FastQCFastQC Report
Thu 26 May 2016
SRR2099026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946561
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT1150.036.57087726
CTGCACG45950.035.532914
TGCACGG13650.035.51875
GGCATAC1200.035.47484622
TCTGCAG252200.035.083873
TAACGCT301.2286469E-534.94838310
TGCAACG12350.034.9276355
CTGCCGA4200.034.800494
TCTGCAA179550.034.7558173
CTGCAAC36800.034.535244
CTGCAGG82350.034.5020984
GCCCGTA251.8711433E-434.468596
TGCACGA10500.034.460975
CTGCAGT67250.034.377044
CTGCAAG54550.034.342254
CTGCAGC56900.034.333584
TGCAGCG17300.034.3098565
TGCCGCG1300.034.2781565
CTGCATG45450.034.276654
CTGCATA39650.034.2556764
Produced by FastQC (version 0.11.5)