FastQCFastQC Report
Thu 26 May 2016
SRR2099023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279151
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTACTCGAGACGATCTCGAACTATACCACAGTGACTACG2830.10137882364741663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG200.002093860436.0361984
GATACGC309.932282E-636.0361984
GATACGA200.002093860436.0361984
GGTACGC13600.036.0361984
GGTACCG18100.036.0361984
GGTAACG700.036.0361984
GGGTACG356.897553E-736.0361983
CGTTACG453.3705874E-936.0361983
GGTGCAT356.897553E-736.0361984
GTCCCGG200.002093860436.0361985
CGATACG850.036.0361983
AGTACGC200.002093860436.0361984
GTACGGG3150.036.0361985
GTACGCA2650.036.0361985
AGGTACG551.6370905E-1136.0361943
GATACCC251.4378835E-436.0361944
GGTACGT11950.036.0361944
GGTACGG15200.036.0361944
GTACCCC5350.036.0361945
GGTAACT251.4378835E-436.0361944
Produced by FastQC (version 0.11.5)