287860894
1106	37	-0.10014885958644726
1106	38	-0.10925499638641156
1106	39	-0.140740851590067
1106	40	-0.026591664228867273
1106	41	-0.06352558902577954
1106	42	-0.013586685868496318
1106	43	0.00852006361174773
1107	1	1.1119040386658945
1107	2	0.39236525212990614
1107	3	-0.8754333816501365
1107	4	-22.580211596236133
1107	5	-18.027530005531993
1107	6	-8.98970865650583
1107	7	-6.209024733105906
1107	8	-1.078265706768498
1107	9	0.8205980313781076
1107	10	1.3040229356172546
1107	11	1.879565737828873
1107	12	3.885793862906141
1107	13	2.505997389444417
1107	14	0.26168467991013245
1107	15	1.8241302279256253
1107	16	1.8074291828555573
1107	17	1.0319042702290702
1107	18	0.6849254485669718
1107	19	0.10546471127761237
1107	20	-0.07851333056669318
1107	21	0.46557542640353944
1107	22	-0.10751122019213
1107	23	1.0017974616369543
1107	24	0.4777276326362454
1107	25	0.3063511578112994
1107	26	12.489976657208036
1107	27	7.203168493635463
1107	28	2.886181411008259
1107	29	1.2501955587500149
1107	30	0.765125592761656
1107	31	0.8327467252168717
1107	32	0.2175046589030174
1107	33	-0.08773869484129193
1107	34	-0.6338571447133745
1107	35	-0.27751490736191187
1107	36	-0.253784642932942
1107	37	0.1446102221069765
1107	38	0.02533272250096985
1107	39	-0.5056077961664798
1107	40	-0.11439419218154256
1107	41	-0.34472519558052284
1107	42	-0.42062186174789673
1107	43	-1.0730863706902554
1113	1	-7.906345101773141
1113	2	-2.227576562204927
1113	3	3.3419694218410037
1113	4	2.3671568248164974
1113	5	-18.027530005531993
1113	6	-8.959028942144549
1113	7	-1.6829734239284413
1113	8	1.3523082154742951
1113	9	-20.707040159576668
1113	10	-21.494972039257117
1113	11	-10.947993109037036
1113	12	-21.958427242621497
1113	13	-11.007094385218371
1113	14	-0.4407749868446942
1113	15	0.7355293286926177
1113	16	1.0917455012898323
1113	17	0.346635822728409
1113	18	-0.4364710073970599
1113	19	0.09303413735536736
1113	20	0.36343324441347136
1113	21	0.5726899463718027
1113	22	1.1651679652085534
1113	23	1.3564655388652014
1113	24	0.2433980901871564
1113	25	0.6276947177160892
1113	26	12.856810828062304
1113	27	8.341491688557436
1113	28	3.961558295430372
1113	29	1.6762733159571042
1113	30	0.04547470675266396
1113	31	0.19720057340518338
1113	32	1.0209957988130896
1113	33	0.8667706942092295
1113	34	0.6779651245275673
1113	35	-0.24974447625903196
1113	36	0.5959905488152728
1113	37	0.3942796217367004
1113	38	0.2726217994647371
1113	39	1.6028291252828737
1113	40	1.715016016757886
1113	41	0.2723073354959098
1113	42	0.828518577289401
1113	43	0.7766888210579594
1114	1	-2.9741006976594093
1114	2	-0.73453360999682
1114	3	-2.499530880639334
1114	4	-1.3043495272706167
1114	5	0.7770676956174327
1114	6	0.7227617414610172
1114	7	1.4325740654847472
1114	8	0.9276258198300127
1114	9	-6.155316021645632
1114	10	1.1257176159152955
1114	11	1.6261145739635694
1114	12	-21.958427242621497
1114	13	-8.987735643536581
1114	14	-0.5944349989439068
1114	15	-27.527628566044225
1114	16	-13.9572563135922
1114	17	-6.7925051331094615
1114	18	-3.441915653495066
1114	19	-1.888817042317953
1114	20	-1.603898878998507
1114	21	-0.6692943246505862
1114	22	-0.4694357855476454
1114	23	0.43390050559236215
1114	24	0.0951827241859462
1114	25	-0.17871786546600532
1114	26	12.22462692001633
1114	27	7.02630717555078
1114	28	2.4558111689935913
1114	29	0.8988988452977011
1114	30	0.5324680521852159
1114	31	0.05442985350197915
1114	32	-0.7872316664016736
1114	33	-0.5488372912717168
1114	34	-1.2052774647041318
1114	35	-0.7143542766220037
1114	36	-0.5498049744757125
1114	37	-0.6281039233328656
1114	38	-0.5591991321747116
1114	39	-0.8599657930474365
1114	40	-0.6739736989105936
1114	41	-0.6889751811405063
1114	42	-0.5653108237995284
1114	43	-0.41508371372727737
1115	1	-2.2129812573795498
1115	2	-0.7035491022506939
1115	3	1.6508939069668642
1115	4	-3.2758637701491757
1115	5	4.581165646641921
1115	6	2.468888677992748
1115	7	2.859360672181399
1115	8	1.4598597028884832
1115	9	-20.707040159576668
1115	10	-21.494972039257117
1115	11	-9.27293590079905
1115	12	-21.958427242621497
1115	13	-8.261098961877412
1115	14	-0.5849397465700932
1115	15	0.9071540426514275
1115	16	-27.75035976186806
1115	17	-14.998901934708663
1115	18	-5.969651785429097
1115	19	-3.071725588045087
1115	20	-1.7853081743508312
1115	21	-0.36094849756413083
1115	22	-0.6128640714047151
1115	23	0.34044723222904594
1115	24	0.4150228041459627
1115	25	0.17460547286482608
1115	26	12.900289088931867
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1115	28	2.6137322084738486
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2206	2	0.07641617826694258
2206	3	-0.5509525382315914
2206	4	2.188390944598712
2206	5	1.1149382340324365
2206	6	-0.5470507207289437
2206	7	0.6999666849644797
2206	8	-0.33008742880882735
2206	9	2.2022157387899384
2206	10	1.7282584507610323
2206	11	1.0284134245382823
2206	12	3.1422987101915716
2206	13	1.6725789360157464
2206	14	0.1771923452787192
2206	15	1.9161100909430715
2206	16	1.6716003107272215
2206	17	0.9010822837084476
2206	18	0.3747812612054773
2206	19	0.15201780341707405
2206	20	0.08847861646428612
2206	21	0.05726344909412262
2206	22	0.1642605241922226
2206	23	1.0579174444913306
2206	24	0.6136340248514003
2206	25	0.2393099627251587
2206	26	-7.450811676474899
2206	27	-4.314588166252001
2206	28	-1.6954290003954
2206	29	-0.6812584444784662
2206	30	-0.22947992119651417
2206	31	-0.062327557266321776
2206	32	-0.04887715944462201
2206	33	0.0936309482927129
2206	34	0.10174007915551897
2206	35	0.26114481593698
2206	36	0.2856457212290664
2206	37	0.1365663730978639
2206	38	0.09567080127961702
2206	39	0.02025199279648149
2206	40	0.10430821276514735
2206	41	0.1216721267844818
2206	42	0.1016583232059105
2206	43	0.20681586280024788
2207	1	0.8918831421038789
2207	2	-4.766398865487886
2207	3	-1.7891405312595694
2207	4	-0.5247660516816772
2207	5	0.0021729647650374773
2207	6	-2.118799656058048
2207	7	-0.3719781138711191
2207	8	0.24548174335339823
2207	9	1.1563261770569966
2207	10	1.679285386485457
2207	11	1.533090788826435
2207	12	1.7425628563884032
2207	13	-0.8037593148692324
2207	14	0.44562417938772114
2207	15	1.9436585626686451
2207	16	1.8654818222903558
2207	17	1.0731600540989135
2207	18	0.41011575654248844
2207	19	0.23279442917402093
2207	20	0.414605730083089
2207	21	0.25773424027487124
2207	22	0.3033649428010534
2207	23	1.1203008697667087
2207	24	0.5089530667374405
2207	25	0.3402533419995706
2207	26	6.969682115191016
2207	27	4.255509406118666
2207	28	1.5892810677076028
2207	29	0.5522503873484581
2207	30	0.44422405537174114
2207	31	0.2161687860159205
2207	32	0.3154199780731233
2207	33	-0.09987860229936985
2207	34	-0.1712271631222464
2207	35	0.11971878585665507
2207	36	0.19463567648593028
2207	37	-0.1660121969188495
2207	38	0.07585264886546383
2207	39	-0.21749395965720453
2207	40	4.771944546035911E-4
2207	41	-0.160626484201849
2207	42	-0.1264579729972084
2207	43	0.2406804833820857
2208	1	-24.169502996509983
2208	2	-11.102349701950843
2208	3	2.7303541768319306
2208	4	3.04047805893628
2208	5	7.420745856536971
2208	6	2.9756353046794075
2208	7	-25.314552371296863
2208	8	-11.658581076721712
2208	9	3.6377874266302292
2208	10	3.0567520986739183
2208	11	-21.316414161668614
2208	12	-6.958427242621497
2208	13	1.6789310231300867
2208	14	0.18717419645839328
2208	15	1.9206472960247396
2208	16	1.6289505829595257
2208	17	0.7132419933273226
2208	18	0.35118779478079887
2208	19	0.5939415783717052
2208	20	-0.08665749968816527
2208	21	0.10082061787814922
2208	22	0.4501044443374127
2208	23	1.8591878619142008
2208	24	1.1181712299330755
2208	25	0.7982936287868654
2208	26	-22.143189171937696
2208	27	-11.9162215600814
2208	28	-2.9694761873282474
2208	29	-0.8482275914839121
2208	30	0.3830427648288932
2208	31	-0.30189198557847874
2208	32	0.7415039657822362
2208	33	1.2097833983834576
2208	34	0.5418489720774815
2208	35	1.0097836544124732
2208	36	0.9099651404668165
2208	37	0.7627006743682827
2208	38	0.14195029311265017
2208	39	0.726241103504286
2208	40	0.8547619332733163
2208	41	0.609875393572139
2208	42	1.020896072752194
2208	43	1.2285944471922576
2209	1	-4.643861970868958
2209	2	-4.656726359775778
2209	3	-2.2214583784289026
2209	4	-3.336621852646388
2209	5	1.6263161483141602
2209	6	-5.069015446867898
2209	7	-4.98121903796353
2209	8	-7.625424578047973
2209	9	-3.7070401595766675
2209	10	-0.8924079366930151
2209	11	-2.021542366796819
2209	12	-0.5994528836471389
2209	13	-1.9612104445975902
2209	14	0.6084827729393822
2209	15	1.921089382673724
2209	16	1.9419479304396319
2209	17	1.3021014097729449
2209	18	0.3246625958418079
2209	19	0.23540930604632848
2209	20	-0.472599144250502
2209	21	0.02478171425303799
2209	22	-0.8597982965998128
2209	23	0.34769360904063973
2209	24	-0.7120674968573795
2209	25	-0.9134658760760885
2209	26	-3.6431891719376956
2209	27	-2.195620322238785
2209	28	-2.2820314481593726
2209	29	-1.1934972643397899
2209	30	-1.5037830530314125
2209	31	-0.9809370784166802
2209	32	-0.3561971836430473
2209	33	-0.5974668226598681
2209	34	-0.8321563329623061
2209	35	-1.8124975126962752
2209	36	-1.0780985200106414
2209	37	-1.4039659922983816
2209	38	-1.0499153125460623
2209	39	-1.2392761378750237
2209	40	-0.4361310817576296
2209	41	-1.6487452960830353
2209	42	-1.8982020704838831
2209	43	-2.410220760588466
2215	1	-3.9028363298433177
2215	2	-1.9644186674680846
2215	3	2.068285211314688
2215	4	0.186455070430533
2215	5	3.672469994468006
2215	6	2.184830706978257
2215	7	2.252114295369804
2215	8	-2.045937398560792
2215	9	-20.707040159576668
2215	10	-10.494972039257117
2215	11	-3.883080828335281
2215	12	-21.958427242621497
2215	13	-10.15095403434118
2215	14	0.31361097806758664
2215	15	-26.59429523271089
2215	16	-13.250359761868062
2215	17	-4.790206282534747
2215	18	-2.1855938144146023
2215	19	-1.3543342836972627
2215	20	-1.1751632468145985
2215	21	-0.4623977729264439
2215	22	-0.7085162453177603
2215	23	0.4810269423739726
2215	24	-0.41975980454968465
2215	25	-0.044235106845320615
2215	26	5.890144161395639
2215	27	4.1941232675048035
2215	28	1.6615582954303747
2215	29	0.2218873510448276
2215	30	0.6244220751737188
2215	31	0.3498321523525547
2215	32	0.6438028163569527
2215	33	0.26150753631448964
2215	34	0.8498949490889771
2215	35	0.4362204360216708
2215	36	0.5731835312714111
2215	37	0.029367341034948424
2215	38	0.3603410977103465
2215	39	0.2940571954583078
2215	40	0.4202791746526273
2215	41	1.084588037250299
2215	42	-0.346920019201832
2215	43	0.18721513684743485
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	5.0
25	71.0
26	670.0
27	2538.0
28	6587.0
29	13261.0
30	22498.0
31	32777.0
32	43468.0
33	49135.0
34	39358.0
35	27011.0
36	11395.0
37	5617.0
38	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.105018017025696	3.790656323267926	0.5224150682129314	94.58191059149344
2	0.9653958437292491	1.1469232673364584	97.2617649023406	0.625915986593689
3	3.16376366858279	91.1860970425175	1.6329609794622544	4.017178309437462
4	95.64286513173663	2.363509994088229	1.09247926885775	0.9011456053173982
5	2.2579042935194154	90.56492390870673	2.202128439171749	4.975043358602103
6	0.7881997925376418	0.700578379907585	97.63736522805142	0.8738565995033478
7	1.2843610844492	3.333831605116989	1.7115635768571114	93.6702437335767
8	86.75792807270527	8.500355589433688	1.855744635707466	2.8859717021535753
9	29.869930174701242	21.88910748995596	20.681062795456427	27.559899539886374
10	23.349268354151242	24.858632475939956	23.98325641469907	27.808842755209728
11	27.621060586380757	25.951028665666932	25.7447838562269	20.68312689172541
12	27.925258343751487	21.24946590495482	24.766976309917553	26.058299441376143
13	21.76762669278766	22.882945301207698	26.101083463739204	29.248344542265436
14	31.21580403748192	26.63747563046349	21.841393623042578	20.30532670901201
15	27.20817231974043	29.15099292263848	24.56748360917807	19.07335114844302
16	21.560769569594765	27.058425622954946	33.3029197080292	18.077885099421092
17	20.787640418534657	30.39523470867206	30.33393973446703	18.483185138326256
18	21.15172999229717	31.70814141763476	27.701098831999747	19.43902975806832
19	21.951066178926315	32.682401662822635	24.293241443260893	21.073290714990158
20	21.79154207939271	30.794044957506078	26.939926996114057	20.474485966987153
21	20.55149760124476	28.777597472761062	28.965804476890618	21.70510044910356
22	20.93930618804198	27.864066607205302	28.928084492764444	22.268542711988275
23	22.059680084317634	28.968337302231962	28.86769501424445	20.104287599205957
24	21.97346241950123	31.039617770827522	27.20396688499727	19.782952924673978
25	22.134558185040095	31.433713021645772	27.44953969831871	18.982189094995423
26	20.203163002311143	32.40141330784216	28.66364967389858	18.731774015948112
27	20.86694459397335	32.218087802501344	28.68924924264154	18.22571836088376
28	20.865994774169465	32.499950885051376	29.030470914127427	17.60358342665174
29	21.825504410522388	32.05752342783049	28.070961277774504	18.046010883872615
30	21.5590202195626	32.67349296283781	27.099164656236002	18.668322161363584
31	22.083086123367924	33.09182065719214	26.169417737036703	18.655675482403232
32	21.331043939852343	34.11143897810277	26.407132220331857	18.150384861713036
33	21.82808331401494	33.07885441264877	26.6637066956901	18.429355577646195
34	20.729095859838825	34.30829014526123	26.731288333916158	18.231325660983785
35	22.354984342277422	33.5138129796037	27.2900155987851	16.84118707933377
36	22.21078398629507	33.431693430829014	26.894348681961596	17.463173900914317
37	21.85008663808854	33.684338741174116	26.451531784980375	18.014042835756975
38	21.75421501176785	34.17312686880911	26.01303696951361	18.059621149909432
39	23.195039822087406	33.10478690173551	25.966279905857203	17.733893370319873
40	22.026899063679977	33.389651365188385	26.304188096987506	18.27926147414413
41	22.09173028639684	32.23211935231644	27.59256130479712	18.083589056489604
42	23.324309351019814	32.62346418762549	26.44288762195146	17.60933883940324
43	24.374968075534266	31.451394264204914	26.660563363679586	17.513074296581234
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	7.0
9	11.0
10	31.0
11	51.0
12	51.0
13	114.5
14	178.0
15	310.5
16	443.0
17	693.5
18	944.0
19	944.0
20	1296.0
21	1648.0
22	2119.5
23	2591.0
24	3382.0
25	4173.0
26	4173.0
27	5357.5
28	6542.0
29	8135.5
30	9729.0
31	11400.0
32	13071.0
33	13071.0
34	14681.5
35	16292.0
36	18316.5
37	20341.0
38	21422.0
39	22503.0
40	22503.0
41	22866.0
42	23229.0
43	23299.5
44	23370.0
45	22872.0
46	22374.0
47	22374.0
48	21858.0
49	21342.0
50	19983.5
51	18625.0
52	17308.0
53	15991.0
54	15991.0
55	14059.5
56	12128.0
57	9996.0
58	7864.0
59	6464.0
60	5064.0
61	5064.0
62	4082.5
63	3101.0
64	2399.0
65	1697.0
66	1216.5
67	736.0
68	736.0
69	496.0
70	256.0
71	194.5
72	133.0
73	90.0
74	47.0
75	47.0
76	35.5
77	24.0
78	13.5
79	3.0
80	3.0
81	3.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.11905369989823464
2	0.0
3	0.0
4	1.6341397289661976
5	3.4894914481723487
6	0.0011787495039429171
7	0.11591036788772018
8	0.0
9	0.34340902214870317
10	0.2196403242346969
11	0.1728832605782945
12	0.6848534617908348
13	1.3233427764265817
14	0.03575540161960181
15	0.45735480752985186
16	0.09233537780886183
17	0.0
18	0.021610407572286813
19	0.0
20	0.0
21	0.0
22	0.0
23	0.8361263147968425
24	0.0
25	0.0
26	35.05640316376367
27	0.0015716660052572226
28	7.858330026286113E-4
29	7.858330026286113E-4
30	3.9291650131430565E-4
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	254507.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.52416370570221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.2203440631601	61.66838003535432
2	8.470600294140434	12.117052042473391
3	2.358426359512129	5.060534190767664
4	0.9323501410212283	2.6674225646974743
5	0.4970027721499049	1.7773853818718803
6	0.3083992807683992	1.323480038663988
7	0.2040253670258484	1.0214920625890929
8	0.14957892195503775	0.8558805840667658
9	0.12025727323817079	0.7741170808099458
>10	0.6837234571895503	8.864623343564494
>50	0.03797839140469279	1.896267813450435
>100	0.017313678434492302	1.9733648616905728
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAGATCACCAAGATTTGTGCCAAAGCTTAAAGAGTGAGTTAG	334	0.1312341114389781	No Hit
ATAGATAGCATGTCGACTCACTATAGTGAGTCGTATTACCGGC	270	0.10608745535486254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.4365302329601936	0.0	0.0	0.0	0.0
4	0.4365302329601936	0.0	0.005893747519714585	0.0	0.0
5	0.4365302329601936	0.0	0.005893747519714585	0.0	0.0
6	0.4365302329601936	0.0	0.005893747519714585	0.0	0.0
7	0.4365302329601936	0.0	0.005893747519714585	0.0	0.0
8	0.4365302329601936	0.0	0.005893747519714585	0.0	0.0
9	0.4365302329601936	0.0	0.005893747519714585	0.0	0.0
10	0.4365302329601936	0.0	0.005893747519714585	0.0	0.0
11	0.4365302329601936	0.0	0.005893747519714585	0.0	0.0
12	0.4365302329601936	0.0	0.009429996031543337	0.0	0.0
13	0.4365302329601936	0.0	0.010608745535486254	0.0	0.0
14	0.4365302329601936	0.0	0.049114562664288215	0.0	0.0
15	0.4365302329601936	0.0	0.08094079927074697	0.0	0.0
16	0.4365302329601936	0.0	0.14027119096920712	0.0	0.0
17	0.4365302329601936	0.0	0.1595240995336081	0.0	0.0
18	0.4365302329601936	0.0	0.1866353381242952	0.0	0.0
19	0.4365302329601936	0.0	0.2428223978122409	0.0	0.0
20	0.4365302329601936	0.0	0.3017598730093868	0.0	0.0
21	0.4365302329601936	0.0	0.3571610996947039	0.0	0.0
22	0.4365302329601936	0.0	0.38152192277619085	0.0	0.0
23	0.4365302329601936	0.0	0.4050969128550492	0.0	0.0
24	0.4365302329601936	0.0	0.5382956068005988	0.0	0.0
25	0.4365302329601936	0.0	0.6813172132790061	0.0	0.0
26	0.4365302329601936	0.0	0.7378971894682661	0.0	0.0
27	0.4365302329601936	0.0	0.8667738018993584	0.0	0.0
28	0.4365302329601936	0.0	1.0781628796064548	0.0	0.0
29	0.4365302329601936	0.0	1.217255321071719	0.0	0.0
30	0.4365302329601936	0.0	1.7512288463578605	0.0	0.0
31	0.4365302329601936	0.0	2.3732156679384064	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATTAC	20	3.063386E-4	53.2323	22
GTATTGT	65	1.8189894E-12	44.470314	24
TTGTGTC	25	9.204008E-4	42.585842	23
ATTGTGA	70	3.6379788E-12	41.29386	26
TTGGTAT	80	1.4551915E-11	36.597206	21
TGGTATT	80	1.4551915E-11	36.597206	22
CTCGTCT	40	5.4691554E-8	35.513596	5
ATCAGCG	20	0.0022491072	35.513596	5
ATTGTTC	20	0.0022491072	35.513596	5
ATCGTGT	405	0.0	35.513596	5
CTCGCGT	20	0.0022491072	35.513596	5
CTCGCAT	25	1.5664879E-4	35.513596	5
ATCGGCC	170	0.0	35.513596	5
TTCGCTA	20	0.0022491072	35.513596	5
ATCGCTG	290	0.0	35.513596	5
AAATCGC	60	1.8189894E-12	35.506336	3
AAATCGA	50	2.7830538E-10	35.506336	3
ATATCGT	35	7.7419463E-7	35.506336	3
GTTCGTT	25	1.568373E-4	35.506336	4
GTTCGCC	25	1.568373E-4	35.506336	4
>>END_MODULE