FastQCFastQC Report
Thu 26 May 2016
SRR2099025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008655
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT11800.035.5274055
ATTCGAC176500.035.4166953
ATTCGAG210500.035.3755073
TTCGACG47850.035.3417854
TTCGACC47800.035.3415954
TTCGAGC59900.035.230854
TTCGATA44800.035.2101974
TTCGAGT59600.035.199554
TTCGATG54550.035.16924
TCGACCG13600.035.135565
TTCGACA44000.035.1236884
GCCTTCG37550.035.0747341
TCGACCT11600.035.0685
TTCGACT49800.035.0636944
TCGACGC14850.035.0489235
TCGATAC10800.035.0339665
TTCGAAC38950.035.0257344
TCGAGCG17400.035.0169565
TTCGAAG51850.035.0135084
GCGTTCG23150.034.992961
Produced by FastQC (version 0.11.5)